Synthesis, characterization and antimicrobial studies on transition ...

S. Sunitha <strong>and</strong> K.K. Aravindakshan, Int J Pharm Biomed Sci 2011, 2(4), 103-113
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Cu(II) were evaluated for in vitro antibacterial activity
against Escherichia coli <strong>and</strong> in vitro antifungal activity
against Aspergizlus niger. Nutrient Agar 1.5% [20] <strong>and</strong>
potato agar medium [21] were employed for the bacterial <strong>and</strong>
fungal growth respectively. The zones of inhibition <strong>and</strong> the
minimum inhibitory concentrations were determined <strong>and</strong>
presented in Table 4 <strong>and</strong> Fig.5(A-D). The lig<strong>and</strong> did not
show any antibacterial activity but the complexes showed
antibacterial activity.
The lig<strong>and</strong> <strong>and</strong> five of the complexes were investigated
for antifungal activity. The lig<strong>and</strong>, the iodo complex of
Co(II), acetato complex of Ni(II), acetato, chloro <strong>and</strong> nitrato
complexes of Cu(II) were tested against Aspergillus niger.
The zones of inhibition have been tabulated in Table 5 <strong>and</strong>
Fig.6(A-F). It has been observed that the lig<strong>and</strong> <strong>and</strong> all the
tested complexes showed antifungal activity against
Aspergillus niger. Except the acetato complex of Ni(II), all
the complexes showed more antifungal activity than the
lig<strong>and</strong>.
4. CONCLUSIONS
A total of seven complexes of N-phenyl(methylphenyl-5-
pyrazolyl)methylidene]aniline were synthesized <strong>and</strong>
characterised. The physico-chemical properties of all the
complexes were studied <strong>and</strong> they were found to have the
general formulae [ML 2 X 2 ]. The structures suggested for these
compounds are given in Figs.3& 4. The lig<strong>and</strong> <strong>and</strong> the
complexes were screened for their antibacterial activity
against Escherichia coli <strong>and</strong> for their antifungal activity
against Aspergillus niger.
The lig<strong>and</strong> <strong>and</strong> the complexes were screened for their
antibacterial activity against Escherichia coli. The zones of
inhibition were determined. They were also screened for their
antifungal activity against Aspergillus niger. The minimum
inhibitory concentration was determined. The lig<strong>and</strong> did not
exhibit any antibacterial activity but the complexes showed
activity against E. coli. The lig<strong>and</strong> <strong>and</strong> the complexes showed
antifungal activity.
REFERENCES
[1] Jain R, Shukla A, J Indian Chem Soc 1990, 67, 575.
[2] Akam Y, Yakota H, Sato K., Nakai T., Talanta, 1986, 33, 288.
[3] Wiley RH, Wiley P, ‘Heterocyclic compounds Pyrazolones,
Pyrazolidines <strong>and</strong> Derivatives’, Interscience, New York 1964.
[4] Goodman LS, Gilman A, The Pharmacological Basis of Therapeutics,
48th Ed., Macmillan, London 1970.
[5] Furnisss BS, Hannaford AJ, Smith PWG, Tatchell AR, Vogel’s
Textbook of Practical Organic Chemistry, Addison-Wesley Longman
Limited, Engl<strong>and</strong> 1989, pp.1150-1151.
[6] Jenson BS, Acta Chem Sc<strong>and</strong> 1959, 13, 1668.
[7] Silverstein SM, Bassler GC, Morrill TC, Spectrophotometric
Identification of Organic Compounds, John Wiley, New York 1991.
[8] Koji Nakanishi, Infrared Absorption Spectroscopu’, Holden-Day,Inc.,
Sanfrancisco 1966.
[9] Lever ABP, Inorganic Electronic Spectrosocpy, Elsevier, Amsterdam,
p.479.
[10] Aly Hassan MA, J. Islamic Acad Sci 1991, 4, 271-274.
[11] Baba S, Kawaguchi S, Inorg Nucl Chem Lett 1973, 9, 1287.
[12] Clark RJH, in: Halogen Chemistry, Gutmann V., Ed., Vol. 3, Academic
Press, NewYork 1967.
[13] Nuttal RH, Talanta 1968, 15, 157.
[14] Carty AJ, Coord Chem Rev 1969, 4, 29.
[15] Curtis NF, Curtis YM, Inorg Chem 1965, 4, 804.
[16] Barraclough CG, Tobe ML, J Chem Soc 1993, 1961
[17] Eskenazi R, Rasavan J, Levitus R, J Inorg Med Chem 1966, 28, 521.
[18] Lever ABP, Inorganic Electronic Spectroscopy, Elsevier, Amsterdam,
p.507.
[19] Rai HC, Sahoo B, J Indian Chem Soc 1976, 53, 636.
[20] Downes <strong>and</strong> Ito(ed) ‘Compendium for the microbiological examination
of foods’, American Public Health Association, 4th Ed., Washington
DC 2001.
[21] Beever RE, Bollard EG, J Gen Microbiol 1970, 60, 272-279.
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