Desmoglein 3 + Napsin A Cocktail

Desmoglein 3 + Napsin A Cocktail
Prediluted Multiplex Cocktail (4-Step)
Control Number: 901-428DS-021611
ISO
9001:2000
CERTIFIED
Catalog Number:
Description:
Dilution:
Diluent:
PPM 428DS AA
6.0 ml, prediluted
Ready-to-use
N/A
Intended Use:
For In Vitro Diagnostic Use
Summary and Explanation:
Desmoglein 3 (DSG3) is a calcium-binding transmembrane glycoprotein component of
desmosomes in vertebrate epithelial cells. Studies have shown DSG3 to have 83-95%
sensitivity and 100% specificity in detecting squamous cell carcinoma (SqCC) vs. lung
adenocarcinoma. DSG3 is associated with shorter survival for all lung cancer patients
regardless of the histologic subtype (5-year survival of 20.9% vs. 49.5%, P < .001).
Patients with atypical carcinoid tumors, lacking Desmoglein 3 expression showed a 5-
year survival of 0% compared with 36.8% for DSG3 positive cases (P < .001).
Napsin A is expressed in type II pneumocytes of normal lung and in adenocarcinomas
of the lung and kidney. Studies have shown that Napsin A is more sensitive (80-87%)
and more specific marker than TTF-1. Napsin A is 100% specific for lung
adenocarcinoma vs. 100% negative in lung SqCC. Napsin A used in combination with
TTF-1 provides 93% sensitivity and 100% specificity for lung adenocarcinoma, if CK5
and Desmoglein 3 are both negative in the same section.
DSG3 is a cell membrane stain that marks lung SqCC (DAB). Napsin A is a
cytoplasmic/granular stain that marks lung adenocarcinomas (Fast Red). In the vast
majority of lung cancers tested, only a single antibody stain will be observed. Coexpression
of both antibodies may be observed in adenosquamous cell carcinomas, or
in some cases residual normal lung will stain with Napsin A. Desmoglein 3 + Napsin
A are very sensitive and specific markers for discriminating between lung SqCC and
lung adenocarcinoma. This antibody cocktail is extremely accurate and is 100%
specific. In grades 1-2, Desmoglein 3 + Napsin A provide staining sensitivity in the
mid 90% range; thus the antibody cocktail of Desmoglein 3 + Napsin A is a first-line
screener for discriminating between lung adenocarcinoma vs. lung SqCC.
Principle of Multiplex Staining:
A Multiplex IHC stain can be accomplished in four major steps. The initial step
consists of an antibody cocktail with at least one mouse and one rabbit antibody. This
cocktail is applied to the tissue and will bind with two or more target antigens. A
multiplex detection cocktail of horseradish peroxidase (HRP) and alkaline phosphatase
(AP) conjugated secondary antibodies is applied. The third step consists of the addition
of DAB-Substrate that binds to the HRP and produces a brown chromogenic reaction
product. The fourth step consists of a Fast Red-Substrate that binds to the AP and
produces a red chromogenic reaction product.
Source: Mouse monoclonal and Rabbit polyclonal
Species Reactivity: Human, others not tested
Clone: BC11 + N/A
Isotype: IgG1 + N/A
Epitope/Antigen: Desmoglein 3 + Napsin A
Cellular Localization:
Desmoglein 3 (Membrane): Brown
Napsin A (Cytoplasmic - granular): Red
Positive Control: Lung squamous cell carcinoma and lung adenocarcinoma
Normal Tissue: Skin or tonsil (DSG3); lung (Napsin A)
Abnormal Tissue: Lung squamous cell carcinoma and lung adenocarcinoma
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Supplied As: Buffer with protein carrier and preservative.
Protocol Recommendations
Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment Solution (recommended): Diva
Pretreatment Protocol:
Heat Retrieval Method:
Retrieve sections using Biocare’s Decloaking Chamber at 125°C for 30 seconds. Allow
solution to cool for 10 minutes then wash in distilled water
Protein Block:
Incubate for 10 minutes at RT with Biocare's Background Punisher.
Primary Antibody:
Incubate for 30 minutes at RT.
Double Stain Detection:
Incubate for 30 minutes at RT using Biocare's MACH 2 Double Stain 2.
