DAKO Autostainer Universal Staining System

CIHRT Exhibit P-2339 Page 1 

For further information pleaseffice' 

contact your nearest DAKO 0 . 

Australia 

Tel. 2 9316 4633 

Fax 2 93164773 

Austria 

Tel. 0800 0800 7153 

Fax 0800 0800 7154 

Belgium 

Tel. 0163872 20 

Fax 016 38 72 21 

DAKO Autostainer 

Universal Staining 

System 

Canada 

Tel. 905 858 8510 

Fax 905 858 8801 

Czech Republic 

Tel. 05-41423710 

Fax 05-41 423711 

Denmark 

Tel. 44 85 95 00 

Fax 44 85 95 95 

France 

Tel. 1 30 50 00 50 

Fax 1 30 50 00 11 

Germany 

Tel. 04069 69 470 

Fax 040 69 52 741 

Italy 

Tel. 02 58 078 231 

Fax 02 58 078 292 

Japan 

Tel. 075 211 3655 

Fax 075 211 1755 

The Netherlands 

Tel. 0204211100 

Fax 020 42 11 101 

Norway 

Tel. 23 14 05 40 

Fax 23 14 05 42 

Poland 

Tel. 058-661 1879 

Fax 058-661 3390 

Spain 

Tel. 93 499 05 06 

Fax 93 499 02 08 

Sweden 

Tel. 08 556 20 600 

Fax 08 556 20 619 

Switzerland 

Tel. 041 76011 66 

Fax 041 76011 77 

United Kingdom 

Tel. (0)1 353 66 99 11 

Fax (0)1 353 66 89 89 

United States of 

America 

Tel. 805 566 6655 

Fax 805 566 6688 

International Web site 

www.dako.com 

Distributors in . 

more than 40 countnes 

OAKoautostainer 

• Instrument 

• Optimized reagents 

• User-friendly software 

• Technical support 

• Research and development 

• Quality assurance 

• Superior staining quality


~I 

AKO 

DAKO - The Total Solution 

in Automated Immunohistochemistry 

More than Just an Instrument 

Mammary carcinoma 

stained with Anti 

Progesterone Receptor, 

code No. N 1630, using the 

DAKO LSAS"2 System. 

The modern pathology laboratory is faced 

with increasing demands on productivity, 

cost-efficiency as well as quality and 

consistency of results. 

The answer to this challenge is automation 

- provided as a total solution. 


stained with Anti-Estrogen 

Receptor a, code No. 

N 1575, using the 

DAKO LSAso2 System. 

Up to 48 

slides can 

be individually 

programmed and 

processed in a 

given run. 

DAKO has a 30-year track record as the leader 

in immunohistochemistry. The combination of 

reagent and instrument supply enables DAKO 

to offer solutions for today's laboratory 

demands. 

The DAKO Autostainer is a proven and 

validated system within the DAKO 

organization, backed by optimized reagents, 

skilled support and qualified service engineers. 

When you choose DAKO as an instrument 

and reagent supplier, you acquire more than 

just performance. 

• Technical partnership 

DAKO provides reference material, 

dedicated technical application 

support and user training. 

• Instrument support 

DAKO offers multiple options of service 

agreements to fit your specific needs. 

You will always be served by a local 

service engineer who understands 

your situation. 

• Reagent reliability 

Our long experience in the field of 

immunohistochemistry offers you the 

best guarantee of reliable and validated 

products. DAKO develops products based 

on input from you and your colleagues 

around the world. 

• Financial partnership 

It is a well-known fact that automation 

brings cost-benefits. At DAKO we are 

prepared to offer you customized 

solutions that consider your workload, 

inventory supply, and need for service 

and support. 

Access up to 

64 different reagents 

by using both 

racks; program 

complex protocols 

and/or mUltiple 

protocols 

simultaneously. 

Hodgkin's lymphoma 

stained with Anti-CD30, 


DAKO EnVision System. 


stained with Anti-Cytokeratin, 


DAKO EnVision System. 

Tonsil stained with Anti-SCL2 

Oncoprotein, code No. 

M 0887, using the DAKO 

CSA System. 

Tonsil stained with Anti 

CD23, code No. M 0763, 

using the DAKO 

CSA System 

Teflono-coated precisJon tip 

provides dependabl results without 

the need for freque t replacements 

M OAKO 

It 

or 

is 

the 

specially 

nuisance 

design 

of d sposable:'~~_~==::::::::::,:~~~~~"'''IIIIIIIIII'!II!!II!==:~~:~ 

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AKO 

DAKO Autostainer Reagents 

DAKO is acknowledged as the world leader 

in the development of antibodies and 

detection systems. DAKO has the largest 

panel of diagnostic antibodies and is the 

leading company within lymphoma and 

leukaemia immunophenotyping. 

ready-to-use antibodies, detection systems 

and accessories. 

All dedicated reagents are available in 

pre-filled Autostainer vials for convenience, 

ease of operation and time savings. 

