COMPARATIVE PATHOLOGY LABORATORY - University of ...
COMPARATIVE PATHOLOGY LABORATORY - University of ...
COMPARATIVE PATHOLOGY LABORATORY - University of ...
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<strong>COMPARATIVE</strong> <strong>PATHOLOGY</strong> <strong>LABORATORY</strong><br />
DEPARTMENT OF <strong>PATHOLOGY</strong> • COLLEGE OF VETERINARY MEDICINE • UNIVERSITY OF GEORGIA<br />
501 DW BROOKS DRIVE ATHENS, GA 30602 • TEL.: 706-202-8608 • F AX: 706-542-5828<br />
E-MAIL: TNAGY@UGA.EDU<br />
WEB: WWW.VET.UGA.EDU/VPP/SERVICES/LABS/COMPPATH.PHP<br />
RESEARCH <strong>PATHOLOGY</strong> SERVICES<br />
• Gross pathology, histopathology,<br />
Immunohistochemistry, electron microscopy, and<br />
clinical pathology services<br />
• Phenotyping <strong>of</strong> genetically modified animals<br />
• Genetically modified mouse lines available for cancer<br />
research (inquire)<br />
• Preparation <strong>of</strong> publication quality digital images with<br />
caption and legend<br />
• Novel drug delivery technologies<br />
• Ante‐mortem imaging studies (X‐ray and MRI)<br />
• Infectious disease surveillance for rodents and<br />
primates<br />
• Assistance with grant proposals<br />
• Video conferencing with real‐time histological images<br />
TAMAS NAGY, DVM, PHD<br />
Assistant Pr<strong>of</strong>essor and Director<br />
Comparative Pathology Laboratory<br />
Education<br />
1994: Doctor <strong>of</strong> Veterinary Medicine<br />
<strong>University</strong> <strong>of</strong> Veterinary Science Budapest, Hungary<br />
1995-1997: Postdoctoral Research<br />
Yale <strong>University</strong> School <strong>of</strong> Medicine, New Haven, CT<br />
1997-2000: Veterinary Pathology Residency<br />
Cornell <strong>University</strong>, Ithaca, NY<br />
2007: Doctor <strong>of</strong> Philosophy<br />
Cornell <strong>University</strong>, Ithaca, NY<br />
SAMPLE PRICE LIST ‐ 2011<br />
Service Unit Unit price<br />
Necropsy procedure, mouse Mouse cadaver $75.00<br />
Necropsy procedure, other species Cadaver Inquire<br />
Histopathological/Cytological evaluation (1‐4 tissue sections per slide) Glass slide $65.00<br />
Histopathological evaluation (>4 tissue sections per slide) Tissue section $15.00<br />
Publication quality photomicrographs Digital image $25.00<br />
Preparation <strong>of</strong> caption and legend for photomicrographs Digital image $40.00<br />
Immunohistochemistry optimization for new antibody (procedure,<br />
interpretation, and report) Antibody $250.00<br />
Immunohistochemistry with antibodies in use in CPL (inquire about antibody<br />
list) Glass slide $40.00<br />
CBC with interpretation (300 µl EDTA‐treated whole blood) Blood sample $45.00<br />
Serum chemistry, Liver panel (400 µl serum)<br />
Albumin, Total protein, Globulin, Alb:Glob, ALP, ALT, AST, GGT, Bile acids, Cholesterol Serum sample $85.00<br />
Serum Chemistry, Renal panel (400 µl serum)<br />
Albumin, Total protein, Globulin, BUN, Ca, Cl, K, Na, Bicarbonate, Creatinine,<br />
Phosphorus Serum sample $75.00<br />
Serum chemistry, Muscle panel (400 µl serum)<br />
ALT, AST, Creatine kinase, LDH Serum sample $50.00<br />
Serum chemistry, Lipid panel (400 µl serum)<br />
Cholesterol & triglycerides Serum sample $45.00<br />
Bone marrow smear (along with 300 µl EDTA‐treated whole blood) Bone marrow smear $85.00<br />
Coagulation pr<strong>of</strong>ile (300 µl whole blood in citrate) Blood sample $55.00<br />
Urinalysis (with sediment analysis)<br />
Urine creatinine, Urine electrolytes (Na + /K + /HCO3 ‐ ), Electrolyte:Creatinine<br />
Protein Creatine<br />
Urine sample $45.00<br />
Phone: 706-202-8607 • E-mail: TNAGY@UGA.EDU
Comparative Pathology Laboratory<br />
