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SUMMARY OF RESULTS AND DATASETS FOR THE<br />

NOOSA FISH HEALTH INVESTIGATION TASKFORCE<br />

AUTHOR:<br />

ADDRESS:<br />

Richard Watts – Principal Scientific Advisor<br />

Product Integrity <strong>and</strong> Plant Biosecurity<br />

Biosecurity Queensl<strong>and</strong><br />

Department <strong>of</strong> Employment, Economic Development<br />

<strong>and</strong> Innovation<br />

Floor 3, Primary Industries Building<br />

80 Ann St, Brisbane<br />

Queensl<strong>and</strong> 4000 Australia<br />

DOCUMENT:<br />

FILE:<br />

BIOSECURITY.NFHIT.10.SUM.1.4<br />

\\PIBAPPPR13\DMNFSL1\QPIF_EDOCS\44247\3A\Q<br />

PIF_EDOCS_n44247_v3A_<strong>summary</strong>_<strong>of</strong>_<strong>results</strong>_(chro<br />

nological).doc<br />

DATE: 28 May 2010<br />

PAGES: 135<br />

STATUS:<br />

Final


SUMMARY OF RESULTS FOR NFHIT<br />

EXPLANATION ................................................................................................................. 4<br />

TERMS OF REFERENCE................................................................................................. 4<br />

SYNDROMES AND EVENTS............................................................................................ 4<br />

HATCHERY AND MACADAMIA FARM DATA .................................................................. 5<br />

Agricultural <strong>and</strong> veterinary chemical use data – Sunl<strong>and</strong> Fish Hatchery ........................ 5<br />

Property data – Arbuckle farm <strong>and</strong> Sunl<strong>and</strong> Fish Hatchery............................................ 8<br />

Plans <strong>and</strong> Protocols....................................................................................................... 9<br />

November 2005 – January 2006, Event 1, Syndrome 2 sudden deaths....................... 10<br />

October 2007 – Event 2, Syndrome 3 – Spinning juveniles <strong>and</strong> Syndrome 2 sudden<br />

deaths.......................................................................................................................... 23<br />

February 2008 – Event 3 Syndrome 4 – De<strong>for</strong>mities <strong>and</strong> ill thrift.................................. 30<br />

July 2008 – Event 4, Syndrome 3 – De<strong>for</strong>mities........................................................... 31<br />

October 2008 – Event 5, Syndrome 3 – Spinning juveniles.......................................... 32<br />

November 2008 – Undefined syndrome in Horse......................................................... 40<br />

December 2008 – Event 6, Syndrome 6 – Sudden deaths........................................... 42<br />

February 2009 – Exposure estimate ............................................................................ 65<br />

July 2009 – Event 7, Syndrome – De<strong>for</strong>mities.............................................................. 68<br />

September <strong>and</strong> October 2009 – Background sampling ................................................ 72<br />

September 2009, Event 8, Syndrome – Mortality ......................................................... 78<br />

November 2009 – Event 9, Syndrome – Loss.............................................................. 81<br />

December 2009 <strong>and</strong> January 2010 ............................................................................ 109<br />

February 2010, event 10, Syndrome – Larval mal<strong>for</strong>mation, discoordination <strong>and</strong><br />

mortality ..................................................................................................................... 114<br />

February 2010 – OC Sniffer Dog................................................................................ 115<br />

March 2010, event 11, Syndrome – Growth retardation ............................................. 117<br />

ENVIRONMENTAL DATA ............................................................................................. 118<br />

Date 28 May 2010 Page 2 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

HUMAN HEALTH DATA................................................................................................ 125<br />

DOCUMENTS LIST....................................................................................................... 130<br />

Date 28 May 2010 Page 3 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

EXPLANATION<br />

A number <strong>of</strong> <strong>results</strong> have been generated <strong>and</strong> <strong>datasets</strong> disclosed during <strong>the</strong> course <strong>of</strong> <strong>the</strong><br />

Noosa Fish Health Investigation Task<strong>for</strong>ce (NFHIT). These <strong>results</strong> <strong>and</strong> related in<strong>for</strong>mation<br />

need collation prior to <strong>the</strong> preparation <strong>of</strong> <strong>the</strong> final report. The purpose <strong>of</strong> this document is to<br />

summarise <strong>the</strong> <strong>results</strong> <strong>and</strong> comment on any uncertainties <strong>and</strong> inadequacies in <strong>the</strong> reports.<br />

This <strong>summary</strong> only relates to matters relating to pesticide residues. This document is not<br />

intended to provide an interpretation <strong>of</strong> <strong>the</strong> data.<br />

The non detection <strong>of</strong> pesticides <strong>and</strong> <strong>the</strong> detection <strong>of</strong> pesticides provide equal evidence to<br />

meet <strong>the</strong> aims <strong>of</strong> <strong>the</strong> Terms <strong>of</strong> Reference <strong>of</strong> <strong>the</strong> Task<strong>for</strong>ce. However, <strong>for</strong> this <strong>summary</strong> it is<br />

not possible to summarise all <strong>the</strong> analytes which have not been detected. This <strong>summary</strong> will<br />

be limited to reporting <strong>the</strong> pesticides that are considered <strong>of</strong> significance in <strong>the</strong> investigation.<br />

The National Association <strong>of</strong> Testing Authorities (NATA) requires that all analytical reports are<br />

only reproduced in full. The documents including <strong>the</strong> analytical report <strong>and</strong> accompanying<br />

sample submission sheets are included as appendices to <strong>the</strong> report.<br />

TERMS OF REFERENCE<br />

Terms <strong>of</strong> reference are defined as follows in <strong>the</strong> Noosa Fish Health Investigation Task<strong>for</strong>ce<br />

Interim Report #1, June 2009 (document 500):<br />

1. Investigate <strong>the</strong> potential causes <strong>of</strong> <strong>fish</strong> deaths <strong>and</strong> larval de<strong>for</strong>mities at <strong>the</strong><br />

Sunl<strong>and</strong> Fish Hatchery from January 2006 with particular reference to:<br />

• appropriate aquaculture husb<strong>and</strong>ry practices at Sunl<strong>and</strong> Fish Hatchery<br />

• potential chemical contamination <strong>of</strong> ponds from agricultural practices<br />

• o<strong>the</strong>r possible causes.<br />

2. Investigate any links between <strong>fish</strong> <strong>health</strong> problems at <strong>the</strong> Hatchery with broader<br />

ecosystem <strong>and</strong> <strong>fish</strong> <strong>health</strong> concerns in <strong>the</strong> Noosa River catchment.<br />

3. Make findings or recommendations in relation to scientific sampling, analysis or<br />

experimentation to assist investigations.<br />

4. Engage with relevant stakeholders including <strong>the</strong> affected parties, industry <strong>and</strong><br />

relevant local <strong>and</strong> state government agencies.<br />

5. Present a final report to <strong>the</strong> Minister on <strong>the</strong> outcome <strong>of</strong> <strong>the</strong> investigations to<br />

minimise <strong>the</strong> risk <strong>of</strong> similar occurrences in any aquaculture facilities by February<br />

2010 with Interim reports <strong>of</strong> progress in April <strong>and</strong> September 2009.<br />

SYNDROMES AND EVENTS<br />

The following table is reproduced from <strong>the</strong> Noosa Fish Health Investigation Task<strong>for</strong>ce Interim<br />

Report #1, June 2009 (document 500). Six syndromes were identified <strong>and</strong> <strong>the</strong>ir temporal<br />

distribution was recorded. Some events occurred on a number <strong>of</strong> occasions so it is<br />

necessary to use arbitrary event numbers as well as <strong>the</strong> syndrome numbers.<br />

Table 1: Syndromes identified at Sunl<strong>and</strong> Fish Hatchery pre January 2009<br />

Date 28 May 2010 Page 4 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Syndrome<br />

Temporal Spatial Species affected<br />

distribution distribution<br />

1 Sudden deaths Nov 05 – Jan On farm Multiple<br />

06<br />

2 Sudden deaths Oct 07 On farm Silver Perch/Golden Perch<br />

3 Spinning juveniles Oct 07 & Oct On farm Silver Perch/Golden Perch<br />

08<br />

4 De<strong>for</strong>mities, ill thrift Feb 08 On farm Silver Perch/Golden Perch<br />

5 De<strong>for</strong>mities Jul 08 Noosa River Australian Bass (occurred on farm<br />

but in isolated shed with introduced<br />

water)<br />

6 Sudden death Dec 08 On farm but<br />

introduced<br />

stock<br />

Silver Perch (1 mullet)<br />

The table above does not cover <strong>the</strong> period after December 2008. The Noosa Fish Health<br />

Investigation Task<strong>for</strong>ce Draft Interim Report #2 (document 501) identifies three subsequent<br />

events as follows but does not define <strong>the</strong>ir spatial distribution:<br />

Table 2: Syndromes identified at Sunl<strong>and</strong> Fish Hatchery post January 2009<br />

Syndrome<br />

Temporal<br />

distribution<br />

1 De<strong>for</strong>mities July 2009 Mullet<br />

Species affected<br />

2 Mortality Sep 2009 Australian Bass<br />

3 Mortality Nov 2009 Golden Perch<br />

HATCHERY AND MACADAMIA FARM DATA<br />

Agricultural <strong>and</strong> veterinary chemical use data – Sunl<strong>and</strong> Fish Hatchery<br />

The veterinary records <strong>of</strong> Dr Owen Johnston (BVSc), who is <strong>the</strong> prescribing vet to Sunl<strong>and</strong><br />

Fish Hatchery, (document 603) provide <strong>the</strong> following data:<br />

Product: Chorulon 5000 5 x 5ml<br />

Use instructions: Dilute 10cc diluent I/P, I/M<br />

Australian Bass 900 iu/kg<br />

Yellow Belly 400-500 iu/kg<br />

Silver perch 200-300 iu/kg<br />

Prescribed:<br />

29/8/07 – 1 x box 5<br />

14/11/07 – 1 x box 5<br />

5/3/08 – 1 x box 5<br />

Date 28 May 2010 Page 5 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

8/12/08 – 1 x box 5<br />

Product: LHRHa (Aqualife)<br />

Use instructions:<br />

50ug/kg – 1 st dose injection<br />

25 ug/kg – 2 nd dose<br />

Prescribed:<br />

29/7/06 – 5 x 1mg<br />

9/6/09 – 2 x 1mg<br />

Product: Atropine <strong>for</strong>te 10mg/ml – 80ml exp 10/09<br />

Use instructions:<br />

Fish I/P 0.1ppm<br />

Prescribed:<br />

30/10/07<br />

BSL Accession: 09-120470. (Document 215)<br />

Submitter: Mat<strong>the</strong>w L<strong>and</strong>os<br />

Property: Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: 19/7/09-21/7/09<br />

Date received: 22/7/09<br />

Animal: mullet<br />

Follow on from previous mullet egg accession (date 13/7/09). Fur<strong>the</strong>r mullet sourced from<br />

<strong>the</strong> beachfront adjacent <strong>the</strong> mouth <strong>of</strong> <strong>the</strong> Noosa River system. Females induced with LHRHa<br />

50 IU/kg <strong>and</strong> repeat dosed with 25 IU/kg 24 hours later.<br />

The following data comes from APVMA approved labels <strong>and</strong> permits stored in <strong>the</strong> database,<br />

Infopest. All registered labels <strong>and</strong> permits are publically available.<br />

Chorulon 5000 iu<br />

Registration status: registered<br />

Use by: label or under veterinary instruction<br />

Label instructions: none <strong>for</strong> <strong>fish</strong> species produced at Sunl<strong>and</strong> Fish Hatchery<br />

Product LHRHa <strong>for</strong> Injection<br />

Registration status: unregistered<br />

Use by: Permit, PER9608<br />

Use instructions: Use in accordance with label instructions. For use by or under <strong>the</strong> direction<br />

<strong>of</strong> a veterinary surgeon<br />

O<strong>the</strong>r conditions: prescribing vet must keep a record <strong>of</strong> all treatments, recording <strong>the</strong> date,<br />

<strong>the</strong> amount <strong>of</strong> product <strong>and</strong> farm details, which is to be made available on request to state<br />

Date 28 May 2010 Page 6 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

government chemical coordinators or <strong>the</strong> APVMA. The prescribing vet must report all<br />

adverse experiences associated with use <strong>of</strong> this product to <strong>the</strong> supplier.<br />

Label Instructions: Dose 20 ug/kg bw. Males may require a reduced dose. Ensure that an<br />

adequate ratio <strong>of</strong> males to females is dosed at <strong>the</strong> same time. For most species a single<br />

dose <strong>of</strong> LHRHa is required. For o<strong>the</strong>r species, split dose <strong>of</strong> one quarter <strong>of</strong> <strong>the</strong> primary dose<br />

<strong>and</strong> three quarters (resolving dose) may be required.<br />

Atropine<br />

Registration status: unregistered<br />

Use: veterinary instruction<br />

An atropine photo was taken on 9/12/08 by Roger Chong (document 444).<br />

The Noosa Fish Health Investigation Task Force, 1 st Veterinary Report on Sunl<strong>and</strong> Fish<br />

Hatchery, For Scientific Subcommittee, 20/3/09 (document 604), Record <strong>of</strong> visit <strong>and</strong><br />

sampling – Sunl<strong>and</strong> Fish Hatchery 16-12-08 (document 605) <strong>and</strong> Sunl<strong>and</strong> Fish Hatchery –<br />

Farm inspection <strong>and</strong> Sampling 9.12.08 Report 15.12.08 (document 606) indicate that Roger<br />

Chong considered ‘chemical treatments used in <strong>the</strong> management <strong>of</strong> <strong>fish</strong> diseases – records,<br />

storage conditions <strong>and</strong> prescriptions <strong>for</strong> supply’. No documents on chemical use were<br />

collected.<br />

The Report on Sunl<strong>and</strong> Hatchery – Chemicals Used on Hatchery (document 448), Appendix<br />

1 Hatchery Chemicals (document 449) <strong>and</strong> Draft Sunl<strong>and</strong> Inspection_Chem Use –<br />

Supplement Rpt (document 450) provide <strong>the</strong> following data:<br />

Purpose<br />

L<strong>and</strong> use prior to ownership by Mrs Gilson<br />

L<strong>and</strong> use during ownership by Mrs Gilson<br />

Termite treatments<br />

Weed control<br />

Vermin control<br />

Aquaculture chemicals<br />

Chemical<br />

unknown<br />

Dairy, gladioli farm <strong>and</strong> aquaculture facility<br />

No termite or pest treatments in hatchery shed<br />

Glyphosate <strong>for</strong> grass control<br />

Brodifacoum bait<br />

1. Aqualife LHRHa <strong>for</strong> injection (induction <strong>of</strong><br />

spawning <strong>of</strong> brood<strong>fish</strong>)<br />

2. Chorulon chorionic gonadotrophin 5000iu<br />

(induction <strong>of</strong> spawning <strong>of</strong> brood<strong>fish</strong>)<br />

3. Cussons Morning Fresh dishwashing liquid<br />

(cleaning filters in hatchery operation )<br />

4. Fertilisers (superphosphate, urea <strong>and</strong><br />

ammonia <strong>for</strong> growing algae)<br />

5. Activated carbon (water filter)<br />

6. Swimming pool salt (pre-entry quarantine<br />

bath)<br />

7. Formalin (preservation <strong>of</strong> samples <strong>for</strong><br />

pathology<br />

8. Ag lime (pH adjustment <strong>of</strong> ponds)<br />

9. Herbal extract (spawning inducer – not used<br />

Date 28 May 2010 Page 7 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

in 5 years)<br />

10. Yellow powder (ei<strong>the</strong>r oxytetracycline or<br />

furazolidone – not used in 8 years)<br />

Property data – Arbuckle farm <strong>and</strong> Sunl<strong>and</strong> Fish Hatchery<br />

The macadamia farm map is document 400. The following data is taken from Distance<br />

estimates – 6/9/2009 Chemical Applications (document 438).<br />

Block treated<br />

G6<br />

G5<br />

B4<br />

Knob<br />

B2<br />

B3<br />

Reference Point on<br />

Mac Farm<br />

Sou<strong>the</strong>rn Boundary<br />

NW Corner<br />

GH Sou<strong>the</strong>rn Edge *<br />

Reference Point on<br />

Hatchery<br />

Bara Pond<br />

Plankton pond<br />

Hatchery<br />

Bara Pond<br />

Hatchery<br />

Estimates from<br />

Google Earth<br />

(Rounded to 5 m)<br />

500 m<br />

485 m<br />

435 m<br />

440 m<br />

475 m<br />

NE Cnr Bara Pond 400 m<br />

Due west <strong>of</strong> hatchery Hatchery 445 m<br />

NE Corner Bara pond 515 m<br />

Due west <strong>of</strong> hatchery Hatchery 555 m<br />

Lower eastern edge Bara pond 405 m<br />

As above Hatchery 460 m<br />

Closest edge Bara Pond 480 m<br />

Closest edge Hatchery 530 m<br />

(Eastern end)<br />

Plankton Pond<br />

Bara Pond<br />

Hatchery<br />

*Field Edge – Google Image does not show trees at this point<br />

370 m<br />

380 m<br />

425 m<br />

GPS data from Report _Sunl<strong>and</strong> Fish Hatchery_IW_290409 (document 404) taken on <strong>the</strong><br />

macadamia property on 3/12/2008 are as follows:<br />

Latitude Longitude Alt (ft)<br />

-26 18.8340 152 56.7594 41<br />

-26 18.7872 152 56.8230 45<br />

-26 18.6036 152 56.9550 67<br />

-26 18.5508 152 56.8650 215<br />

-26 18.6318 152 57.1578 149<br />

-26 18.7638 152 57.1056 156<br />

-26 18.9228 152 57.0702 108<br />

-26 18.8016 152 57.1956 116<br />

Date 28 May 2010 Page 8 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Plans <strong>and</strong> Protocols<br />

The Sampling Plan <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation Task<strong>for</strong>ce ver 5, 4 September<br />

2009 was finalised on 4/9/2009 (Document 1). An amended sampling plan, Sampling Plan<br />

<strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation Task<strong>for</strong>ce ver 5a (Document 2) was provided to <strong>the</strong><br />

Scientific Subcommittee (SSC). The document provided to <strong>the</strong> SSC was amended to<br />

remove some commercial-in-confidence in<strong>for</strong>mation. The sampling plan was supported by a<br />

sampling protocol, ‘A Protocol <strong>for</strong> Responding to Adverse Fish Health Events <strong>and</strong> Chemical<br />

Overspray/Drift Incidents at Sunl<strong>and</strong> Fish Hatchery’ (document 3) <strong>and</strong> The Qld EPA Water<br />

Quality Sampling Manual.<br />

(http://www.derm.qld.gov.au/environmental_management/water/water_quality_monitoring/m<br />

onitoring_<strong>and</strong>_sampling_manual.html).<br />

Ano<strong>the</strong>r protocol was prepared by Roger Chong, titled Sunl<strong>and</strong> Fish Hatchery Veterinary<br />

Investigation Work Instruction (document 4).<br />

Where known, <strong>the</strong> appropriate plans <strong>and</strong> protocols are identified with <strong>the</strong> sample submission<br />

summaries throughout <strong>the</strong> document.<br />

2006<br />

Intention to spray notifications<br />

Intention to spray notice 29/9/06 (Document 439)<br />

Owner <strong>of</strong> property sprayed: Roger Arbuckle<br />

Notifying: G Gilson, Lot 6 Gilson Rd<br />

Intended date: 26/9/06 to a time depending on sou<strong>the</strong>rly wind.<br />

Chemicals: carbendazim <strong>and</strong> copper<br />

Intention to spray notice 9/10/06 (Document 440)<br />

Owner <strong>of</strong> property sprayed: Roger Arbuckle<br />

Notifying: G Gilson, Lot 6 Gilson Rd<br />

Intended date: 9/10/06- 18/10/06.<br />

Chemicals: carbendazim <strong>and</strong> Supracide<br />

Intention to spray notice 6/11/06 (Document 441)<br />

Owner <strong>of</strong> property sprayed: Roger Arbuckle<br />

Notifying: G Gilson, Lot 6 Gilson Rd<br />

Intended date: 6/11/06- 18/11/06.<br />

Chemicals: carbendazim <strong>and</strong> Supracide<br />

2007<br />

Intention to spray notice 22/10/07 (Document 442)<br />

Date 28 May 2010 Page 9 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Owner <strong>of</strong> property sprayed: Roger Arbuckle<br />

Notifying: G Gilson, Lot 6 Gilson Rd<br />

Intended date: 22/10/07- 28/10/07.<br />

Chemicals: Supracide/copper/spin<br />

Intention to spray notice 18/11/07 (Document 443)<br />

Owner <strong>of</strong> property sprayed: Roger Arbuckle<br />

Notifying: G Gilson, Lot 6 Gilson Rd<br />

Intended date: 18/11/07- 10/11/07.<br />

Chemicals: Supracide/ carbendazim/copper<br />

November 2005 – January 2006, Event 1, Syndrome 2 sudden deaths<br />

Fish Health issues<br />

From <strong>the</strong> Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation (document 300), <strong>the</strong><br />

following entries occur <strong>for</strong> November 2005 to January 2006:<br />

Date Reported Event Supporting<br />

evidence<br />

Oct 2005 GG reported she could smell chemicals in <strong>the</strong> air on her 20 acres. GG Verbal<br />

Then 2-3 days later <strong>fish</strong> mortalities occurred in <strong>the</strong> <strong>fish</strong> ponds. GG recollection<br />

could not see chemicals in <strong>the</strong> air.<br />

<strong>and</strong> diary<br />

entry<br />

5/11/05 “Sprayed <strong>and</strong> <strong>fish</strong> died, silver perch in pond” GG Diary<br />

entry<br />

19/11/05 – All 11 spawnings in shed died (<strong>fish</strong> were just about ready to be GG Diary<br />

21/11/05 stocked).<br />

entry +<br />

Saratoga <strong>and</strong> SP already in ponds died.<br />

witnesses<br />

RA brought to shed to witness dead fry<br />

Water source <strong>for</strong> hatchery is a spring fed dam not far from<br />

Cooloothin Creek. During flood events <strong>the</strong> dam becomes<br />

continuous with <strong>the</strong> creek <strong>and</strong> with dam on Denise Weaver’s<br />

property. RA’s property drains to <strong>the</strong> flood plain area <strong>of</strong> Cooloothin<br />

creek <strong>and</strong> into <strong>the</strong> dam on <strong>the</strong> adjacent property <strong>of</strong> Denise Weaver.<br />

Water is pumped up to <strong>the</strong> <strong>fish</strong> hatchery <strong>and</strong> stored in besablock<br />

open top tanks adjacent <strong>the</strong> hatchery building. The tanks are partly<br />

covered by shade cloth. Fish kill in hatchery reported after a few<br />

days <strong>of</strong> spray use. Immediately after water change from block<br />

tanks<br />

23/11/05 Cleaned block tanks (water supply to <strong>fish</strong> hatchery) as was GG Diary<br />

concerned re possibility <strong>of</strong> chemicals involvement from spray drift entry<br />

8/12/05 Late am- Nigel spraying in variable winds estimated @ 40km/hr. GG Diary<br />

Asking how far does spray drift carry?<br />

entry<br />

23/12/05 Caspar gold cat<strong>fish</strong> is caught <strong>and</strong> brought to <strong>the</strong> hatchery GG Diary<br />

Date 28 May 2010 Page 10 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

entry<br />

Jan 2006 New spray rig used on RA’s property. Sprays up higher into<br />

macadamia trees than previous methods. 4 ponds <strong>of</strong> 250-300 <strong>fish</strong><br />

killed involving SP, GP, AB, mullet <strong>and</strong> Mary River Cod. Estimated<br />

GG Diary<br />

entry <strong>and</strong><br />

photos<br />

loss based on previous claim on SEQEB agreed estimate <strong>of</strong> value<br />

<strong>of</strong> breeding <strong>fish</strong> lost in power outages at $8000/brood<strong>fish</strong> = $2-2.4<br />

million.<br />

24/01/06 GG reported <strong>fish</strong> deaths at hatchery to Ian Walker (QPIF). The<br />

neighbouring macadamia farm spraying operations alleged as <strong>the</strong><br />

cause <strong>of</strong> <strong>the</strong> <strong>fish</strong> deaths.<br />

Fish deaths described as follows:<br />

Eleven spawnings including six SP <strong>and</strong> five YB<br />

Approximately 42 adult <strong>fish</strong><br />

GG advised dead <strong>fish</strong> had been discarded.<br />

Ian Walker<br />

(IW) Report<br />

Inspector visited <strong>the</strong> hatchery <strong>and</strong> advised GG in future that <strong>fish</strong><br />

should be frozen so that <strong>the</strong>y can be analysed <strong>for</strong> chemical<br />

residues.<br />

Discussions with Ms Gilson <strong>and</strong> Mr Arbuckle at <strong>the</strong> hatchery.<br />

Apply bigger droplets<br />

Change <strong>the</strong> angle <strong>of</strong> spray at <strong>the</strong> tops <strong>of</strong> trees<br />

Use <strong>of</strong> smoke fires to gauge wind direction<br />

GG stated <strong>the</strong>re had been no problems with spray drift in <strong>the</strong> past<br />

10 years. GG provided photographs <strong>of</strong> dead <strong>fish</strong> to <strong>the</strong> Inspector.<br />

27/01/06 Rang IW, DPI&F,chemical use <strong>and</strong> food safety (CUFS) program,<br />

RA, Nigel Arbuckle to advise mass mortality in multiple ponds (4)<br />

including SP, GP, Saratoga, Mary River Cod <strong>and</strong> Mullet.<br />

IW visited <strong>the</strong> property at some stage after this notification- no<br />

samples were collected. Discussion was held with RA <strong>and</strong> GG to<br />

<strong>for</strong>mulate a revised spray plan: apply bigger droplets. Change<br />

angle <strong>of</strong> <strong>the</strong> spray at <strong>the</strong> tops <strong>of</strong> <strong>the</strong> trees. Use smoke fires to<br />

gauge wind direction. George Gahler (maca farm worker) told<br />

Gwen he used double strength, which means <strong>the</strong>y would have to<br />

put less spray up in <strong>the</strong> air.<br />

GG Diary<br />

entry<br />

Spray records<br />

The following in<strong>for</strong>mation is from a compilation <strong>of</strong> <strong>the</strong> Arbuckle spray records (document<br />