Immunostaining Systems 

Product 

Code No. 

Universal LSAB2 Kit/HRP, Rabbit/Mouse K 0675 

Universal LSAB+ KitlHRP. Rabbit/Mouse/Goat K0690 

Link (10 x 11 mL) 

Streptavidin Peroxidase (10 x 11 mL) 

Accessory Products 

Product 

Hematoxylin (500 mL) 

Peroxidase-Blocking Reagent (10 x 11 mL) 

Protein Block, Serum Free (10 x 11 mL) 

Proteinase K, Prediluted (10 x 11 mL) 

Universal Negative Control, Ready-to-Use. 

Code No. 

S 3301 

S 2001 

X 0909 

S 3020 

N 1698 

We have developed a comprehensive range of 

dedicated reagents for the DAKO Autostainer. 

The reagents include a wide range of 

We provide empty vials and a range of 

optimized protocols for ready-to-use 

reagents that are not yet available in 

pre-filled vials. 

Substrate Chromogens 

Product 

Code No. 

DAKO AEC Substrate-Chromogen, Ready-To-Use K 3464 

DAKO AEC+ SUbstrate-Chromogen, Ready-Te-Use K 3469 

DAKO Liquid DAB K 3466 

for N-Series Mouse Primary Antibodies 

Universal Negative Control, Ready-to-Use, 

for N-Series Rabbit Primary Antibodies 

Wash Buffer, Concentrated x 10 (1 L) 

N 1699 

S3006 

DAKO Liquid DAB+ K 3468 

(10 x 11 mL) 

Products Packaged for the DAKO Autostainer 

Ready-to-use Antibodies· 

Product 

Rb a Hu Alpha-1-Fetoprotein 

Mo a Hu BCL2 Oncoprotein, Clone 124 

Mo a Hu Carcinoembryonic Antigen, Clone 11-7 

Rba Hu Carcinoembryonic Antigen 

Rba Hu CD3, TCeli 

Moa Hu CD20cy, B Cell, Clone L26 

Moa Hu CD30, Ki-1 Antigen, Clone Ber-H2 

Moa Hu CD45RO, T Cell, Clone UCHL-1 

Rb a Hu Chromogranin A 

Mo a Hu Cytokeratin, Clone AE1/AE3 

Mo a Hu Cytokeratin. Clone MNF116 

Moa Hu Cytokeratin. High Molecular Weight, Clone 34~E12 

Moa Hu Cytokeratin 8, Low Molecular Weight, Clone 35~H11 

Moa Hu Desmin, Clone 033 

Moa Hu Epithelial Membrane Antigen, Clone E29 

Moa Hu Estrogen Receptor fl., Clone 105 

Rb aCw Glial Fibrillary Acidic Protein 

Rb a Hu Kappa Light Chains 

RbaCw Keratin, Wide Spectrum Screening 

Mo a Hu Ki-67 Antigen, Clone MIB-1 

Rba Hu Ki-67 Antigen 

Rb a Hu Lambda Light Chains 

Moa Hu Leucocyte Common Antigen, Clones 2B11 + PD7/26 

Mo a Hu Melanosome, Clone HMB-45 

Mo a Hu Muscle Actin, Clone HHF35 

Mo a Hu Neuron-Specific Enolase. Clone BBS/NCNI-H14 

Rba Neuron-Specific Enolase 

Moa Hu p53 Protein, Clone 00-7 

Mo a Hu Progesterone Receptor, Clone 1A6 

Rb a Hu Prostate-Specific Antigen 

Code No. 

N 1501 

N 1587 

N 1586 

N 1503 

N 1580 

N 1502 

N 1558 

N 1520 

N 1535 

N 1590 

N 1523 

N 1553 

N 1560 

N 1526 

N 1504 

N 1575 

N 1506 

N 1510 

N 1512 

N 1633 

N 1574 

N 1513 

N 1514 

N 1545 

N 1567 

N 1557 

N 1516 

N 1581 

N 1595 

N 1517 

Products packaged 

for use on the 

DAKO Autostainer 

offer great 

convenience. 

Detection Systems of 

Several Performance Levels 

DAKO has a range of detection systems 

available for the DAKO Autostainer, covering 

a wide spectrum of routine and research 

applications. 

• LSAB2 is a routine detection system. 

For optimal convenience and fastest 

turn around time, ready-to-use primary 

antibodies are available. 

• EnVision detection systems are 

available as routine as well as high 

sensitivity (+) systems. 

• CSA" (Catalyzed Signal Amplification) 

is a powerful and unique detection 

system for "difficult" mouse and rabbit 

antibodies. It is up to 200 times more 

sensitive than conventional biotin methods. 

Slide-specific protocols including reagent 

volume and dispense patterns, thus 

optimizing reagent usage (here shown with 

multi-tissue sections 

on the 

slides). 

