Progress report summarizing the reef fish sampling, PCB - Earthjustice

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Fish not retained for sampling (undersize, non-target, predator damaged fish) were identified, measured, documented in a logbook and released overboard. Non target fish released included transient reef fish like amberjacks, species like great barracuda not typically consumed by the local fishing population, and small reef fish like bank sea bass and tomtates not normally retained by anglers for human consumption. In addition all sublegal target fish and all predator damaged fish were discarded (Table 9). I (D) (8) Laboratory Sample Receipt and Sample Analysis The receiving analytical laboratory used for all of the Oriskany Reef fish PCB analyses was the Geochemical and Environmental Research Group (GERG) of the College of Geosciences and Maritime Studies at Texas A & M University, 833 Graham Road, College Station, Texas 77845 (http://gerg.tamu.edu ). Dr. Terry Wade, Deputy Director, Environmental Science is the interim Lab Director (ph. 979.862.2323 x 134; email: terry@gerg.tamu.edu ). Dr. Wade took GERG former Lab Director, Dr. Guy Denoux’s place early in the study as our point of contact after Dr. Denoux passed away. Dr. Denoux had been our initial very helpful GERG contact and was so noted in the Monitoring Work Plan (Dodrill and Turpin, 2007). Laboratory sample handling, analysis and reporting procedures followed the Laboratory Quality Assurance Project Plan prepared by GERG for FWC and ECMRD and identified as GERG Manual 0401 in Appendix K of the Monitoring Work Plan. In accordance with GERG SOP 9706 (Procedure for Receiving Samples), upon receipt of the samples, GERG staff filled out a sample receiving/integrity report, noting sender, shipping company, time of sample receipt, number of containers received, condition of custody seals, presence of chain-of-custody records, general sample condition, number of samples received, name of the individual who checked the shipment in, any problems or discrepancies, and if so what the resolution was. During the eight rounds of collected reef fish shipped to GERG, three discrepancies were noted. Two discrepancies involved incorrect transcription by of a Sample ID number onto the list of shipped specimens received by GERG. These discrepancies were corrected. A third discrepancy revealed that one of the packaged red snapper samples (OR-RS-057) had been inadvertently discarded at night during the process of removing wet ice and repacking and storing the samples in the EPA lab cooler prior to shipping to GERG. As a result only 29 instead of 30 specimens were 36 | P age
actually shipped to GERG on February 17, 2007. No further repacking of fish specimens at night took place during the study. Tracking procedures were conducted in accordance with GERG SOP 9708. A laboratory sample identification number was assigned to each sample to correspond with the project’s Sample Identification Number. The ECMRD project Co-Manager was notified by GERG of sample receipt and condition and he in turn notified the FWC Project Co-Manager. Samples were then stored at GERG in accordance with GERG SOP 9707 (Procedures for storing samples) at -20º (plus or minus 10º) Celsius until analytical procedures began. When ready for analysis in the labratory, each fish was scaled, washed with deionized water and a minimum of 100 gram skin-on lateral muscle skin-on sample extracted from each fish was homogenized. Three sample aliquots of the homogenate were weighed out and available for the sample of which about 10 grams wet weight of homogenized tissue was used for a sample analysis. The other two aliquots from each individual fish sample were used for Matrix Spike analysis or running a duplicate analysis sample if required. A duplicate sample is not from a separate homogenate or from different tissue areas of the fish. For a reanalysis, the original homogenate is utilized, though a new homogenate can be made from the other fillet if it is specifically requested. For sample duplicates, the first sample logged in for a sample batch is a duplicate and matrix spike, and then the first sample logged in of the second sample batch is similarly treated. The remaining sample homogenate amount for the other samples is available for a Matrix Spike analysis or duplicate sample analysis if requested. Samples were extracted using standard tissue extraction procedures. Surrogates for these analyses were added prior to extraction. Samples were processed by standard GERG methods and analyzed for 209 PCB congeners by high resolution mass spectrometry. The tissue samples were extracted, purified and analyzed using the following protocols contained in the following Standard Operating Procedures (SOPs) used at GERG: SOP-9807- Extraction of Biological Tissues for the Analyses of Polynuclear Aromatic Hydrocarbons, Polychlorinated Biphenyls, and Organochlorine Pesticides. (Revision 1 as of October 27, 1998, See Appendix L of Monitoring Work Plan). EPA 1668A- Chlorinated biphenyls Congeners in Water, Soil, Sediment, Biosolids and Tissues by High Resolution Gas Chromatography/ High Resolution Mass Spectrometer 37 | P age
Page 1 and 2: Progress report summarizing the ree
Page 3 and 4: EXECUTIVE SUMMARY In compliance wit
Page 5 and 6: LIST OF FIGURES Figure Page No. 1.