423) prepared by Ian Walker.<br />

Date 28 May 2010 Page 11 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

17/10/2005 T1 Supracide 4.375l Row A2 10AM<br />

Oil 9l NW


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

in Gilson<br />

T13<br />

Start 3.10PM in Gilson<br />

E-SE occ S<br />

4.45PM end 3 rows from hatchery 10-20km/hr<br />

20/10/2005 T14 *Not Full Mix <strong>of</strong><br />

4AM knob<br />

Supracide<br />

Supracide 3.5l/2800l Direction indeterimable<br />

water<br />

Spin 1.8l


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

G1<br />

T19<br />

G2 West Side work to East<br />

Supracide 3.5l/2800l<br />

water<br />

Prilled Copper 5kg<br />

Oil<br />

9l<br />

KSw, ZnSw, 2kg each<br />

MwSu<br />

T20 Full Tank Mix +<br />

Added nutrients<br />

G2 N/NE 10-15 km/hr<br />

Get to House <strong>the</strong>n rest tank in G1 NE/E 15-<br />

25km/hr<br />

T21 1/2 fill tank. Stop<br />

to windy <strong>for</strong> G1 (E-<br />

W rows) E occ NE<br />

Spray won't<br />

penetrate<br />

canopy>30km/hr<br />

26/10/2005 T21 Fill rest <strong>of</strong> tank<br />

Finished GH NW 5-10km/hr<br />

3500l (water)<br />

Supracide 4.4l G3 + 246 in G4<br />

Oil 9l + 6 rows from gully<br />

Top 5 Nozzles Off<br />

T22<br />

3500l<br />

(Water)<br />

Supracide 4.4l 9.30AM rest <strong>of</strong> C1<br />

Oil<br />

9l<br />

Cu Oxy<br />

10.5kg<br />

Spin 2.3l<br />

Ksu, KP, ZnSu, 1kg each<br />

MnSw<br />

27/10/2005 T22 1.30PM Finally got NE ly to Spray House Block<br />

*Need SE ly. Only<br />

rows left to do<br />

short rows near<br />

Hatchery + top 1/2<br />

<strong>of</strong> 1st 4 rows near<br />

hatchery shed<br />

+top 1/2 <strong>of</strong> 1st<br />

rows near<br />

Hatchery Shed<br />

R1-12 Fin at 2.30PM<br />

Date 28 May 2010 Page 14 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

+1st 4 rows from<br />

East Side <strong>of</strong> G2<br />

Next to my House<br />

8/11/2005 T1 3500l 6.45am A2 S-SW


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

T13<br />

T14<br />

G1 South Side<br />

G1 Last? A29 Row<br />

Then got A268? On Northside<br />

<strong>of</strong> G1 & Work South<br />

11/11/2005 T15 3500l 6.35AM Finn G1 NE


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

Make up 1/2 tank<br />

End T20 10.25AM<br />

mix<br />

T21 Full Tank 10.41AM Northside S/SE 15-25km/hr<br />

11.20 stopped wind changing<br />

Gilson, 1/2 way through block<br />

11.20AM occ NE<br />

16/11/2005 T21 Wind Convergence Gilson<br />

1/2 way through block<br />

North Side NE occ N/NW<br />

See Gwen (1st complaint after this spray)<br />

South Side E/SE occ NE. Wait<br />

till after lunch<br />

1.20PM. See Gwen. She thinks ok<br />

Change E-SE occ SW 5-25km/hr<br />

End T21 2PM<br />

T22 1/2 tank mix Last 3 long rows Gilson<br />

2.22PM Gilson up to Fence E-SE<br />

20km/hr steady<br />

T23 North Shield CU 3.5kg<br />

3500l<br />

(Water)<br />

Oil<br />

10l<br />

10lk, 5lmg<br />

5l Zn<br />

Started 1st A16 row on Northside<br />

Did one side. Burnie worried<br />

about drift getting to Gwens<br />

place (4.17PM) yet said it<br />

was fine earlier when wind<br />

was less favourable ie<br />

T21 & T22<br />

Don't do Gilson go to Central<br />

*Dad sprayed some Central<br />

A16's 3 rows. Didn't do I<br />

sided round A16's.? Ran out<br />

end <strong>of</strong> 2nd A16 row<br />

17/11/2005 T24 Same mix as T23 Did 1/2 2nd long row<br />

*After Gilsons<br />

agree 2<br />

<strong>of</strong> A16 & <strong>the</strong> short row. Remainder Central<br />

SE/S/SW15km/hr<br />

Water Samples<br />

taken from Fish<br />

Hatchery<br />

Date 28 May 2010 Page 17 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

B4 end T24 10.22AM<br />

1/12/2005 T1 3500l 6.45AM B4 W/NW 2km/hr<br />

(Water)<br />

Pyrethroid 1.75l<br />

Oil<br />

9l<br />

Cu Oxy<br />

10.5kg<br />

T2<br />

B2 <strong>the</strong>n A2 N/NW < 5km/hr<br />

T3<br />

9.25AM A2 thunderstorm out <strong>of</strong> nowhere<br />

5/12/2005 T3 Start from where left <strong>of</strong>f<br />

in A2 T1 & T2 redo after finished<br />

7.40AM N occ NW


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

T15 3kg each K & Mg,<br />

4.30PM G2<br />

1 kg Zn<br />

End T15 5.30PM 4.50AM 10-20km/hr<br />

7/12/2005 T15 G1 5.45AM No wind<br />

6AM NE


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

Northside instead<br />

<strong>of</strong> Southside<br />

Finished T23 1 Interrow south <strong>of</strong> electricity<br />

pole<br />

T24 North Shield 3.5kg<br />

Copper<br />

Oil<br />

9l<br />

K<br />

3kg<br />

* Gwen didn't want me to spray <strong>and</strong> most non variable we S-SE 20-30km<br />

had? when I rang her to start A16 rows Southside <strong>of</strong><br />

Block so won't <strong>and</strong> did first A16 row Northside which was<br />

1/2 way between where we'd already sprayed <strong>the</strong>n did <strong>the</strong><br />

3 rows in A16's in Central. Finished 11.20AM<br />

*5 long Rows <strong>and</strong> short rows in Gilson to do most<br />

favorable winds to guarantee no drift in Hatchery as<br />

proved from doing in <strong>the</strong>se conditions <strong>for</strong> 10 years. Gwen<br />

say no. dad not here so I did fires <strong>and</strong> ribbons show<br />

differant. Gwens fire is to close to her house<br />

20/12/2005 T1 3500l Started A2 E/S occ S


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

2.20PM G1 Northside NE/E 10-15km/hr<br />

T13<br />

3.35PM G1 occasional gusting 25km/hr<br />

5.00PM Finn 1/2 way G1<br />

22/12/2005 T13 6.00AM G1 E/NE


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

16/01/2006 T1 3500l 8.20AM Westside A2, NE/E/SE


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Tank<br />

No<br />

Chemical Rates Used Blocks/Rows/Time/Wea<strong>the</strong>r conditions<br />

Northside G1 NE oc E


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

The tarpaulins were suspended over cables laid across <strong>the</strong> ponds<br />

allowing some airspace between <strong>the</strong> pond water surface <strong>and</strong> <strong>the</strong><br />

tarpaulin. Tarps were held in place by <strong>the</strong> use <strong>of</strong> many rubber tyres<br />

<strong>and</strong> <strong>the</strong> cables were anchored into <strong>the</strong> ground by steel posts. The<br />

block tanks near <strong>the</strong> hatchery were also covered. Once covered<br />

chemical spray commenced. It rained overnight after <strong>the</strong> 4 ponds<br />

were uncovered. Block tanks uncovered but tarps left in pile still<br />

over tanks. Rain water dripped over tarp <strong>and</strong> into block tanks. GG<br />

had a batch <strong>of</strong> 4000 SP fingerlings in <strong>the</strong> hatchery shed <strong>and</strong> used<br />

water from <strong>the</strong> block tanks <strong>for</strong> water exchange. The block tanks<br />

have been in contact with <strong>the</strong> tarpaulins which had rainwater wash<br />

<strong>of</strong>f <strong>the</strong>m into <strong>the</strong> block tanks. 4000SP died when <strong>the</strong> when all<br />

exposed to <strong>the</strong> block tank water.<br />

[Note – Ian walker records this incident as reported on<br />

23/10/2007]<br />

In <strong>the</strong> morning GG inspected <strong>the</strong> covered ponds- no <strong>fish</strong> died but<br />

<strong>the</strong> <strong>fish</strong> looked pale <strong>and</strong> stressed <strong>and</strong> h<strong>and</strong>led poorly.<br />

GG inspected <strong>the</strong> uncovered pond <strong>and</strong> <strong>the</strong> one GP in that pond<br />

(left <strong>the</strong>re as a sentinel) died on 14/10/07(diary <strong>and</strong> dated<br />

photographed).<br />

GG reported that RA collected a water sample from <strong>the</strong> uncovered<br />

pond <strong>and</strong> RA told GG a positive result <strong>for</strong> carbendazim was found.<br />

After <strong>the</strong> one test <strong>fish</strong> floated up dead. GG was not provided with a<br />

copy <strong>of</strong> <strong>the</strong> report.<br />

4/9/2007 – IW on leave – no contact with GG over this period.<br />

IW Diary<br />

24/9/2007<br />

17/09/07 3.30pm 30-40 knots strong variable winds. RA sprays despite<br />

saying <strong>the</strong>re would be no spraying <strong>for</strong> 3 weeks. Supposed to have<br />

GG Diary<br />

entry<br />

covered ponds with tarpaulins, but did not Rang RA- he said he<br />

would take all care<br />

9/10/07 Sprayed without written notice GG Diary<br />

entry<br />

10/10/07 Sprayed without written notice but tarps were on dams <strong>and</strong> block<br />

tanks.<br />

George wanted to spray, so filled rig with water <strong>and</strong> drove along<br />

closest row to fence to show GG how safe it was. GG got<br />

showered in water from <strong>the</strong> rig, st<strong>and</strong>ing on <strong>the</strong> far bank <strong>of</strong> her<br />

pond. George still insisted spraying was safe <strong>and</strong> proceeded to<br />

spray regardless <strong>of</strong> GG’s protest. Smoke fires showed wrong drift<br />

<strong>and</strong> was ignored. Rig was not stopped until all spraying completed<br />

at 4pm. Forced to change entire <strong>fish</strong> farm operations around<br />

GG Diary<br />

entry <strong>and</strong><br />

tape<br />

recording <strong>of</strong> a<br />

discussion <strong>of</strong><br />

this event<br />

(21/10/07)<br />

with Bernie<br />

GG RA <strong>and</strong><br />

Date 28 May 2010 Page 24 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

macadamia operations. RA needed GG & 2 workers to help with George.<br />

<strong>the</strong> labour <strong>for</strong> <strong>the</strong> tarps. As tarps were pulled <strong>of</strong>f it was noted that<br />

<strong>the</strong>y went underwater.<br />

12/10/07 Block tank water killed 2 inch SP fingerlings in hatchery after water<br />

exchange. 1000 sold <strong>the</strong> night be<strong>for</strong>e which were fine still<br />

GG Diary<br />

note<br />

Alleged 4000 SP fingerlings died in hatchery after water change<br />

from block tanks covered by tarpaulins.<br />

14/10/07 The only one GP left as test was found floating in <strong>the</strong> uncovered<br />

dam<br />

GG Diary<br />

note <strong>and</strong><br />

photo<br />

18/10/07 3 females injected 2 good GP GG Diary<br />

note<br />

20/10/07 2 good spawnings Source<br />

unknown<br />

21/10/07 Taped meeting <strong>of</strong> RA, George Bernie <strong>and</strong> GG TAPE<br />

RECORDING<br />

?<br />

22/10/07 Spray note covering 22-28/10/07 <strong>for</strong> supracide, copper <strong>and</strong> Spin,<br />

received date not noted by GG<br />

Note in<br />

possession <strong>of</strong><br />

GG<br />

22/10/07 GP de<strong>for</strong>med <strong>and</strong> spinning GG Diary<br />

note<br />

23/10/07 9.00am GG reports <strong>fish</strong> deaths at <strong>the</strong> hatchery allegedly from IW Report<br />

spraying on <strong>the</strong> Macadamia property stating concerns about <strong>the</strong><br />

use <strong>of</strong> bulldock <strong>and</strong> supracide.<br />

GG alleged tarpaulins erected over <strong>the</strong> ponds <strong>and</strong> block tanks may<br />

have fallen in to <strong>the</strong> ponds <strong>and</strong> tanks, releasing toxic material<br />

which could have killed <strong>the</strong> <strong>fish</strong>. Claimed 4000 silver perch<br />

fingerlings killed after using water from <strong>the</strong> block tanks.<br />

Inspector visited property, GG said that <strong>the</strong>re was spraying on <strong>the</strong><br />

macadamia property <strong>the</strong> previous day <strong>and</strong> rain overnight may have<br />

washed contaminated water into <strong>the</strong> tanks. GG advised that <strong>the</strong><br />

dead <strong>fish</strong> had been discarded.<br />

GG said she was aware that Bulldock, Copper, Spin <strong>and</strong> Supracide<br />

were being used on <strong>the</strong> Macadamia property. GG advised that<br />

smoke fires were being used to check wind strength <strong>and</strong> direction.<br />

G Gallagher (Manager Farm Operations <strong>for</strong> Arbuckle) said smoke<br />

fires were being used to monitor wind conditions <strong>and</strong> he was using<br />

a wind measuring device <strong>and</strong> that spraying was only done when<br />

wind conditions were suitable <strong>and</strong> would not affect <strong>the</strong> hatchery.<br />

Spraying was halted if winds become unsuitable.<br />

23/10/07 IW visits Macadamia, farm spray records inspected <strong>and</strong> chemicals<br />

used on previous day identified. Advised that spraying <strong>of</strong> same<br />

chemicals was continuing on 23/10/2007.<br />

IW Report<br />

Date 28 May 2010 Page 25 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

Copies <strong>of</strong> records provided to IW within two days.<br />

25/10/07 3 GP females <strong>and</strong> 3 SP females induced GG Diary<br />

note<br />

27/10/07 2 good tanks <strong>of</strong> GP now start showing spinning- dump <strong>and</strong> start<br />

again 3 female GP reinject<br />

GG Diary<br />

note<br />

27/10/07 2 nd batch <strong>of</strong> GP breeders spawned. One went 9 hours early <strong>and</strong><br />

had de<strong>for</strong>med embryos. Second female spawned OK, fertilised<br />

eggs OK, but eggs died over 24 hours.<br />

Third female spawned 3 days late- started 95% good developing<br />

GG Diary<br />

note <strong>and</strong><br />

microscope<br />

images<br />

eggs, but 17 hours later progressively died until 30% left. Bent tail<br />

observed.<br />

27/10/07 De<strong>for</strong>med <strong>and</strong> spinning GP GG Diary<br />

note<br />

29/10/07 Water washed from tarp <strong>and</strong> empire gudgeons exposed as an<br />

experiment, <strong>the</strong>y all died.<br />

GG Diary<br />

entry<br />

Owner noted abdominal pain <strong>and</strong> diarrhoea- was unable to feed<br />

her horses. Had just been trying to manually weed <strong>the</strong> ponds.<br />

30/10/07 3 spawnings good eggs good hatch SP. Day 2 after hatch small<br />

round <strong>of</strong> deaths in tanks but rest looked OK. Expected to get<br />

100,000 @ 35mm. Stocked into pond red 2. On 20 Jan 08 @ 11<br />

weeks <strong>of</strong> age fingerlings were harvested. Only 30,000 very weak<br />

very thin larvae were caught. Moved to a new plankton pond <strong>for</strong><br />

more food. None survived, 5 weeks later none found.<br />

GG Photo <strong>of</strong><br />

11 week <strong>of</strong><br />

fingerling<br />

versus a<br />

<strong>health</strong>y 7<br />

week old<br />

atropine<br />

treated<br />

fingerling.<br />

GG Diary<br />

entries.<br />

30/10/07 GG reports to IW de<strong>for</strong>mities in <strong>fish</strong> fry, that some <strong>fish</strong> had been IW Report<br />

killed <strong>and</strong> all <strong>the</strong> brood stock were dead. GG referred to Dr Roger<br />

Chong.<br />

30/10/07 GG contacted by Roger Roger Chong (RC)(Principal Veterinary<br />

Officer DPI&F- Biosecurity Sciences Laboratory) following referral<br />

from IW regarding <strong>fish</strong> <strong>health</strong> issues at <strong>the</strong> hatchery. On <strong>the</strong> basis<br />

<strong>of</strong> <strong>the</strong> history <strong>and</strong> clinical signs <strong>of</strong> spinning <strong>of</strong> <strong>the</strong> fry, which RC<br />

explained was neurological abnormality, GG was advised by RC to<br />

treat any affected <strong>fish</strong> with atropine bathing at 0.1ppm to effect <strong>and</strong><br />

to pre-filter water used inside <strong>the</strong> hatchery shed with activated<br />

carbon at 75g/40L water, refresh carbon every 2 weeks, depending<br />

on efficacy to adsorb pesticide contaminant in water. Atropine was<br />

to be sourced from GG’s local veterinarian. 1 bottle <strong>of</strong> 20ml <strong>of</strong><br />

atropine sulphate was prescribed by Owen Johnstone. RC advised<br />

GG to collect preserve <strong>and</strong> store samples <strong>for</strong> pathology (<strong>for</strong>malin<br />

fixed) <strong>and</strong> <strong>for</strong> pesticides testing (frozen) <strong>and</strong> to await instructions<br />

RC notes<br />

Date 28 May 2010 Page 26 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

<strong>for</strong> collection <strong>of</strong> samples according to legal/DPI&F requirements<br />

30/10/07 Fry photographed. Then treated with atropine. By 2am 31/10/07<br />

improvement was dramatic. About 30-40,000 fry survived <strong>and</strong> were<br />

GG Diary<br />

entry<br />

put out to a plankton pond 2/11/07 Approximately 5-10,000<br />

survived <strong>and</strong> grew at <strong>the</strong> normal rate<br />

31/10/07 Last GP eggs hatched, de<strong>for</strong>med, spinning <strong>and</strong> curly GG Diary<br />

note<br />

31/10/07 Owner diarrhoea GG Diary<br />

entry<br />

31/10/07 Inspector Walker visits hatchery. Is advised by GG she had IW Report<br />

discarded <strong>the</strong> affected fry. GG showed IW fry she claimed had<br />

been treated with atropine in a breeding tank in <strong>the</strong> hatchery.<br />

GG – Gwen Gilson<br />

RA roger Arbuckle<br />

IW – Ian Walker<br />

SP –Silver perch<br />

GP – Golden Perch<br />

Fish Health: The notes in <strong>the</strong> timeline do not accurately describe when <strong>fish</strong> <strong>health</strong> issues<br />

occurred. Four thous<strong>and</strong> silver perch fingerlings died on 12/10/07. One golden perch died on<br />

14/10/07 in a dam. Golden perch (number unstated) de<strong>for</strong>med <strong>and</strong> spinning on 22/10/07.<br />

Two <strong>of</strong> <strong>the</strong> three Golden perch tanks start spinning <strong>and</strong> discarded; late <strong>and</strong> early spawning<br />

in golden perch (after reinjection <strong>of</strong> unstated product) on 27/10/07. Second batch <strong>of</strong> three<br />

golden perch spawn de<strong>for</strong>med <strong>and</strong> spinning on 27/10/07. Three successful silver perch<br />

spawnings on 30/10/07. Fry (species unstated) were treated with atropine on 30/10/07. It is<br />

unclear which batch <strong>of</strong> fry were treated. Golden perch eggs hatch <strong>and</strong> described as<br />

de<strong>for</strong>med spinning <strong>and</strong> curly. Ian Walker indicates that <strong>fish</strong> <strong>health</strong> issues were reported on<br />

23/10/07.<br />

Application <strong>of</strong> pesticides: The spraying according to <strong>the</strong>se notes occurred on 17 September<br />

2007 <strong>and</strong> 9, 10, 23 (possibly 22 but unclear) October 2007. Spray note <strong>for</strong> 22-28/10/07<br />

indicates use <strong>of</strong> Supracide (methidathion), copper <strong>and</strong> Spin (carbendazim).<br />

Samples: Mrs Gilson was advised to collect samples on 30/10/07; however, no samples<br />

were collected. The timeline states that Arbuckle had a water sample taken; however, no<br />

analytical result is known.<br />

Spray records<br />

The following in<strong>for</strong>mation is from a compilation <strong>of</strong> <strong>the</strong> Arbuckle spray records (document<br />

403) prepared by Ian Walker.<br />

Date Block Nos. Chemical Spraying Times/Wea<strong>the</strong>r Conditions<br />

Date 28 May 2010 Page 27 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Block Nos. Chemical Spraying Times/Wea<strong>the</strong>r Conditions<br />

30/07/2007 T1-A2 Carbendazim Start: 3.05 pm (SE 3 – 5 km/hr)<br />

Copper<br />

Oxychloride<br />

Finish: 4.08 pm<br />

T2- A2 Carbendazim<br />

Copper<br />

Oxychloride<br />

Start: 4.55 pm (Wind NE/SE)<br />

Finish A2: 5.44 pm<br />

Start A1, B1<br />

Finish: T2: 6.00 pm<br />

31/07/2007<br />

3/08/2007<br />

T3- A1, B1 Carbendazim<br />

Copper<br />

Oxychloride<br />

T4<br />

Carbendazim<br />

Copper<br />

Oxychloride<br />

T5- A1, B1 Carbendazim<br />

Copper<br />

Oxychloride<br />

Start: 12.22 pm (E/NE occasionally<br />

SE)<br />

1 pm wind less than 1 km/hr<br />

Finish: 1.33 pm<br />

Start: 2 pm (ESE < 2 km/hr)<br />

Finish: 3.15 pm<br />

Start: 8.35 am (S/SE < 1km/hr)<br />

Finish: 9.50 am<br />

(10 am: E/SE occasional NE)<br />

T6- A1, B1 Carbendazim<br />

Copper<br />

Oxychloride<br />

B4<br />

Carbendazim<br />

Copper<br />

Oxychloride<br />

T7- C1 Carbendazim<br />

Copper<br />

Oxychloride<br />

T8, C1 Carbendazim<br />

Copper<br />

Oxychloride<br />

9/10/2007 T1 in B2/B4 Carbendazim<br />

Copper<br />

Oxychloride<br />

T2- B4, C1 Carbendazim<br />

C2<br />

Copper<br />

Oxychloride<br />

T3 – Knob Carbendazim<br />

Copper<br />

Oxychloride<br />

10/10/2007 T4 Carbendazim<br />

Copper<br />

Oxychloride<br />

Start: 10.13 am<br />

Finish: 10.48 am on A1, B1<br />

Start: 10.55 am<br />

Finish: 12 pm (ESE/NE < 5km/hr)<br />

Start: 1.40 pm (NE/N occasional SE<br />

< 1 km/hr)<br />

Finish: 3.00 pm<br />

Start: 3.20 pm<br />

Finish: 4.45 pm<br />

(Wind at 11.53 am N/NW < 1km/hr)<br />

Start: 12 pm<br />

Finish: 1.15 pm (N/NE < 2km/hr)<br />

Finish: T2 at 3.10 pm<br />

Start: 3.26 pm (N/NW occasional NE<br />

< 1km/hr)<br />

Finish: 4.20 pm<br />

Start: 8.55 am (S/SE < 5 km/hr)<br />

Finish: 10.05 am<br />

Date 28 May 2010 Page 28 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Block Nos. Chemical Spraying Times/Wea<strong>the</strong>r Conditions<br />

T5<br />

Carbendazim Start: 1.50 pm (S/SE < 5 km/hr)<br />

Gilson Copper<br />

Oxychloride<br />

Finish: 2.10 pm<br />

T6<br />

Carbendazim Start: 2.34 pm<br />

Copper Finish: Not recorded<br />

Oxychloride<br />

T7 Carbendazim Start: 3.25 pm<br />

Gilson Copper Finish: 3.50pm<br />

Oxychloride<br />

T8 Carbendazim Start: 3:10 pm (SE/E < 2km/hr)<br />

Central Copper Finish: 3.45<br />

Oxychloride<br />

T9<br />

Carbendazim Spraying by R Arbuckle<br />

22/10/2007 T1- A2<br />

Copper<br />

Oxychloride<br />

Carbendazim<br />

Methidathion<br />

Oil<br />

Start: 10.20 am (E/SE < 3 km/hr)<br />

11.35 am (SE/E < 3 km/hr)<br />

T2-A1/B1 Carbendazim<br />

Methidathion<br />

Oil<br />

Start: 12.05 pm<br />

Finish: 1.06 pm (SE/NE


SUMMARY OF RESULTS FOR NFHIT<br />

Date Block Nos. Chemical Spraying Times/Wea<strong>the</strong>r Conditions<br />

Oil<br />

Finish: 4.52 pm (E < 1 km/hr)<br />

T9- B4<br />

T10- Central<br />

Carbendazim<br />

Methidathion<br />

Oil<br />

Carbendazim<br />

Methidathion<br />

Oil<br />

Start: 5.18 pm (E/NE < 1 km/hr)<br />

Start: 1.08 pm (E/SE/NE < 10 km/hr) (largest<br />

nozzles 10 bar, A2 at 1800 rpm)<br />

:2.15 pm (NE top <strong>of</strong> hill < 10 km/hr)<br />

:2.45 pm unchanged<br />

24/10/2007 T2 –Central<br />

House<br />

Carbendazim<br />

Methidathion<br />

Oil<br />

Start: 4.36 pm (E/NE < 5 km/hr)<br />

Finish: 4.56 pm<br />

Start: 5.03 pm<br />

:5.13 pm wind decreases < 3 km/hr)<br />

Finish: 5.50 pm (E/NE < 1 km/hr)<br />

25/10/2007 T13 T2<br />

Start Row<br />

14/15)<br />

Carbendazim<br />

Methidathion<br />

Start: 9.35 am (NE/N < 3 km/hr)<br />

9.40 am (E/NE < 3 km/hr)<br />

Comments: it is unstated how <strong>the</strong> wind speed, direction <strong>and</strong> humidity were measured. The<br />

spray records indicate spraying <strong>of</strong> carbendazim <strong>and</strong> copper oxychloride on 30 <strong>and</strong> 31<br />

September 2007 <strong>and</strong> 3 <strong>and</strong> 9, 10 October 2007 <strong>and</strong> methidathion, oil <strong>and</strong> carbendazim on<br />

<strong>the</strong> 22, 23, 24 <strong>and</strong> 25 October 2007 <strong>and</strong> 1 <strong>and</strong> 5 November 2007. This record conflicts with<br />

<strong>the</strong> Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation (document 300) in<strong>for</strong>mation<br />

on spraying. Last spray application prior to treatment <strong>of</strong> fry with atropine was 5 days.<br />

Samples<br />

nil<br />

February 2008 – Event 3 Syndrome 4 – De<strong>for</strong>mities <strong>and</strong> ill thrift.<br />

From <strong>the</strong> Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation (document 300), <strong>the</strong><br />

following entries occur <strong>for</strong> February 2008:<br />

Fish <strong>health</strong> issues<br />

Date Reported Event Supporting<br />

evidence<br />

February New 30 SP from Gympie produced 2 spawnings <strong>of</strong> 100,000. Water RC notes<br />

2008 from <strong>the</strong> ringtail ponds were carted daily to Gilson Rd property <strong>for</strong><br />

use in <strong>the</strong> hatchery shed. Carbon was used to filter water but<br />

atropine was discontinued because <strong>of</strong> concerns her customers<br />

would not buy medicated <strong>fish</strong>. Spraying reportedly stopped over<br />

this period.<br />

2/2/08 3 female SP breeders from Borumba GG Diary<br />

Date 28 May 2010 Page 30 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

note<br />

3/2/08 SP eggs good GG Diary<br />

note<br />

4/2/08 RA rang re production<br />

Inject 2 female GP from Borumba trip<br />

GG Diary<br />

note<br />

2 GP failed.<br />

SP 95-98% normal eggs stocked out to red 2<br />

15/2/08 New YB <strong>and</strong> 4 SP females from Gympie John <strong>and</strong> Joan’s injected GG Diary<br />

note<br />

22/2/08 Red 4 stocked with SP- clean spawning<br />

GP failed (very late in <strong>the</strong> season to be trying to spawn GP)<br />

