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BEI RESOURCE DEPOSIT FORM FOR - BEI Resources

BEI RESOURCE DEPOSIT FORM FOR - BEI Resources

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<strong>DEPOSIT</strong> <strong><strong>FOR</strong>M</strong> – Part 2 of 2<br />

ANTIBODIES, HYBRIDOMAS & ANTISERA<br />

e. Medium, antibiotics, etc. used in stock transferred to <strong>BEI</strong> <strong>Resources</strong>:________________________________<br />

________________________________________________________________________________________<br />

f. Recommended culture medium and subculture procedure: ________________________________________<br />

______________________________________________________________________________________<br />

g. Recommendations for freezing cells, if any: ____________________________________________________<br />

4. ANTISERUM:<br />

a. Dates of immunization protocol (was antibody from a primary response, secondary, etc.):<br />

_____________________________________________________________________________________<br />

b. Was antisera pooled Yes □ No □ Unknown □<br />

If yes, how many subjects contributed to pool ______________________________________________<br />

c. Has the antibody been purified: Yes □ No □ Unknown □<br />

If yes, state method: ___________________________________________________________________<br />

d. Is antibody in serum conjugated Yes □ No □<br />

If yes, name conjugate (e.g., fluorescein, rhodamine, alkaline phosphatase, etc.):<br />

____________________________________________________________________________________<br />

e. Antibody:<br />

Specificity: ___________________________________________________________________________<br />

Cross-reactivity (if any):_________________________________________________________________<br />

Ig Class: __________________________________ Subclass: _________________________________<br />

Light chain: lambda □ kappa □ Unknown □<br />

f. Special properties, characteristics, or use:<br />

Can it be used for:<br />

Western blots Yes □ No □ Unknown □<br />

Immunoprecipitation Yes □ No □ Unknown □<br />

Flow cytometry Yes □ No □ Unknown □<br />

Immunocytochemistry Yes □ No □ Unknown □<br />

Is it neutralizing Yes □ No □ Unknown □<br />

Can it bind denatured as well as native protein Yes □ No □ Unknown □<br />

Can it be labeled or radiolabeled without loosing specificity Yes □ No □ Unknown □<br />

Biodefense and Emerging Infections Research <strong>Resources</strong> Repository 800-359-7370<br />

P.O. Box 4137 Fax: 703-365-2898<br />

Manassas, VA 20108-4137 USA<br />

E-mail: contact@beiresources.org<br />

www.beiresources.org Effective Date: 09/04/2007<br />

Revision: 1<br />

© 2004 American Type Culture Collection (ATCC). All rights reserved. Document ID: 4388<br />

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