Phd Thesis 33 - eCommons@USASK - University of Saskatchewan

Figure 4.3 Expression of Mamestra configurata genes encoding peritrophic matrix
proteins……….......................................................................................................................58
Figure 4.4 Alignment of Mamestra configurata serine proteases (McSP) associated with the
peritrophic matrix. .................................................................................................................60
Figure 4.5 Alignment of Mamestra configurata peritrophic matrix aminopeptidases (APNs)
with those from H. armigera. ...............................................................................................61
Figure 4.6 Alignment of Mamestra configurata peritrophic matrix associated
carboxypeptidases with Bos taurus BtCPA and BtCPB........................................................65
Figure 4.7 Alignment of Mamestra configurata insect intestinal lipases (McIILs) associated
with the peritrophic matrix.....................................................................................................67
Figure 4.8 Alignment of Mamestra configurata peritrophic matrix proteins 363-100-1 and
530-224-1 with L. sticticalis Lsti99 and Lsti201...................................................................71
Figure 5.1 Separation of strongly associated peritrophic matrix proteins using one
dimensional SDS-PAGE denaturing gel electrophoresis.......................................................89
Figure 5.2 Amino acid sequence of the Mamestra configurata insect intestinal mucins
(McIIMs)................................................................................................................................91
Figure 5.3 Mamestra configurata insect intestinal mucin (McIIM) gene expression in
feeding, starved and molting larvae. .....................................................................................93
Figure 5.4 Localization of Mamestra configurata McIIM2. .................................................94
Figure 5.5 Localization of Mamestra configurata McIIM4. .................................................95
Figure 5.6 One dimensional (1D) and two dimensional (2D) gel electrophoresis of loosely
(LOOSE) and/or strongly (STRONG) associated peritrophic matrix (PM) proteins from
5 th instar Mamestra configurata larvae..................................................................................96
Figure 5.7 Alignment of amino acid sequences of the lepidopteran chitin deacetylases
(CDAs) that have been demonstrated or proposed to be associated with the peritrophic
matrix. ....................................................................................................................................97
Figure 5.8 Phylogenetic analysis of insect chitin deacetylases (CDAs) and comparison of
their putative signature motifs with those of active microbial CDAs....................................99
Figure 5.9 Expression of Mamestra configurata McCDA1...................................................101
Figure 5.10 Localization of Mamestra configurata McCDA1 by western blot analysis.......101
Figure 5.11 Demonstration of chitin deacetylase (CDA) activity. ........................................103
xvii