Bio imaging systems - Peqlab
Bio imaging systems - Peqlab
Bio imaging systems - Peqlab
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<strong>Bio</strong> Imaging Systems<br />
A top-quality bio <strong>imaging</strong> <strong>systems</strong> outstands<br />
with excellent sensitivity, lowest<br />
background noise and a sophisticated,<br />
yet intuitive software that along with<br />
just controlling the system also allows<br />
for image analysis and post-processing.<br />
PEQLAB's bio <strong>imaging</strong> <strong>systems</strong> meet<br />
with their technical innovations even the<br />
highest demands and set a new standard<br />
in the fields of fluorescence, chemiluminescence<br />
and bioluminescence <strong>imaging</strong>.<br />
HIGHEST SENSITIVITY<br />
Sensitivity is the key feature of a great<br />
<strong>imaging</strong> system, but depends on the<br />
harmonised interplay of multiple components<br />
like sensor, objective and darkroom.<br />
Especially the detection of faint<br />
fluorescence and chemiluminescence<br />
signals poses high challenges to sensitivity<br />
and <strong>imaging</strong> properties of an optical<br />
system. Only high tech monochromatic<br />
CCD sensors meet these demands, as<br />
they not only exhibit highest sensitivity,<br />
but also lowest background noise. All of<br />
PEQLAB's <strong>imaging</strong> <strong>systems</strong> are equipped<br />
only with specially manufactured 'scientific<br />
grade' CCD sensors and possess<br />
extremely light intense objectives. This<br />
highly coordinated top-quality hardware<br />
guarantees a maximum of image definition<br />
and fidelity. Additionally, with the<br />
usage of special lens <strong>systems</strong>, unwanted<br />
side effects like vignetting and image<br />
contortions are completely eliminated.<br />
LOWEST BACKGROUND<br />
Highest sensitivity can only be realised in<br />
combination with very low background<br />
noise, as otherwise faint signal would<br />
be overshadowed. Background noise<br />
is caused on the one hand thermically<br />
by infrared (IR) signals and on the other<br />
hand during the sensor readout by faintest<br />
leakage currents. PEQLAB‘s <strong>imaging</strong><br />
sys tems excel with lowest background<br />
noise due to their highly efficient double<br />
cooling system that constantly cools CCD<br />
sensor and camera chamber to reduce<br />
IR-noise. Continuing improvements in the<br />
field of cameras optics and optimisation<br />
of sensor readout complete the reduction<br />
of background noise.<br />
Moreover, PEQLAB‘s high quality darkrooms<br />
contribute to absolute background<br />
signal diminuition by totally eliminating<br />
interfering light and inner surface reflections.<br />
The darkrooms are fitted with<br />
special gaskets that guarantee pitch<br />
dark sealing, ban any light incidence<br />
and are internally lacquered with a<br />
special gap-free black anti-reflection<br />
coating. Another advantage of our <strong>systems</strong><br />
is the adjustable filter wheel and an extendable<br />
UV-table for preparative use.<br />
UV tables by PEQLAB impress with a<br />
very low heat build-up and a highly<br />
homogenous illumination. For most sensitive<br />
applications that demand a maximum<br />
contrast and minimal background<br />
'glow', the exclusive SUPER-BRIGHT<br />
UV tables are available. The innovative<br />
SUPER-BRIGHT technology minimises<br />
background 'glow' and displays exceptional<br />
homogeneity at the same time.<br />
This enables detection and quantification<br />
even of the faintest signals.<br />
INTUITIVE SOFTWARE<br />
Our purpose is to generate high quality<br />
image date that is a 1:1 reproduction of<br />
