Spider Silk Expression in Alfalfa

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Original silk constructs ‣Synthetic constructs based on N. clavipes sequence. ‣E. coli codon bias ‣MaSp 1 -> 16 repeats of 99 bp, each encoding: GGAGQGGYGGLGSQGAGRGGLGGQGAGAAAAAA GGX = 3 10 helix = elasticity (GA)n/(A)n = beta sheet = strength
Transformation of alfalfa Selective + hormones: formation of calli ‣ A. tumefaciens: LBA4404 ‣ Alfalfa: Regen S27 ‣Transformed ~120 leaves/clone ‣Explants moved to soil: 30/clone •kan •ticar •kinetin •2,4-D Selective – hormones: encourages root and shoot development
Page 1 and 2: Spider Silk Expression in Alfalfa M
Page 3 and 4: Why Spider Silk? Comparisons of Mec
Page 5 and 6: Why Plant Expression? ‣Low cost (
Page 7 and 8: Alfalfa (Medicago sativa L.) ‣ Pe
Page 9: Project Objectives 1) Insert a synt
Page 13 and 14: F1 Southern Blot analysis ‣Digest
Page 15 and 16: Western Blot Analyses MW G1 G3 B3 O
Page 17 and 18: WHY such low numbers? ‣Gene Silen
Page 19 and 20: New binary vector ‣ pCB301-kan-Ca
Page 21 and 22: Acknowledgements Randy Lewis, Ph.D.

Spider Silk Expression in Alfalfa

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