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Other Activities - Central Drug Research Institute

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Major Events Organized<br />

A practical training course on “Isolation and<br />

separation of proteins and their detection by<br />

immunoblotting”<br />

A practical training course on<br />

“Isolation and separation of proteins and<br />

their detection by immunoblotting” was<br />

held from 28th-29th Jan 2013 at<br />

Proteomics and Cancer biology Lab,<br />

DTDD Division. CSIR-CDRI conducted by<br />

Dr. Arun Kumar Trivedi in collaboration<br />

with Merck Millipore. Workshop included<br />

hands on training on isolation of proteins<br />

from mammalian cells, separation of<br />

isolated proteins on 1D SDS-PAGE and<br />

their detection using specific antibody after immunoblotting by<br />

conventional as well as SNAP-i.D. methods. In addition, visualization<br />

of protein bands by coomassie blue staining and colloidal coomassie<br />

staining was also demonstrated. This workshop also included a<br />

lecture by Dr. Trivedi on kinds of lysis buffer, ingredients of buffer<br />

and their role, principal of immunoblotting and troubleshooting. This<br />

workshop was attended by PhD aspirants from various intuitions<br />

viz. Dr. Ram Manohar Lohia <strong>Institute</strong> of Medical Sciences (RMLIMS),<br />

King George`s Medical University (KGMU), Amity University and<br />

different divisions of CSIR-CDRI of Lucknow.<br />

38 th Sir Edward Mellanby Memorial Oration<br />

In memory of Sir<br />

Edward Mellanby, Founder<br />

Director, CSIR-CDRI, the 38 th<br />

Mellanby Memorial Lecture was<br />

organized on 11 February 2013.<br />

The lecture was delivered by<br />

Dr.V. Craig Jordan, Professor<br />

of Oncology and Pharmacology,<br />

Lombardi Comprehensive<br />

Cancer Center, Georgetown<br />

University, USA. The topic of<br />

his presentation was Four<br />

decades of discovery for the<br />

treatment and prevention of<br />

breast cancer: The SERM story.<br />

The SERM story has produced<br />

a remarkable insight not only for<br />

the treatment of multiple diseases in women, but also provided<br />

explanations for the mystery of oestrogen-induced apoptosis.<br />

Annual Report 2012-13 83

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