reality. Substantial scientific statements<br />
should only be based on unmodified<br />
raw-data. Possible modifications of<br />
any image raw data should always be<br />
under the user's control. Without exception<br />
our <strong>imaging</strong> <strong>systems</strong> and software<br />
were designed to fulfil the premise of<br />
highest data fidelity and integrity. The<br />
included licence-free software enables<br />
camera control, network compliance,<br />
image analysis and post-processing.<br />
Well arranged and intuitively to use,<br />
our software offers all functions that are<br />
needed to objectively and definitely<br />
evaluate the image data integrity and<br />
quality. Also for more sophisticated analyses<br />
like e.g. automatic lane detection,<br />
molecular weight and signal intensity<br />
calculations substantial software modules<br />
are already pre-installed.<br />
For even more complex analyses we<br />
also offer the <strong>Bio</strong>-1D software suite with<br />
many additional functions like e.g. densiometric<br />
quantifications and calculation<br />
of the apparent molecular weight. Of<br />
course we also offer network-licences<br />
(5-50) of the <strong>Bio</strong>-1D software for MS<br />
Windows Server.<br />
For the majority of our <strong>systems</strong> we do<br />
offer demonstrations in your laboratory<br />
- of course free of charge. Just ask!<br />
ORDERING INFORMATION • BIO IMAGING SYSTEMS<br />
Fon +49 9131 610 70 - 20 · Fax +49 9131 610 70 - 99 Freecall(D) 0800 100 2016 · Freefax(D) 0800 100 9016
FUSION FX7 Advance Chemiluminescence System<br />
Top class chemilumiscence/<br />
fluorescence combisystem:<br />
The allrounder with the technical<br />
specifications of a specialist<br />
Includes the sophisticated, yet<br />
intuitive analising and control<br />
software 'FUSION-CAPT'<br />
The Fusion FX7 Advance is a universal<br />
<strong>imaging</strong> system for chemiluminescence,<br />
bio luminescence, fluorescence and chemi<br />
fluorescence assays.<br />
With the Epi-UV module in the top of<br />
the darkroom it offers along with transillumina<br />
tion also the possibility of incident<br />
light fluorescence excitation. The Fusion<br />
FX7 Advance represents a sophisticated<br />
all-in-one solution for every common<br />
fluo res cence and chemiluminescence<br />
application.<br />
SPECIAL FEATURES<br />
- Highest sensitivity, great dynamic<br />
range and optimal resolution<br />
- Up-to-date supercooled CCD sensor<br />
(∆T= – 67°C)<br />
- Highly sensitive 4.2/10 megapixel<br />
CCD camera with 16 bit technology<br />
(65,536 grayscales)<br />
- PC-controlled, motorised fixed angle<br />
objective with autofocus<br />
- Innovative image acquisition modes<br />
for excellent results on the first run<br />
(even with unknown signal intensities)<br />
- Suitable for all commonly used<br />
chemiluminescence substrates<br />
- Powerful FUSION-CAPT control<br />
software suite for image analysis<br />
and post-processing<br />
HIGHEST IMAGE FIDELITY<br />
A hallmark feature of an outstanding<br />
chemiluminescence <strong>systems</strong> is the high<br />
quality of the optics and the CCD sensor.<br />
The Fusion FX7 Advance excells<br />
in image quality with its impressive<br />
4.2/10 megapixel 16 bit (65,536<br />
grayscales) CCD sensor. Due to the<br />
extremely high number of pixels the sensitivity<br />
can be increased up to 64 times<br />
by merging adjacent pixels ('binning').<br />
Thereby the Fusion FX7 Advance is<br />
the ideal system for every kind of chemiluminescence,<br />
bioluminescence or in<br />
vivo luminescence analysis and meets<br />
the highest demands for resolution and<br />
image quality.<br />
HIGHEST SENSITIVITY<br />