Page 7 and 8: I (A) (1) EPA Oriskany Reef PCB Tie
Page 9 and 10: validate the Navy developed PCB fat
Page 11 and 12: that “the numbering standard for
Page 13 and 14: ship material leachate experiments
Page 15 and 16: solid PCB containing materials woul
Page 17 and 18: EPA review included: 1) a human hea
Page 19 and 20: I(C)(6) PCB Containing Materials On
Page 21 and 22: I(C) (8) Oriskany Reef Created In a
Page 23 and 24: fishes for the Monitoring Work Plan
Page 25 and 26: I (D) (2) Fishing Vessel Sampling P
Page 27 and 28: long from the top to the mouth of t
Page 29 and 30: During sample round #2 all four che
Page 31 and 32: The rod and reel manpower sampling
Page 33 and 34: was intentionally scheduled after t
Page 35: weren’t running after each fish w
Page 39 and 40: samples during Sampling Rounds 1-8
Page 41 and 42: harvesting at least 15 legal size r
Page 43 and 44: attraction as expressed by an incre
Page 45 and 46: Of these 10 reef assessment dives,
Page 47 and 48: assessments which were limited to t
Page 49 and 50: not considered target species for a
Page 51 and 52: have had any PAH levels approaching
Page 53 and 54: Oriskany, and one recapture at a
Page 55 and 56: ed snapper season commencement. The
Page 57 and 58: Table 8a. List of reef fish species
Page 59 and 60: Oriskany Reef during Sampling Round
Page 61 and 62: Species Composition of Oriskany Sam
Page 63 and 64: The GERG lab reported their receipt
Page 65 and 66: pectoral fins of the blackfin snapp
Page 67 and 68: Trophic level or position Trophic p
Page 69 and 70: that means after five years only 3%
Page 71 and 72: II (D) (1) (c) Red Snapper Discussi
Page 73 and 74: 1,000,000 ΣPCB (pg/g) a. Sample Da
Page 75 and 76: The PCB levels measured from red sn
Page 77 and 78: fins relatively short, not reaching
Page 79 and 80: Results of PCB analysis by sample d
Page 81 and 82: ody, these spots giving a yellow-st
Page 83 and 84: II (D) (3) (c) Red Porgy Discussion
Page 85 and 86: cheeks that are speckled with brigh
Page 87 and 88:
II (D) (4) (c) Whitebone Porgy Disc
Page 89 and 90:
(common) name. The first spine is l
Page 91 and 92:
II (D) (5) (c) Gray Triggerfish Dis
Page 93 and 94:
The Scamp inhabits continental shel
Page 95 and 96:
Results of PCB analysis by sample d
Page 97 and 98:
species was not among the first 30
Page 99 and 100:
elieved to be unlikely since most o
Page 101 and 102:
trip less than 15% of the time due
Page 103 and 104:
II F (6) (b) Current Florida Marine
Page 105 and 106:
assessment, technological feasibili
Page 107 and 108:
III Oriskany Reef Monitoring Projec
Page 109 and 110:
Fishbase.org (Internet site). 2011.
Page 111 and 112:
Ingram, G.W., Jr. and W.F. Patterso
Page 113 and 114:
Patterson, W. F., J. H. Cowan Jr, e
Page 115 and 116:
Thompson, M.J., W.W. Schroeder, and
Page 117 and 118:
Table 2. FWC Fish Census data taken
Page 119 and 120:
Table 4. Fish census data taken by
Page 121 and 122:
frogfish Antennarius sp.
Page 123 and 124:
eef butterflyfish Chaetodon sedenta
Page 125 and 126:
Vermilion snapper Rhomboplites auro
Page 127 and 128:
OR-RS-042 Red Snapper 4/12/2007 HnL
Page 129 and 130:
OR-RS-155 Red Snapper 11/18/2009 Hn
Page 131 and 132:
Table 14. Tissue sample characteris
Page 133 and 134:
Table 15. Percent composition of to
Page 135 and 136:
Table 16b. Percent composition of t
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Progress report summarizing the reef fish sampling, PCB - Earthjustice

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