GG Diary<br />

note<br />

28/2/08 2 female SP hatched GG Diary<br />

note<br />

Fish Health: Golden perch (presumably 2- spawnings) failed 2 February 2008 <strong>and</strong> an<br />

unstated number on 22 February 2008. Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish Health<br />

Investigation (document 300) states it is very late to be spawning golden perch.<br />

Application <strong>of</strong> pesticides: no in<strong>for</strong>mation<br />

Samples: no in<strong>for</strong>mation<br />

Spray records<br />

No pesticide applications occurred from 5/11/07 till 9/9/2008 as result <strong>of</strong> a change on<br />

management <strong>of</strong> <strong>the</strong> macadamia farm according to <strong>the</strong> Report on Fish Health Incidents,<br />

Sunl<strong>and</strong> Fish Hatchery, 29/4/09 (document 404).<br />

Samples<br />

There is no record <strong>of</strong> any samples taken in relation to this event.<br />

July 2008 – Event 4, Syndrome 3 – De<strong>for</strong>mities.<br />

Fish <strong>health</strong> issues<br />

From <strong>the</strong> timeline (document 300), <strong>the</strong> following entries occur <strong>for</strong> July <strong>and</strong> August 2008:<br />

Date Reported Event Supporting<br />

evidence<br />

July 2008 Collected Noosa River AB: 4 female 3 male. Appeared to RC notes <strong>and</strong><br />

be in good body condition, well muscled <strong>and</strong> normal. GG diary<br />

Spawning 1: Saltwater trucked in <strong>for</strong> hatchery use. entry<br />

16/07/08 Australian Bass caught Lake Cooroibah. IW Report<br />

24/7/2008 Mr Arbuckle advised Inspector Walker that Mr Ziesemer IW Report<br />

Date 28 May 2010 Page 31 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

August<br />

2008<br />

now taking over responsibility <strong>for</strong> growing macadamias on<br />

<strong>the</strong> property.<br />

Spawning 1- Abnormal changes in <strong>the</strong> development <strong>of</strong> <strong>fish</strong><br />

embryos, producing de<strong>for</strong>mities at an estimate <strong>of</strong> 90-99%<br />

<strong>of</strong> batch. The embryonic development was slow at 40 +<br />

hours (normal 27 hours) post chorulon hormone spawning<br />

induction. Cell division <strong>of</strong> fertilised egg was slow <strong>and</strong><br />

dividing cells were <strong>of</strong> uneven size <strong>and</strong> positionally uneven<br />

in <strong>the</strong> egg. The embryo was slow to <strong>for</strong>m over <strong>the</strong> yolk at<br />

35 hours (normal 17 hours). Approximately 700,000 eggs<br />

with abnormalities were dumped by 20 hours post<br />

fertilisation.<br />

Spawnings 2 & 3- Abnormal development to 20 hrs post<br />

fertilisation similar to spawning 1. GG kept <strong>the</strong> batches to<br />

observe until 35-40 hours <strong>and</strong> observed de<strong>for</strong>mities <strong>of</strong><br />

twin heads, twin hearts in <strong>the</strong> affected larvae. In total an<br />

estimated 2.1 million eggs/<strong>fish</strong> embryos were affected.<br />

Noosa AB spawned embryos with two headed <strong>and</strong> o<strong>the</strong>r<br />

de<strong>for</strong>mities photographed <strong>and</strong> videoed.<br />

Samples collected by GG in <strong>for</strong>malin.<br />

No reported chemical spraying through this period.<br />

Dated photos<br />

<strong>and</strong> histopath<br />

from RC, plus<br />

video <strong>and</strong><br />

GG diary<br />

notes<br />

Fish Health: none reported in timeline <strong>for</strong> July however in August (date unstated) de<strong>for</strong>mities<br />

ulphate.<br />

Application <strong>of</strong> pesticides: no in<strong>for</strong>mation<br />

Samples: no residue samples taken<br />

Spray records<br />

No pesticide applications occurred from 5/11/07 till 9 September 2008 as result <strong>of</strong> a change<br />

on management <strong>of</strong> <strong>the</strong> macadamia farm according to <strong>the</strong> Report on Fish Health Incidents,<br />

Sunl<strong>and</strong> Fish Hatchery, 29/4/09 (document 404).<br />

October 2008 – Event 5, Syndrome 3 – Spinning juveniles.<br />

Fish <strong>health</strong> issues<br />

From <strong>the</strong> timeline (document 300), <strong>the</strong> following entries occur <strong>for</strong> October 2008:<br />

Date Reported Event Supporting<br />

evidence<br />

October<br />

2008<br />

Chemical spray recommenced. 1 GP which was from Gilson<br />

Road, but moved to Ringtail, was brought back to <strong>the</strong> Gilson Rd<br />

Hatchery <strong>for</strong> spawning. Produced spinning larvae at about 90% <strong>of</strong><br />

RC notes<br />

Date 28 May 2010 Page 32 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

batch. Had used block tanks water (outside hatchery shed). Used<br />

carbon filtration but ran out <strong>of</strong> atropine to treat all 500,000 fry,<br />

although a treated small sample which responded to atropine- 3<br />

doses <strong>of</strong> 48-72 hours. Loss <strong>of</strong> approx 500,000 fry.<br />

GG reported de<strong>for</strong>mities in chicks <strong>and</strong> eggs fertilised on Gilson Rd<br />

but hatched in an incubator at Cooroy with 2 <strong>of</strong> 8 chicks de<strong>for</strong>med.<br />

De<strong>for</strong>mities described as twisted necks, legs twisted <strong>and</strong> cannot<br />

st<strong>and</strong>. Gilson hatched a second batch <strong>of</strong> 15 eggs at Gilson Rd <strong>and</strong><br />

de<strong>for</strong>mities occurred in 2 hatched chicks but 13 eggs did not<br />

hatch, GG reported one horse with chronic weight loss <strong>and</strong><br />

diarrhoea. Was euthanased by Owen Johnstone <strong>and</strong> necropsy<br />

per<strong>for</strong>med.<br />

2/10/08 Chemical Residue Laboratory reports residues not detected in CRL Report<br />

Lake Cooroibah water sample.<br />

3/10/08 Check red 1- good take <strong>of</strong> Ewin Maddock bass GG Diary<br />

note<br />

4/10/08 Troy video spray in wrong winds. IW called <strong>and</strong> police GG Diary<br />

note <strong>and</strong><br />

video<br />

4/10/2008 No records <strong>of</strong> contact with QPIF on Saturday 4/10/2008. No IW Diary<br />

records <strong>of</strong> contact on Monday 6/10/2008.<br />

Post Mr Ziesemer implements revised buffer zones <strong>for</strong> spraying IW Report<br />

4/10/2008 pesticides on blocks adjoining Gilson 7 Weaver properties<br />

16 tree rows – Weaver property boundary<br />

4 tree rows –North <strong>of</strong> hatchery plus no treatment in rows to East <strong>of</strong><br />

hatchery<br />

I tree row – West <strong>of</strong> Gilson property spray only in winds blowing<br />

away from hatchery<br />

5/10/08 Found heaps GG Diary<br />

note<br />

6/10/08 Spraying video <strong>of</strong> Denise, GG <strong>and</strong> TZ talking denying he was<br />

spraying when video showed spraying taking place<br />

GG Diary<br />

note <strong>and</strong><br />

video<br />

7/10/08 Spraying next to chook pen, winds OK until last half hour- on video GG Diary<br />

note video<br />

15/10/08 Dr Matt Mat<strong>the</strong>w L<strong>and</strong>os (ML) visits farm, collects samples <strong>of</strong><br />

<strong>for</strong>malin fixed de<strong>for</strong>med bass larvae, frozen SP, frozen GP, fixed<br />

GP larvae, chicken eggs <strong>and</strong> dead chick, collects history takes<br />

photos, <strong>and</strong> delivers samples to RC at lab directly<br />

GG Diary<br />

note GG <strong>and</strong><br />

ML<br />

Fish Health: The notes in <strong>the</strong> Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation<br />

(document 300) do not accurately describe when <strong>fish</strong> <strong>health</strong> issues occurred. Some time<br />

Date 28 May 2010 Page 33 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

during October 2008, 90 percent <strong>of</strong> a batch <strong>of</strong> 500,000 golden perch fry were spinning.<br />

De<strong>for</strong>mities are also described in eggs <strong>and</strong> chicks but <strong>the</strong> species is unstated.<br />

Application <strong>of</strong> pesticides: The spraying according to <strong>the</strong>se notes occurred on <strong>the</strong> 4 <strong>and</strong> 7<br />

October 2008.<br />

Samples: In <strong>the</strong> Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation (document 300),<br />

it is unclear if <strong>the</strong> date <strong>for</strong> <strong>the</strong> Lake Cooroibah sample is a sampling or reporting date or why<br />

<strong>the</strong> sample was taken. The Chemical Residue Laboratory Report 6074 (document 119)<br />

indicates that <strong>the</strong> sampling date was in September 2008 <strong>the</strong>re<strong>for</strong>e does not relate this<br />

incident. Samples <strong>of</strong> <strong>for</strong>malin fixed de<strong>for</strong>med bass larvae, frozen silver perch, frozen golden<br />

perch, golden perch larvae, chicken eggs <strong>and</strong> dead chick are collected on <strong>the</strong> 15 October<br />

2008. However, <strong>the</strong>re is no mention <strong>of</strong> <strong>fish</strong> <strong>health</strong> issues in silver perch or bass.<br />

Adverse Experience Report<br />

An Adverse Experience Report (undated) was sent to <strong>the</strong> APVMA by Mat<strong>the</strong>w L<strong>and</strong>os,<br />

23/8/08 (document 602). The reports states, convulsions continued in 2007 <strong>and</strong> now again in<br />

<strong>the</strong> last week (23/10/08).<br />

Videos<br />

File name: Troy Ziesemer poisoning Fish Hatchery 08.wmv<br />

Date/time: Date <strong>and</strong> time stamp indicate 7/10/08 7:46 intermittent filming until 10:12. Also<br />

recorded 6/10/2008 7:42 until 7:47<br />

Video operator: Unstated<br />

Location on property: Unclear from video. Appears to be filming from north <strong>of</strong> her house <strong>and</strong><br />

west <strong>of</strong> <strong>the</strong> chicken coop <strong>and</strong> within 5-10 metres <strong>of</strong> <strong>the</strong> fenceline <strong>of</strong> <strong>the</strong> macadamia block<br />

called ‘Gilson’.<br />

Video <strong>of</strong>: Smoke fires on <strong>the</strong> Gilson Property <strong>and</strong> discussion <strong>of</strong> spraying between three<br />

persons believed to be Mr Try Zeisemer, Gwen Gilson <strong>and</strong> Denise Weaver.<br />

File name: Troy Ziesemer spraying poison.wmv CD containing <strong>the</strong> file Troy Ziesemer<br />

spraying poison.wmv. On <strong>the</strong> CD was written TZ spraying beside plankton pond 7.10.08. CD<br />

case was written TZ spraying beside plankton pond – smoke fire <strong>and</strong> spraying video 7/12/08:<br />

Date/time: Inserted edit text indicates 7/10/08, date <strong>and</strong> time stamp indicate 7/10/08 10:40<br />

intermittent filming until 12:17<br />

Video operator: Unstated<br />

Location on property: Unclear from video. Appears to be filming from north <strong>of</strong> her house <strong>and</strong><br />

west <strong>of</strong> <strong>the</strong> chicken coop <strong>and</strong> within 5-10 metres <strong>of</strong> <strong>the</strong> fenceline <strong>of</strong> <strong>the</strong> macadamia block<br />

called ‘Gilson’.<br />

Video <strong>of</strong>: Smoke fires on <strong>the</strong> Gilson Property.<br />

Spray records<br />

From <strong>the</strong> Chemical Application Records (document 401) spraying by airblast mister occurred<br />

as follows: refer to macadamia block map (document 400) <strong>for</strong> block details.<br />

Date 28 May 2010 Page 34 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Blocks Active Used<br />

4/10/2008 B3, A2, G2, B2, A3, B2, Knob, B4, C1 C3<br />

7/10/2008 Gilson<br />

7/10/2008 Central, A1, B1, C1<br />

9/10/2008 A1, B1, C1, B4, G2, G1<br />

27/10/2008 Central House<br />

27/10/2008 C3, C2, A2, A3, B3<br />

Cu as hydroxide,<br />

Carbendazim , nonyl phenol<br />

ethylene oxide condensate<br />

Beta-cyfluthrin, nonyl phenol<br />

ethylene oxide condensate<br />

28/10/2008 C1, Knob, G2, B4, B2, B3, A2<br />

28/10/2008 Central, A1, B1, C1, B4, G2, Gilson<br />

29/10/2008 C2, GH<br />

29/10/2008 Gilson, G2<br />

30/10/2008 G5<br />

30/10/2008 G6<br />

31/10/2008 G5<br />

31/10/2008 G6<br />

Data taken from Report Sunl<strong>and</strong> Fish Hatchery IW 290409 (document (404), Appendix 18_<br />

Spray Records Herbicide Applications 2008 (document 428)<br />

Date Active Method<br />

15/10/08 glyphosate Boom<br />

20/10/08 glyphosate Boom<br />

21/10/08 glyphosate Boom<br />

22/10/08 glyphosate Boom<br />

30/10/08 glyphosate Boom<br />

Wea<strong>the</strong>r records (Zeisemer)<br />

Date Time Hum Temp Wind Wind Block Comments<br />

Speed<br />

(km/h)<br />

Direction<br />

4/10/2008 7.10am 64.8% 22.7 Very slight SE B3, A2<br />

4/10/2008 8.10am 60.5% 23.2 Nil - B3, A2 Stopped<br />

spraying<br />

because <strong>of</strong><br />

no wind<br />

4/10/2008 9.25am 54.0% 24.3 4.3 W G2 Spraying<br />

commenced<br />

4/10/2008 10.05am 45.5% 25.1 8.2 N G2 Stopped<br />

spraying<br />

because <strong>of</strong><br />

Date 28 May 2010 Page 35 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Time Hum Temp Wind<br />

Speed<br />

(km/h)<br />

Wind<br />

Direction<br />

Block Comments<br />

wind change<br />

4/10/2008 12.45pm 55.9% 27.2 3.8 N/NE A2, B3,<br />

B2<br />

Recommence<br />

spraying<br />

4/10/2008 1.45pm 47.5% 27.2 5.1 N A3, B2, -<br />

Knob<br />

4/10/2008 2.40pm - 29.2 11.2 MAX N B4 Tank filled up<br />

6.9 AV<br />

Knob<br />

4/10/2008 3.50pm 52.4% 25.6 9.9 MAX<br />

3.2 AV<br />

N/NE - Finished<br />

Knob, Start<br />

C3<br />

4/10/2008 4.45pm 53.2% 24.2 9.7 MAX<br />

7.7AV<br />

N/NE B4, C3,<br />

C1<br />

4/10/2008 5.15pm Completed<br />

Days<br />

Spraying<br />

7/10/2008 10.30am 40.0% 25.6 6.1MAX S Gilson Commence<br />

Spraying<br />

7/10/2008 11.27am - 9.7 SE Gilson No Problems<br />

7/10/2008 12.13pm 52.4% 26.4 13.2 SE Central No Problems<br />

7/10/2008 2.40pm 63.6% 23.6 10.5 SE Central Tank filled up<br />

7/10/2008 4.25pm 66.4% 22.2 7 SE/NE A1, B1 Tank filled up<br />

7/10/2008 6.20pm - - - - A1, B1,<br />

C1<br />

Stopped<br />

spraying<br />

because <strong>of</strong><br />

no wind<br />

9/10/2008 8.00am 74.9% 20.9 7.2 NE/SE G2 Commence<br />

Spraying<br />

9/10/2008 12.20pm 62.2% 24.4 6.4 SE/E G2 Tank filled up<br />

9/10/2008 3.40pm - - - - - Finished<br />

Spraying<br />

27/10/2008 1.20pm 50.9% 25.4 12 MAX<br />

4.8 AV<br />

SE/E B2, B3<br />

Central<br />

Commence<br />

Spraying<br />

27/10/2008 2.10pm 54.6% 25.3 8.9 MAX NE B3,<br />

Central<br />

27/10/2008 2.55pm 55.1% 24.8 6.0 MAX NE Central<br />

27/10/2008<br />

9.7 MAX<br />

Central, Tank filled up<br />

3.35pm 55.7% 24.2 7.4 AV NE A2, A3<br />

27/10/2008<br />

4.30pm 52.1% 23.0 7.7 MAX ENE<br />

Central,<br />

A2, A3<br />

27/10/2008<br />

4.55pm 54.8% 22.0 1.3 MAX NNE<br />

Central,<br />

A2, A3<br />

Stopped<br />

spraying<br />

28/10/2008 8.00am 66.4% 23.1 5.2 MAX SSE Gilson, Commence<br />

Date 28 May 2010 Page 36 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Time Hum Temp Wind Wind Block Comments<br />

Speed<br />

(km/h)<br />

Direction<br />

4.3 AV G1, G3 Spraying<br />

28/10/2008 8.15am 57.7% 23.0 NIL NIL Stopped<br />

spraying<br />

28/10/2008 8.20am 57.7% 23.0 7.1 MAX<br />

4.2 AV<br />

SSE Gilson,<br />

G1, G3<br />

Recommence<br />

spraying<br />

28/10/2008 8.29am - - Slight ENE Gilson,<br />

G1, G3<br />

Stopped<br />

spraying<br />

28/10/2008 9.29am - 25.4 9.6 MAX<br />

3.2AV<br />

NE Central,<br />

A2, B3<br />

Recommence<br />

spraying<br />

28/10/2008 10.00am 50.5% 26.2 7.2 MAX NE A1, B1, No Problems<br />

3.5 AV<br />

A2, B3<br />

28/10/2008 11.20am 54.1% 24.9 7.9 MAX NE, SE A1, B1, No Problems<br />

4.2 AV<br />

B2, C1<br />

28/10/2008 1.30pm 55.3% 25.8 7.3 MAX NE A1, B1, No Problems<br />

Knob,<br />

B4, C2,<br />

C1<br />

28/10/2008 3.00pm 52.2% 25.4 5.8 NE B2,<br />

House,<br />

West,<br />

B4, C1<br />

28/10/2008 4.15pm 55.1% 25.6 8.8 MAX SE, NE G2 Finished<br />

3.5 AV<br />

29/10/2008 7.30am 64.5% 23.5 Slight to<br />

4.6<br />

SE G2 Commence<br />

Spraying<br />

29/10/2008 7.46am 56.4% 23.8 3.8 MAX SSW Gilson,<br />

G2<br />

Commence<br />

Gilson Block<br />

29/10/2008 8.05am - - - SSW Gilson,<br />

G2<br />

Stopped<br />

spraying<br />

29/10/2008 8.15am 54.4% - 4.8 MAX<br />

3.6 AV<br />

SSW Gilson Commenced<br />

spraying<br />

29/10/2008 8.30am - - 3.6 SE Gilson<br />

29/10/2008 8.34am - - Nil - Gilson Stopped<br />

spraying<br />

29/10/2008 8.36am - - 4.3 SSE Gilson Recommence<br />

spraying<br />

29/10/2008 9.00am 65.3% 24.6 5.2 MAX SE Gilson No Problems<br />

29/10/2008 9.10am - - - SE Finished<br />

Gilson, no<br />

problems<br />

29/10/2008 9.45am 51.9% 29.0 6.9 MAX<br />

2.9 AV<br />

NNE GH, G2 Stopped,<br />

wind no good<br />

Date 28 May 2010 Page 37 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Time Hum Temp Wind Wind Block Comments<br />

Speed<br />

(km/h)<br />

Direction<br />

30/10/2008 2.00pm 57.8% 27.0 9.5 MAX E, SE G5, G6 Commenced<br />

spraying<br />

30/10/2008 4.20pm 58.9% 26.6 5.3 MAX ESE,<br />

ENE<br />

G5 Stopped<br />

spraying<br />

31/10/2008 7.30am 69.6% 24.5 6.1 MAX E, ENE G5, G6 Commenced<br />

spraying<br />

31/10/2008 8.25am 62.1% 22.8 3.5 MAX N - Stopped<br />

spraying<br />

Comments: it is unstated how <strong>the</strong> wind speed, direction <strong>and</strong> humidity were measured.<br />

Wea<strong>the</strong>r stations<br />

Tewantin is <strong>the</strong> closest wea<strong>the</strong>r station<br />

The following is taken from Appendix 22_Wea<strong>the</strong>r_Tewantin Oct08 (document 435) from<br />

Reports on Fish Health Incidents, Sunl<strong>and</strong> Fish Hatchery, 29/4/09 (document 404)<br />

Day Temp<br />

max ( O C)<br />

Rain 9am dir 9am<br />

speed<br />

(km/h)<br />

3pm dir 3pm<br />

speed<br />

(km/h)<br />

1 23.9 0 NE 22 EN 19<br />

2 23.8 0 NE 15 NE 19<br />

3 25.5 0 NNW 11 NE 30<br />

4 25.4 0 NNW 13 NNE 31<br />

5 26.4 0 N 13 NNE 30<br />

6 31.7 0 NNW 15 N 17<br />

7 28 0 SW 11 ESE 19<br />

8 25.5 0 N 9 NNE 17<br />

9 23.5 0 SSE 19 ESE 13<br />

10 21 0 ENE 17 NE 4<br />

11 20 8.6 SSW 13 SSE 7<br />

12 22 3.8 S 9 ENE 9<br />

13 24 3.4 SE 9 NE 17<br />

14 24 0 NNE 11 NE 19<br />

15 26 0 NNW 9 NE 19<br />

16 22 64.2 SE 20 SE 20<br />

17 23 36.2 SE 19 ESE 19<br />

18 22 0 S 13 SE 15<br />

19 23 0 ESE 15 ESE 13<br />

20 24 0 ESE 9 ENE 15<br />

21 24 0 N 9 NNE 24<br />

Date 28 May 2010 Page 38 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Day Temp<br />

max ( O C)<br />

Rain 9am dir 9am<br />

speed<br />

(km/h)<br />

3pm dir 3pm<br />

speed<br />

(km/h)<br />

22 24 0 SSE 7 NE 15<br />

23 24 0 S 17 NE 13<br />

24 22 0 S 15 ESE 19<br />

25 23 0 NNE 7 NE 19<br />

26 24 0 SE 6 NE 15<br />

27 23 0 NE 11 NE 17<br />

28 24 0 ENE 11 NE 19<br />

29 25 0 SE 7 E 19<br />

30 25 2.4 EN 15 E 11<br />

31 25 0 NNW 11 NE 22<br />

Samples<br />

Sample 1<br />

BSL sample submission <strong>for</strong>m (Document 202)<br />

Accession No: 08-178975.<br />

Submitter: Mat<strong>the</strong>w L<strong>and</strong>os<br />

Property: Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: unstated<br />

Date received: 17/10/08.<br />

Animal: Fish (Yellowbelly, Silver Perch <strong>and</strong> Bass) <strong>and</strong> chickens<br />

History <strong>and</strong> clinical signs reported: Spinning larvae (Yellowbelly). De<strong>for</strong>med larvae (Bass).<br />

Atropine responsive in Yellowbelly. Correlated with spraydrift <strong>of</strong> Supracide + copper +<br />

carbendazim.<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

unstated<br />

unstated<br />

Yellowbelly eggs – spinning<br />

larvae<br />

Silverperch survivors after<br />

outside water used<br />

frozen<br />

unstated<br />

OP/OC/SP<br />

OP/OC/SP<br />

Comments: It is unstated what containers were used, how all <strong>the</strong> samples were stored. No<br />

in<strong>for</strong>mation included on sampling methodology. Laboratory numbers not stated. Lab<br />

numbers DP1893 <strong>and</strong> DP1894 mentioned. Sampling date not stated. The agricultural<br />

chemicals identified in <strong>the</strong> document above are in conflict with <strong>the</strong> chemical application<br />

records.<br />

Chemical Residue Laboratory Report 6134 (Documents 105, 117)<br />

Date 28 May 2010 Page 39 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Submitter: Roger Chong Date sampled: 16/10/08 Method: AP016<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical Result<br />

(mg/kg)<br />

Ref link Submission<br />

<strong>for</strong>m<br />

DP1893 Small silver perch DDT (total) 0.02 #08-178975 202<br />

Multi residue<br />

(o<strong>the</strong>rs)<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Data taken from Appendix 18_ Spray Records Herbicide Applications 2008 (document 428)<br />

from Report Sunl<strong>and</strong> Fish Hatchery IW 290409 (document (404)<br />

Date/time Block/Row<br />

3/11/08 G1,G2,G3,G4,G5,G6,<br />

GH<br />

Fertiliser<br />

Calcined<br />

magnesium<br />

4/11/08 The rest Calcined<br />

magnesium<br />

27/11/08 ???? Incitec<br />

93549<br />

Samples<br />

Sample 1<br />

BSL sample submission <strong>for</strong>m (Document 200)<br />

Accession No: 08-180371.<br />

Submitter: Mat<strong>the</strong>w L<strong>and</strong>os<br />

Property: FNS0292 Sunl<strong>and</strong> Fish Hatchery.<br />

Date sampled: unstated<br />

Date received: 4/11/08.<br />

Animal: 25 year old warm blood mare.<br />

History <strong>and</strong> clinical signs reported: Scouring on <strong>and</strong> <strong>of</strong>f <strong>for</strong> 3-4 years. Necropsied by O<br />

Johnston. Mare goes into pond <strong>and</strong> eats water lilies. Property owner concerned about<br />

spraydrift.<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1900<br />

DP1901<br />

DP1902<br />

1 x small intestine contents Frozen Pesticides<br />

1 x stomach contents Frozen Pesticides<br />

2 x kidney Frozen Pesticides<br />

2 x liver Frozen Pesticides<br />

1 x muscle fat Frozen Pesticides<br />

1 x kidney fat Frozen Pesticides<br />

Attachment to BSL sample submission <strong>for</strong>m:<br />

IDEXX laboratories sample submission <strong>for</strong>m. Submitted by Owen Johnston on an unstated<br />

date <strong>for</strong> property QFNS0292.<br />

Chemical Residue Laboratory Report 6148 (Document 116)<br />

Submitter: Mat<strong>the</strong>w L<strong>and</strong>os Date sampled: 1/11/08<br />

Method: AP016<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical<br />

Result<br />

(mg/kg)<br />

Ref link<br />

Submission<br />

<strong>for</strong>m<br />

Date 28 May 2010 Page 41 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

DP1900<br />

DP1901<br />

Sample<br />

descriptor<br />

Pooled stomach<br />

content/ small<br />

intestine<br />

Pooled liver/<br />

kidney<br />

Chemical Result<br />

(mg/kg)<br />

Ref link Submission<br />

<strong>for</strong>m<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

water only. Left hatchery shed roller door open on several<br />

occasions <strong>and</strong> spawning tanks were uncovered. About 10<br />

hours post hatch <strong>the</strong>se fry died. Chemical sprays were<br />

reportedly occurring- no spray notices provided.<br />

1/12/08 Spray winds OK GG Diary<br />

note<br />

3/12/08 Inject 3 GP females, spraying in wrong winds on video<br />

reported to DPIF<br />

GG Diary <strong>and</strong><br />

video<br />

3/12/08 GG advised IW spraying <strong>of</strong> Macadamias was causing IW Report<br />

spray drift onto <strong>the</strong> hatchery <strong>and</strong> that <strong>the</strong> wind was fairly<br />

strong.<br />

3/12/2008 Inspector Walker <strong>and</strong> Inspector Shields visit Macadamia IW Report<br />