The Fusion FX7 Advance offers a<br />
fixed angle objective with extremely<br />
high lens speed (f: 0.84) and autofocus<br />
that guarantees crispiest images even<br />
at short distances (< 20 cm) without the<br />
need of an additional close-up lens.<br />
The short distance and the omission of<br />
the close-up lens avoid loss of signal<br />
intensity and allow for compact design.<br />
SUITABLE FOR THE FOLLOWING<br />
METHODS AND SUBSTRATES<br />
Chemiluminescence<br />
AceGlow, SuperSignal,<br />
ECL, ECL Plus, ECL–Advance,<br />
CSPD, CDP Star<br />
Fluorescence DNA/RNA <strong>imaging</strong><br />
Ethidiumbromide, Gel Star,<br />
SYBR Green, SYBR Gold<br />
Fluorescence and classical<br />
protein <strong>imaging</strong><br />
SYBRO Orange/Ruby/Red,<br />
Texas Red Fluorescein,<br />
Qdot, GFP, RFP, YFP,<br />
Coomassie Brilliant Blue, Silver Star<br />
Examples of experimental<br />
applications<br />
Analysis of Western blot membranes<br />
and thin layer chromatography sheets<br />
(fluorescence, 'shadowing'), in vivo<br />
fluorescence and luminescence (also<br />
in MTP-format)<br />
ORDERING INFORMATION • FUSION FX7 ADVANCE CHEMILUMINESCENCE SYSTEM<br />
PRODUCT DESCRIPTION CAT. NO.<br />
FUSION FX7 Advance Chemiluminescence/fluorescence <strong>imaging</strong> system 60-FU-26MA<br />
Chemiluminescence/fluorescence <strong>imaging</strong> system<br />
with Epi-UV and SUPER-BRIGHT technology<br />
60-FUUV-26MXA<br />
Fon +49 9131 610 70 - 20 · Fax +49 9131 610 70 - 99 Freecall(D) 0800 100 2016 · Freefax(D) 0800 100 9016
Chemiluminescence Systems Overview<br />
MODEL<br />
Application<br />
Fusion FX7<br />
Advance<br />
Fusion SL<br />
Advance<br />
Fusion Solo<br />
Chemiluminescence yes yes yes yes<br />
<strong>Bio</strong>luminescence yes yes yes yes<br />
Fluorescence yes optional no optional<br />
Chemifluorescence yes optional no optional<br />
Imaging properties<br />
Fusion Xpress<br />
'Scientific grade' CCD camera yes (USB) yes (USB) yes (USB) yes (FireWire)<br />
16 bit grayscale yes yes yes yes<br />
Sensor resolution 4.2 megapixel 4.2 megapixel 4.2 megapixel 2.0 megapixel<br />
Maximum resolution (interpolated) 10 megapixel 10 megapixel 10 megapixel 5.5 megapixel<br />
Dynamic range 4.8 decades 4.8 decades 4.8 decades 4.8 decades<br />
Camera cooling ∆T = – 67 °C ∆T = – 67 °C ∆T = – 67 °C ∆T = – 50 °C<br />
Image transmission rate (10 images/s) yes yes yes yes<br />
Background < 2 % < 2 % < 2 % < 4 %<br />
Objective<br />
Fixed angle (f:0.84)<br />
motorised<br />
Fixed angle (f:0.84)<br />
manual<br />
Fixed angle (f:0.84)<br />
manual<br />
Autofocus yes no no yes<br />
Zoom (f:1.2)<br />
motorised<br />
ORDERING INFORMATION • FUSION CHEMILUMINESCENCE SYSTEMS OVERVIEW<br />
Maximum viewing range 23 cm x 23 cm 23 cm x 23 cm 16 cm x 16 cm 20 cm x 26 cm<br />
Darkroom<br />
UV/blue transilluminator yes optional no optional<br />
Epi-UV/blue optional optional no optional<br />
SPECTRA-module (RGB/NIR) optional optional no optional<br />
Motorised filterwheel yes yes no yes<br />
Automatic tray position recognition yes no no yes<br />
Number of tray positions 5 5 4 2<br />
Automatic illumination yes no no yes<br />
Software<br />
PC-controlling yes yes yes yes<br />
Pre-configured image modes yes yes yes yes<br />
Quantification yes yes yes yes<br />
Absolute image data integrity yes yes yes yes<br />
'Binning' (2 x 2 to 8 x 8) optional optional optional optional<br />
Interpolation optional optional optional optional<br />
Data export to Excel yes yes yes yes<br />
Weights and measurements<br />
Weight 58 kg 48 kg 25 kg 48 kg<br />
Measuments in cm (H x W x D) 95 x 42 x 44 95 x 42 x 44 43 x 35 x 35 95 x 42 x 44<br />
Fon +49 9131 610 70 - 20 · Fax +49 9131 610 70 - 99 Freecall(D) 0800 100 2016 · Freefax(D) 0800 100 9016