(SAV) User Guide 15020619 D - Illumina

More documents

Recommendations

Info

} Signal to noise—The signal to noise ratio is calculated as mean called intensity divided by standard deviation of non called intensities. } %Q>=30—The percentage of bases with a quality score of 30 or higher, respectively. This chart is generated after the 25 th cycle, and the values represent the current cycle. } %PF—The percentage of clusters passing filter The buttons above the data table allow you to do the following: } —Select all rows. } —Copy selected rows to clipboard. } —Sort columns in ascending, descending, or custom fashion. } —Create custom scatter or box plot. } —Choose columns to display. } —Filter columns, or clear filter. } —Move thumbnail selection up or down. Imaging Tab Sequencing Analysis Viewer v1.8 User Guide 18
Summary Tab The Summary link leads to tables with basic data quality metrics summarized per lane and per read. All the statistics are given as means and standard deviations over the tiles used in the lane. The following metrics are displayed in the top table, split out by read and total: } Yield Total—The number of bases sequenced. } Projected Total Yield—The projected number of bases sequenced at the end of the run. } Yield Perfect—The number of bases in reads that align perfectly, as determined by a spiked in PhiX control sample. If no PhiX control sample is run in the lane, this chart is not available. } Yield =30—The percentage of bases with a quality score of 30 or higher, respectively. This chart is generated after the 25 th cycle, and the values represent the current cycle. } Yield—The number of bases sequenced. } Cycles Err Rated—The number of cycles that have been error rated starting at cycle 1. } Aligned—The percentage of the sample that aligned to the PhiX genome. } Error Rate—The calculated error rate, as determined by the PhiX alignment. Subsequent columns display the error rate for cycles 1–35, 1–75, and 1–100. } Intensity Cycle 1—The average of the four intensities (one per channel or base type) measured at the first cycle averaged over filtered clusters. 19 Part # 15020619 Rev D
Page 1 and 2: Sequencing Analysis Viewer User Gui
Page 3 and 4: Revision History Part # Revision Da
Page 5 and 6: Setting Up Sequencing Analysis View
Page 7 and 8: If the Sequencing Analysis Viewer d
Page 9 and 10: Loading Data To load data, perform
Page 11 and 12: Figure 6 Status Pane Flow Cell Char
Page 13 and 14: Figure 8 Data by Cycle Plot You can
Page 15 and 16: } The cutoff slider allows you to d
Page 17: Imaging Tab The Imaging tab lists d
Page 21 and 22: Tile Status Tab The Tile Status tab
Page 23 and 24: Figure 16 In-Line Controls in a Suc
Page 25 and 26: Inefficient Adapter Ligation due to
Page 27 and 28: Notes
Page 29: Illumina San Diego, California 9212

(SAV) User Guide 15020619 D - Illumina

Create successful ePaper yourself

Delete template?

Save as template?