Orchard <strong>the</strong>y observed spraying from 2.30pm. Advised by<br />

Mr Ziesemer that Bulldock was being sprayed. A smoke<br />

fire observed on <strong>the</strong> Nor<strong>the</strong>rn side <strong>of</strong> <strong>the</strong> dam. Observed<br />

spray operator taking <strong>and</strong> recording wind measurements.<br />

4/12/08 IW called re wrong winds <strong>and</strong> spraying. Told GG he can’t<br />

act.<br />

GG Diary<br />

note<br />

4/12/08 2.30pm GG dem<strong>and</strong>ed IW visit <strong>the</strong> Macadamia Farm <strong>and</strong> IW Report<br />

stop <strong>the</strong> spraying.<br />

5/12/08 98% gd eggs GP (sprayed winds OK) GG Diary<br />

note<br />

6/12/08 All GP fry died not known why Possibly<br />

some histo<br />

now done on<br />

<strong>the</strong>se<br />

7/12/08 Dead SP in ‘males’ pond 6 dead video <strong>fish</strong> lying in<br />

shallows<br />

GG Diary<br />

note video<br />

<strong>and</strong> photo<br />

7/12/08 GG left a message on Inspector Walker’s mobile phone IW Report<br />

reporting <strong>the</strong> death <strong>of</strong> six silver perch in one pond <strong>and</strong> that<br />

several o<strong>the</strong>r <strong>fish</strong> were breaking surface <strong>of</strong> <strong>the</strong> water <strong>and</strong><br />

swimming on <strong>the</strong>ir sides.<br />

7/12/08 Males pond with dead SP about 12 x 1kg size <strong>fish</strong>. RC notes<br />

Chemical spray reportedly occurring- no spray notices<br />

provided. Spray rig reported started spraying on 4/12/08 in<br />

<strong>the</strong> first two rows <strong>of</strong> macadamia trees adjacent to <strong>the</strong><br />

males pond, when this was supposedly a “buffer zone”<br />

indicated by TZ to RA that it would not be sprayed.<br />

2 nd lot <strong>of</strong> GP spawners, just sourced, <strong>and</strong> stocked 8/12/08<br />

7/12/08 reported <strong>fish</strong> kill <strong>of</strong> 6 SP <strong>and</strong> alleged several o<strong>the</strong>r<br />

<strong>fish</strong> were breaking <strong>the</strong> surface <strong>and</strong> swimming on <strong>the</strong>ir<br />

sides.<br />

8/12/08 IW visited <strong>the</strong> hatchery at approx 10.30am. GG showed IW Report<br />

Date 28 May 2010 Page 43 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

<strong>the</strong> inspector <strong>the</strong> males pond <strong>and</strong> claimed five silver perch<br />

had died <strong>and</strong> eight more were showing signs <strong>of</strong> distress.<br />

GG said <strong>the</strong> <strong>fish</strong> deaths were noticed at 7.30pm on<br />

7/12/2008. IW observed three dead <strong>fish</strong> on <strong>the</strong> pond bank<br />

<strong>and</strong> one dead <strong>fish</strong> in <strong>the</strong> pond. No sign <strong>of</strong> <strong>fish</strong> swimming<br />

abnormally at <strong>the</strong> time.<br />

RC Notes<br />

Digital<br />

recording<br />

Sample<br />

Water samples were obtained from <strong>the</strong> Males pond, <strong>the</strong><br />

barra pond <strong>and</strong> a spawning tank inside <strong>the</strong> hatchery. GG<br />

advised <strong>the</strong>re were only ten <strong>fish</strong> in <strong>the</strong> barra pond, no<br />

dead <strong>fish</strong> observed.<br />

submission<br />

<strong>for</strong>ms<br />

GG provides digital video recording <strong>of</strong> smoke fire to <strong>the</strong><br />

inspector.<br />

8/12/08 6 more SP mortalities floated GG diary<br />

notes<br />

9/12/08 RC <strong>and</strong> Max Wingfield visit farm, collect baby SP from red GG diary<br />

7 <strong>and</strong> 1 moribund SP from ‘males’ pond. Then spent half a note<br />

day in pond pulling out lilies so more sick SP could be<br />

caught easily.<br />

9/12/08 First pre-arranged visit <strong>of</strong> RC (accompanied by Max RC notes<br />

Wingfield- Fisheries Biologist) to conduct a veterinary <strong>fish</strong><br />

<strong>health</strong> investigation, following authorisation from <strong>the</strong><br />

Director <strong>of</strong> Biosecurity Sciences- Jim Thompson on <strong>the</strong><br />

5/12/08. Sample s<strong>of</strong> water <strong>and</strong> SP <strong>fish</strong> were collected<br />

from Gilson Rd <strong>and</strong> <strong>the</strong> Ringtail property <strong>for</strong><br />

histopathology, pesticides residue, metals residue,<br />

nodavirus PCR, brain/tissue acetylcholinesterase (AchE)<br />

testing accession nos 08-182660(ulpha Rd) <strong>and</strong> 08-<br />

179890 (ringtail) samples. Samples were collected in<br />

accordance with <strong>the</strong> Sunl<strong>and</strong> Fish Hatchery Veterinary<br />

Investigation Work Instruction Version 4/4, 6.12.08<br />

10/12/08 22 dead SP ‘males pond’ video GG Diary<br />

note<br />

12/12/08 ~40 dead SP in males pond <strong>and</strong> mullet in same pond die GG Diary<br />

note<br />

12/12/08 GG reported that 40 SP <strong>and</strong> 1 mullet found dead in <strong>the</strong><br />

males pond cumulatively since 7/12/08<br />

13/12/08 2 dead SP males pond GG Diary<br />

note<br />

14/12/08 3 dead SP males pond GG Diary<br />

note<br />

15/12/08 Inject 2 SP females (Bryce’s) GG Diary<br />

note<br />

Date 28 May 2010 Page 44 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event Supporting<br />

evidence<br />

15/12/08 Hatchery advised that <strong>fish</strong> have progressively died up until IW Report<br />

14/12/08 in <strong>the</strong> males pond with a total number <strong>of</strong> deaths<br />

at 45. No <strong>fish</strong> reported to have died in <strong>the</strong> barra pond<br />

16/12/08 RC visit wanted <strong>health</strong>y <strong>fish</strong> as “normals” sampled Bryces GG Diary<br />

note<br />

18/12/08 Embryo de<strong>for</strong>med <strong>and</strong> dying photos <strong>and</strong> video GG Diary<br />

note<br />

20/12/08 Weeded rest <strong>of</strong> males pond GG Diary<br />

note<br />

21/12/08 5 dead SP GG Diary<br />

note<br />

22/12/08 7 dead SP 1 dead GP 1 dead mullet GG Diary<br />

note<br />

14/01/09 Chemical Residue Laboratory reports. No residues CRL Report<br />

detected in <strong>the</strong> three water samples taken from <strong>the</strong><br />

hatchery by IW on 8/12/08<br />

23/01/09 Qld Health water test- no security ties used on sample<br />

bottles<br />

GG Diary<br />

note<br />

Fish Health: Golden perch fry die (number unstated) on 6/12/08. Six silver perch dead in<br />

‘Males Pond’ on 7/12/08. The Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation<br />

(document 300) also indicates that 12 x 1 kg silver perch died in ‘Males Pond’ on <strong>the</strong> same<br />

date. Six more silver perch died on 8/12/08. Ten silver perch dead in males pond on<br />

10/12/08. Forty silver perch die in ‘males pond’ on 12/12/08. This is a cumulative total. Two<br />

<strong>and</strong> three more silver perch died on 13 <strong>and</strong> 14/12/08 respectively (total dead unclear.<br />

Numbers <strong>of</strong> dead SP from Gwen Gilson are in conflict with <strong>the</strong> report by Ian Walker). Five<br />

silver perch died on 21/12/08. Seven silver perch, one golden perch <strong>and</strong> one mullet died on<br />

22/12/08.<br />

Application <strong>of</strong> pesticides: spraying occurred on 3, 4, 5/12/08.<br />

Samples: Water <strong>and</strong> silver perch were collected from Gilson Rd <strong>and</strong> <strong>the</strong> Ringtail property <strong>for</strong><br />

histopathology, pesticides residue, metals residue, nodavirus PCR, brain/tissue<br />

acetylcholinesterase (AchE) testing accession no 08-182660 (Gilson Rd) <strong>and</strong> 08-179890<br />

(ringtail) samples. Samples were collected in accordance with <strong>the</strong> Sunl<strong>and</strong> Fish Hatchery<br />

Veterinary Investigation Work Instruction Version 4/4, 6.12.08<br />

Videos<br />

File name: MOV011.MOD, On <strong>the</strong> CD was written spraying rows 1 & 2 3.12.08. On <strong>the</strong> Cd<br />

case was written spraying rows 1 <strong>and</strong> 2 3/12/08<br />

Video operator: Unstated<br />

Date: No date or time stamp on video. CD case indicates 1, 2 <strong>and</strong> 3/12/08<br />

Location on property: Unclear from video.<br />

Date 28 May 2010 Page 45 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Video <strong>of</strong>: Spraying <strong>of</strong> macadamias using a trailer mounted airblast sprayer.<br />

Wea<strong>the</strong>r stations<br />

Tewantin is <strong>the</strong> closest wea<strong>the</strong>r station.<br />

The following is derived from Appendix 22_Wea<strong>the</strong>r_Tewantin Dec08 (document 437) from<br />

Reports on Fish Health incidents, Sunl<strong>and</strong> Fish Hatchery, 29/4/09 (document 404)<br />

Day Temp<br />

max ( O C)<br />

Rain 9am dir 9am<br />

speed<br />

(km/h)<br />

3pm dir 3pm<br />

speed<br />

(km/h)<br />

1 27.6 0 N 11 NNE 20<br />

2 30.5 0 NNW 15 NE 28<br />

3 28.1 0 N 15 NNE 33<br />

4 29.7 0 N 19 NE 22<br />

5 28.0 6.2 NNE 11 NE 22<br />

6 34.2 2.8 NW 9 NE 20<br />

7 32.5 10.2 NNW 9 NW 13<br />

8 26.3 2.20 S 15 S 15<br />

9 27.4 0.60 SSE 7 ENE 17<br />

10 27.8 0 N 11 NE 22<br />

11 30.6 0 N 19 NNE 30<br />

12 29.9 0 NE 6 NE 20<br />

13 35.7 0 NNW 17 NW 11<br />

14 35.4 0.4 W 11 W 15<br />

15 32.1 0 SW 9 W 11<br />

16 27.5 0 E 11 ENE 19<br />

17 27.4 0 N 13 NE 22<br />

18 28.1 0 NNE 11 NNE 28<br />

19 33.4 7.8 N 15 NNE 26<br />

20 26.9 0 SSE 22 SE 24<br />

21 25.4 0.8 SSE 9 SE 20<br />

22 26.1 1.0 SSE 17 SE 17<br />

23 26.9 0.2 NNE 13 NE 20<br />

24 27.4 0 NNW 11 NE 33<br />

25 28.8 0.4 NNW 13 NE 19<br />

26 28.9 1.0 NE 13 NE 19<br />

27 28 0 ENE 17 NE 20<br />

28 28.2 0.4 S 6 NNE 11<br />

29 29.0 0 NNE 11 NNE 19<br />

30 34.4 3.0 N 15 NNE 24<br />

31 35.2 0 SSW 11 NE 17<br />

Date 28 May 2010 Page 46 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Spray records<br />

From <strong>the</strong> attachment 16 to Report on Fish Health Incidents Sunl<strong>and</strong> Fish Hatchery, 29/4/09<br />

(document 426) spraying occurred as follows: refer to macadamia block map (document<br />

400) <strong>for</strong> block details.<br />

Date Blocks Active Used<br />

1/12/2008 A1, B1<br />

3/12/2008 Central Knob, A1, B1, C1, C2, C3<br />

4/12/2008 C1, Knob, A2, A3, B2, B3, G5, G6, GH,<br />

G1<br />

Beta-cyfluthrin, Nonylphenol<br />

ethoxylate condensate, zinc<br />

5/12/2008 G5, G2<br />

8/12/2008 Gilson, G1, G2, G3, G4<br />

9/12/2008 G1<br />

Data taken from Appendix 18_ Spray Records Herbicide Applications 2008 (document 428)<br />

to Report Sunl<strong>and</strong> Fish Hatchery IW 290409 (document (404)<br />

Date Active Method<br />

12/12/08 Glyphosate Boom<br />

15/12/08 Glyphosate Boom<br />

16/12/08 Glyphosate Boom<br />

17/12/08 Glyphosate Boom<br />

Comments: Dates <strong>of</strong> spraying are in conflict with <strong>the</strong> Timeline <strong>of</strong> Events <strong>for</strong> <strong>the</strong> Noosa Fish<br />

Health Investigation (document 300).<br />

Wea<strong>the</strong>r Records (Zeisemer)<br />

Date Time Hum<br />

(%)<br />

Tem<br />

p<br />

( O C)<br />

Wind<br />

Speed<br />

(km/h)<br />

Wind<br />

Dir<br />

Block<br />

2/12/2008 7.00am 75.2 22.6 5.3 max SE G1, G3,<br />

G4<br />

8.10am 64.1 23.0 7.7 max SE G1, G3,<br />

G4<br />

9.30am 65.7 23.0 7.1 max SE, G1, G3,<br />

SW G4<br />

11.00am 60.9 25.2 7.4 max SE G1, G3,<br />

G4<br />

12.45pm 56.9 28.1 5.4 max SE G1, G3,<br />

1.9 av<br />

G4<br />

Comments<br />

Commence<br />

spraying<br />

No problems<br />

Date 28 May 2010 Page 47 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

1.26pm 54.3 27.7 7.7 max E, SE G1, G3, Blocks finished<br />

G4<br />

4.10pm 50.2 27.8 9.0 max SE,<br />

NE<br />

B1, A1 Commenced<br />

spraying<br />

5.50pm 68.7 25.3 1.3 max N, NE B1, A1 Stopped<br />

spraying<br />

3/12/2008 10.00am 53.4 29.9 7.1 max<br />

5.7 min<br />

Due<br />

N<br />

B1, A1,<br />

Knob,<br />

Commence<br />

Spraying<br />

C2, C3<br />

11.15am 54.5 29.4 3.8 max<br />

3.1 min<br />

NW B1, A1,<br />

Knob,<br />

Stopped<br />

spraying<br />

C2, C3<br />

12.30am 57.8 29.5 13.1<br />

max<br />

E Central Stopped<br />

spraying<br />

6.0 av<br />

12.45pm 61.9 29.2 11.0 E, NE Central OK<br />

max<br />

2.9 min<br />

1.30pm 58.2 29.8 8.5 max E, NE Central,<br />

C2<br />

Commenced<br />

after refill all OK<br />

3.30pm 49.5 33.2 4.6 min NE Central Tank filled up<br />

5.00pm 50.6 30.5 5.2 max NE Central, OK<br />

C2<br />

5.45pm 54.9 29.6 11.1<br />

max<br />

NE C1, A1,<br />

B1<br />

Tank filled up<br />

7.05pm 77.0 23.7 NIL - - Finished<br />

Spraying<br />

4/12/2008 7.35am 68.2 27.4 5.2 max<br />

3.5 av<br />

NNE Knob, A3 Commenced<br />

spraying<br />

9.45am 61.0 29.5 10.2 N, NE A2, A3, Tank filled up<br />

max 3.5<br />

av<br />

B2, B3<br />

11.10am 47.5 33.2 8.6 max N, G6, G4 Change blocks<br />

3.2 av NW<br />

1.45pm 54.2 30.4 6.2 max<br />

2.2 min<br />

E, SE G5, G4 All good<br />

3.30pm - - 3.2 max<br />

0 min<br />

E G5, G1 Stopped due to<br />

storm<br />

5/12/2008 1.30pm 67.9 27.9 5.5 max<br />

1.3 min<br />

SE G5, G2 Commence<br />

Spraying<br />

5/12/2008 3.00pm - - 7.6 max<br />

2.7 min<br />

N G2 Stopped<br />

spraying<br />

8/12/2008 10.30am 68.8 26.8 6.2 max SE G2, G1,<br />

G4<br />

Commenced<br />

spraying<br />

Date 28 May 2010 Page 48 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

8/12/2008 11.55am 65.5 25.6 11.2 S, SE G1, G3, All OK<br />

max 5.5<br />

av<br />

G4<br />

8/12/2008 1.35pm 66.4 25.9 10.4 S, E Gilson, All OK<br />

%<br />

max<br />

G1, G4<br />

8/12/2008 2.25pm 72.8 24.2 5.9 max SE Gilson, All OK<br />

G1, G4<br />

8/12/2008 4.00pm 73.6 26.8 7.1 max SE G1, G3 All OK<br />

8/12/2008 5.45pm 64.7 25.1 3.3 max SE - Finished blocks<br />

9/12/2008 7.30am 71.8 23.6 3.4 max SE G1 Commenced<br />

spraying<br />

9/12/2008 8.00am - - 3.6 max SE - Spraying<br />

finished<br />

Samples<br />

Sample 1<br />

BSL sample submission <strong>for</strong>m. (Document 206)<br />

Accession No: 09-103042.<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: 8/12/08<br />

Date received: 5/2/09<br />

Animal: adult silver perch<br />

History <strong>and</strong> clinical signs reported: Frozen silver perch collected 8/12/08 from Males pond.<br />

Frozen on same day.<br />

Analysis requested: Pesticides <strong>and</strong> mycology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1926 Gill Frozen OC, OP, SP, cyfluthrin<br />

DP1927 Liver Frozen OC, OP, SP, cyfluthrin<br />

DP1928 Fat Frozen OC, OP, SP, cyfluthrin<br />

DP1929 Gonad Frozen OC, OP, SP, cyfluthrin<br />

DP1930 Sediment (west side) unstated OC, OP, SP, cyfluthrin<br />

DP1931<br />

Sediment (orth, east & south<br />

side)<br />

unstated<br />

Chemical Residue Laboratory Report 6254 (Document 102)<br />

Submitter: Roger Chong Date sampled: 4/2/09 Method: AP016<br />

OC, OP, SP, cyfluthrin<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical<br />

Result<br />

(mg/kg)<br />

Ref link<br />

Submission<br />

<strong>for</strong>m<br />

Date 28 May 2010 Page 49 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

DP1930<br />

DP1931<br />

Sample<br />

descriptor<br />

Sediment (west<br />

side males pond)<br />

Sediment (orth,<br />

east <strong>and</strong> south<br />

side male’s pond)<br />

Chemical Result<br />

(mg/kg)<br />

Ref link Submission<br />

<strong>for</strong>m<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Property: Gwen Gilson (Ring Tail ponds)<br />

Date sampled: 9/12/08<br />

Date received: 10/12/08.<br />

Animal: Silver perch<br />

History <strong>and</strong> clinical signs reported: normal silver perch from hatchery at Illouri place, Ring<br />

Tail.<br />

Analysis requested: Pesticides, metals, virology, biochemistry <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

unstated Fish x 40-50 unstated OP/OC/SP<br />

unstated Fish x 30-40 unstated metals<br />

Unstated Water 2L unstated OP/OC/SP/carbendazim<br />

Unstated Water 250ml unstated metals<br />

Unstated Water 250ml unstated metals (Hg)<br />

Chemical Residue Laboratory Report 6200 (Document 111)<br />

(Al,Zn,Cu,Fe,Cd,Pb,Cr,As)<br />

Submitter: Roger Chong Date sampled: 9/12/08 Method: AP016<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical Result (mg/kg) Ref link<br />

DP1908 Perch fingerlings Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

DP1907<br />

Sample<br />

descriptor<br />

Ring Tail pond<br />

water<br />

Chemical Result Ref link Submission<br />

(mg/kg)<br />

<strong>for</strong>m<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Magnesium 1800<br />

Manganese 10<br />

Mercury


SUMMARY OF RESULTS FOR NFHIT<br />

Date received: 10/12/08<br />

Animal: Silver perch<br />

History <strong>and</strong> clinical signs reported: Fish kill 2 days ago (7/12 1 st dead <strong>fish</strong>). 12 dead total<br />

near 1 kg size silver perch pond males. Spraying <strong>of</strong> macadamias occurring??? (Overwritten<br />

by stamp)<br />

Analysis requested: Pesticides, biochemistry, bacteriology, virology <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1910 Liver/fat Unstated OC, OP, SP, carbendazim<br />

DP1911 Gill/tissue Unstated OC, OP, SP, carbendazim<br />

DP1912 ovary Unstated OC, OP, SP, carbendazim<br />

Unstated Water 2L Unstated Pesticides/ carbendazim<br />

Unstated Water 250 ml Unstated Metals<br />

Unstated Water 250 ml Unstated Metals (Hg)<br />

Chemical Residue Laboratory Report 6201 (Document 114)<br />

Submitter: Roger Chong Date sampled 9/12/08 Method AP016<br />

(Al,Zn,Cu,Fe,Cd,Pb,Cr,As)<br />

Lab No<br />

DP1910<br />

DP1911<br />

DP1912<br />

Sample<br />

descriptor<br />

Fish liver/ fat<br />

Fish gill/ tissue<br />

Fish ovary<br />

Chemical Result<br />

(mg/kg)<br />

DDE 0.09<br />

DDD 0.03<br />

DDT 0.03<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Methidathion 0.05 Trichlorfon 0.1<br />

Cyfluthrin 0.05 Cypermethrin 0.05<br />

p,p’ DDT 0.01 p,p’ DDE 0.01<br />

p,p’ DDD 0.01 Carbendazim 0.1<br />

Alpha endosulfan 0.02 Beta endosulfan 0.02<br />

Endosulfan sulfate 0.02 Chlorpyrifos 0.02<br />

Methoxyfenozide Not tested Fipronil 0.10<br />

Chemical Residue Laboratory Report 6199 (Document 115)<br />

Submitter: Roger Chong Date sampled: 9/12/08 Method: AP016<br />

Lab No<br />

DP1907<br />

Sample<br />

descriptor<br />

Pond M (males)<br />

water<br />

Chemical Result Ref link Submission<br />

(mg/kg)<br />

<strong>for</strong>m<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Date sampled: 10/12/08<br />

Method: 18196<br />

Submitter: Roger Chong<br />

Matrix: water<br />

Analyte<br />

Result (ug/L)<br />

Aluminium 110<br />

Antimony


SUMMARY OF RESULTS FOR NFHIT<br />

Silver perch/gill Silver perch/liver<br />

Analyte Result (mg/kg) Result (mg/kg)<br />

Cadmium


SUMMARY OF RESULTS FOR NFHIT<br />

Lab Ref Description Condition/type Analysis Req<br />

unstated Liver from 3 <strong>fish</strong> Unstated OC, OP, SP, carbendazim<br />

unstated Gonad from 3 <strong>fish</strong> Unstated OC, OP, SP, carbendazim<br />

Form mentions DP1914-DP1916.<br />

Chemical Residue Laboratory Report 6202 (Document 113)<br />

Submitter: Roger Chong Date sampled 16/12/08 Method AP016<br />

Lab No<br />

Sample<br />

Chemical<br />

Result<br />

Ref link<br />

Submission<br />

descriptor<br />

(mg/kg)<br />

<strong>for</strong>m<br />

DP1914<br />

Pooled gills<br />

DDE 0.05<br />

#08-184980 208<br />

DDD 0.01<br />

DDT 0.01<br />

Multi residue<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Comments: It is not stated what containers were used or how all <strong>the</strong> samples were stored.<br />

No in<strong>for</strong>mation included on sampling methodology.<br />

Chemical Residue Laboratory Report 6252 (Document 100)<br />

Submitter: Roger Chong Date sampled DP1914R-DP1916R – 10/12/08, DP1918R –<br />

22/12/08 Method AP016<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical<br />

Result<br />

(mg/kg)<br />

Ref link<br />

DP1914R Pooled gills cyfluthrin


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

Sample<br />

descriptor<br />

(Normal silver<br />

perch)<br />

Chemical<br />

Result<br />

(mg/kg)<br />

Ref link<br />

Submission<br />

<strong>for</strong>m<br />

LOR<br />

Samples were analysed <strong>for</strong> a large number <strong>of</strong> Ops, Ocs, SPs, fungicides <strong>and</strong> insecticides<br />

<strong>and</strong> o<strong>the</strong>r chemicals. Reported on ‘as received’ basis. LORs generally in <strong>the</strong> range: SPs<br />

0.05, Ocs 0.01-0.02, fungicides 0.1-0.2, Ops 0.02-0.1, o<strong>the</strong>r chemicals 0.1-0.2 mg/kg<br />

LORs <strong>of</strong> interest<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Methidathion 0.05 Trichlorfon 0.1<br />

Cyfluthrin 0.05 Cypermethrin 0.05<br />

p,p’ DDT 0.01 p,p’ DDE 0.01<br />

p,p’ DDD 0.01 Carbendazim Not tested<br />

Alpha endosulfan 0.02 Beta endosulfan 0.02<br />

Endosulfan sulfate 0.02 Chlorpyrifos 0.02<br />

Methoxyfenozide Not tested Fipronil 0.10<br />

Comments: It is unstated what containers were used, how all <strong>the</strong> samples were stored. No<br />

in<strong>for</strong>mation included on sampling methodology.<br />

Sample 6<br />

Record <strong>of</strong> Sample Taken FDU0560. (Document 212)<br />

Sample No: 08.0739<br />

Seal no: A02670<br />

Submitter: Ian Walker<br />

Property: G Gilson, Illoura Pl, Gilson Rd<br />

Date sampled: 8/12/08<br />

Date received: 8/12/08<br />

Sample: Water<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

CUC1086<br />

Water from <strong>the</strong> tank inside shed<br />

on western end (spawning shed<br />

closed) Water from <strong>the</strong> Ring Tail<br />

creek property (Illoura Place<br />

property)<br />

unstated<br />

Chemical Residue Laboratory Report 6197 (Document 106)<br />

pesticides<br />

Submitter: Ian Walker Date sampled: 8/12/08 Method: based on EPA 507/508<br />

Date 28 May 2010 Page 60 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

CUC1086<br />

Sample<br />

descriptor<br />

Spawning shed<br />

water<br />

Chemical Result Ref link Submission<br />

(mg/kg)<br />

<strong>for</strong>m<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Chemical Residue Laboratory Report 6196 (Document 107)<br />

Submitter: Ian Walker Date sampled: 8/12/08 Method: based on EPA 507/508<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical Result<br />

(mg/kg)<br />

Ref link Submission<br />

<strong>for</strong>m<br />

CUC1085 Barra pond water Multi residue 1L<br />

unstated<br />

pesticides<br />

Date 28 May 2010 Page 62 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Chemical Residue Laboratory Report 6196 (Document 108)<br />

