BEI RESOURCE DEPOSIT FORM FOR - BEI Resources

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DEPOSIT FORM – Part 2 of 2 ANTIBODIES, HYBRIDOMAS & ANTISERA Age of immunized subject:______________________________________________________________ Route of immunization ( footpad, intrascapular, intrperitoneal, etc.): ______________________________ Adjuvant: ____________________________________________________________________________ Was antigen conjugated Yes □ No □ If yes, name conjugate (e.g., BSA, ovlabumin, etc.): ________________________________________ g. Screening Assay_________________________________________________________________________ ______________________________________________________________________________________ 2. ANTIBODIES (monoclonal): a. Was the antibody obtained from a human Yes □ No □ If yes, were the IRB approved consent form(s) obtained Yes □ No □ If yes again, please provide RPN Number: __________________________________________________ b. Has the antibody been purified: Yes □ No □ Unknown □ If yes, state method: ___________________________________________________________________ c. Is antibody conjugated Yes □ No □ If yes, name conjugate (e.g., fluorescein, rhodamine, alkaline phosphatase, etc.): ____________________________________________________________________________________ d. Antibody: Specificity: ___________________________________________________________________________ Cross-reactivity (if any):_________________________________________________________________ Ig Class: __________________________________ Subclass: _________________________________ Light chain: lambda □ kappa □ Unknown □ e. Special properties, characteristics, or use: Can it be used for: Western blots Yes □ No □ Unknown □ Immunoprecipitation Yes □ No □ Unknown □ Flow cytometry Yes □ No □ Unknown □ Biodefense and Emerging Infections Research Resources Repository 800-359-7370 P.O. Box 4137 Fax: 703-365-2898 Manassas, VA 20108-4137 USA E-mail: contact@beiresources.org www.beiresources.org Effective Date: 09/04/2007 Revision: 1 © 2004 American Type Culture Collection (ATCC). All rights reserved. Document ID: 4388 Page 6 of 10
DEPOSIT FORM – Part 2 of 2 ANTIBODIES, HYBRIDOMAS & ANTISERA Immunocytochemistry Yes □ No □ Unknown □ Is it neutralizing Yes □ No □ Unknown □ Can it bind to both denatured and native protein Yes □ No □ Unknown □ Can it be labeled or radio-labeled without loosing specificity Yes □ No □ Unknown □ f. Recommended titers for use in: Western blots: __________________________ Radioimmunoassays: _________________________ Immunoprecipitation: _____________________ Elisa: _____________________________________ Flow cytometry: _________________________ Neutralization assays: ________________________ Immunohistochemistry: _______________ 3. HYBRIDOMAS: a. Brief history since origin (important changes in culture conditions or methods, clonal method, if any, whether antibiotics have been used, current passage number, population doublings (PDLs), etc.): _____________________________________________________________________________________ _____________________________________________________________________________________ b. Was the hybridoma obtained from a human Yes □ No □ If yes, were the IRB approved consent form(s) obtained Yes □ No □ If yes again, please provide RPN Number: __________________________________________________ c. Hybridoma origin Species of antibody producing cell: _______________________________________________________ Was immunization conducted in vivo or in vitro _____________________________________________ Fusion partner (designation/strain/species): _________________________________________________ d. Antibody: Specificity: ___________________________________________________________________________ Cross-reactivity (if any):_________________________________________________________________ Ig Class: _______________________________________ Subclass: ____________________________ Light chain: lambda □ kappa □ Unknown □ Biodefense and Emerging Infections Research Resources Repository 800-359-7370 P.O. Box 4137 Fax: 703-365-2898 Manassas, VA 20108-4137 USA E-mail: contact@beiresources.org www.beiresources.org Effective Date: 09/04/2007 Revision: 1 © 2004 American Type Culture Collection (ATCC). All rights reserved. Document ID: 4388 Page 7 of 10
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BEI RESOURCE DEPOSIT FORM FOR - BEI Resources

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