Phd Thesis 33 - eCommons@USASK - University of Saskatchewan

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"Science is the most genuine guide in life." Mustafa Kemal Atatürk i
"Bugs are not going to inherit the earth. They already own it. It is time to make peace with the landlord." Thomas Eisner ii
Page 1: THE MOLECULAR ARCHITECTURE OF MAMES
Page 5 and 6: DEDICATION This Ph.D thesis is dedi
Page 7 and 8: ACKNOWLEDGMENTS I owe my deepest gr
Page 9 and 10: 2.1.1 Mamestra configurata ........
Page 11 and 12: 4.3.3.3.1 Alkaline phosphatase ....
Page 13 and 14: 5.3.3.4 Localization...............
Page 15 and 16: 8.2.1 Preparation of dsRNA.........
Page 17 and 18: LIST OF FIGURES Figure 1.1 The alim
Page 19 and 20: Figure 5.12 Two dimensional gel ele
Page 21 and 22: LIST OF ABBREVIATIONS Ac...........
Page 23 and 24: Ld ................................
Page 25 and 26: wt.................................
Page 27 and 28: 1.2 The Peritrophic Matrix The pres
Page 29 and 30: Now that many PM proteins have been
Page 31 and 32: 1.2.3.1 Mechanical protection and s
Page 33 and 34: 1.2.3.3 Compartmentalization of dig
Page 35 and 36: the anterior midgut and disintegrat
Page 37 and 38: of larvae reared on the leaves of r
Page 39 and 40: 2. GENERAL MATERIAL AND METHODS Thi
Page 41 and 42: Blots were prehybridized, hybridize
Page 43 and 44: samples were mixed with loading buf
Page 45 and 46: over the m/z range 50 to 1900. LC-M
Page 47 and 48: acetylglucosamine (UDP-GlcNAc) as t
Page 49 and 50: For SEM analyses, PMs were dissecte
Page 51 and 52: Chapter 2) was conducted using tota
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significant difference was detected
Page 55 and 56:
The PMs from all three stages studi
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amino acids (Figure 3.4) including
Page 59 and 60:
1 McCHS-B MATKPKTPGFTGLGDDSEDESEYTP
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36 1 70 AiCHI MKAILATLAVLAVVTTAIEAD
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38 1 70 AiNAG MWLQKYSLCAVYITLLSVICV
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3.3.4 Expression Analysis of the Ge
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Figure 3.10 Expression of Mamestra
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H. virescens (Ryerse et al., 1992)
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3.4.3 Tissue Specific Expression of
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(Chamankhah et al., 2003), McSerpin
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4. SURVEY AND PRELIMINARY CHARACTER
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Table 4.1 RT-PCR primers used in ex
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Table 4.2 Proteins identified from
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Figure 4.2 Two dimensional gel elec
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Figure 4.3 Expression of Mamestra c
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Trypsins Chymotrypsins Elastase 57
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(Figure 4.5 continued) 351 420 McAP
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(Figure 4.5 continued) 1051 1102 Mc
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4.3.3.2.3 Insect intestinal lipases
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4.3.3.2.5 α-Amylase Mascot analysi
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4.3.4.3 REPAT Mascot analysis ident
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Appendix A). All proteins were pred
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eported to be restricted to midgut
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and Ellar, 2007; Angelucci et al.,
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the M. configurata PM. McALP1 is pr
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larvae; whereas HMG176 is expressed
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4.4.4 Proteins without Orthologs Th
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5.2 Material and Methods 5.2.1 Rapi
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Table 5.2 (continued) Primer 1 Sequ
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uffer (without β-mercaptoethanol)
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5.3.2 Insect Intestinal Mucins (McI
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proline (9.3%), histidine (11.1%),
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molting larvae (Figure 5.4B). A low
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Figure 5.6 One dimensional (1D) and
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(Figure 5.7 continued) 421 429 BmCD
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meliloti (RhiNODB) and Colletotrich
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5.3.3.5 Demonstration of chitin dea
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similar colour changes, indicating
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5.3.4.3 Gene expression Expression
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transfer to a gelatin impregnated p
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produced and stored in secretory ve
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The results of CDA activity assays
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catalytically active. Of the 10 lip
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6. SURVEY, CHARACTERIZATION AND EVO
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PAD, conserved aromatic amino acids
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6.2.5 Antisera Production, Protein
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McPPAD1 MIAKFLTTVL LLNVVLTAEI PQKNA
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with the 951 bp cDNA. The McPPAD2 p
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The anti-rMcPPAD1 antiserum reacted
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Four types of PPADs were identified
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Figure 6.7 (legend is on the next p
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Figure 6.8 Model showing the propos
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Figure 6.9 Modeling of Mamestra con
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6.4.1.2 Expression Genes encoding P
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Table 6.4 (continued) Species Prote
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however, this is not present in McP
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that bonds could form between C2 an
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to form a bond with C3 in the eight
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Mamestra configurata nucleopolyhedr
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solution. In parallel, PMs without
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Figure 7.1 Analysis of strongly ass
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Figure 7.3 Analysis of Mamestra con
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kDa representing the degradation pr
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8. TARGETING GENES INVOLVED IN PERI
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Table 8.1 RT-PCR primers used for a
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50 µl 0.1% Triton-100 in nuclease
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Figure 8.1 RT-PCR analysis to detec
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clear as the same dosages and dsRNA
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RNAi to be initiated by dsRNA feedi
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2003), which could inhibit the acti
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in vitro (Cho et al., 2000), which
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glycan, two GlcNAc residues are lin
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The current study is also the first
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TcCHS2 are specialized for synthesi
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Blair, D.E., Hekmat, O., Schuttelko
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Chapman, R.F., 1985. Structure of t
Page 207 and 208:
Dong, D-J., He, H-J., Chai, L-Q., W
Page 209 and 210:
Filho, B.P.D., Lemos, F.J.A., Secun
Page 211 and 212:
Grossi de Sa, M.F., Chrispeels, M.J
Page 213 and 214:
Huang, X., Madan, A., 1999. CAP3: A
Page 215 and 216:
Kim, M.G., Shin, S.W., Bae, K.S., K
Page 217 and 218:
Lepore, L.S., Roelvink, P.R., Grana
Page 219 and 220:
novel, very large fluorescent lipoc
Page 221 and 222:
Palli, S.R., Locke, M., 1987. Hemol
Page 223 and 224:
Ponnuvel, K.M., Nakazawa, H., Furuk
Page 225 and 226:
Schorderet, S., Pearson, R.D., Vuoc
Page 227 and 228:
Strobl, S., Maskos, K., Betz, M., W
Page 229 and 230:
Tomoyasu, Y., Miller, S.C., Tomita,
Page 231 and 232:
Wang, S., Jayaram, A.S., Hemphala,
Page 233 and 234:
Zhou, H-X., Tan, X-M., Li, C-Y., Wa
Page 235 and 236:
Appendix A. (continued) Protein Acc
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Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
218 Appendix B. Amino acid composit
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220 Appendix D. Data from serine pr
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Appendix F. Domain organization and
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Appendix F. (continued) Spodoptera
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Appendix F. (continued) The colour
Page 253 and 254:
Appendix G. (continued) 27. McPM1 C
Page 255 and 256:
Appendix G. (continued) 81. HaIIM4
Page 257 and 258:
Appendix G. (continued) 135. SeCBP6
Page 259 and 260:
234 Appendix I. Tandem repeats with
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