Agronomic Investigation of New Microbial Isolates as Bio fertilizers ...
Agronomic Investigation of New Microbial Isolates as Bio fertilizers ...
Agronomic Investigation of New Microbial Isolates as Bio fertilizers ...
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Methodology<br />
a. Microbiological work<br />
Survey and collection <strong>of</strong> soil samples - High biodiversity hot spot<br />
are<strong>as</strong> <strong>of</strong> South India<br />
<strong>Microbial</strong> ( bacteria, fungi & actinomycetes) enumeration - Serial<br />
dilution and plate counting<br />
From the bacterial population, screening for P solubilizers -<br />
Pikovskaya’s agar media<br />
N fixers - Jensen’s nitrogen free solid medium<br />
P solubilizing capacity- Vanado molybdo phosphoric yellow colour<br />
method<br />
N fixing capacity - Kjeldhal method<br />
b. Preparation <strong>of</strong> bi<strong>of</strong>ertilizer<br />
<br />
<br />
M<strong>as</strong>s multiplication <strong>of</strong> potent isolates<br />
100 ml <strong>of</strong> the broth containing the isolates mixed with sand and<br />
charred rice husk (1:4) <strong>as</strong>eptically
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