Beginner's Guide to BLAST

Example 2 Conclusions
Homologs to leptin have been identified in
many other species besides mice.
35
Options for Advanced Blasting: Nucleotide
Many of the algorithm parameters can be
adjusted:
Word Size
Scoring System and Gap Costs
Expect Threshold
Filtering: Filtering can eliminate statistically
significant but biologically uninteresting
reports from the blast output
Low Complexity Regions (Ex: AAATAAAAAAAAT)
Species Specific Repeats
36