Submitter: Ian Walker Date sampled: 8/12/08 Method: based on EPA 507/508<br />

Lab No<br />

Sample<br />

Chemical Result Ref link Submission<br />

descriptor<br />

(mg/kg)<br />

<strong>for</strong>m<br />

CUC1084 Males pond water Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1918<br />

Whole brain with fat <strong>of</strong> skull<br />

cavity<br />

Chilled<br />

OC, OP, SP, Carbendazim<br />

Chemical Residue Laboratory Report 6204 (Document 123)<br />

Submitter: Roger Chong Date sampled: 22/12/08 Method: AP016<br />

Lab No<br />

DP1918<br />

Sample<br />

descriptor<br />

Fish brain/ skull<br />

cavity fat<br />

Chemical Result (mg/kg) Ref link<br />

DDE 0.48 #08-185511<br />

DDD 0.08<br />

DDT 0.09<br />

Multi residue<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Limit <strong>of</strong> reporting 0.001 mg/kg <strong>for</strong> cyfluthrin.<br />

Comments: Samples were only analysed <strong>for</strong> cyfluthrin. Lab numbers ending in an ‘R’ are <strong>the</strong><br />

<strong>results</strong> from <strong>the</strong> re-analyses <strong>of</strong> (CRL) 6204. Not re-extracted but reanalysed from previous<br />

extraction. Reported on ‘as received’ basis. It is not stated what containers were used or<br />

how all <strong>the</strong> samples were stored. No in<strong>for</strong>mation included on sampling methodology.<br />

Sampling date is in conflict between report <strong>and</strong> submission <strong>for</strong>m. Greyed out samples not<br />

relevant to sample submission.<br />

February 2009 – Exposure estimate<br />

Fish Health<br />

There do not appear to be <strong>fish</strong> <strong>health</strong> events in this period.<br />

Spray Records<br />

No pesticide applications recorded during this period.<br />

Wea<strong>the</strong>r records<br />

No pesticide applications recorded during this period.<br />

Samples<br />

Sample 1<br />

BSL sample submission <strong>for</strong>m. (Document 204)<br />

Accession No: 09-103703.<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: 11/2/09<br />

Date received: 12/2/09<br />

Animal: mosquito <strong>fish</strong> <strong>and</strong> tadpoles<br />

History <strong>and</strong> clinical signs reported: Investigate pesticide exposure<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1934 Tadpoles (cane toad) unstated OC, OP, SP, o<strong>the</strong>r<br />

Comments:<br />

It is not stated what containers were used or how all <strong>the</strong> samples were stored. No<br />

in<strong>for</strong>mation included on sampling methodology. Some parts <strong>of</strong> <strong>the</strong> <strong>for</strong>m are illegible.<br />

Chemical Residue Laboratory Report 6256 (Document 104)<br />

Date 28 May 2010 Page 65 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Submitter: Roger Chong Date sampled: 11/2/09 Method: AP016<br />

Lab No<br />

DP1934<br />

Sample<br />

descriptor<br />

Tadpoles (cane<br />

toad)<br />

Chemical Result Ref link Submission<br />

(mg/kg)<br />

<strong>for</strong>m<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1933 1 x Feed sample unstated OC, OP, SP, o<strong>the</strong>r<br />

Chemical Residue Laboratory Report 6255 (Document 103)<br />

Submitter: Roger Chong Date sampled: 11/2/09 Method: AP016<br />

Lab No<br />

Sample<br />

Chemical<br />

Result<br />

Ref link<br />

Submission<br />

descriptor<br />

(mg/kg)<br />

<strong>for</strong>m<br />

DP1932<br />

Mosquito <strong>fish</strong><br />

DDE 0.03<br />

#09-103692 204/205<br />

DDT 0.01<br />

Multi residue<br />


SUMMARY OF RESULTS FOR NFHIT<br />

July 2009 – Event 7, Syndrome – De<strong>for</strong>mities.<br />

Fish Health<br />

The following timeline in<strong>for</strong>mation is taken from <strong>the</strong> Noosa Fish Health Investigation Task<br />

Force, 2 nd Veterinary Report, Mullet De<strong>for</strong>mities Syndrome, 29-9-09 (document 601).<br />

Date Reported Event Supporting<br />

evidence<br />

5/7/2009 Spawned 4 female <strong>and</strong> 3 male mullet <strong>fish</strong>es selected from a catch unstated<br />

<strong>of</strong> 16 ton <strong>of</strong> mullet caught by commercial <strong>fish</strong>ers at <strong>the</strong> mouth <strong>of</strong><br />

<strong>the</strong> Noosa River<br />

7/7/09 It was reported that mullet (Mugil cephalus), spawned from Noosa Unstated<br />

River mullet brood<strong>fish</strong> producing a <strong>fish</strong> embryo with ‘3 heads’.<br />

O<strong>the</strong>r embryos had ‘2 heads’ toge<strong>the</strong>r with various ‘cell<br />

abnormalities’.<br />

16/7/09 Fur<strong>the</strong>r mullet were collected from <strong>the</strong> Noosa River. The <strong>fish</strong> were Unstated<br />

divided into a group <strong>of</strong> 2 female travelling mullet © <strong>and</strong> 2<br />

female river mullet (RM) brood females <strong>for</strong> spawning. Four<br />

spawnings were produced, two each from <strong>the</strong> TM <strong>and</strong> RM females.<br />

21/7/2009 The spawnings <strong>of</strong> TM female produced eggs resulted in live <strong>and</strong><br />

viable hatched fry. The spawnings <strong>of</strong> RM female produced eggs<br />

resulted in no live or viable hatched fry.<br />

Unstated<br />

Spray Records<br />

Data taken from Chemical Application Record 2009 Spray Season – Arbuckles Farm<br />

(document 446) <strong>and</strong> Chemical Application Record 2009 (document 447)<br />

Date/time Block/Row Active Equipment Comments<br />

Used<br />

13/7/09 glufosinate Boom<br />

14/7/09 glufosinate Boom<br />

16/7/09 knob, 4<br />

rows, B4<br />

glufosinate Boom wind picked up from <strong>the</strong> west.<br />

Stop spraying at 9:45am, started<br />

at 8:10am<br />

21/7/09 Central & glufosinate Boom Wind E-SE<br />

House<br />

20/7/09 G4, G3 glufosinate Boom Wind – W-SW<br />

Date 28 May 2010 Page 68 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

28/7/09<br />

central, B1,<br />

glufosinate<br />

Boom<br />

start<br />

A1<br />

10:20am<br />

Fin 3:30<br />

31/7/09<br />

B1, A1, B2,<br />

glufosinate<br />

Boom<br />

Start 9:30<br />

B3, A2, A3,<br />

Fin 3:30<br />

GH<br />

Date Block Fertiliser<br />

11/7/09 G5, C1 unstated<br />

Wea<strong>the</strong>r records (Zeisemer)<br />

None recorded<br />

Wea<strong>the</strong>r stations<br />

Tewantin is <strong>the</strong> closest wea<strong>the</strong>r station.<br />

The following is taken from <strong>the</strong> bureau <strong>of</strong> meteorology website <strong>for</strong> July in Tewantin<br />

(http://www.bom.gov.au/climate/dwo/200907/html/IDCJDW4118.200907.shtml)<br />

Day Temp Rain 9am dir 9am 3pm dir 3pm<br />

max ( O C)<br />

speed<br />

speed<br />

(km/h)<br />

(km/h)<br />

1 24.1 0 NNW 9 N 9<br />

2 26.1 0.2 N 6 SSW 6<br />

3 22.1 0 NNW 7 WNW 13<br />

4 21.1 0 WSW 11 WSW 11<br />

5 17.4 0 NNW 9 ESE 4<br />

6 20.2 0 SW 6 SE 7<br />

7 18.1 0 SSW 13 S 19<br />

8 18.8 0 S 26 S 19<br />

9 19.5 0 S 20 S 28<br />

10 0.2 S 28 S 24<br />

11 20.4 SSW 20 SSE 17<br />

12 20.6 0 SW 4 NE 13<br />

13 24 0 NW 11 NW 13<br />

14 21.9 0.6 SW 4 ENE 9<br />

15 20.1 0.2 NW 7 N 11<br />

16 18.4 0 WNW 9 W 13<br />

17 20.1 0 W 7 SSW 11<br />

18 19.4 0 SSW 9 SSE 11<br />

19 22.5 0 NE 4 NE 17<br />

20 22.7 0 SW 7 E 9<br />

Date 28 May 2010 Page 69 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

21 23.2 0 WNW 6 NE 13<br />

22 22.8 0 W 4 N 17<br />

23 24.1 0 NNW 7 NNW 11<br />

24 19.4 0 SSW 13 S 22<br />

25 19 0 S 15 SE 9<br />

26 20.1 0.4 S 9 ENE 7<br />

27 24.5 0.2 NW 13 WNW 9<br />

28 20.6 0 SSW 9 ESE 11<br />

29 21.5 0 S 2 ESE 13<br />

30 20.5 0 Calm E 11<br />

31 21.8 0 WNW 6 NNW 6<br />

Samples<br />

Sample 1<br />

BSL Accession: 09-120470. (Document 215)<br />

Submitter: Mat<strong>the</strong>w L<strong>and</strong>os<br />

Property: Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: 19/7/09-21/7/09<br />

Date received: 22/7/09<br />

Animal: mullet<br />

Follow on from previous mullet egg accession (date 13/7/09). Fur<strong>the</strong>r mullet sourced from<br />

<strong>the</strong> beachfront adjacent <strong>the</strong> mouth <strong>of</strong> <strong>the</strong> Noosa River system. Females induced with LHRHa<br />

50 IU/kg <strong>and</strong> repeat dosed with 25 IU/kg 24 hours later. Sampling <strong>of</strong> eggs <strong>and</strong> embryos to<br />

compare <strong>and</strong> contrast to first mullet spawning <strong>of</strong> <strong>the</strong> season. Reviewing fertisaation rate:<br />

proportion <strong>of</strong> de<strong>for</strong>mities; presence/ absence <strong>of</strong> infectious agents.<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

unstated Various mullet organs Formalin fixed unstated<br />

CRL inter-laboratory sample <strong>for</strong>m (document 215)<br />

Accessions 09-121094 & 09-120470 sent to Simon Christen, QHFSS.<br />

Listed as<br />

Lab Ref Description No Condition/type Analysis Req<br />

09-121094 1 box <strong>fish</strong> samples 10x unstated Residues Noosa Fish<br />

Health Screen<br />

09-120470 1 box <strong>fish</strong> samples 10x unstated Residues Noosa Fish<br />

Health Screen<br />

Comments:<br />

Date 28 May 2010 Page 70 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

The accession 09-121094 sent to QHFSS <strong>for</strong> residue analysis was designated as ‘<strong>for</strong>malin<br />

fixed’. Advice from QHFSS is that <strong>the</strong> samples were not <strong>for</strong>malin fixed.<br />

It is unclear in what condition <strong>the</strong>y were sent or what analysis was requested. Samples were<br />

sent <strong>for</strong> residue analysis.<br />

QHFSS Report 09KE1258-1264, 1328-1330 (document 141)<br />

Submitter: unstated Date sampled: 19-21/07/09<br />

Method: 22276R2, lipid content by solvent extracted gravimetric analysis<br />

QHFSS lab No<br />

10KE1328<br />

10KE1329<br />

10KE1330<br />

10KE1258<br />

10KE1259<br />

10KE1260<br />

10KE1261<br />

10KE1262<br />

10KE1263<br />

10KE1264<br />

Sample<br />

Chemical Result (mg/kg) Ref link<br />

descriptor<br />

B1 NR whole <strong>fish</strong> Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Chlorpyrifos Not tested Fipronil Not tested<br />

DDD pp 0.02 DDE pp 0.02<br />

DDT pp 0.02 Alpha endosulfan 0.02<br />

Beta endosulfan 0.02 Endosulfan sulfate 0.02<br />

Comments: Date received <strong>for</strong> B1-B3 19/2/10 <strong>and</strong> B4-B6 24/11/09. Analysis commenced<br />

19/2/10.<br />

September <strong>and</strong> October 2009 – Background sampling<br />

Protocol: Sampling plan <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation Task<strong>for</strong>ce ver 5 (document<br />

1)<br />

Sample plan 23-9-09 (Document 227)<br />

Sampler: John Nagle<br />

Property: unstated<br />

Date sampled: 23/9/09, 20/10/09<br />

Date: 23/9/09<br />

Source Sample Ref No. Date/Time Sample Type Analysis Reqd<br />

Plankton Pond (A)<br />

JMN230909A01 23.09.09 Water Pesticides<br />

JMN230909A02 23.09.09 Water Pesticides<br />

JMN230909A03 23.09.09 Water Pesticides<br />

JMN230909A04 23.09.09 Filtered Water Metals<br />

JMN230909A05 23.09.09 Sediment Sediments<br />

JMN230909A06 23.09.09 Water Alkalinity<br />

Barra Pond (B) JMN230909B01 23.09.09 Sediment Sediments<br />

Males Pond ©<br />

Hatchery (H)<br />

JMN230909C01 23.09.09 Water Pesticides<br />

JMN230909C02 23.09.09 Water Pesticides<br />

JMN230909C03 23.09.09 Water Pesticides<br />

JMN230909C04 23.09.09 Filtered Water Metals<br />

JMN230909C05 23.09.09 Sediment Sediments<br />

JMN230909C06 23.09.09 Water Alkalinity<br />

JMN230909H01 23.09.09 Water Pesticides<br />

JMN230909H02 23.09.09 Water Pesticides<br />

Source Dam (D) JMN230909D01 23.09.09 Water Pesticides<br />

Date: 20/10/09<br />

Source Sample Ref No. Date/Time Sample Type Analysis Reqd<br />

Plankton Pond (A)<br />

Males Pond ©<br />

JMN201009A07 20.10.09 Water DO<br />

JMN201009A08 20.10.09 Water BOD<br />

JMN201009A09 20.10.09 Fish ???<br />

JMN201009C07 20.10.09 Water DO<br />

JMN201009C08 20.10.09 Water BOD<br />

Date 28 May 2010 Page 72 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

The following documents are also relevant to <strong>the</strong> sample submission to QHFSS but <strong>the</strong>y<br />

have not been summarised as <strong>the</strong>y contain <strong>the</strong> same in<strong>for</strong>mation as above.<br />

1. QHFSS sample submission <strong>for</strong>m <strong>for</strong> JMN090210F01 (document 217)<br />

2. QHFSS sample submission <strong>for</strong>m <strong>for</strong> JMN230909 (document 218)<br />

3. QHFSS sample submission <strong>for</strong>m <strong>for</strong> JMN201009 (document 218)<br />

In-field readings on portable meter (Document 129)<br />

Operative: John Nagle Date sampled: 23/9/09<br />

Pond A (plankton pond)<br />

Location in<br />

West Centre East<br />

Pond<br />

Reading (A or B) A B A B A B<br />

Depth 0.336 0.551 0.365 0.837 0.303 0.715<br />

Temperature 23 23.01 23.18 22.73 23.43 22.7<br />

Dissolved 10.49 10.52 10.64 10.37 10.56 10.02<br />

Oxygen (mg/l)<br />

DO (%) 121.7 122.6 124.5 120.6 123.5 116.2<br />

Turbidity 24.1 27.4 740 846 38.7 43.1<br />

Conductivity 102 102 102 102 102 102<br />

pH 8.1 7.87 8.23 7.74 8.21 7.37<br />

Pond C (males pond)<br />

Location in Pond Peg 1 Peg 2 Peg 3 Peg 4<br />

Reading (A or B) A B A B A B A B<br />

Depth 0.24 0.428 0.19 0.502 0.328 0.605 0.281 0.467<br />

Temperature 23.17 22.67 23.13 22.3 23.06 22.04 23.64 22.39<br />

Dissolved Oxygen<br />

mg/L 8.49 7.32 8.23 6.05 6.89 3.79 6.82 4.41<br />

DO (%) 98.9 85.4 97.5 71.4 82.3 45.1 81.5 53<br />

Turbidity 20.2 95.6 12.2 31.7 30.5 26.4 20 35.2<br />

Conductivity 351 350 343 344 344 345 343 343<br />

pH 5.91 5.76 5.75 5.71 5.96 5.71 6.01 5.9<br />

QHFSS Report 09NA-12984-09NA12985:TKP2 (Document 128)<br />

Submitter: John Nagle Date sampled: 23/9/09 Method: 18208<br />

Lab No Sample Analyte Result Ref link<br />

descriptor<br />

09NA12984 pond water Conductivity (uS/cm 100 JMN230909A01_6<br />

Date 28 May 2010 Page 73 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

@25 o c)<br />

pH 6.97<br />

Alkalinity (mg/L<br />

19<br />

CaCO 3)<br />

09NA12985<br />

pond water<br />

Conductivity (uS/cm<br />

350<br />

JMN230909C01_6<br />

@25 o c)<br />

pH 6.96<br />

Alkalinity (mg/L<br />

40<br />

CaCO 3)<br />

Method: 18196<br />

Analyte<br />

Results (mg/L)<br />

Water pond Water pond<br />

JMN230909A01_6 JMN230909C01_6<br />

Aluminium


SUMMARY OF RESULTS FOR NFHIT<br />

QHFSS Report 09KE6901-09KE6908: pw1 (Document 150)<br />

Submitter: John Nagle Date sampled: 23/9/09 method:QIS16315<br />

Analysis: pesticide residues<br />

Lab No Descriptor Chemical Result (ug/L) Ref link<br />

09KE6901 Pond water Nonylphenol 0.039 JMN230909A01_6<br />

Multi residue<br />

(o<strong>the</strong>r)<br />


SUMMARY OF RESULTS FOR NFHIT<br />

QHFSS Report 09KE6901-6908 (Document 148) <strong>and</strong> amended QHFSS Report 09KE6901-<br />

6908 (document 149)<br />

Submitter: John Nagle Date sampled: 23/09/09 Method: 1) analysis <strong>of</strong><br />

soils <strong>and</strong> sediments <strong>for</strong> herbicides by LCMS 2) Determination <strong>of</strong> Organochlorines,<br />

Organophosphorus, Syn<strong>the</strong>tic Pyrethroids <strong>and</strong> Polychlorinated Biphenyls in soil <strong>and</strong><br />

sediments.<br />

QHFSS lab No<br />

09KE6902<br />

09KE6903<br />

09KE6905<br />

09KE6906<br />

Sample<br />

descriptor<br />

Pond sediment<br />

Pond sediment<br />

Pond sediment<br />

Pond sediment<br />

Chemical Result (mg/kg) Ref link<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Dissolved<br />

7.7<br />

oxygen**<br />

EN3983<br />

unstated<br />

BOD 5 days 20 o C


SUMMARY OF RESULTS FOR NFHIT<br />

QHFSS Report 10KE1061 (Document 146)<br />

Submitter: John Nagle Date sampled: 9/2/10<br />

Method: sample extraction with dichloromethane using ultrasonic bath, followed by<br />

evaporation under vacuum <strong>and</strong> analysis by GCMS.<br />

Analysis: unstated<br />

Sample: Filter paper (23.5 cm diameter) reported to be 0.043m 2 by QHFSS.<br />

Lab No Descriptor Chemical Result<br />

(ug/filter)<br />

09KE8072 Filter paper nonylphenol 0.630<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

– 13:25<br />

Reported Event<br />

4/9/09 Phone message notification provided. Flag signal hoisted to indicate possible<br />

spraying in 2 hours.<br />

5/9/09 Most <strong>of</strong> day observed to be spraying. Rainfall followed that night. Wind<br />

conditions at risk <strong>of</strong> spray drift observed about 90% <strong>of</strong> <strong>the</strong> time – North (N) to<br />

south (S) direction towards hatchery. Spraying along North boundary <strong>of</strong> hatchery<br />

greater than 4 rows <strong>of</strong> trees buffered.<br />

6/9/09 14:49- Spraying observed in <strong>the</strong> afternoon. Wind conditions at risk <strong>of</strong> spray drift<br />

19:56 observed about 90% <strong>of</strong> <strong>the</strong> time – N to S direction towards hatchery. Spraying<br />

along North boundary <strong>of</strong> hatchery, greater than 4 rows <strong>of</strong> trees buffered. Night<br />

time wind W to E direction, spraying along west boundary <strong>of</strong> hatchery, greater<br />

than 4 rows <strong>of</strong> trees buffered.<br />

10/9/09 No notification <strong>of</strong> spraying provided. Spraying along North boundary <strong>of</strong> hatchery<br />

at 16:19, close to 3 or 4 rows buffered. Wind N to S direction towards hatchery.<br />

After dark, collected algae <strong>and</strong> water from <strong>the</strong> algal tank to feed remaining bass<br />

fry in <strong>the</strong> hatchery. The algal tank is situated outside <strong>the</strong> hatchery on <strong>the</strong><br />

Nor<strong>the</strong>rn aspect <strong>of</strong> <strong>the</strong> hatchery shed <strong>and</strong> is uncovered. Tank 1 <strong>of</strong> bass fry with<br />

about 500 fry received 10L <strong>of</strong> algae with water from <strong>the</strong> algal tank. Tank 2 <strong>of</strong><br />

bass fry with about 3,000 – 4,000 fry received 20L <strong>of</strong> algae with water. No<br />

molasses or urea or fertiliser were added in <strong>the</strong> operation <strong>of</strong> <strong>the</strong> algal culture in<br />

<strong>the</strong> outside tank.<br />

11/9/09 08:00 Tank 1 <strong>of</strong> bass fry with about 500 fry received 10L <strong>of</strong> algae with water<br />

from <strong>the</strong> algal tank. Tank 2 <strong>of</strong> bass fry with about 3,000 – 4,000 fry received 20L<br />

<strong>of</strong> algae with water. Both tanks had 4000L <strong>of</strong> water.<br />

12/9/09 No spraying. Tank 1 <strong>of</strong> bass fry with about 500 fry received 10L <strong>of</strong> algae with<br />

water from <strong>the</strong> algal tank. Tank 2 <strong>of</strong> bass fry with about 3,000 – 4,000 fry<br />

received 20L <strong>of</strong> algae with water.<br />

13/9/09 09:00 75% mortalities <strong>of</strong> bass fry in tank 1, 100% mortalities <strong>of</strong> bass fry in tank 2.<br />

No mortalities observed in bass fry held in <strong>the</strong> <strong>the</strong> outside tanks. These tanks<br />

are situated on <strong>the</strong> south aspect <strong>of</strong> <strong>the</strong> hatchery shed which have a cover over<br />

75% <strong>of</strong> <strong>the</strong> tanks’ opened surface. The bass were not fed with algae but with<br />

yeast. All o<strong>the</strong>r bass fry which were relocated to <strong>the</strong> Ringtail ponds prior<br />

to spray activity do not show any abnormalities. Algae used were cultured from<br />

strains <strong>of</strong> algal provided by <strong>the</strong> Queensl<strong>and</strong> Primary Industries <strong>and</strong><br />

Fisheries, Bribie Isl<strong>and</strong> Aquaculture Research Centre. These algae had been<br />

used to grow <strong>the</strong> bulk <strong>of</strong> <strong>the</strong> normal bass fry which were transported to <strong>the</strong><br />

Ringtail ponds.<br />

Spray records<br />

Data taken from Chemical Application Record 2009 Spray Season – Arbuckles Farm<br />

(document 446) <strong>and</strong> chemical application record 2009 (document 447)<br />

Date 28 May 2010 Page 79 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date/time Block* Active Equipment used<br />

6/09/09<br />

S – 9.45am<br />

F-11pm<br />

B2, B3, G1,<br />

G5,G7, C1, C2,<br />

C3, Knob, GH,<br />

B4<br />

methoxyfenozide<br />

nonylphenol<br />

ethoxylate<br />

condensate<br />

Airblast<br />

*The blocks numbers are difficult to read in <strong>the</strong> original document. The blocks written above are Ian<br />

Walker’s interpretation <strong>of</strong> <strong>the</strong> h<strong>and</strong> writing.<br />

Date Block Fertiliser<br />

8/9/09 The lot Easy N<br />

Wea<strong>the</strong>r records (Zeisemer)<br />

Date Time Hum Temp Wind Wind Block Comments<br />

(%) ( O C) Speed<br />

(km/h)<br />

Direction<br />

6/9/09 9:15am 73.3 21 0-3 E G8 Flag up 7:10am – all good<br />

12:00pm 51.3 26.6 0-5 ENE var G6<br />

2:00pm 51.9 28 0-5 ENE var G5<br />

4:30pm 71.2 22.5 0-3 ENE var B4<br />

5:55pm 66.7 22.9 0.1 N/NW Knob<br />

7:10pm 69.8 20.5 N/NW B2, B3<br />

10:05pm 69.4 21.7 GH<br />

10:55pm<br />

Finish flag down 11:00pm<br />

Samples<br />

BSL sample submission <strong>for</strong>m. (Document 228) <strong>and</strong> attachment (document 232)<br />

Sample No: 09-126853<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Hatchery<br />

Date sampled: unstated<br />

Date received: 18/9/09<br />

Sample: Fish <strong>and</strong> poultry<br />

History <strong>and</strong> clinical signs reported: see attached history sheet.<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

Unstated F1 7/9/09 large block tank<br />

tag 000100<br />

Unstated Pesticides<br />

Unstated<br />

F2 7/9/09 Plankton pond tag<br />

000039<br />

Unstated/<br />

cracked not<br />

Pesticides<br />

Date 28 May 2010 Page 80 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Lab Ref Description Condition/type Analysis Req<br />

leaking<br />

Unstated F3 ??? tank 2 tag 00089 Unstated Pesticides<br />

QHFSS Laboratory Report 09ke6807-09ke6809: pw1 (Document 127)<br />

Submitter: unstated Date sampled: 18/09/09<br />

Method: methoxyfenozide (LCMS), urea ENM-009<br />

lab No<br />

Sample<br />

descriptor<br />

09ke6807 F1, tag 00100<br />

09ke6808 F1, tag 0099<br />

09ke6809 F1, tag 0089<br />

Chemical Result<br />

(ug/L)<br />

methoxyfenozide 0.001<br />

Urea as N unstated<br />

methoxyfenozide


SUMMARY OF RESULTS FOR NFHIT<br />

Date Reported Event<br />

unstated The Barra pond (Figure 1), had been purposefully dredged <strong>and</strong> refilled with new<br />

water prior to stocking <strong>and</strong> monitoring. A plankton bloom in <strong>the</strong> pond was<br />

established prior to <strong>fish</strong> introduction.<br />

20/10/09 Golden perch (Macquaria ambigua) spawned<br />

he neighbouring macadamia farm was reported by <strong>the</strong> hatchery. Spraying<br />

activity was reported to have occurred during variable wind conditions.<br />

11/11/09 Of <strong>the</strong> 10,000 golden perch fry stocked in <strong>the</strong> Barra pond, no dead <strong>fish</strong> were<br />

observed but only 2 live <strong>fish</strong> were visualised remaining in <strong>the</strong> pond. These two<br />

<strong>fish</strong> were sampled <strong>for</strong> laboratory examination. That is, <strong>the</strong>re was an observation<br />

<strong>of</strong> greater than 99% loss <strong>of</strong> <strong>the</strong> stocked population <strong>of</strong> golden perch fry.<br />

