Spider Silk Expression in Alfalfa

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Silk in spiders
Why Spider Silk? Comparisons of Mechanical Properties of Spider Silk Material Strength (Nm -2) Elasticity (%) Energy to Break (Jkg -1) Dragline Silk 4 x 10^9 35 4 x 10^5 KEVLAR 4 x 10^9 5 3 x 10^4 Rubber 1 x 10^6 600 8 x 10^4 Tendon 1 x 10^9 5 5 x 10^3__________ • Data derived from Gosline, J.M.; Dennv, M.W.; Demont, M.E. Nature 1984, 309,551
Page 1: Spider Silk Expression in Alfalfa M
Page 5 and 6: Why Plant Expression? ‣Low cost (
Page 7 and 8: Alfalfa (Medicago sativa L.) ‣ Pe
Page 9 and 10: Project Objectives 1) Insert a synt
Page 11 and 12: Transformation of alfalfa Selective
Page 13 and 14: F1 Southern Blot analysis ‣Digest
Page 15 and 16: Western Blot Analyses MW G1 G3 B3 O
Page 17 and 18: WHY such low numbers? ‣Gene Silen
Page 19 and 20: New binary vector ‣ pCB301-kan-Ca
Page 21 and 22: Acknowledgements Randy Lewis, Ph.D.

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