Department <strong>of</strong> Pathology • College <strong>of</strong> Veterinary Medicine • <strong>University</strong> <strong>of</strong> Georgia<br />
Special Stains Available<br />
Staining procedure<br />
Specific for<br />
Acid Fast<br />
Mycobacteria<br />
Alcian Blue<br />
Mucin<br />
Bielchowsky's<br />
Axons<br />
Bodian's<br />
Axons<br />
Congo Red<br />
Amyloid<br />
Copper<br />
Stored Copper in tissues<br />
Feulgen reaction<br />
DNA<br />
Fraser Lendrum<br />
Fibrin<br />
Fontana Masson<br />
Melanin<br />
Giemsa<br />
Blood film, Histoplasma capsulatum , Mast cell granules<br />
Gimenez<br />
Gram positive and Gram negative bacteria<br />
GMS<br />
Fungus<br />
Gomori's reticulum<br />
Reticular fibers<br />
Gomori's trichrome<br />
Myopathic changes (nemaline rods, ragged red fibers)<br />
Grimelius<br />
Argyrophil granules in neurosecretory tumors<br />
Hall's<br />
Bile in tissue sections<br />
Holmes<br />
Nerve fibers and neur<strong>of</strong>ibrils<br />
Holzer<br />
Glial fibers in areas <strong>of</strong> gliosis<br />
Lillie Twort<br />
Gram positive and Gram negative bacteria<br />
Luna<br />
Elastic fibers and Mast cells<br />
Luxol Fast Blue<br />
Myelin<br />
Masson's trichrome<br />
Collagen<br />
Mayer's mucicarmine<br />
carmine Mucin<br />
Melanin bleach<br />
Cellular details in heavily pigmented tissues sections<br />
Methyl Green Pyronin Y DNA is green and RNA is red with pyronin<br />
Movat's Pentachrome<br />
Elastic fibers (purple), Mucin (blue), and Collagen (yellow)<br />
Oil Red O<br />
Fat<br />
Okajima<br />
Hemoglobin<br />
PAM (Periodic acid silver‐methenamine)<br />
Glomerular basement membrane<br />
PAS (Periodic acid‐Schiff)<br />
Glycoprotein macromolecules<br />
PAS (Periodic acid‐Schiff) with and without diastase Glycogen<br />
Perl's iron<br />
Iron in tissue sections<br />
PTAH (Phosphotungstic acid haematoxylin)<br />
Fibrin<br />
Safranin O<br />
Nuclei, Mucin, Mast cell granules<br />
Schmorl's<br />
Melanin<br />
Sudan IV<br />
Fat<br />
Toluidin Blue<br />
DNA (blue) and sulfated polysaccharides (purple)<br />
Verhoef van Gieson<br />
Elastic fibers<br />
von Kossa<br />
Calcium deposits<br />
Warthin Starry<br />
Certain bacteria (Leptospira sp., Helicobacter sp., etc.)<br />
Phone: 706‐202‐8608<br />
E‐mail: TNAGY@UGA.EDU<br />
Tamas Nagy, DVM, PhD<br />
501 DW BROOKS DRIVE ATHENS, GA 30602 •TEL.: 706‐202‐8608 •FAX: 706‐542‐5828<br />
E‐MAIL: TNAGY@UGA.EDU<br />
WEB: WWW.VET.UGA.EDU/VPP/SERVICES/LABS/COMPPATH.PHP
Comparative Pathology Laboratory<br />
Department <strong>of</strong> Pathology • College <strong>of</strong> Veterinary Medicine • <strong>University</strong> <strong>of</strong> Georgia<br />
Antibodies Antibody source Selected examples <strong>of</strong> use<br />
Lymphoid markers<br />
BLA36 Mouse Lympoid organ development/Classification <strong>of</strong> lymphoproliferative disorders<br />
CD3 Rabbit Lympoid organ development/Classification <strong>of</strong> lymphoproliferative disorders<br />
CD18 Mouse Lympoid organ development/Classification <strong>of</strong> lymphoproliferative disorders<br />
CD21 Mouse Lympoid organ development/Classification <strong>of</strong> lymphoproliferative disorders<br />
CD79 Mouse Lympoid organ development/Classification <strong>of</strong> lymphoproliferative disorders<br />
Endocrine markers<br />
Calcitonin Rabbit Identification <strong>of</strong> C‐cells (parafollicular cells)<br />
Thyroglobulin Rabbit Identification <strong>of</strong> follicular epithelial cells<br />
Glucagon Rabbit Differentiation marker for stem cells, etc.<br />
Insulin Guinea pig Differentiation marker for stem cells, etc.<br />
Proliferation markers<br />
Ki‐67 Rabbit Determining proliferative index <strong>of</strong> tumors<br />