Chromogen (1): Incubate for 5 minutes at RT with Biocare's Betazoid DAB.
Chromogen (2):
Incubate for 5-7 minutes at RT with Biocare's Warp Red. Rinse in deionized water.
Counterstain:
Counterstain with Hematoxylin. Rinse with deionized water. Apply Tacha's Bluing
solution for 1 minute. Rinse with deionized water.
Technical Note:
This antibody has been standardized with Biocare's MACH 2 Double Stain 2. It can
also be used on an automated staining system. Use TBS buffer for washing steps.
Performance Characteristics:
The optimum antibody dilution and protocols for a specific application can vary. These
include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue
section thickness and detection kit used. Due to the superior sensitivity of these unique
reagents, the recommended incubation times and titers listed are not applicable to other
detection systems, as results may vary. The data sheet recommendations and protocols
are based on exclusive use of Biocare products. Ultimately, it is the responsibility of
the investigator to determine optimal conditions. These products are tools that can be
used for interpretation of morphological findings in conjunction with other diagnostic
tests and pertinent clinical data by a qualified pathologist.
Quality Control:
Refer to NCCLS Quality Assurance for Immunocytochemistry approved guidelines,
December 1999 MM4-A Vol.19 No.26 for more information about tissue controls.
Precautions:
This antibody contains less than 0.1% sodium azide. Concentrations less than 0.1% are
not reportable hazardous materials according to U.S. 29 CFR 1910.1200, OSHA
Hazard communication and EC Directive 91/155/EC.
Sodium azide (NaN3) used as a preservative is toxic if ingested. Sodium azide may
react with lead and copper plumbing to form highly explosive metal azides. Upon
disposal, flush with large volumes of water to prevent azide build-up in plumbing.
(Center for Disease Control, 1976, National Institute of Occupational Safety and
Health, 1976)
Specimens, before and after fixation, and all materials exposed to them should be
handled as if capable of transmitting infection and disposed of with proper precautions.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes
with reagents and specimens. If reagents or specimens come in contact with sensitive
areas, wash with copious amounts of water.
Microbial contamination of reagents may result in an increase in nonspecific staining.
Incubation times or temperatures other than those specified may give erroneous results.
The user must validate any such change. The MSDS is available upon request.
Page 1 of 2

Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are
stored under conditions other than those specified in the package insert, they must be
verified by the user. Diluted reagents should be used promptly; any remaining reagent
should be stored at 2ºC to 8ºC.
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If atypical results occur, contact Biocare's Technical Support at
1-800-542-2002.
Limitations and Warranty:
There are no warranties, expressed or implied, which extend beyond this description.
Biocare is not liable for property damage, personal injury, or economic loss caused by
this product.
References:
1. Tacha D, Yu C, Haas T. TTF-1, Napsin A, p63, TRIM29, Desmoglein-3 and CK5:
An Evaluation of Sensitivity and Specificity and Correlation of Tumor Grade for Lung
Squamous Cell Carcinoma vs. Lung Adenocarcinoma. Modern Pathology; Abstract,
USCAP, 2011
2. Tacha D, Zhou D, Henshall-Powell RL¹. Distinguishing Adenocarcinoma from
Squamous Cell Carcinoma in Lung Using Double Stains p63+ CK5 and TTF-1 +
Napsin A. Modern Pathology; Pathology Volume 23, Supplement 1, Feb 2010;
Abstract 1852, page 222A.
3. Terry J, et al. Optimal immunohistochemical markers for distinguishing lung
adenocarcinomas from squamous cell carcinomas in small tumor samples. Am J Surg
Pathol. 2010 Dec; 34(12):1805-11.
4. Savci-Heijink CD, et al. The role of desmoglein-3 in the diagnosis of squamous cell
carcinoma of the lung. Am J Pathol. 2009 May; 174(5):1629-37. Epub 2009 Mar 26.
5. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 "Decontamination of Laboratory Sink Drains to Remove
Azide Salts."
6. National Committee for Clinical Laboratory Standards (NCCLS). Protection of
laboratory workers from infectious diseases transmitted by blood and tissue; proposed
guideline. Villanova, PA 1991;7(9). Order code M29-P.
