Tecnai™ G2 Spirit Product Brochure - FEI Company

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2 Meeting the needs of the scientific community Fundamental research within the scope of cell biology, structural biology, soft matter and nano- technology is increasingly focusing on understanding interactive biological processes, pathways and structures at the macromolecular level. Transmission electron microcopy (TEM) is of paramount importance to obtain high-magnification and high-resolution 2D and 3D information of cells and organelles or even smaller cell constituents. A pre-requisite for optimal 2D and 3D-tomographic imaging is a high level of microscope automation and intelligence. The new Tecnai G 2 Spirit – built on more than 50 years’ experience in Life Science – combines modern day technology to satisfy the stringent demands of both today’s and tomorrow’s scientific community. The Tecnai G 2 Spirit ensures high-quality images, is easy to use and is designed to facilitate the work of the microscope user. It has a high level of automation, yet doesn't limit full control for experienced expert users. The microscope is ready for novel applications like 3D-imaging using tomography and/or cryo-electron microscopy. The Spirit’s design philosophy ensures application flexibility for today and tomorrow, it holds a ticket to the future: functionality can be added or upgraded by uploading current and new application software. In the world of Life Sciences, developments occur at a high pace. FEI Company has ongoing collaborations with the relevant scientific communities to better understand their needs and co-develop dedicated tools. The Tecnai G 2 Spirit is building a further and more detailed understanding in the world of decreasing dimensions: today and in the future. 3
4 Figure 1: Diagnostic Research Lung biopsy from a patient suffering from SARS. Image courtesy of: Queen Elisabeth II Hospital Hong Kong. Figure 2: Cell Biology Research Immunogold labeling of plasmodesmata in genetically modified plants Diagnostic research As a consequence of the growing and aging world population, the number of patients needing advanced medical care and treatment inevitably increases. This stresses the urgency for fast, reliable screening of disease patterns. As a mainstream diagnostic modality in medical centers, the Tecnai G2 Spirit is a ready-to-use system that ensures a high level of automation and a high sample throughput to cope with the need for increasing the speed of diagnostics. Furthermore, the full digital Tecnai G2 Spirit allows for on-line consultation with colleagues and experts in the field to enable a fast and correct diagnosis. Cell Biology research Achieving breakthroughs that can improve human health or increase the quality or yield of agricultural products requires research to the underlying fundamental processes. For studying the functionality of ongoing physiological and pathological pathways, the Tecnai G2 Spirit is perfectly suited. For retrieval of cell biological data, the Spirit is set for fast and (semi-)automated 2D and 3D high-quality image acquisition and analysis. Its ease-of-use makes the instrument very attractive for multiuser scientific environments like universities and institutes. Pharmaceutics and Chemistry The Tecnai G2 Spirit is an effective and efficient tool to gain insight into release mechanisms of drugs in the body. In addition, the effects of medicines on the morphology and functioning of tissues and organs need to be investigated. The Spirit, optimized for 2D and 3D imaging of cells, cell organelles and soft matter combined with a high level of automation can play an essential role in this and is of significant importance for continued advances in healthcare and chemistry. Figure 3: Pharmaceutics and Chemistry Cryo TEM image of cis-Pt particles packaged in PS liposomes. The liposomes are used as novel drug targeting entities in anti-cancer research. Image courtesy of Dr. P. Frederik, University of Maastricht, The Netherlands. High Image quality – throughout the entire magnification range All biological TEM specimens, ranging from ultra-thin sections to negative stained samples, have in common that for high-quality visualization maximized contrast and resolution are required. In general, the imaging magnifications are in the intermediate range, roughly from about 500 to 100 000 times covering 95% of the used applications. The optics of the Tecnai G2 Spirit – with the well proven and patented BioTWIN and TWIN concept – provides sharp, high-contrast and highresolution images at all magnifications. The electron source in combination with the excellent vacuum ensures a high brightness and a long life-time of both the tungsten and LaB6 filaments. To facilitate the practical work, one can store and re-load optical parameters like magnification and illumination settings, in conjunction with stage coordinates to re-allocate interesting areas of the specimen, at will. Moreover, alignments or operations parameters can be stored easily and retrieved at the push of a button. Each user has his or her own secure and customized microscope environment, and works with own or supervised settings and alignments. Microscope alignment efforts are reduced to a minimum so one can concentrate on getting the best out of the specimen. In addition, the user is given the flexibility to choose different imaging modes, e.g. rotation free magnification or isotropic chromatic aberration corrected magnification. In other words, the optical system can be adapted to the needs of biological research: easy zoom in or maximized sharpness, even for thicker samples. Figure 4 Left-hand shows a typical image at a low magnification on the Tecnai G 2 Spirit: it demonstrates the value of the patented high contrast performance. The right-hand image shows the high-resolution performance. For reasons of reference a graphite sample was used. 5
Page 1: Tecnai G 2 Spirit Highlights • Hi
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Tecnai™ G2 Spirit Product Brochure - FEI Company

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