PCNA Mouse Determining proliferative index <strong>of</strong> tumors<br />
Apoptosis markers<br />
Apoptag kit Rabbit Determining apoptotic index <strong>of</strong> tumors<br />
Intermediate filaments<br />
Cytokeratin, Pan Mouse Teratoma analysis, differentiation marker for stem cells, etc.<br />
Cytokeratin, AE1/AE3 Mouse Teratoma analysis, differentiation marker for stem cells, etc.<br />
Neur<strong>of</strong>ilament Mouse Teratoma analysis, differentiation marker for stem cells, etc.<br />
GFAP Mouse Teratoma analysis, differentiation marker for stem cells, etc.<br />
Vimentin Mouse Epithelial‐mesenchymal transition, teratoma analysis, etc.<br />
Cell‐specific proteins<br />
Desmin Mouse Specific for all types <strong>of</strong> muscle cells<br />
Smooth muscle actin Mouse Specific for smooth muscle cells (myoepithelial cells, etc .<br />
)<br />
Lysozyme Rabbit Specific marker for tissue macrophages<br />
Mac387 Mouse Specific marker for tissue macrophages<br />
Factor VII‐related antigen Rabbit Specific marker for endothelial cells<br />
Myelin Basic Protein Mouse Specific marker for myelin<br />
Neuron Specific Enolase Mouse Specific marker for neurons (teratoma analysis, etc.)<br />
S100 Rabbit Specific marker for Schwann cells <strong>of</strong> the PNS<br />
Melan A Mouse Specific marker for melanin‐producing cells<br />
Miscellaneous proteins<br />
C‐kit Rabbit Proto‐oncogene (tumor prognosis, cell signaling, ect.)<br />
Chromogranin A Mouse Marker for neurons and endocrine cells<br />
Synaptophysin Mouse Specific for neuroendocrine cells and neurons in CNS<br />
COX‐2 Mouse Identification <strong>of</strong> COX‐2 (over)expression<br />
E‐Cadherin Mouse Epithelial‐specific (epitheilal‐mesencymal transition, etc.)<br />
p53 Mouse Tumor suppressor gene (tumor diagnosis, etc.)<br />
Wilms' Tumor Antigen Rabbit Proto‐oncogene (tumor prognosis, cell signaling, ect.)<br />
Phone: 706‐202‐8608<br />
E‐mail: TNAGY@UGA.EDU<br />
Tamas Nagy, DVM, PhD<br />
501 DW BROOKS DRIVE ATHENS, GA 30602 •TEL.: 706‐202‐8608 •FAX: 706‐542‐5828<br />
E‐MAIL: TNAGY@UGA.EDU<br />
WEB: WWW.VET.UGA.EDU/VPP/SERVICES/LABS/COMPPATH.PHP
Comparative Pathology Laboratory<br />
Department <strong>of</strong> Pathology • College <strong>of</strong> Veterinary Medicine • <strong>University</strong> <strong>of</strong> Georgia<br />
Viral agents ‐ species specific<br />
Bovine Herpesvirus<br />
Bovine Respiratory Syncytial Virus<br />
Bovine Viral Diarrhea Virus<br />
Canine Adenovirus Type I<br />
Canine Distemper Virus<br />
Coronavirus Group I (FIP, TGE, CCV)<br />
Coronavirus Group II (Bovine, Elk)<br />
Eastern Equine Encephalitis Virus<br />
Epizootic Hemorrhagic Disease Virus <strong>of</strong> Deer<br />
Parvovirus (Canine and Feline)<br />
Vesicular Stomatitis Virus<br />
Viral agents ‐ non‐species specific<br />
Influenza A Virus<br />
Papillomavirus<br />
Rabies Virus<br />
West Nile Virus<br />
Antibodies to identify various infectious agents<br />
Bacterial agents<br />
Anaplasma sp.<br />
Bartonella henselae<br />
Borrelia burgdorferi<br />
Campylobacter sp.<br />
Chlamydia sp.<br />
Francisella tularensis<br />
Leptospira sp.<br />
Listeria monocytogenes<br />
Mycoplasma sp.<br />
Fungal agents<br />
Aspergillus sp.<br />
Blastomyces dermatitidis<br />
Coccidioides immitis<br />
Histoplasma capsulatum<br />
Protozoal agents<br />
Cryptosporidium parvum<br />
Neospora caninum<br />
Sarcocystis neurona<br />
Toxoplasma gondii<br />
Phone: 706‐202‐8608<br />
E‐mail: TNAGY@UGA.EDU<br />
Tamas Nagy, DVM, PhD<br />
501 DW BROOKS DRIVE ATHENS, GA 30602 •TEL.: 706‐202‐8608 •FAX: 706‐542‐5828<br />
E‐MAIL: TNAGY@UGA.EDU<br />
WEB: WWW.VET.UGA.EDU/VPP/SERVICES/LABS/COMPPATH.PHP
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