Desmoglein 3 + Napsin A Cocktail
Prediluted Multiplex Cocktail (4-Step)
Control Number: 901-428DS-021611
ISO
9001:2000
CERTIFIED
Page 2 of 2

p63 + TRIM29 Cocktail (SqCC)
Prediluted Multiplex Cocktail (4-Step)
Control Number: 901-427DS-021611
ISO
9001:2000
CERTIFIED
Catalog Number:
Description:
Dilution:
Diluent:
PPM 427DS AA
6.0 ml, prediluted
Ready-to-use
N/A
Intended Use:
For In Vitro Diagnostic Use
Summary and Explanation:
Tumor protein p63, also known as transformation-related protein 63 is a protein that in
humans is encoded by the TP63 gene. Many studies have shown that p63 is a sensitive
(90%) and fairly specific marker for squamous cell carcinoma and may be used in
distinguishing poorly differentiated squamous cell carcinomas from adenocarcinomas.
p63 has been shown to mark approximately 5 to 10% of lung adenocarcinomas.
Tripartite motif-containing 29 (TRIM29) is a relatively new marker. A comprehensive
study has shown that TRIM29 is a sensitive marker (93.7%) for lung squamous cell
carcinoma (SqCC) and is a fairly specific marker staining only 6.1% of lung
adenocarcinomas.
p63 is a nuclear stain that marks lung SqCC (DAB), and TRIM29 is a
cytoplasmic/membrane stain that also marks lung SqCC (Fast Red). In most cases, a
co-expression of both antibodies will be observed in lung SqCC. Studies have also
shown that when p63 and/or TRIM29 is expressed in lung SqCC, a 95.4% sensitivity
and 100% specificity was achieved, if Napsin A and TTF-1 were both negative in the
same case. Therefore, the antibody cocktail of p63 + TRIM29 is an excellent screener
for discriminating lung SqCC vs. lung adenocarcinoma.
Principle of Multiplex Staining:
A Multiplex IHC stain can be accomplished in four major steps. The initial step
consists of an antibody cocktail with at least one mouse and one rabbit antibody. This
cocktail is applied to the tissue and will bind with two or more target antigens. A
multiplex detection cocktail of horseradish peroxidase (HRP) and alkaline phosphatase
(AP) conjugated secondary antibodies is applied. The third step consists of the addition
of DAB-Substrate that binds to the HRP and produces a brown chromogenic reaction
product. The fourth step consists of a Fast Red-Substrate that binds to the AP and
produces a red chromogenic reaction product.
Source: Mouse monoclonal and Rabbit polyclonal
Species Reactivity: Human, others not tested
Clone: BC4A4 + N/A
Isotype: IgG2a/kappa + Rabbit IgG
Epitope/Antigen: p63 + TRIM29
Cellular Localization:
p63 (Nuclear): Brown
TRIM29 (Cytoplasmic & membrane): Red
Positive Control: Lung squamous cell carcinoma
Normal Tissue: Prostate, bladder (p63); prostate, placenta (TRIM29)
Abnormal Tissue: Lung squamous cell carcinoma
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Supplied As: Buffer with protein carrier and preservative.
Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are
stored under conditions other than those specified in the package insert, they must be
verified by the user. Diluted reagents should be used promptly; any remaining reagent
should be stored at 2ºC to 8ºC.
Protocol Recommendations
Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment Solution (recommended): Diva
Pretreatment Protocol:
Heat Retrieval Method:
Retrieve sections using Biocare’s Decloaking Chamber at 125°C for 30 seconds. Allow
solution to cool for 10 minutes then wash in distilled water.
Protein Block:
Incubate for 10 minutes at RT with Biocare's Background Punisher.
Primary Antibody:
Incubate for 30 minutes at RT.
Double Stain Detection:
Incubate for 30 minutes at RT using Biocare's MACH 2 Double Stain 2.
Chromogen (1): Incubate for 5 minutes at RT with Biocare's Betazoid DAB.
Chromogen (2):
Incubate for 5-7 minutes at RT with Biocare's Warp Red. Rinse in deionized water.
Counterstain:
Counterstain with Hematoxylin. Rinse with deionized water. Apply Tacha's Bluing
solution for 1 minute. Rinse with deionized water.
