BEI RESOURCE DEPOSIT FORM FOR - BEI Resources
BEI RESOURCE DEPOSIT FORM FOR - BEI Resources
BEI RESOURCE DEPOSIT FORM FOR - BEI Resources
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<strong>DEPOSIT</strong> <strong><strong>FOR</strong>M</strong> – Part 2 of 2<br />
ANTIBODIES, HYBRIDOMAS & ANTISERA<br />
e. Medium, antibiotics, etc. used in stock transferred to <strong>BEI</strong> <strong>Resources</strong>:________________________________<br />
________________________________________________________________________________________<br />
f. Recommended culture medium and subculture procedure: ________________________________________<br />
______________________________________________________________________________________<br />
g. Recommendations for freezing cells, if any: ____________________________________________________<br />
4. ANTISERUM:<br />
a. Dates of immunization protocol (was antibody from a primary response, secondary, etc.):<br />
_____________________________________________________________________________________<br />
b. Was antisera pooled Yes □ No □ Unknown □<br />
If yes, how many subjects contributed to pool ______________________________________________<br />
c. Has the antibody been purified: Yes □ No □ Unknown □<br />
If yes, state method: ___________________________________________________________________<br />
d. Is antibody in serum conjugated Yes □ No □<br />
If yes, name conjugate (e.g., fluorescein, rhodamine, alkaline phosphatase, etc.):<br />
____________________________________________________________________________________<br />
e. Antibody:<br />
Specificity: ___________________________________________________________________________<br />
Cross-reactivity (if any):_________________________________________________________________<br />
Ig Class: __________________________________ Subclass: _________________________________<br />
Light chain: lambda □ kappa □ Unknown □<br />
f. Special properties, characteristics, or use:<br />
Can it be used for:<br />
Western blots Yes □ No □ Unknown □<br />
Immunoprecipitation Yes □ No □ Unknown □<br />
Flow cytometry Yes □ No □ Unknown □<br />
Immunocytochemistry Yes □ No □ Unknown □<br />
Is it neutralizing Yes □ No □ Unknown □<br />
Can it bind denatured as well as native protein Yes □ No □ Unknown □<br />
Can it be labeled or radiolabeled without loosing specificity Yes □ No □ Unknown □<br />
Biodefense and Emerging Infections Research <strong>Resources</strong> Repository 800-359-7370<br />
P.O. Box 4137 Fax: 703-365-2898<br />
Manassas, VA 20108-4137 USA<br />
E-mail: contact@beiresources.org<br />
www.beiresources.org Effective Date: 09/04/2007<br />
Revision: 1<br />
© 2004 American Type Culture Collection (ATCC). All rights reserved. Document ID: 4388<br />
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