Spider Silk Expression in Alfalfa

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Parent Generation ‣MaSp1 + C #7 X WisFal ‣Analysis of F1 progeny – Southern (DNA level) – Western (Protein level)
F1 Southern Blot analysis ‣Digested alfalfa genomic DNA with the restriction enzyme NdeI. ‣Hybridized with a radioactively-labeled homologous probe. ‣ Examined images for the presence of a 2.3kb-hybridizing fragment, which indicated the silk gene was incorporated into the plant genome.
Page 1 and 2: Spider Silk Expression in Alfalfa M
Page 3 and 4: Why Spider Silk? Comparisons of Mec
Page 5 and 6: Why Plant Expression? ‣Low cost (
Page 7 and 8: Alfalfa (Medicago sativa L.) ‣ Pe
Page 9 and 10: Project Objectives 1) Insert a synt
Page 11: Transformation of alfalfa Selective
Page 15 and 16: Western Blot Analyses MW G1 G3 B3 O
Page 17 and 18: WHY such low numbers? ‣Gene Silen
Page 19 and 20: New binary vector ‣ pCB301-kan-Ca
Page 21 and 22: Acknowledgements Randy Lewis, Ph.D.

Spider Silk Expression in Alfalfa

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