Technical Note:
This antibody has been standardized with Biocare's MACH 2 Double Stain 2. It can
also be used on an automated staining system. Use TBS buffer for washing steps.
Performance Characteristics:
The optimum antibody dilution and protocols for a specific application can vary. These
include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue
section thickness and detection kit used. Due to the superior sensitivity of these unique
reagents, the recommended incubation times and titers listed are not applicable to other
detection systems, as results may vary. The data sheet recommendations and protocols
are based on exclusive use of Biocare products. Ultimately, it is the responsibility of
the investigator to determine optimal conditions. These products are tools that can be
used for interpretation of morphological findings in conjunction with other diagnostic
tests and pertinent clinical data by a qualified pathologist.
Quality Control:
Refer to NCCLS Quality Assurance for Immunocytochemistry approved guidelines,
December 1999 MM4-A Vol.19 No.26 for more information about tissue controls.
Precautions:
This antibody contains less than 0.1% sodium azide. Concentrations less than 0.1% are
not reportable hazardous materials according to U.S. 29 CFR 1910.1200, OSHA
Hazard communication and EC Directive 91/155/EC.
Sodium azide (NaN3) used as a preservative is toxic if ingested. Sodium azide may
react with lead and copper plumbing to form highly explosive metal azides. Upon
disposal, flush with large volumes of water to prevent azide build-up in plumbing.
(Center for Disease Control, 1976, National Institute of Occupational Safety and
Health, 1976)
Specimens, before and after fixation, and all materials exposed to them should be
handled as if capable of transmitting infection and disposed of with proper precautions.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes
with reagents and specimens. If reagents or specimens come in contact with sensitive
areas, wash with copious amounts of water.
Microbial contamination of reagents may result in an increase in nonspecific staining.
Incubation times or temperatures other than those specified may give erroneous results.
The user must validate any such change. The MSDS is available upon request.
Page 1 of 2

p63 + TRIM29 Cocktail (SqCC)
Prediluted Multiplex Cocktail (4-Step)
Control Number: 901-427DS-021611
ISO
9001:2000
CERTIFIED
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If atypical results occur, contact Biocare's Technical Support at 1-800-542
-2002.
Limitations and Warranty:
There are no warranties, expressed or implied, which extend beyond this description.
Biocare is not liable for property damage, personal injury, or economic loss caused by
this product.
References:
1. Tacha D, Yu C, Haas T. TTF-1, Napsin A, p63, TRIM29, Desmoglein-3 and CK5:
An Evaluation of Sensitivity and Specificity and Correlation of Tumor Grade for Lung
Squamous Cell Carcinoma vs. Lung Adenocarcinoma. Modern Pathology; USCAP,
2011 (Abstract Accepted)
2. Tacha D, Zhou D, Henshall-Powell RL¹. Distinguishing Adenocarcinoma from
Squamous Cell Carcinoma in Lung Using Double Stains p63+ CK5 and TTF-1 +
Napsin A. Modern Pathology; Pathology Volume 23, Supplement 1, Feb 2010;
Abstract 1852, page 222A.
3. Terry J, et al. Optimal immunohistochemical markers for distinguishing lung
adenocarcinomas from squamous cell carcinomas in small tumor samples. Am J Surg
Pathol. 2010 Dec; 34(12):1805-11.
4. Ring BZ, et al. A novel five-antibody immunohistochemical test for subclassification
of lung carcinoma. Mod Pathol. 2009 Aug; 22(8):1032-43. Epub 2009 May 8.
5. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 "Decontamination of Laboratory Sink Drains to Remove
Azide Salts."
6. National Committee for Clinical Laboratory Standards (NCCLS). Protection of
laboratory workers from infectious diseases transmitted by blood and tissue; proposed
guideline. Villanova, PA 1991;7(9). Order code M29-P.
Page 2 of 2

TTF-1 + CK5 Cocktail
Prediluted Multiplex Cocktail (4-Step)
Control Number: 901-425DS-021611
ISO
9001:2000
CERTIFIED
Catalog Number:
Description:
Dilution:
Diluent:
PM 425DS AA
6.0 ml, prediluted
Ready-to-use
N/A
Intended Use:
For In Vitro Diagnostic Use
Summary and Explanation:
Thyroid transcription factor-1 (TTF-1) is a member of the NKX2 family of
homeodomain transcription factors. It is expressed in epithelial cells of the thyroid
gland and lung. TTF-1 has been shown to be a sensitive (65-81%) and specific marker
(94%) in the majority of primary lung adenocarcinomas. Studies have shown that TTF
-1, used in combination with Napsin A, provided 93% sensitivity and 100% specificity
for lung adenocarcinoma, if CK5 and Desmoglein 3 were both negative in the same
case.
