utilizing proteomics to characterize hair and hair damage

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FURTHER MS FOLLOWING TRYPTIC DIGESTION • Full view of protein loss with tryptic digest for bleached hair reveals more than just keratin 31 but also other structural keratins within the cortex Keratin Fragment +/- Tryptic Digest Acidic/Basic Cortex/Cuticular Origin Keratin 31 - Acidic Cortex Keratin 81 + Basic Cortex Keratin 33a + Acidic Cortex Keratin 33b + Acidic Cortex Keratin 83 + Basic Cortex Keratin 85 + Basic Both Keratin 86 + Basic Cortex
2D GELS CONFIRM THE LOSS OF SPECIFIC PROTEINS WITH BLEACHING • 2D gels of hair samples pre- and post-bleaching confirms the loss of keratins detected in water extracts, as well as keratin-associated proteins kDa 188 98 4 Keratin 86 Virgin pH Keratin 85 Bleached 7 4 pH 7 Keratin 86 (missing) Keratin 85 (less abundant) 62 49 Keratins 38 28 17 14 6 3 KAP 3.2 KAP 3.3 KAP 3.1 KAP 3.1 Keratin 31 KAP 3.1 KAP 3.2 KAP 3.3 Keratinassociated proteins (KAPs) KAP 3.2 KAP 3.3 KAP 3.1 KAP 3.1 Keratin 31 KAP 3.1 KAP 3.2 KAP 3.3 Keratin-associated proteins (KAPs) (missing)
Page 1 and 2: UTILIZING PROTEOMICS TO CHARACTERIZ
Page 3 and 4: PURPOSE/OBJECTIVES • Develop new
Page 5 and 6: METHODS: AN OVERVIEW MW pI 2D gels
Page 7 and 8: PROTEIN LOSS CORRELATES WITH A MARK
Page 9: FRAGMENTATION OF KERATIN 31 IS A BI
Page 13: CONCLUSIONS • We have developed r

utilizing proteomics to characterize hair and hair damage

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