Rb a Hu 

RbaCw 

RbaCw 

Moa Hu 

Rba Hu 

Moa Hu 

Rb a Hu 

Prostatic Acid Phosphatase 

S100 

S100 A 

Smooth Muscle Actin. Clone 1A4 

Synaptophysin 

Vimentin, Clone V9 

Von Willebrand Factor 

N 1518 

N 1573 

N 1519 

N 1584 

N 1566 

N 1521 

N 1505 

• LSAB+ is a high sensitivity detection system. 

• EnVision* is based on a patented, 

labelled polymer technology. This biotin-free, 

two-step detection system eliminates 

• DAKO ARKTM (Animal Research Kit) is a 

sensitive system ideal for mouse monoclonal 

antibodies on mouse tissue without 

causing background staining. 

'Diluted antibody (2 x 11 mL) and negative control (11 mL) 

non-specific staining from endogenous 

biotin and provides more specific staining. 

* U.S. Patent 5,543.332 and European Patent 594,772. 

** The CSA System embodies technology developed 

by and licensed from NEN (U.S. Patent 5,196,306).

,~ ,~ 


~AKO 

Intelligent Automation and Software 

Flexible and fast programming of each run. 

The versatile DAKO Autostainer simplifies 

your workload and offers consistent staining 

results. 

The DAKO Autostainer software is flexible 

yet simple and intuitive, making it easy to set 

up a staining run. The laboratory technologist 

will soon be confident in operating the 

instrument, increasing the flexibility and 

reliability of your laboratory. 

customize a protocol for each individual 

slide. The option to adjust reagent volume 

and reagent dispense zone minimizes the 

reagent consumption without compromising 

staining quality. 

The system mimics manual staining, while 

improving lab-to-Iab and operator-to-operator 

consistency. To protect personnel and 

environment the system segregates hazardous 

from non-hazardous waste. There is a very 

Technical Specifications 

Dimensions 

Weight 

Electrical specifications 

Normal operating temperature 

Slide rack capacity 

Total slide capacity 

Reagent capacity 

Reagent dispense volumes 

Reagent probe volume capacity 

Fluid handling components 

Width 102 cm, Depth 69 cm, Height 61 cm 

63.5 kg 

220 V: 220/240 V (± 10%), 50 Hz (±2 Hz) 

(120 V: 110/120 V (± 10%), 60 Hz (±2 Hz)) 

Ambient 18-26 "C 

1-12 slides 

1-48 slides 

64 different reagents (15 mUreagent vial) 

100, 150, 200, 400 and 600 ~L 

1.2 mL 

,)', High-precision, fixed stainless steel, Teflon~-coated reagent probe 

(designed to minimize protein adsorption), independent wash and blow elements 

f' 

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Program Slides ""--" 

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l2:l'-T'Ul.'idcZ211.3)wilh400pl 

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Run log displaying current step and recording 

total run activity. 

The DAKO Autostainer Universal Staining 

System is an automated horizontal slide 

processing system optimized with DAKO 

reagents for the staining of formalin-fixed, 

paraffin-embedded tissues, frozen sections, 

cytospins, cell smears and fine needle 

aspirates. It consists of a slide processing 

unit, a desktop personal computer, software 

and a printer. The DAKO Autostainer is an 

open reagent system with an easy-to-use 

Windows 95/98 software that allows for 

pre-programmed and customized protocols. 

Instrument 

Product 

DAKO Autostainer, Universal Staining System 

Accessory Products 

Product 

Autostainer, Drain Tube Kit 

Autostainer, Reagent Racks (2 Racks) 

Autostainer, Reagent Vials (100 Vials) 

Autostainer, Slide Clips (12 Clips) 

Autostainer, Slide Rack 

Autostainer Demonstration Pack 

DAB-Away, Cleaning Agent 

Clear-It Cleaning Reagent 

DAKO Seymour Glass Slide 

Labelling System 

Product 

DAKO Seymour Glass Slide Labelling System 

DAKO Seymour Label Kit 

Code No. 

S3400 

Code No. 

S 3423 

S 3424 

S 3425 

S 3422 

S 3396 

S 3410 

S 1967 

SL 002 

Code No. 

S 3416 

S 3417 

The DAKO Autostainer 

Demonstration Pack 

includes a selection of 

two antibodies with 

matched positive 

control tissues, 

visualization 

system of choice and 

accessory reagents to 

demonstrate the 

performance and 

quality of results. 

The versatile DAKO Autostainer simplifies your workload as it enhances consistency 

• Walk-away convenience 

• Automated documentation 

• Mimics manual staining 

and avoids retraining 

• Easy set up and flexible programming 

• Consistent results and fewer repeats 

decrease costs 

• Enables inter and intralaboratory consistency 

• Allows simultaneous staining of multiple 

markers 

• Autoprogramming for convenient selection 

of protocols 

• DAKO Seymour Glass Slide Labelling 

System provides sample identification 

throughout slide processing

DAKO Autostainer Universal Staining System

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