Observation on <strong>the</strong> water in <strong>the</strong> Barra pond did not indicate <strong>the</strong> presence <strong>of</strong><br />

predators such as rodents, large <strong>fish</strong> or ulphaten (redclaw) which could have<br />

simply explained <strong>the</strong> disappearance <strong>of</strong> <strong>fish</strong> over a period <strong>of</strong> 17 days.<br />

13/11/09 Of <strong>the</strong> cohorts stocked at Ringtail pond red 7, a survey sampling produced 300<br />

live golden perch <strong>fish</strong>.<br />

4/2/2010 Fur<strong>the</strong>r sampling at Ringtail produced 107 <strong>fish</strong> from a r<strong>and</strong>om netting.<br />

Comments: It is unstated how <strong>the</strong> surveys were conducted. It is unclear whose observation<br />

was recoded <strong>for</strong> <strong>the</strong> 11/11/09.<br />

Spray records<br />

Data taken from Chemical Application Record 2009 Spray Season – Arbuckles Farm<br />

(document 446) <strong>and</strong> chemical application record 2009 (document 447)<br />

Date/time Block Active Equipment used<br />

2/11/09<br />

Central<br />

Trichlorfon<br />

Airblast<br />

S-4.10pm<br />

A1,B1,C3<br />

soyal<br />

F-6:30pm<br />

phospholipids +<br />

propionic acid<br />

3/11/09<br />

A1,B1,C1,C2,C3,Knob,B4,A2,A3,B2,B3,<br />

Trichlorfon<br />

Airblast<br />

S -9.40am<br />

G6,G2<br />

soyal<br />

F-8.40pm<br />

phospholipids +<br />

propionic acid<br />

4/11/09<br />

G5,G2, Central, GH<br />

Trichlorfon<br />

Airblast<br />

S-9.30am<br />

soyal<br />

F-3.40pm<br />

phospholipids +<br />

propionic acid<br />

5/11/09<br />

G1,GH,G3,G4<br />

Trichlorfon<br />

Airblast<br />

S-7.45am<br />

soyal<br />

F-1.30pm<br />

phospholipids +<br />

propionic acid<br />

Date 28 May 2010 Page 82 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

11/11/09<br />

G1,G3,G4<br />

Trichlorfon<br />

Airblast<br />

S-8.20am<br />

soyal<br />

F-12.30pm<br />

phospholipids +<br />

propionic acid<br />

25/11/09<br />

G1,G3,G4<br />

Beta-cyfluthrin<br />

Airblast<br />

S-8.15am<br />

nonylphenol<br />

F-4.00pm<br />

ethoxylate<br />

condensdate<br />

26/11/09<br />

G4,G5<br />

Beta-cyfluthrin<br />

Airblast<br />

S-7.00am<br />

nonylphenol<br />

F-4.00pm<br />

ethoxylate<br />

condensdate<br />

27/11/09<br />

Central, C3, B2<br />

Beta-cyfluthrin<br />

Airblast<br />

S-11.40am<br />

nonylphenol<br />

F-1.00am -<br />

ethoxylate<br />

28/11/09<br />

condensdate<br />

Date/time Block Active Equipment used<br />

18/11/09<br />

S-11:30am<br />

F- 3:30pm<br />

19/11/09<br />

S-7:00am<br />

F-3:30pm<br />

20/11/09<br />

S-7:00am<br />

30/11/09<br />

S-2:30pm<br />

F-3:30pm<br />

G5 Basta h<strong>and</strong> gun<br />

G5 Basta Boom<br />

G7 part <strong>of</strong> Basta boom<br />

part G7 Basta h<strong>and</strong>gun<br />

Date Block Fertiliser<br />

5/11/09 The lot Black urea<br />

Wea<strong>the</strong>r records (Zeisemer)<br />

Date Time Hum Temp Wind Wind Block Comments<br />

(%) ( O C) Speed<br />

(km/h)<br />

Direction<br />

2/11/09 4:10pm 61.9 27 3-10 NE Central Start flg up 11:50am<br />

4:20pm<br />

Stop central<br />

6:10pm 57.4 21.7 3.2 NE A1, B1 Start<br />

6.35pm 65.3 20.9 0.3 NE A1,B1, C3 Stop<br />

Date 28 May 2010 Page 83 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Time Hum Temp Wind Wind Block Comments<br />

(%) ( O C) Speed<br />

(km/h)<br />

Direction<br />

3/11/09<br />

9:40am 51.3 26.7 19 NE<br />

B1, A1,<br />

C1, Knob<br />

10:40am 48.6 27.6 2.8-3.9 NE Knob<br />

11:00am 43.2 27.5 0 – 7 NNE A2<br />

12:40pm 43.7 28.1 0-6.4 NE-SE B2, B3<br />

1:00pm 47.1 30 1.9-9.8 E-SE B1,A1<br />

4:30pm 52.2 27.8 5-10 E G6<br />

5:40pm 0 Finish flag down 5:50<br />

4/11/09<br />

9:30am 44.8 26.8 0 – 8<br />

N-<br />

oscilating G2,G5 Start Flag up 7:15<br />

10:40pm 0.1<br />

N (NNE-<br />

NW)<br />

Stopped spraying<br />

12:35pm 44.7 33.9 0 – 5 E<br />

Central,<br />

G5 Started<br />

1:09pm 47.6 30 4 – 11 E<br />

Central,<br />

G5<br />

1:35pm 51.8 29.5 3 – 9 E/NE<br />

Central,<br />

G5<br />

2:07pm 59.2 30.2 3.6 – 8 ESE<br />

Central,<br />

G5<br />

2:55pm 55.6 30 3 – 8 E<br />

Central ,<br />

GH<br />

3:20pm GH Finished central<br />

3:40pm NW GH<br />

Stopped spraying, flag<br />

down 4:15<br />

5/11/09<br />

7:45am 58.5 25.5 0-5 NW GH,G3,G4<br />

Starting Flag up<br />

4:50am<br />

8:00am<br />

up to<br />

23 N stopped<br />

1:00am 46 33.7<br />

5.3<br />

max E G1 Starting<br />

1:30am<br />

Stopped too gusty<br />

6/11/09 9:20am 50 27.1 0-6 E G1 Starting flag up 4:40<br />

9:35am 9 N Gusts Stopped wind changed<br />

11/11/09 8:20am 53.6 24.4 0-5 SE G1 Starting flag up 6:20<br />

10:20am 39.4 26 0-8 ESE<br />

12:30am 35.4 27.3 0-10 ESE G1<br />

Finished 1:30pm flag<br />

down 2:20pm<br />

25/11/09<br />

8:15am 58.9 24.2<br />

up to<br />

10.4 E-SE<br />

G1, G3,<br />

G7 Starting flag up 4:50am<br />

9:30am 60.3 25.5<br />

7.1<br />

max ESE<br />

G1, G3,<br />

G7<br />

Date 28 May 2010 Page 84 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date Time Hum Temp Wind Wind Block Comments<br />

(%) ( O C) Speed<br />

(km/h)<br />

Direction<br />

10:25am 56 27.4 0-5.7 ESE<br />

G1, G3,<br />

G4<br />

11:30am 51 29.3 1.7-3.8 ESE<br />

G1, G3,<br />

G4<br />

12:30pm 49 30.2 0-6 ESE<br />

G1, G3,<br />

G4<br />

1:35pm 49.5 30.1 0-8 ESE G1<br />

2:35pm 54.9 29.2 0-10 ESE G1<br />

3:50pm 50.5 28.3 0-7 ESE G4<br />

26/11/09 7:20am 71.1 25.2 0-5 ESE var G4 Flag up 5:00am<br />

8:40am 69 25.8 0-5 E G5<br />

12:00pm 43.6 30 3-10 ENE var<br />

2:30pm 46.5 28 3-8 ENE var<br />

27/11/09 11:40am 43.8 30.4 2.4-10 NE Central<br />

12:20pm 48.2 31.3<br />

3.9-<br />

10.9 NE St<strong>and</strong> pipe<br />

1:30pm 57.2 29.5 0.4-11 NE “ “<br />

2:30pm 58 29.8<br />

1.7-<br />

12.2 ENE Top ridge<br />

3:40pm 55.6 29 4-13 NE “ “<br />

5:00pm 54 28.5 2-11 NE<br />

6:00pm 58.5 26.8 .7-8 NE<br />

7:45pm 62 25.7 0-1 NE C3<br />

10:10pm 57 24.1 0-1 NE B2, B3<br />

11:20pm 53.8 23.2 0-2.1 NNE Knob<br />

1:00am<br />

Finished<br />

DERM wea<strong>the</strong>r station data<br />

Technicians from <strong>the</strong> Department <strong>of</strong> Environment <strong>and</strong> Resource Management (DERM<br />

installed a pole mounted mobile wea<strong>the</strong>r station on <strong>the</strong> property <strong>of</strong> Sunl<strong>and</strong> Fish Hatchery.<br />

First data recording was on <strong>the</strong> 12 October 2009.<br />

Spray applications have been recorded <strong>for</strong> approximately <strong>the</strong> following times. Data is taken<br />

from ‘Spray Times 31-8-09 – 28-11-09’ (Document 437):<br />

Date Activity Time from Chemical<br />

Source<br />

Application Record<br />

2/11/2009 Ag chemical 4:10 pm – 6:35 pm Spray Records<br />

3/11/2009 Ag chemical 9:40 am – 5:40 pm Spray records<br />

4/11/2009 Ag chemical 9:30 am – 3:40 pm Spray records<br />

5/11/2009 Ag chemical 7:45 am – 1:30 pm Spray records<br />

5/11/2009 Fertilizer Ian Walker email (9/12/2009)<br />

Date 28 May 2010 Page 85 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

6/11/2009 Ag chemical 9:20 – 9:35 Spray records<br />

11/11/2009 Ag chemical 8:20 am – 1:30 pm Spray records<br />

25/11/2009 Ag chemical 8:15 am – 4:00 pm Spray records<br />

26/11/2009 Ag chemical 7:00 am – 4:00 pm Spray records<br />

27/11/2009 Ag chemical 11:40 am – 1:00 am (28/11/2009) Spray records<br />

The relative frequency <strong>of</strong> wind speed <strong>and</strong> direction (5-minute averages) have been prepared<br />

by DERM into <strong>the</strong> following summaries (summaries <strong>and</strong> windroses – document 445). The<br />

summaries <strong>and</strong> windroses were prepared based on <strong>the</strong> Pomona Meteorological Data (5-<br />

minute averages) – 12 October to 4 February 2010 (Document 451). The exact location <strong>of</strong><br />

<strong>the</strong> wea<strong>the</strong>r station on <strong>the</strong> property is Latitude: -26.3126 (26 o 18’ 45” S), Longitude:<br />

152.9534 (152 o 57’ 12” E).<br />

2 November 2009 – 4:10pm to 6:35pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

3.4 10.3 13.8 48.3 20.7 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 96.6<br />

0.0 0.0 0.0 0.0 0.0 3.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 3.4<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 3.4 10.3 13.8 48.3 20.7 3.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

3 November 2009 – 9:40am to 5:40pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

5.2 4.2 5.2 22.9 26.0 3.1 3.1 0.0 0.0 0.0 0.0 0.0 1.0 0.0 4.2 1.0 76.0<br />

0.0 0.0 1.0 8.3 11.5 3.1 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 24.0<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 5.2 4.2 6.3 31.3 37.5 6.3 3.1 0.0 0.0 0.0 0.0 0.0 1.0 0.0 4.2 1.0 100.<br />

Date 28 May 2010 Page 86 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Calm periods: 0.0<br />

0<br />

4 November 2009 – 9:30am to 6:30pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

7.4 24.1 10.2 6.5 8.3 0.9 0.0 0.0 0.0 0.0 0.9 0.0 0.9 1.9 4.6 7.4 73.1<br />

0.0 0.0 0.9 2.8 12.0 8.3 0.0 0.0 0.0 0.0 0.0 0.0 1.9 0.9 0.0 0.0 26.9<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 7.4 24.1 11.1 9.3 20.4 9.3 0.0 0.0 0.0 0.0 0.9 0.0 2.8 2.8 4.6 7.4 100.<br />

Calm periods: 0.0<br />

0<br />

5 November 2009 – 7:45am to 1:30pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

14.5 15.9 1.4 7.2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 5.8 7.2 52.2<br />

5.8 1.4 0.0 5.8 30.4 1.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 44.9<br />

0.0 0.0 0.0 0.0 0.0 2.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.9<br />

TOTAL 20.3 17.4 1.4 13.0 30.4 4.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 5.8 7.2 100.<br />

Calm periods: 0.0<br />

0<br />

6 November 2009 – 9:20am to 9:35am<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 66.7 33.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

0<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

Date 28 May 2010 Page 87 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

TOTAL 0.0 0.0 66.7 33.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

7 November 2009 – 7:25am to 3:509m<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 2.0 10.9 23.8 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 37.6<br />

0.0 0.0 1.0 5.0 29.7 26.7 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 62.4<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 3.0 15.8 53.5 27.7 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

Date 28 May 2010 Page 88 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

11 November 2009 – 8:20am to 1:30pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 1.6 3.2 1.6 0.0 0.0 1.6 0.0 0.0 0.0 0.0 0.0 0.0 0.0 8.1<br />

0.0 0.0 0.0 0.0 1.6 64.5 24.2 1.6 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 91.9<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 0.0 1.6 4.8 66.1 24.2 1.6 1.6 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

25 November 2009 – 8:00am to 5:00pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 0.0 4.6 1.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 6.5<br />

0.0 0.0 0.0 2.8 25.9 52.8 8.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 89.8<br />

0.0 0.0 0.0 0.0 0.0 2.8 0.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 3.7<br />

TOTAL 0.0 0.0 0.0 2.8 30.6 57.4 9.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

26 November 2009 – 7:00am to 4:00pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

3.7 3.7 1.9 12.0 7.4 0.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.9 30.6<br />

0.0 0.0 4.6 49.1 15.7 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 69.4<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 3.7 3.7 6.5 61.1 23.1 0.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.9 100.<br />

Calm periods: 0.0<br />

0<br />

Date 28 May 2010 Page 89 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

27 November 2009 – 8:30am to 1:30am (28 November)<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

12.2 14.7 3.2 9.6 6.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.3 1.9 8.3 5.1 62.8<br />

0.0 0.0 0.0 10.9 21.2 2.6 0.0 0.0 0.0 0.0 0.0 0.6 0.0 1.3 0.0 0.0 36.5<br />

0.0 0.0 0.0 0.0 0.0 0.6 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.6<br />

TOTAL 12.2 14.7 3.2 20.5 27.6 3.2 0.0 0.0 0.0 0.0 0.0 0.6 1.3 3.2 8.3 5.1 100.<br />

Calm periods: 0.0<br />

0<br />

1 December 2009 – 7:30am to 11:00am<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

0.0 0.0 0.0 0.0 0.0 11.9 50.0 26.2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 88.1<br />

0.0 0.0 0.0 0.0 0.0 11.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 11.9<br />

TOTAL 0.0 0.0 0.0 0.0 0.0 23.8 50.0 26.2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

9 December 2009 – 8:30am to 4:00pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

8.9 6.7 4.4 7.8 18.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.2 14.4 8.9 72.2<br />

4.4 1.1 0.0 0.0 4.4 4.4 0.0 0.0 0.0 0.0 0.0 0.0 1.1 5.6 5.6 1.1 27.8<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 13.3 7.8 4.4 7.8 23.3 4.4 0.0 0.0 0.0 0.0 0.0 0.0 1.1 7.8 20.0 10.0 100.<br />

Calm periods: 0.0<br />

0<br />

Date 28 May 2010 Page 90 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

11 December 2009 – 7:30am to 12:00pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

5.6 5.6 7.4 0.0 0.0 0.0 0.0 0.0 1.9 0.0 0.0 0.0 1.9 13.0 18.5 29.6 83.3<br />

0.0 0.0 0.0 0.0 1.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 7.4 7.4 0.0 16.7<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 5.6 5.6 7.4 0.0 1.9 0.0 0.0 0.0 1.9 0.0 0.0 0.0 1.9 20.4 25.9 29.6 100.<br />

Calm periods: 0.0<br />

0<br />

12 January 2010 – 7:00am to 3:30pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 1.0 10.8 24.5 14.7 6.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 57.8<br />

0.0 0.0 0.0 1.0 3.9 20.6 14.7 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 42.2<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 0.0 2.0 14.7 45.1 29.4 8.8 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

13 January 2010 – 7:00am to 3:30pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

4.9 8.8 17.6 27.5 17.6 3.9 1.0 2.9 3.9 1.0 4.9 1.0 0.0 0.0 0.0 0.0 95.1<br />

0.0 0.0 0.0 2.9 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 4.9<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 4.9 8.8 17.6 30.4 17.6 5.9 1.0 2.9 3.9 1.0 4.9 1.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

Date 28 May 2010 Page 91 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

14 January 2010 – 7:00am to 3:30pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 2.0 4.9 9.8 14.7 10.8 8.8 8.8 2.0 4.9 0.0 0.0 0.0 1.0 0.0 67.6<br />

0.0 0.0 0.0 2.9 11.8 12.7 2.9 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 32.4<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 2.0 7.8 21.6 27.5 13.7 10.8 8.8 2.0 4.9 0.0 0.0 0.0 1.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

15 January 2010 – 7:00am to 3:30pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 0.0 1.0 14.7 5.9 1.0 3.9 1.0 1.0 2.9 1.0 0.0 0.0 0.0 32.4<br />

0.0 0.0 0.0 0.0 5.9 35.3 21.6 4.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 67.6<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 0.0 0.0 6.9 50.0 27.5 5.9 3.9 1.0 1.0 2.9 1.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

14 February 2010 – 6:00am to 5:00pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

15.2 13.6 6.1 18.9 23.5 5.3 1.5 0.8 0.8 0.0 0.0 0.0 0.8 2.3 1.5 4.5 94.7<br />

0.0 0.0 0.0 0.8 4.5 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 5.3<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 15.2 13.6 6.1 19.7 28.0 5.3 1.5 0.8 0.8 0.0 0.0 0.0 0.8 2.3 1.5 4.5 100.<br />

Calm periods: 0.0<br />

0<br />

Date 28 May 2010 Page 92 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

15 February 2010 – 7:00am to 9:30am<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

20.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 36.7 26.7 13.3 96.7<br />

3.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 3.3<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 23.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 36.7 26.7 13.3 100.<br />

Calm periods: 0.0<br />

0<br />

20 February 2010 – 7:00am to 9:50am<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 0.0 0.0 0.0 5.9 41.2 17.6 8.8 2.9 0.0 0.0 0.0 0.0 0.0 76.5<br />

0.0 0.0 0.0 0.0 0.0 2.9 14.7 5.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 23.5<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 0.0 0.0 0.0 2.9 20.6 47.1 17.6 8.8 2.9 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

21 February 2010 – 6:00am to 9:00am<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 0.0 0.0 0.0 11.1 5.6 30.6 2.8 16.7 22.2 11.1 0.0 0.0 0.0 100.<br />

0<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 0.0 0.0 0.0 0.0 11.1 5.6 30.6 2.8 16.7 22.2 11.1 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

Date 28 May 2010 Page 93 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

22 February 2010 – 9:30am to 3:00pm<br />

Wind<br />

N NNE NE ENE E ESE SE SSE S SS<br />

SW WS<br />

W<br />

WN<br />

NW NN<br />

All<br />

speed<br />

W<br />

W<br />

W<br />

W<br />

>0 to 2<br />

m/s<br />

>2 to 4<br />

m/s<br />

>4 to 6<br />

m/s<br />

0.0 0.0 0.0 0.0 0.0 0.0 3.0 34.8 40.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 78.8<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 18.2 3.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 21.2<br />

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

TOTAL 0.0 0.0 0.0 0.0 0.0 0.0 3.0 53.0 43.9 0.0 0.0 0.0 0.0 0.0 0.0 0.0 100.<br />

Calm periods: 0.0<br />

0<br />

The Windroses on <strong>the</strong> following pages have been prepared by DERM <strong>for</strong> <strong>the</strong> times above.<br />

Date 28 May 2010 Page 94 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

2 November 2009<br />

4:10pm to 6:35pm<br />

3 November 2009<br />

9:40am to 6:35pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

4 November 2009<br />

9:30am to 6:30pm<br />

5 November 2009<br />

7:45am to 1:30pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

6 November 2009<br />

9:20am to 9:35am<br />

7 November 2009<br />

7:25am to 3:50pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

N<br />

W + E<br />

S<br />

0 2 4 6 m/s<br />

10<br />

Date 28 May 2010 Page 95 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

11 November 2009<br />

8:20am to 1:30pm<br />

25 November 2009<br />

8:00am to 5:00pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

26 November 2009<br />

7:00am to 4:00pm<br />

27 November 2009<br />

8:30am to 1:30am (28 Nov)<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

1 December 2009<br />

7:30am to 11:00am<br />

20%<br />

15%<br />

10%<br />

5%<br />

9 December 2009<br />

8:30am to 4:00pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

N<br />

W + E<br />

S<br />

0 2 4 6 m/s<br />

10<br />

Date 28 May 2010 Page 96 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

11 December 2009<br />

7:30am to 12:00pm<br />

12 January 2009<br />

7:00am to 3:30pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

13 January 2010<br />

7:00am to 3:30pm<br />

14 January 2010<br />

7:00am to 3:30pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

15 January 2010<br />

7:00am to 3:30pm<br />

14 February 2010<br />

6:00am to 5:00pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

N<br />

W + E<br />

S<br />

0 2 4 6 m/s<br />

10<br />

Date 28 May 2010 Page 97 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

15 February 2010<br />

7:00am to 9:30am<br />

20 February 2010<br />

7:00am to 9:50am<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

21 February 2010<br />

6:00am to 9:00am<br />

22 February 2010<br />

9:30am to 3:00pm<br />

20%<br />

15%<br />

10%<br />

5%<br />

20%<br />

15%<br />

10%<br />

5%<br />

N<br />

W + E<br />

S<br />

0 2 4 6 m/s<br />

10<br />

Samples<br />

Advice from Roger Chong is <strong>the</strong> submitter field on <strong>the</strong> BSL sample submission <strong>for</strong>ms does<br />

not indicate who did <strong>the</strong> sampling. Gwen Gilson conducted <strong>the</strong> sampling <strong>for</strong> <strong>the</strong> samples<br />

collected in November 2009.<br />

BSL sample submission <strong>for</strong>m. (Document 220)<br />

Date 28 May 2010 Page 98 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Sample No: 09-132761<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Hatchery<br />

Date sampled: 3/11/09 12:30 am<br />

Date received: 11/11/09<br />

sample: water <strong>and</strong> yellow belly <strong>fish</strong><br />

History <strong>and</strong> clinical signs reported: spraying on <strong>the</strong> day <strong>of</strong> collection. Variable wind<br />

conditions<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

unstated Blocked tank tq92 unstated pesticides<br />

unstated Blocked tank tq91 Unstated/ cracked<br />

unstated<br />

Unstated<br />

Yellow belly <strong>fish</strong> 28/10/09 barra<br />

pond<br />

Yellow belly <strong>fish</strong> 25/10/09 barra<br />

pond<br />

not leaking<br />

unstated<br />

unstated<br />

pesticides<br />

Histology?<br />

Histology?<br />

Comments: It is not stated what containers were used or how all <strong>the</strong> samples were stored.<br />

No in<strong>for</strong>mation included on sampling methodology.<br />

QHFSS Report 09KE8064-8071:SBC (Document 130) <strong>and</strong> QHFSS Report 09KE8064-<br />

8071:SBC amended (Document 152)<br />

Submitter: Roger Chong<br />

Date sampled: unstated<br />

Method: 25391 EMN-009 (advice from Mary Hodge, QHFSS was that <strong>the</strong> method is<br />

segmented flow analysis with diacetyl monoxime (colourimetric determination).<br />

Analysis: endocrine disrupting chemicals<br />

lab No<br />

09KE8604<br />

09KE8605<br />

Sample<br />

descriptor<br />

Block tank<br />

Block tank<br />

Chemical Result (ug/L) Ref link<br />

4-t-octylphenol 0.007 Seal 000091/92<br />

Nonylphenol (tech) 0.510 09-132761<br />

Bisphenol A 0.087<br />

Cholesterol (non<br />

EDC)<br />

0.120<br />

EDC (o<strong>the</strong>rs)<br />


SUMMARY OF RESULTS FOR NFHIT<br />

LOR<br />

Samples were analysed <strong>for</strong> a number <strong>of</strong> Endocrine Disrupting Chemicals with LORs 1- 10<br />

ug/L except nonylphenol with an LOR <strong>of</strong> 50 ug/L because <strong>of</strong> a matrix interference.<br />

Comment: it appears <strong>the</strong> nonylphenol has been analysed against a st<strong>and</strong>ard <strong>of</strong> technical<br />

nonylphenol.<br />

Analysis: urea<br />

Lab No Descriptor Urea (ug/L as N) Ref link<br />

09KE8064 Block tank 11 09-132761 (1&2) (seal 000091/92)<br />

09KE8065 Block tank 30 09-132776 (1&2) (seal 000093/4)<br />

09KE8066 Barra Pond 7 09-132784 (seal 000095)<br />

09KE8067 Block tank 68 09-132784 (seal 000096)<br />

09KE8068 Barra Pond 11 09-132792 (seal 000161)<br />

09KE8069 Block tank 650 09-132792 (seal 000097)<br />

09KE8070 Blocked tank 37 09-132803 (seal 000163)<br />

09KE8071 Blocked tank with<br />

algae change<br />

18 09-465/44 (seal 000164)<br />

Comment: The report does not identify <strong>the</strong> matrix, presumably it is water. In some cases,<br />

<strong>the</strong>re appears to be two seal numbers <strong>for</strong> a single reported result.<br />

QHFSS Report 09KE8064-8071:SBC (Document 131 links to 220,223,224,225,221,222)<br />

Submitter: unstated (contact Roger Chong) Date sampled: unstated<br />

method:QIS16315<br />

Analysis: pesticide residues<br />

Lab No Descriptor Chemical Result (ug/L) Ref link<br />

09KE8064 Block tank Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

LORs: samples were tested <strong>for</strong> <strong>the</strong> following analytes.<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(ug/L)<br />

Analyte Limit <strong>of</strong> Reporting<br />

(ug/L)<br />

Methidathion 0.1 Dichlorvos 0.1<br />

B Cyfluthrin 0.1 Methoxyfenozide 0.001<br />

Carbendazim 0.01 2AB 1<br />

Comments: trichlorfon may have been analysed as dichlorvos (although not stated). Matrix<br />

analysed unstated but presumably water. 2AB not described but presumably is 2-<br />

aminobenzimidazole (metabolite <strong>of</strong> carbendazim). In some cases, <strong>the</strong>re appears to be two<br />

seal numbers <strong>for</strong> a single reported result.<br />

Sample 2<br />

BSL sample submission <strong>for</strong>m. (Document 223)<br />

Sample No: 09-132776<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Hatchery<br />

Date sampled: 3/11/09 9:30pm<br />

Date received: 11/11/09<br />

Sample: water<br />

History <strong>and</strong> clinical signs reported: spraying on <strong>the</strong> day <strong>of</strong> collection. Variable wind<br />

conditions.<br />

Analysis requested: Histology but also says <strong>for</strong> pesticide tests.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