CK5 is a type II intermediate filament protein that is expressed in active basal layers of
most stratified squamous epithelia. In a published study, rabbit monoclonal CK5
antibody was compared to mouse monoclonal CK5/6. CK5 was 84% sensitive and
100% specific for lung squamous cell carcinoma (SqCC) when compared to CK5/6
(80% sensitivity and 97% specificity). CK6 mRNA has been detected in lung
adenocarcinomas; thus CK5 alone, may be a more specific marker than CK5/6. Studies
have shown that CK5, used in combination with Desmoglein 3, provided 93.7%
sensitivity with 100% specificity for lung SqCC.
TTF-1 (lung adenocarcinoma) is stained with DAB chromogen, and CK5 rabbit
monoclonal (lung SqCC) is stained with a Fast Red chromogen. In most lung cancers
tested, only a single antibody stain will be observed. Co-expressions of both antibodies
may be an indication of adenosquamous cell carcinomas. When used in combination
with Desmoglein 3 and Napsin A, a 93% staining sensitivity and 100% specificity was
achieved for lung adenocarcinoma, and a 93.7% sensitivity and 100% specificity was
achieved for lung SqCC; therefore, the antibody cocktail of TTF-1 + CK5 is a first
class screener for discriminating between lung adenocarcinoma (TTF-1) vs. lung SqCC
(CK5).
Principle of Multiplex Staining:
A Multiplex IHC stain can be accomplished in four major steps. The initial step
consists of an antibody cocktail with at least one mouse and one rabbit antibody. This
cocktail is applied to the tissue and will bind with two or more target antigens. A
multiplex detection cocktail of horseradish peroxidase (HRP) and alkaline phosphatase
(AP) conjugated secondary antibodies is applied. The third step consists of the addition
of DAB-Substrate that binds to the HRP and produces a brown chromogenic reaction
product. The fourth step consists of a Fast Red-Substrate that binds to the AP and
produces a red chromogenic reaction product.
Source: Mouse monoclonal and Rabbit monoclonal
Species Reactivity: Human, others not tested
Clone: 8G7G3/1 + EP1601Y
Isotype: IgG1 + Rabbit IgG
Epitope/Antigen: TTF-1 + CK5
Cellular Localization:
TTF-1 (Nuclear): Brown
CK5 (Cytoplasmic): Red
Positive Control: Lung adenocarcinoma (TTF-1) and lung SqCC (CK5)
Normal Tissue: Lung (TTF-1); normal prostate (CK5)
Abnormal Tissue: Lung adenocarcinoma (TTF-1); lung SqCC (CK5)
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Supplied As: Buffer with protein carrier and preservative.
Protocol Recommendations
Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment Solution (recommended): Diva
Pretreatment Protocol:
Heat Retrieval Method:
Retrieve sections using Biocare’s Decloaking Chamber at 125°C for 30 seconds. Allow
solution to cool for 10 minutes then wash in distilled water.
Protein Block:
Incubate for 10 minutes at RT with Biocare's Background Punisher.
Primary Antibody:
Incubate for 30 minutes at RT.
Double Stain Detection:
Incubate for 30 minutes at RT using Biocare's MACH 2 Double Stain 2.
Chromogen (1): Incubate for 5 minutes at RT with Biocare's Betazoid DAB.
Chromogen (2):
Incubate for 5-7 minutes at RT with Biocare's Warp Red. Rinse in deionized water.
Counterstain:
Counterstain with Hematoxylin. Rinse with deionized water. Apply Tacha's Bluing
solution for 1 minute. Rinse with deionized water.
Technical Note:
This antibody has been standardized with Biocare's MACH 2 Double Stain 2. It can
also be used on an automated staining system. Use TBS buffer for washing steps.
