TRANSMISSION OF COWPEA APHID BORNE MOSAIC VIRUS ...

CHAPTER FOUR
RESULT
In the first experiment (Seed coat and cotyledon assay), CABMV was detected in the cotyledons in
'all the ten varieties of seeds analyzed serologically with ACP-ELISA. In contrast, the virus could not
,be detected in the seed coat of all the ten varieties of seeds analyzed.
While in the second experiment
(Tissue culture), all the leaves that were harvested from the cotyledons of the cowpea varieties that
.'were cultured in a Murashige and Skoog basal medium all tested positive to CABMV when analyzed
logically. Similarly, in the third experiment (Grown out test), all the leaves that were harvested
from the seeds germinated on the sterilized moistened filter paper in a sterilized Petri-dish all tested
,positive to CABMV when they were analyzed serologically with ACP-ELISA. This helped in
confirming that the virus was associated with the cotyledon of the cowpea seed.