Unstated (1) bottle water tap93 Unstated Pesticides<br />

Unstated (1) bottle water tap94 Unstated Pesticides<br />

Unstated Yellow belly fry 11/11/09 Fixed Histology<br />

Comments:<br />

It is not stated what containers were used or how all <strong>the</strong> samples were stored. No<br />

in<strong>for</strong>mation included on sampling methodology. No individual sample numbers.<br />

See QHFSS Report 09KN228-09KE8072:SC2 (Document 131) <strong>and</strong> QHFSS Report<br />

09KE8064-8071:SBC (Document 130) summarised above <strong>for</strong> analytical <strong>results</strong>.<br />

Sample 3<br />

BSL sample submission <strong>for</strong>m. (Document 224)<br />

Sample No: 09-132784<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Hatchery<br />

Date 28 May 2010 Page 101 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date sampled: 4/11/09 10:20pm<br />

Date received: 11/11/09<br />

Sample: water<br />

History <strong>and</strong> clinical signs reported: Spraying on <strong>the</strong> day <strong>of</strong> collection. Spraying on 4/11/09.<br />

Variable wind conditions.<br />

Analysis requested: pesticide tests<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

unstated Barra pond tag 95 unstated pesticides<br />

unstated Block tank tag 96 unstated pesticides<br />

Comments: It is not stated what containers were used or how all <strong>the</strong> samples were stored.<br />

No in<strong>for</strong>mation included on sampling methodology. No individual sample numbers.<br />

See QHFSS Report 09KN228-09KE8072:SC2 (Document 131) <strong>and</strong> QHFSS Report<br />

09KE8064-8071:SBC (Document 130) summarised above <strong>for</strong> analytical <strong>results</strong>.<br />

Sample 4<br />

BSL sample submission <strong>for</strong>m. (Document 211)<br />

Sample No: 08-180386.<br />

Submitter: Mat<strong>the</strong>w L<strong>and</strong>os<br />

Property: Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: Unstated<br />

Date received: 4/11/08<br />

Animal: Chicken<br />

History <strong>and</strong> clinical signs reported: de<strong>for</strong>med <strong>and</strong> dead on hatching. This year de<strong>for</strong>med <strong>and</strong><br />

unhatched eggs. Wing <strong>and</strong> leg de<strong>for</strong>mities. Unable to eat <strong>and</strong> drink. 2/8 affected; o<strong>the</strong>r 6 OK.<br />

30 eggs in incubator; none hatched. 15 eggs under hen –only 2 hatched. 1 died after 1-2<br />

hours. Also sudden onset resp distress ???? Owner concerned about spray drift.<br />

Analysis requested: Pesticides, biochemistry <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1897<br />

DP1898<br />

1 x liver Fresh frozen pesticides<br />

1 x kidney Fresh frozen Pesticides<br />

1 x gizzard Fresh frozen Pesticides<br />

1 x gizzard contents Fresh frozen Pesticides<br />

1 x small bowel intestine Fresh frozen Pesticides<br />

DP1899 Yolk/ liver Fresh frozen pesticides<br />

Date 28 May 2010 Page 102 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Chemical Residue Laboratory Report 6147 (Document 118)<br />

Submitter: Matt L<strong>and</strong>os Date sampled: 1/11/08 Method: AP016<br />

Lab No<br />

DP1897<br />

DP1898<br />

DP1899<br />

Sample<br />

descriptor<br />

Pooled liver/<br />

kidney bird 1<br />

Pooled GIT/ small<br />

intestine Bird 1<br />

Pooled liver/yolk<br />

Bird 2<br />

Chemical Result<br />

(mg/kg)<br />

DDE 0.08<br />

DDT 0.03<br />

Dieldrin 0.03<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Property: Sunl<strong>and</strong> Hatchery<br />

Date sampled: 5/11/09 10:30pm<br />

Date received: 11/11/09<br />

Sample: water <strong>and</strong> yellow belly <strong>fish</strong><br />

History <strong>and</strong> clinical signs reported: spraying on <strong>the</strong> day <strong>of</strong> collection. Spraying on 5/11/09.<br />

Variable wind conditions.<br />

Analysis requested: histology <strong>and</strong> pesticide<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

Unstated Barra pond tag 161 Unstated Pesticides<br />

Unstated Block tank tap 97 Unstated Pesticides<br />

Unstated Yellow belly Fixed Histology<br />

Comments:<br />

It is unstated what containers were used, how all <strong>the</strong> samples were stored. No in<strong>for</strong>mation<br />

included on sampling methodology. No individual sample numbers.<br />

See QHFSS Report 09KN228-09KE8072:SC2 (Document 131) <strong>and</strong> QHFSS Report<br />

09KE8064-8071:SBC (Document 130) summarised above <strong>for</strong> analytical <strong>results</strong>.<br />

Sample 6<br />

BSL sample submission <strong>for</strong>m. (Document 221)<br />

Sample No: 09-132803<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Hatchery<br />

Date sampled: 6/11/09 11:30pm<br />

Date received: 11/11/09<br />

Sample: water <strong>and</strong> yellow belly <strong>fish</strong><br />

History <strong>and</strong> clinical signs reported: spray????. Variable wind conditions<br />

Analysis requested: Histology but also says <strong>for</strong> pesticide tests (unclear).<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

Unstated Blocked tank water tag 163 Unstated Pesticides<br />

Unstated Yellow belly ??? fry 6/11/09 Unstated Histology?<br />

Unstated Yellow belly ??? fry 7/11/09 Unstated Histology?<br />

Comments:<br />

It is not stated what containers were used or how all <strong>the</strong> samples were stored. No<br />

in<strong>for</strong>mation included on sampling methodology. No individual sample numbers.<br />

Date 28 May 2010 Page 104 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

See QHFSS Report 09KN228-09KE8072:SC2 (Document 131) <strong>and</strong> QHFSS Report<br />

09KE8064-8071:SBC (Document 130) summarised above <strong>for</strong> analytical <strong>results</strong>.<br />

Sample 7<br />

BSL sample submission <strong>for</strong>m. (Document 222)<br />

Sample No: 09-132811<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Hatchery<br />

Date sampled: 8-11/11/09<br />

Date received: 11/11/09<br />

Sample: unstated<br />

History <strong>and</strong> clinical signs reported: 3-4 days after cessation <strong>of</strong> spraying. 10,000 fry stocked<br />

bara pond. Examined –most <strong>of</strong> <strong>the</strong> fry stocked not viable. Only 2 fry collected live.<br />

Analysis requested: Histology but also says <strong>for</strong> pesticide tests (unclear).<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

Unstated<br />

After spray 8/11/09 1 x bottle <strong>of</strong><br />

filter papers<br />

Unstated<br />

pesticides<br />

Unstated Yellow belly fry 10/11/09 Fixed Histology<br />

Unstated Yellow belly fry 11/11/09 Fixed Histology<br />

Comments:<br />

It is not stated what containers were used or how all <strong>the</strong> samples were stored. No<br />

in<strong>for</strong>mation included on sampling methodology. No individual sample numbers.<br />

Tank dimensions not known.<br />

QHFSS Report SSP0020561 09KE8072 (Document 132)<br />

Submitter: Roger Chong Date sampled: 11/11/09<br />

Method: sample extraction with dichloromethane using ultrasonic bath, followed by<br />

evaporation under vacuum <strong>and</strong> analysis by GCMS.<br />

Analysis: unstated<br />

Sample: Filter paper (23.5 cm diameter) reported to be 0.087m 2 by QHFSS.<br />

Lab No Descriptor Chemical Result (ug) Ref link<br />

09KE8072 Block tank Dichlorvos 0.055 09-132811<br />

Comments: The laboratory reports that ‘<strong>the</strong>y are unable to directly analyse trichlorfon, but is<br />

able to analyse dichlorvos which is a major decomposition product <strong>of</strong> trichlorfon’.<br />

Sample 8<br />

Date 28 May 2010 Page 105 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

BSL sample submission <strong>for</strong>m. (Document 203)<br />

Accession No: 09-133965.<br />

Submitter: Renee Thompson<br />

Property: QFNS0292 Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: 19/11/09<br />

Date received: 20/11/09.<br />

Animal: Duck<br />

History <strong>and</strong> clinical signs reported: Ducks brought onto property ~12 months ago. Have bred<br />

whilst on property. First lot <strong>of</strong> ducklings 7 survived from 13 eggs. Two broods since <strong>the</strong>n<br />

have not survived. Ei<strong>the</strong>r eggs turn putrid or ducklings are hatched still with large yoke sac<br />

but die quickly. The ducks were hatched on <strong>the</strong> farm.<br />

Analysis requested: Pesticides <strong>and</strong> biochemistry.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1980<br />

DP1981<br />

Entire duck – autopsy + clot tube<br />

(pre-mortem)<br />

Dead newly hatched duckling –<br />

autopsy<br />

Dead newly hatched duckling –<br />

autopsy<br />

Dead newly hatched duckling –<br />

autopsy<br />

unstated<br />

unstated<br />

unstated<br />

unstated<br />

Chemical Residue Laboratory Report 6622 (Document 121)<br />

Pesticides<br />

Pesticides<br />

Pesticides<br />

Pesticides<br />

Submitter: Renee Thompson Date sampled: 19/11/09 Method: AP016<br />

Lab No<br />

DP1980<br />

DP1981<br />

Sample<br />

descriptor<br />

Duck gastro<br />

intestinal tract<br />

(GIT)<br />

Pooled newly<br />

hatched ducklings<br />

Chemical Result<br />

(mg/kg)<br />

DDE 0.12<br />

DDD 0.06<br />

DDT 0.05<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

LORs <strong>of</strong> interest<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Methidathion 0.05 Trichlorfon 0.10<br />

Cyfluthrin 0.05 Cypermethrin 0.05<br />

p,p’ DDT 0.02 p,p’ DDE 0.02<br />

p,p’ DDD 0.02 Carbendazim 0.20<br />

Alpha endosulfan 0.02 Beta endosulfan 0.02<br />

Endosulfan sulfate 0.02 Chlorpyrifos 0.02<br />

Methoxyfenozide Not tested Fipronil Not tested<br />

Comments: Three ducklings were pooled to make DP1981. It is not stated what containers<br />

were used or how all <strong>the</strong> samples were stored. No in<strong>for</strong>mation included on sampling<br />

methodology.<br />

Sample 9<br />

BSL sample submission <strong>for</strong>m. (Document 229)<br />

Sample No: 09-136492<br />

Submitter: Renee Thompson<br />

Property: Sunl<strong>and</strong> Fish Hatchery (QFNS0292)<br />

Date sampled: unstated<br />

Date received: 18/12/09<br />

Sample: water<br />

History <strong>and</strong> clinical signs reported: water samples from ponds <strong>and</strong> tanks taken after spray<br />

events. Filter papers from inside hatchery shed.<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

Unstated 7 water samples (number in<br />

sheet attached<br />

Unstated Pesticides<br />

Attached Sheet (document 231)<br />

Number Description<br />

1 House tank (7/11/09)<br />

2 Barra pond (28/11/09)<br />

3 Block tank (25/11/09<br />

4 Barra Pond (25/11/09<br />

5 Block Tank (28/11/09)<br />

6 Filter paper from spraying 28/11/09 (collected<br />

14/12/09)<br />

7 House tank (29/11/09<br />

Date 28 May 2010 Page 107 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

QHFSS Laboratory Report 09KE8830-8830, 09KE8836:SBC (Document 137)<br />

Submitter: unstated Date sampled: unstated Method: 25391, EMN-009, QIS16315<br />

Lab No<br />

Sample<br />

Chemical<br />

Result<br />

Ref link<br />

Submission<br />

descriptor<br />

(ug/L)<br />

<strong>for</strong>m<br />

09KE8830<br />

House tank<br />

Urea (as N)


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

Sample<br />

Chemical<br />

Result<br />

Ref link<br />

Submission<br />

descriptor<br />

(ug/L)<br />

<strong>for</strong>m<br />

Beta<br />


SUMMARY OF RESULTS FOR NFHIT<br />

11/12/09<br />

S-7:30am<br />

F-12:00pm<br />

12/1/2010<br />

S-7:00am<br />

F- 3:30pm<br />

13/1/2010<br />

7:00am<br />

F-3:30pm<br />

14/1/2010<br />

7:00am<br />

F-3:30pm<br />

Glyphosate<br />

soyal phospholipids + propionic acid<br />

G6, G2, G5<br />

G2,Gh,G4<br />

Glyphosate<br />

soyal phospholipids + propionic acid<br />

G4,G3, G1, A2, A3 Glyphosate<br />

soyal phospholipids + propionic acid<br />

G1, A1, B1, B2, B3.1 Glyphosate<br />

soyal phospholipids + propionic acid<br />

Boom<br />

Boom<br />

Boom<br />

Boom<br />

15/1/2010<br />

B4, C1, C2, C3,<br />

Glyphosate<br />

Boom<br />

7:00am<br />

Knob, central, House<br />

soyal phospholipids + propionic acid<br />

F-3:30pm<br />

Wea<strong>the</strong>r records (Zeisemer)<br />

No records available.<br />

Samples<br />

Sample 1<br />

See sample submission 09-136492 (sample 9) in November 2009 above <strong>for</strong> details.<br />

QHFSS Report 09KE8835 (Document 143)<br />

Submitter: unstated Date sampled: 14/12/09<br />

Method: sample extraction with dichloromethane using ultrasonic bath, followed by<br />

evaporation under vacuum <strong>and</strong> analysis by GCMS.<br />

Analysis: unstated<br />

Sample: Filter paper (23.5 cm diameter) reported to be 0.043m 2 by QHFSS.<br />

Lab No Descriptor Chemical Result<br />

(ug/filter)<br />

09KE8835 Filter paper from Nonylphenol 0.140<br />

spraying Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Methidathion 0.06 Dichlorvos 0.150<br />

Cyfluthrin isomers 0.6 Cypermethrin Not tested<br />

Alpha endosulfan 0.300 Carbendazim 0.060<br />

Endosulfan sulfate 0.300 Beta endosulfan 0.300<br />

Nonylphenol (tech) 0.004 Nonylphenol 0.003<br />

2AB 1 O<strong>the</strong>r EDC?? 0.003<br />

O<strong>the</strong>r OPs?? 0.300 methoxyfenozide 0.060<br />

glyphosate 0.050 AMPA 0.050<br />

glufosinate 0.100 O<strong>the</strong>r OCs? 0.030<br />

Comments: The laboratory reports that ‘<strong>the</strong>y are unable to directly analyse trichlorfon, but is<br />

able to analyse dichlorvos which is a major decomposition product <strong>of</strong> trichlorfon’. Unclear<br />

what o<strong>the</strong>r OPs <strong>and</strong> EDCs were analysed. Ref link is different from submission to analytical<br />

report.<br />

Sample 2<br />

BSL sample submission <strong>for</strong>m. (Document 234)<br />

Sample No: 10-103510<br />

Submitter: Roger Chong<br />

Property: Sunl<strong>and</strong> Fish Hatchery<br />

Date sampled: unstated<br />

Date received: 12/2/10<br />

Sample: Silver perch<br />

History <strong>and</strong> clinical signs reported: mortalities in tanks in hatchery.<br />

Analysis requested: Pesticides <strong>and</strong> histology.<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

Unstated 1) Silver perch fry 4/1/10 Unstated Unstated<br />

Unstated 2) Fry silver perch 4/1/10 Frozen Unstated<br />

Unstated 3) S tank 1 Very cracked Unstated<br />

Unstated 4) Males pond mud Unstated Unstated<br />

Comments: samples 3 <strong>and</strong> 4 have ‘hold’ written against <strong>the</strong>m.<br />

QHFSS Report 10KE1115 (document 144) <strong>and</strong> amended QHFSS Report 10KE1115<br />

(document 147)<br />

Submitter: Roger Chong Date sampled: 4/1/10<br />

Method: 22276R2, lipid content by solvent extracted gravimetric analysis<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical Result (mg/kg) Ref link<br />

10KE1115 Sediment from DDE pp 0.06 10-103510-2<br />

Date 28 May 2010 Page 111 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

silver tank 1<br />

DDT pp 0.05<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

LORs <strong>of</strong> interest<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(ug/L)<br />

Analyte Limit <strong>of</strong> Reporting<br />

(ug/L)<br />

4-t-octylphenol 0.01 Nonylphenol 0.01<br />

Cholesterol (not an EDC) 0.01 Bisphenol A 0.01<br />

Glyphosate 0.5 AMPA 0.5<br />

Glufosinate 1.0<br />

QHFSS Report QHFSS Report 10KE1115-10KE1250: SBC2 (Document 153) <strong>and</strong> QHFSS<br />

Report QHFSS Report 10KE1115-10KE1250: SBC2 amended (document 154)<br />

Submitter: Roger Chong Date sampled: 12/2/10 Method: unstated<br />

lab No<br />

Sample<br />

Chemical<br />

Result<br />

Ref link<br />

Submission<br />

descriptor<br />

(ug/L)<br />

<strong>for</strong>m<br />

10KE1250<br />

Water<br />

Carbendazim


SUMMARY OF RESULTS FOR NFHIT<br />

Laboratory In<strong>for</strong>mation management (LIMS) templates. The link between <strong>the</strong> sample<br />

submission <strong>and</strong> report appears to be <strong>the</strong> package number SSP0021709.<br />

February 2010, event 10, Syndrome – Larval mal<strong>for</strong>mation, discoordination <strong>and</strong><br />

mortality<br />

Fish Health<br />

Noosa Fish Health Investigation Task Force, 6 th Veterinary Report – Silver Perch Larval<br />

Mal<strong>for</strong>mation, Discoordination <strong>and</strong> Acute Death Syndrome (document 609):<br />

Date<br />

Reported Event<br />

September 2009 No spraying observed after 27/9/09<br />

October 2009 No spraying observed<br />

November 2009 Spraying observed on 2, 3, 4, 5, 7, 11, 25, 26, 27 & 28<br />

December 2009 Spraying observed on 9 th<br />

1/12/09 Hatchery door left open<br />

9/12/09 Hatchery door shut<br />

17/12/09 New water from Ringtail ponds was delivered to top-up <strong>the</strong> water storages at <strong>the</strong><br />

hatchery.<br />

5/1/10 Hatchery door left open<br />

January 2010 Spraying on occasions<br />

1/1/10 Spawning <strong>of</strong> 3 groups <strong>of</strong> 4 Australian Bass<br />

3/1/10 Mortalities reported. 10,000 larvae survived from 711,000 fertilised embryos. The<br />

de<strong>for</strong>mity rate estimated was 25% <strong>of</strong> approximately 487,000 embryos. The<br />

swimming discoordination rate estimated was 65% <strong>of</strong> 380,100.<br />

Spray records<br />

Data taken from Chemical Application Record 2009 Spray Season – Arbuckles Farm<br />

(document 446) <strong>and</strong> chemical application record 2009 (document 447)<br />

Date/ time Blocks Actives Equipment Wea<strong>the</strong>r<br />

14/02/2010<br />

Glufosinate<br />

Boom<br />

E-NE winds<br />

S-6:00am<br />

G1, G3, G4, G6, A1,<br />

Soyal phospholipids + Propionic<br />

ligh to<br />

F-5:00pm<br />

B1<br />

acid<br />

variable<br />

15/2/2010<br />

Glufosinate<br />

Boom<br />

E/NE<br />

S-7:00am<br />

Soyal phospholipids + Propionic<br />

F-9:30am<br />

G5<br />

acid<br />

20/2/2010<br />

S-7:00<br />

F-9:50am<br />

A1, B1, C1<br />

Glufosinate<br />

Soyal phospholipids + Propionic<br />

acid<br />

Boom<br />

light S-SE<br />

Date 28 May 2010 Page 114 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

21/2/2010<br />

S-6:00am<br />

F-9:00am<br />

22/2/2010<br />

S-9:30am<br />

F-3:00pm<br />

B4, C1, B2<br />

G2, Gh, A3, B3,<br />

Knob, C3, C2<br />

Glufosinate<br />

Soyal phospholipids + Propionic<br />

acid<br />

Glufosinate<br />

Soyal phospholipids + Propionic<br />

acid<br />

Boom<br />

Boom<br />

light SW-SE<br />

E-SE<br />

Date/ time Blocks Fertiliser<br />

8/2/10 The lot Black urea<br />

February 2010 – OC Sniffer Dog<br />

BSL sample: 10-103533. (document 230)<br />

Submitter: Renee Thompson<br />

Property: Sunl<strong>and</strong> Fish Hatchery (QFNS0292)<br />

Date sampled: unstated<br />

Date received: 12/2/10<br />

Animal: soil<br />

DDT detection dogs located background levels- one spot @ fertiliser shed has (upwards<br />

arrow) levels.<br />

Analysis requested: Pesticides<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

DP1984 Fertiliser shed composite Unstated Pesticides<br />

DP1985 Car port drain Unstated Pesticides<br />

DP1986 Telstra/ house Unstated Pesticides<br />

DP1987 Gladioli shed Unstated Pesticides<br />

A map <strong>of</strong> sampling sites <strong>for</strong> sub 10-103533 (document 233) details <strong>the</strong> location <strong>of</strong> <strong>the</strong><br />

samples.<br />

Chemical Residue Laboratory Report 6662 (Document 140)<br />

Submitter: Renee Thompson Date sampled: 12/2/10 Method: AP016<br />

Lab No<br />

DP1984<br />

Sample<br />

descriptor<br />

Fertiliser shed<br />

composite<br />

Chemical Result<br />

(mg/kg)<br />

Chlorpyrifos 0.46<br />

Dieldrin 0.05<br />

ppDDE 0.19<br />

ppDDD 0.04<br />

opDDT 0.06<br />

Ref link Submission<br />

<strong>for</strong>m<br />

#10-103533 230<br />

Date 28 May 2010 Page 115 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

Sample<br />

Chemical<br />

Result<br />

Ref link<br />

Submission<br />

descriptor<br />

(mg/kg)<br />

<strong>for</strong>m<br />

ppDDT 0.37<br />

Moisture<br />

21 %w/w<br />

Multi residue<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Samples were analysed <strong>for</strong> a large number <strong>of</strong> Ops, Ocs <strong>and</strong> SPs. Reported on ‘as received’<br />

basis. LORs generally in <strong>the</strong> range: SPs 0.05, Ocs 0.02 <strong>and</strong> Ops 0.02-0.1 mg/kg.<br />

LORs <strong>of</strong> interest<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(mg/kg)<br />

Methidathion 0.05 Trichlorfon 0.10<br />

Cyfluthrin 0.02 Cypermethrin 0.05<br />

p,p’ DDT 0.01 p,p’ DDE 0.01<br />

p,p’ DDD 0.01 Carbendazim 0.20<br />

Alpha Endosulfan 0.01 Beta Endosulfan 0.01<br />

Endosulfan sulfate 0.01 Chlorpyrifos 0.02<br />

Methoxyfenozide Not tested Fipronil 0.01<br />

Comments: It is unstated what containers were used, how all <strong>the</strong> samples were stored. No<br />

in<strong>for</strong>mation included on sampling methodology. Sampling date in analytical report but not in<br />

sample submission <strong>for</strong>m.<br />

March 2010, event 11, Syndrome – Growth retardation<br />

Fish Heath<br />

Noosa Fish Health Investigation Task Force, 5 th Veterinary Report – Australian Bass Growth<br />

Retardation Syndrome (document 608):<br />

Date<br />

Reported Event<br />

16/8/09 Australian Bass hatched from Kurwongbah broodstock.<br />

September 2009 No spraying observed after 27/9/09<br />

October 2009 No spraying observed<br />

3/10/09 Stocked into <strong>the</strong> Barra pond at Gilson road with similarly aged cohorts from <strong>the</strong><br />

same spawning stocked at Ringtail. 110,000 bass <strong>of</strong> <strong>the</strong> single spawning, 50,000<br />

were stocked into <strong>the</strong> Ringtail pond White 1 – (RTW1) <strong>and</strong> 60,000 into Ringtail<br />

pond Red 4 <strong>for</strong> grow-out at 18 days old.<br />

November 2009 Spraying observed on 2,3, 4, 5, 7, 11, 25, 26,27 & 28<br />

18/11/09 600 bass from Red 4 pond were stocked into <strong>the</strong> Barra pond at 93 days old. The<br />

<strong>fish</strong> lengths <strong>of</strong> both Barra pond <strong>and</strong> RTW1 bass were observed to be similar<br />

being at 19 – 23 mm length at <strong>the</strong> start <strong>of</strong> <strong>the</strong> observation period commencing<br />

on 18/11/09 <strong>and</strong> finishing on 2/3/10 (RWT1) <strong>and</strong> 3/3/10 (Barra pond). Fish were<br />

r<strong>and</strong>omly selected <strong>for</strong> <strong>the</strong> stocking <strong>and</strong> sampling <strong>of</strong> <strong>the</strong> two ponds in <strong>the</strong><br />

observation period.<br />

November 2009 Fish were sampled from Ringtail <strong>for</strong> histology.<br />

December 2009 Fish were sampled from Barra pond <strong>for</strong> histology.<br />

December 2009 Spraying observed on 9 th<br />

January 2010 Spraying on occasions<br />

Date 28 May 2010 Page 117 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Date<br />

Reported Event<br />

February 2010 Spraying on occasions til 10 th<br />

March 2010 No spraying observed<br />

2/3/10 Fish were sampled from Ringtail <strong>for</strong> weight <strong>and</strong> length measurements.<br />

Sampling was conducted by routine netting <strong>and</strong> <strong>fish</strong> were r<strong>and</strong>omly sampled<br />

from <strong>the</strong> nets.<br />

3/3/10 Fish were sampled from Barra pond <strong>for</strong> weight <strong>and</strong> length measurements.<br />

Sampling was conducted by routine netting <strong>and</strong> <strong>fish</strong> were r<strong>and</strong>omly sampled<br />

from <strong>the</strong> nets.<br />

Spray Records<br />

Data taken from Chemical Application Record 2009 Spray Season – Arbuckles Farm<br />

(document 446) <strong>and</strong> chemical application record 2009 (document 447)<br />

No applications recorded <strong>for</strong> March.<br />

ENVIRONMENTAL DATA<br />

Evaluation <strong>of</strong> Organic Chemical Contaminants in <strong>the</strong> Cooloothin Creek Using PDMS <strong>and</strong> ED<br />

Passive (document 133) <strong>and</strong> Detection <strong>and</strong> Measurement <strong>of</strong> Pesticide Concentrations in<br />

Waters <strong>of</strong> <strong>the</strong> Noosa River Catchment by Queensl<strong>and</strong> EPA/DERM 2009-2010 (document<br />

155)<br />

Date <strong>of</strong> placement: 22/1/09 – 19/2/09<br />

Sampler: EPA staff (no individual specified)<br />

Method: PDMS strip passive samplers <strong>and</strong> EmporeTM Disk (SDB Disk Chemcatchers) in <strong>the</strong><br />

linear kinetic phase <strong>of</strong> uptake on <strong>the</strong> Empore TM Disk. GCMS instrumental analysis.<br />

Placement: Cooloothin Creek which flows into Lake Cootharaba: Upstream, Downstream 1<br />

<strong>and</strong> Downstream 2. Replicate samplers deployed at Downstream 1, whereas single<br />

samplers were deployed at <strong>the</strong> o<strong>the</strong>r 2 sites.<br />

