TRANSMISSION OF COWPEA APHID BORNE MOSAIC VIRUS ...

3.0 MATERIALS AND METHOD 7
3.1 Experimental site 7
3.2 Materials used 7
3.3 Mechanical inoculation 7
3.3.1 Procedure for mechanical inoculation 8
3.4 Procedure for the separation of seed coat from the cotyledon 8
3.4.1 Seed coat and cotyledon test 9
3.5 Serological assay procedure using ACP-ELISA 9
3.5.1 Virus detection 9
3.5.2 Virus Indexing Protocol 10
CHAPTER FOUR
4.0 RESULTS
CHAPTER FIVE
5.0 DISCUSSION
5.1 CONCLUSION
5.2 RECOMMEDATION
REFERENCES