Performance Characteristics:
The optimum antibody dilution and protocols for a specific application can vary. These
include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue
section thickness and detection kit used. Due to the superior sensitivity of these unique
reagents, the recommended incubation times and titers listed are not applicable to other
detection systems, as results may vary. The data sheet recommendations and protocols
are based on exclusive use of Biocare products. Ultimately, it is the responsibility of
the investigator to determine optimal conditions. These products are tools that can be
used for interpretation of morphological findings in conjunction with other diagnostic
tests and pertinent clinical data by a qualified pathologist.
Quality Control:
Refer to NCCLS Quality Assurance for Immunocytochemistry approved guidelines,
December 1999 MM4-A Vol.19 No.26 for more information about tissue controls.
Precautions:
This antibody contains less than 0.1% sodium azide. Concentrations less than 0.1% are
not reportable hazardous materials according to U.S. 29 CFR 1910.1200, OSHA
Hazard communication and EC Directive 91/155/EC.
Sodium azide (NaN3) used as a preservative is toxic if ingested. Sodium azide may
react with lead and copper plumbing to form highly explosive metal azides. Upon
disposal, flush with large volumes of water to prevent azide build-up in plumbing.
(Center for Disease Control, 1976, National Institute of Occupational Safety and
Health, 1976)
Specimens, before and after fixation, and all materials exposed to them should be
handled as if capable of transmitting infection and disposed of with proper precautions.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes
with reagents and specimens. If reagents or specimens come in contact with sensitive
areas, wash with copious amounts of water.
Microbial contamination of reagents may result in an increase in nonspecific staining.
Incubation times or temperatures other than those specified may give erroneous results.
The user must validate any such change. The MSDS is available upon request.
Page 1 of 2

TTF-1 + CK5 Cocktail
Prediluted Multiplex Cocktail (4-Step)
Control Number: 901-425DS-021611
ISO
9001:2000
CERTIFIED
Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are
stored under conditions other than those specified in the package insert, they must be
verified by the user. Diluted reagents should be used promptly; any remaining reagent
should be stored at 2ºC to 8ºC.
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If atypical results occur, contact Biocare's Technical Support at
1-800-542-2002.
Limitations and Warranty:
There are no warranties, expressed or implied, which extend beyond this description.
Biocare is not liable for property damage, personal injury, or economic loss caused by
this product.
References:
1. Mukhopadhyay S, Katzenstein AL. Subclassification of non-small cell lung
carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an
immunohistochemical panel containing TTF-1, napsin A, p63, and CK5/6. Am J Surg
Pathol. 2011 Jan; 35(1):15-25.
2. Tacha D, Yu C, Haas T. TTF-1, Napsin A, p63, TRIM29, Desmoglein-3 and CK5:
An Evaluation of Sensitivity and Specificity and Correlation of Tumor Grade for Lung
Squamous Cell Carcinoma vs. Lung Adenocarcinoma. Modern Pathology; USCAP,
2011 (Abstract Accepted)
3. Tacha D, Zhou D, Henshall-Powell RL¹. Distinguishing Adenocarcinoma from
Squamous Cell Carcinoma in Lung Using Double Stains p63+ CK5 and TTF-1 +
Napsin A. Modern Pathology; Pathology Volume 23, Supplement 1, Feb 2010;
Abstract 1852, page 222A.
4. Terry J, et al. Optimal immunohistochemical markers for distinguishing lung
adenocarcinomas from squamous cell carcinomas in small tumor samples. Am J Surg
Pathol. 2010 Dec; 34(12):1805-11.
5. Kargi A, Gurel D, Tuna B. The diagnostic value of TTF-1, CK 5/6, and p63
immunostaining in classification of lung carcinomas. Appl Immunohistochem Mol
Morphol. 2007 Dec; 15(4):415-20.
6. Downey P, et al. If it's not CK5/6 positive, TTF-1 negative it's not a squamous cell
carcinoma of lung. APMIS 2008 Jun; 116(6):526-9.
7. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 "Decontamination of Laboratory Sink Drains to Remove
Azide Salts."
8. National Committee for Clinical Laboratory Standards (NCCLS). Protection of
laboratory workers from infectious diseases transmitted by blood and tissue; proposed
guideline. Villanova, PA 1991;7(9). Order code M29-P.
Page 2 of 2