Lab No<br />

unstated<br />

unstated<br />

Sample<br />

descriptor<br />

Upstream<br />

Downstream1A<br />

Chemical Result (ug/L) Ref link<br />

Galaxolide 0.0026<br />

unstated<br />

TCPP 0.0576<br />

Tonalide 0.00056<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

unstated<br />

Sample<br />

descriptor<br />

Downstream1B<br />

unstated Downstream 2<br />

Chemical Result (ug/L) Ref link<br />

Metolachlor 0.01475<br />

Atrazine 0.00965<br />

TCPP 0.0306<br />

Galaxolide 0.001<br />

Tonalide 0.0003<br />

Carbendazim 0.0004 – 0.001<br />

Multi residue<br />

(o<strong>the</strong>rs)<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Sampler: EPA staff (no individual specified)<br />

Method: PDMS strip passive samplers <strong>and</strong> EmporeTM Disk (SDB Disk Chemcatchers) in <strong>the</strong><br />

linear kinetic phase <strong>of</strong> uptake on <strong>the</strong> Empore TM Disk. GCMS instrumental analysis.<br />

Placement: Cooloothin Creek which flows into Lake Cootharaba: Upstream, Downstream 1<br />

<strong>and</strong> Downstream 2. Replicate samplers deployed at Downstream 1, whereas single<br />

samplers were deployed at <strong>the</strong> o<strong>the</strong>r 2 sites.<br />

Lab No<br />

Sample<br />

Chemical Result (ug/L) Ref link<br />

descriptor<br />

Unstated Blank Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

Sample<br />

descriptor<br />

Unstated Site 8<br />

unstated Site 9<br />

Chemical Result (ug/L) Ref link<br />

sulfate<br />

Carbendazim 0.0007<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Samples submitted:<br />

Lab Ref Description Condition/type Analysis Req<br />

CUC1060 Water sample from Lake<br />

Cooroibah<br />

unstated<br />

OPs including methidathion<br />

Chemical Residue Laboratory Report 6074 original <strong>and</strong> amended (Documents 119 & 120)<br />

Submitter: Ian Walker Date sampled: 23/9/08 Method: FV005 modified<br />

Lab No<br />

CUC1060<br />

Sample<br />

descriptor<br />

Water from Lake<br />

Cooroibah<br />

Chemical Result Ref link Submission<br />

(mg/kg)<br />

<strong>for</strong>m<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Comments:<br />

It states hold in freezer. It is unclear <strong>of</strong> <strong>the</strong>y were intended to be analysed. No sample<br />

numbers. Unclear if <strong>the</strong> flesh, liver <strong>and</strong> gonads are combined or separate.<br />

QHFSS Report 09KE1277-1279 (document 142)<br />

Submitter: unstated Date sampled: 22/07/09<br />

Method: 22276R2, lipid content by solvent extracted gravimetric analysis<br />

QHFSS lab No<br />

10KE1277<br />

10KE1329<br />

10KE1330<br />

Sample<br />

Chemical Result (mg/kg) Ref link<br />

descriptor<br />

Noosa river <strong>fish</strong> Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

Sample<br />

Chemical Result (ug/L) Ref link<br />

descriptor<br />

Unstated Site 1<br />

Diuron 0.0045<br />

Unstated<br />

Carbendazim 0.0003<br />

TCPP 0.044<br />

Multi residue<br />

(o<strong>the</strong>r)<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Lab No<br />

Sample<br />

descriptor<br />

Chemical Result (ug/L) Ref link<br />

Carbendazim 0.0001<br />

TCPP 0.043<br />

Galaxolide 0.0009<br />

Multi residue<br />

(o<strong>the</strong>r)<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(ug/L)<br />

Analyte<br />

Limit <strong>of</strong> Reporting<br />

(ug/L)<br />

Methidathion 0.1 Dichlorvos 0.1<br />

Cyfluthrin 0.1 Cypermethrin Not tested<br />

p,p’ DDT 0.1 p,p’ DDE 0.05<br />

p,p’ DDD 0.1 Carbendazim Not tested<br />

Alpha endosulfan 0.01 Beta endosulfan 0.01<br />

Endosulfan sulfate 0.01<br />

Comments:<br />

This report may be outside <strong>the</strong> TORs based on date received <strong>of</strong> 14/4/04. It also appears that<br />

a purge <strong>and</strong> trap volatile method was also used <strong>and</strong> no xylene was detected. The purge <strong>and</strong><br />

trap <strong>results</strong> do not appear to be present in <strong>the</strong> report. Not stated but trichlorfon maybe<br />

analysed as dichlorvos.<br />

(Second report in Document 109)<br />

Submitter: Paul Murray<br />

Property: unstated<br />

Date sampled: unstated<br />

Date received: 13/11/09<br />

Water <strong>for</strong> human consumption – property unstated<br />

Test method: SHW17<br />

Lab No<br />

Sample<br />

descriptor<br />

analyte<br />

Result<br />

(CFU/ml)<br />

Ref link<br />

Submission<br />

<strong>for</strong>m<br />

09 13574 Unstated<br />

09 13574 Unstated<br />

09 13575 Unstated<br />

Coli<strong>for</strong>ms MPN<br />

per 100 ml<br />

E coli: MPN per<br />

100 ml<br />

Coli<strong>for</strong>ms MPN<br />

per 100 ml<br />

E coli: MPN per<br />

100 ml<br />

Coli<strong>for</strong>ms MPN<br />

per 100 ml<br />

E coli: MPN per<br />

100 ml<br />

190<br />

1<br />

310<br />

0<br />

10<br />

0<br />

6120N (A)<br />

6120N (B)<br />

6161N<br />

Not<br />

provided<br />

Not<br />

provided<br />

Not<br />

provided<br />

Comments:<br />

The report indicates that sample 09 13574 did not meet <strong>the</strong> microbiological requirements <strong>of</strong><br />

<strong>the</strong> NH&MRC drinking water guidelines. Although <strong>the</strong> samples (residue <strong>and</strong> microbiological)<br />

were provided by Q<strong>health</strong> to <strong>the</strong> Task<strong>for</strong>ce at <strong>the</strong> same time, <strong>the</strong> samples do not relate to<br />

<strong>the</strong> same time period. It is unclear if <strong>the</strong>y relate to <strong>the</strong> same property.<br />

Date 28 May 2010 Page 126 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

Sample 3<br />

Queensl<strong>and</strong> Health FSSe Report 09KE7903-09KE7910:SC2 (Document 122)<br />

Submitter: Environmental Health – Maroochydore (Paul Murray?) Date sampled: 12/11/09<br />

Method: QIS16315<br />

Lab No<br />

09KE7903<br />

09KE7904<br />

09KE7905<br />

09KE7906<br />

09KE7907<br />

09KE7908<br />

09KE7909<br />

09KE7910<br />

Sample<br />

descriptor<br />

Unstated<br />

Unstated<br />

Unstated<br />

Unstated<br />

Unstated<br />

Unstated<br />

Unstated<br />

Unstated<br />

Chemical Result (ug/L) Ref link<br />

Methoxyfenozide 0.002<br />

6112N<br />

Multi residue


SUMMARY OF RESULTS FOR NFHIT<br />

(ug/L)<br />

(ug/L)<br />

Methidathion 0.1 Dichlorvos 0.1<br />

Cyfluthrin 0.1 Cypermethrin 0.1<br />

p,p’ DDT 0.1 p,p’ DDE 0.1<br />

p,p’ DDD 0.1 Carbendazim 0.01<br />

2-aminobenzamidazole 1 Beta Endosulfan 0.05<br />

Alpha Endosulfan 0.05 Methoxyfenozide 0.001<br />

Endosulfan sulfate 0.05 Glufosinate 1<br />

Nonylphenol 0.001 Glyphosate 0.5<br />

AMPA 0.5<br />

Comments: The report does not state what matrix was analysed or provide a sample<br />

descriptor. However, it is suspected that <strong>the</strong>se are <strong>results</strong> <strong>for</strong> tank water.<br />

Reports states that <strong>the</strong> samples complied with <strong>the</strong> Australian Drinking Water Guidelines<br />

2004. Dichlorvos may include trichlorfon, but not stated.<br />

Sample 4<br />

QHFSS Report 09KE8431-09KE8447: SC2 (Document 126)<br />

Submitter: Environmental Health – Maroochydore Date sampled: 7/12/09<br />

Method: QIS16315<br />

Sample: water<br />

Lab No<br />

Sample Chemical Result (ug/L) Ref link<br />

descriptor<br />

09KE8431 Unstated nonylphenol 0.170 Unstated<br />

Multi residue<br />


SUMMARY OF RESULTS FOR NFHIT<br />

Methoxyfenozide 0.001<br />

Multi residue<br />


SUMMARY OF RESULTS FOR NFHIT<br />

DOCUMENTS LIST<br />

Protocols <strong>and</strong> Plans<br />

No.<br />

Document<br />

1 Sampling plan <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation Task<strong>for</strong>ce ver 5, 4 September 2009<br />

2 Sampling plan <strong>for</strong> <strong>the</strong> Noosa Fish Health Investigation Task<strong>for</strong>ce ver 5a<br />

3 A Protocol <strong>for</strong> Responding to Adverse Fish Health Events <strong>and</strong> Chemical Overspray/Drift<br />

Incidents at Sunl<strong>and</strong> Fish Hatchery<br />

4 Sunl<strong>and</strong> Fish Hatchery Veterinary Investigation Work Instruction<br />

Analytical reports<br />

No. Document Sample Nos Sample<br />

date<br />

Relates to<br />

100 Chemical Residue Laboratory Report 6252 DP1914R-<br />

DP1916R,<br />

22/12/2008 #08-184980, 08-<br />

185511<br />

DP1918R<br />

101 Chemical Residue Laboratory Report 6253 DP1926- 8/12/2008 09-103042<br />

DP1929<br />

102 Chemical Residue Laboratory Report 6254 DP1930- 4/02/2009 #09-103042<br />

DP1931<br />

103 Chemical Residue Laboratory Report 6255 DP1932- 11/02/2009 #09-103692<br />

DP1933<br />

104 Chemical Residue Laboratory Report 6256 DP1934 12/02/2009 #09-103703<br />

105 Chemical Residue Laboratory Report 6134 DP1893- 16/10/2008 #08-178975<br />

DP1894<br />

106 Chemical Residue Laboratory Report 6197 CUC1086 8/12/2008 #080739<br />

107 Chemical Residue Laboratory Report 6196 CUC1085 8/12/2008 #080738<br />

108 Chemical Residue Laboratory Report 6196 CUC1084 8/12/2008 #080737<br />

109 Queensl<strong>and</strong> Health Report 03EP5070 5070, 09 unknown unknown<br />

replacement report<br />

13574- 09<br />

13575<br />

110 Chemical Residue Laboratory Report 6203 DP1917 16/12/2008 #08-184972<br />

111 Chemical Residue Laboratory Report 6200 DP1908 9/12/2008 #08-179890<br />

112 Chemical Residue Laboratory Report 6198 DP1907 16/12/2008 #08-179890<br />

113 Chemical Residue Laboratory Report 6202 DP1914- 16/12/2008 #08-184980<br />

DP1915<br />

114 Chemical Residue Laboratory Report 6201 DP1910- 9/12/2008 #08-182660<br />

DP1912<br />

115 Chemical Residue Laboratory Report 6199 DP1907 9/12/2008 #08-182660<br />

116 Chemical Residue Laboratory Report 6148 DP1900-<br />

DP1901<br />

1/11/2008 #08-180371<br />

Date 28 May 2010 Page 130 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

No. Document Sample Nos Sample<br />

date<br />

Relates to<br />

117 Chemical Residue Laboratory Report 6134 DP1893- 16/10/2008 #08-178975<br />

(amended)<br />

DP1894<br />

118 Chemical Residue Laboratory Report 6147 DP1897- 1/11/2008 #08-180386<br />

DP1899<br />

119 Chemical Residue Laboratory Report 6074 CUC1060 23/09/2008 08.0794<br />

amended<br />

120 Chemical Residue Laboratory Report 6074 CUC1060 23/09/2008 08-0794<br />

121 Chemical Residue Laboratory Report 6622 DP1980- 19/11/2009 #09-133965<br />

DP1981<br />

122 Queensl<strong>and</strong> Health FSSe Report<br />

09KE7903- 12/11/2009 6112N-6119N<br />

09KE7903-09KE7910:SC2<br />

09KE7910<br />

123 Chemical Residue Laboratory Report 6204 DP1918 12/12/2008 #08-185511<br />

124 EnTox passive sampler report unknown 27/7/08- unknown<br />

26/8/08<br />

125 QHFSS Report 09EN3982/3985 :GP EN3982-<br />

EN3984<br />

20/10/2006 JMN201009A07,<br />

JMN201009A08,<br />

JMN201009C07,<br />

JMN201009C08<br />

126 QHFSS Report 09KE8431-09KE8447:SC2 6156N-<br />

6171N<br />

127 QHFSS Report 09KE6807-09KE6809:<br />

PW1<br />

128 QHFSS Report 09NA-12984-<br />

09NA12985:TKP2<br />

09NA12984,<br />

09NA12985<br />

23/09/2009 JMN230909A01_6,J<br />

MN230909C01_6<br />

129 Water readings <strong>for</strong> Sunl<strong>and</strong> Fish Hatchery<br />

23-9-09<br />

portable<br />

meter<br />

readings<br />

130 QHFSS Report 09KE8064-8071:SBC 09KE8064-<br />

09KE8071<br />

unstated<br />

but<br />

received<br />

19/11/09<br />

09-132761, 09-<br />

132776, 09-132784,<br />

09-132792, 09-<br />

132803, 09-465/44<br />

131 QHFSS Report 09KN228-09KE8072:SC2 09KE8064-<br />

09KE8072<br />

3/11/2009 09-132761, 09-<br />

132776, 09-132784,<br />

09-132792, 09-<br />

132803, 09-132811<br />

132 QHFSS Report SSP0020561 09KE8072 09KE8072 11/11/2009 09-132811<br />

133 Evaluation <strong>of</strong> Organic Chemical<br />

22/1/09- 133<br />

Contaminants in <strong>the</strong> Cooloothin Creek<br />

Using PDMS <strong>and</strong> ED Passive<br />

19/2/09<br />

134 Comparison <strong>of</strong> DERM passive sampling dec 09-Jan10 134<br />

<strong>results</strong> from dry season/ macadamia no<br />

spray season<br />

135 QHFSS Report 08NA12582- 10/12/2008 18-182660, 08-<br />

Date 28 May 2010 Page 131 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

No. Document Sample Nos Sample<br />

date<br />

Relates to<br />

08NA12583XY 179890<br />

136 QHFSS Report 08PE328-330 UT unstated 18-182660, 08-<br />

179890<br />

137 QHFSS Report 09KE8830-8834,<br />

unstated unstated<br />

KE8836:SBC<br />

138 Passive sampler Noosa Catchment Report<br />

October 2009 _FINAL<br />

27/7/09-<br />

26/8/09<br />

139 passive sampler 3 rd Noosa Catchment<br />

Report February 2010_FINAL<br />

11/12/09-<br />

11/1/10<br />

140 Chemical Residue Laboratory Report 6662 DP1984- 12/02/2010<br />

DP1987<br />

141 QHFSS Report 09KE1258-1264, 1328- 09KE1258- unstated 09-120470<br />

1330<br />

1264,<br />

KE1328-1330<br />

142 QHFSS report 10KE1277-1279 0KE1277- 22/07/2009<br />

1279<br />

143 QHFSS Report 09KE8835 09KE8835 14/12/2009 09-136492<br />

144 QHFSS Report 10KE1115 10KE1115 4/01/2010 10-103510<br />

145 QHFSS Report 09KE7314 09KE7314 20/10/2009 JMN201009A09<br />

146 QHFSS Report 10KE1061 10KE1061 9/02/2010 JMN090210F01<br />

147 amended QHFSS Report 10KE1115 10KE1115 4/01/2010 10-103510<br />

148 QHFSS Report 09KE6901-6908 09KE6901-<br />

6908<br />

23/09/2009 JMN230909A05-<br />

JMN230909A06,<br />

JMN230909C04-<br />

JMN230909C05,<br />

JMN230909B01-<br />

JMN230909B02,<br />

JMN230909H01-<br />

JMN230909H02<br />

149 QHFSS Report 09KE6902-6906 Amended 09KE6902-<br />

6906<br />

23/09/2009 JMN230909A05-<br />

JMN230909A06,<br />

JMN230909C04-<br />

JMN230909C05,<br />

JMN230909B01-<br />

JMN230909B02,<br />

JMN230909H01-<br />

JMN230909H02<br />

150 QHFSS Report 09KE6901-09KE6908: pw1 09KE6901- 23/09/2009 JM230909 water<br />

6908<br />

151 QHFSS Report 09KE1250: SBC 09KE1250 unstated, 10-103510<br />

rec’d<br />

15/2/10<br />

152 09KE8064-09KE8071 09KE8064- unstated 09-132761, 09-<br />

Date 28 May 2010 Page 132 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

No. Document Sample Nos Sample<br />

date<br />

Relates to<br />

153 QHFSS Report 10KE1115-10KE1250:<br />

SBC2<br />

09KE8071<br />

10KE1250<br />

154 QHFSS Report 10KE1115-10KE1250:<br />

SBC2 amended<br />

but 132776, 09-132784,<br />

received 09-132792, 09-<br />

19/11/09 132803, 09-465/44<br />

12/2/10 unstated<br />

10KE1115-<br />

10KE1115-<br />

10KE1251 12/02/2010 unstated<br />

155 Detection <strong>and</strong> Measurement <strong>of</strong> Pesticide Concentrations in Waters <strong>of</strong> <strong>the</strong> Noosa River Catchment<br />

by Queensl<strong>and</strong> EPA/DERM 2009-2010<br />

156 RE:<br />

QPIF_EDOCS_n44329_v1_D138_Passive_sampler_Noos<br />

a_Catchment_Report_October_2009__FINAL.pdf - Adobe<br />

Reader<br />

email<br />

Munro<br />

Mortimer to<br />

Richard<br />

Watts<br />

Fri 28/05/2010 10:26<br />

AM<br />

Sample submissions<br />

No Document Lab no Date submitted<br />

200 08-180371 DP1900-DP1902 unknown<br />

201 08-179890 DP1907-DP1908 9/12/2008<br />

202 08-178975. unknown 17/10/2008<br />

203 09-133965. DP1980-DP1981 19/11/2009<br />

204 09-103703. DP1934 11/02/2009<br />

205 09-103692. DP1932-DP1933 11/02/2009<br />

206 09-103042. DP1926-DP1931 8/12/2008<br />

207 08-185511. DP1918 21/12/2008<br />

208 08-184980. unstated 10/12/2008<br />

209 08-182660. DP1910-DP1912, 9/12/2008<br />

unstated<br />

210 08-184972 DP1910 10/12/2008<br />

211 08-180386. DP1897-DP1899 unknown<br />

212 08.0739 CUC1086 10/12/2008<br />

213 08.0738 CUC1085 10/12/2008<br />

214 08.0737 CUC1084 10/12/2008<br />

215 09-121094, 09-120470. unstated 22/7/069, 27/07/2009<br />

216 Compilation <strong>of</strong> documents received from<br />

Hodge<br />

217 QHFSS sample submission <strong>for</strong>m <strong>for</strong><br />

JMN090210F01<br />

Date 28 May 2010 Page 133 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

218 QHFSS sample submission <strong>for</strong>m <strong>for</strong><br />

JMN230909<br />

219 QHFSS sample submission <strong>for</strong>m <strong>for</strong> JMN201009A07- 219<br />

JMN201009<br />

230909A08,<br />

JMN201009C07-<br />

230909C08,<br />

JMN230909C01-<br />

JMN230909C06,<br />

JMN230909H01-<br />

JMN230909H02,<br />

JMN230909D01-<br />

JMN230909D03,<br />

JMN201009A09<br />

220 09-132761 unstated 3/11/2009<br />

221 09-132803 unstated 6/11/2009<br />

222 09-132811 unstated 8-11/11/09<br />

223 09-132776 unstated 3/11/2009<br />

224 09-132784 unstated 4/11/2009<br />

225 09-132792 unstated 5/11/2009<br />

226 08.0794 cuc1060 23/09/2008<br />

227 sample plan 23-9-09 JMN230909A01- 22/1/09-19/2/09<br />

JMN230909A06,<br />

JMN230909C01-<br />

JMN230909C06,<br />

JMN230909B01,<br />

JMN230909D01,<br />

JMN230909H01-<br />

JMN230909H02<br />

228 09-126853 unstated unstated, submitted<br />

18/9/09<br />

229 09-136492 unstated unstated, submitted<br />

18/12/09<br />

230 10-103533 DP1984-DP1987 Unstated, submitted<br />

12/2/10<br />

231 09-136492 attachment<br />

232 09-126853 attachment<br />

233 map <strong>of</strong> sampling sites <strong>for</strong> sub10-103533<br />

234 10-103510 unstated unstated 4/1/10?<br />

O<strong>the</strong>r documents<br />

No Document<br />

300 Timeline <strong>of</strong> events at Sunl<strong>and</strong> Fish Hatchery FINAL 12 MAY 2009.doc<br />

400 The nut farm, owner R. Arbuckle<br />

401 Chemical application record, Jondell (Arbuckles)<br />

Date 28 May 2010 Page 134 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

402 Untitled document (wea<strong>the</strong>r conditions 4-10-08-9-12-08)<br />

403 SPRAYING RECORDS - ARBUCKLE PROPERTY, 30/07/2007 TO 05/11/2007<br />

404 REPORT _SUNLAND FISH HATCHERY_IW_290409.doc<br />

405 SUPPLEMENTARY_REPORT _SUNLAND FISH HATCHERY_IW_220509.doc<br />

406 Appendix 1-Diagram <strong>of</strong> Macadamia Farm.doc<br />

407 Appendix 2-Property Map ex APS.doc<br />

408 Appendix 3_Diagram_Pond layout.doc<br />

409 Appendix 4_Photograph_Gilson 2006.doc<br />

410 Appendix 5-Map <strong>of</strong> Gilson property- Pond Location.doc<br />

411 Appendix 6_Record <strong>of</strong> Sample Taken Males Pond 8-12-08.pdf<br />

412 Appendix 6_Water sample -Males Pond.doc<br />

413 Appendix 7_Record <strong>of</strong> Sample Taken Barra Pond 8-12-08.pdf<br />

414 Appendix 7_Water sample_Barra Pond.doc<br />

415 Appendix 8_Record <strong>of</strong> Sample Taken Spawning Tank 8-12-08.pdf<br />

416 Appendix 8_Water sample_spawning shed.doc<br />

417 Appendix 9_Gilsons Males Pond Water Sample Results.pdf<br />

418 APPENDIX 9_Lab report water -Males Pond.doc<br />

419 Appendix 10_Lab report water -Barra pond.doc<br />

420 Appendix 10-Gilsons Barra Pond Water Results.pdf<br />

421 Appendix 11_ Gilsons Spawning Tank Water Results 14-1-09.pdf<br />

422 Appendix 11_Lab report -Spawning Tank.doc<br />

423 Appendix 13_Spray Records 2005-2006.doc<br />

424 Appendix 14_Spray Records 2007.doc<br />

425 Appendix 15-Waypoint spraying observation.doc<br />

426 Appendix 16_Spray Records 2008.doc<br />

427 Appendix 17_Spray Records_Wea<strong>the</strong>r Conditions 2008-2009 season.doc<br />

428 Appendix 18_ Spray Records Herbicide Applications 2008.doc<br />

429 Appendix 19_Buffer Area locations_ Ziesemer.doc<br />

430 Appendix 20_Record <strong>of</strong> Sample Taken Lake Cooroibah 23-9-08.pdf<br />

431 APPENDIX 20-Water Sample_Lake Cooroibah.doc<br />

432 Appendix 21_ Lab Report_Water LakeCooroibah.doc<br />

433 Appendix 21_Lake Cooroibah Sample CUC1060.pdf<br />

434 Appendix 22_Wea<strong>the</strong>r_Tewantin Oct08.pdf<br />

435 Appendix 23_Wea<strong>the</strong>r_Tewantin Nov08.pdf<br />

436 Appendix 24_Wea<strong>the</strong>r_Tewantin Dec08.pdf<br />

437 spray times 31-8-09 - 28-11-09<br />

438 Distance estimates – 6/9/2009 Chemical Applications<br />

439 intention to spray notice 29-9-06<br />

440 intention to spray notice 9-10-06<br />

431 APPENDIX 20-Water Sample_Lake Cooroibah.doc<br />

441 intention to spray notice 6-11-06<br />

442 intention to spray notice 22-10-07<br />

443 intention to spray notice 18-11-08<br />

444 Atrosine <strong>for</strong>te label<br />

Date 28 May 2010 Page 135 <strong>of</strong> 136


SUMMARY OF RESULTS FOR NFHIT<br />

445 Pomona <strong>fish</strong> hatchery windroses<br />

446 Ziesemer Pesticide_herbicide records 09<br />

447 chemical application record 2009<br />

448 Report on Sunl<strong>and</strong> Hatchery - Chemicals Used on Hatchery<br />

449 Appendix 1 Hatchery Chemicals<br />

450 Draft Sunl<strong>and</strong> Inspection_Chem Use - Supplement Rpt<br />

451 POMONA_met_12Oct-04Feb10<br />

500 Noosa Fish Health Investigation Task<strong>for</strong>ce Interim Report #1, June 2009<br />

501 Noosa Fish Health Investigation Task<strong>for</strong>ce Interim Report #2, February 2010<br />

Noosa Fish Health Investigation Task Force, 3rd Veterinary Report, Bass Fry Kill Syndrome 1st<br />

600 Ed, undated<br />

Noosa Fish Health Investigation Task Force, 2nd Veterinary Report, Mullet De<strong>for</strong>mities<br />

601 Syndrome, 29-9-09<br />

602 AER, L<strong>and</strong>os<br />

603 Vet records O Johnson<br />

Noosa Fish Health Investigation Task Force, 1st Veterinary Report on Sunl<strong>and</strong> Fish Hatchery,<br />

604 For Scientific Subcommitee, 20/3/09<br />

605 Record <strong>of</strong> visit <strong>and</strong> sampling - Sunl<strong>and</strong> Fish Hatchery 16-12-08<br />

606 Sunl<strong>and</strong> Fish Hatchery - Farm inspection <strong>and</strong> Sampling 9.12.08 Report 15.12.08<br />

Noosa Fish Health Investigation Task Force, 4th Veterinary Report, Golden Perch Loss<br />

607 Syndrome<br />

Noosa Fish Health Investigation Task Force, 5th Veterinary Report, Australian BAss growth<br />

608 retardation Syndrome<br />

Noosa Fish Health Investigation Task Force, 6th veterinary Report, Silver Perch Larval<br />

609 Mal<strong>for</strong>mation, Discoordination <strong>and</strong> Acute Death Syndrome<br />

610 Memo, Renee Thompson, 18-2-10<br />

611 untitled attachment 1 to Memo, Renee Thompson, 18-2-10<br />

612 attachment 2 to Memo, Renee Thompson, 18-2-10<br />

613 attachment 3 to Memo, Renee Thompson, 18-2-10<br />

614 attachment 4 to Memo, Renee Thompson, 18-2-10<br />

615 attachment 5 to Memo, Renee Thompson, 18-2-10<br />

Date 28 May 2010 Page 136 <strong>of</strong> 136

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