chemi-smart™ 5100 - Peqlab

CHEMI-SMART
Chemiluminescence-Imaging Systems
Creating the future together.

principle
Western Blot detection using
CHEMI-SMART 5100.
Chemiluminescence Detection
Chemiluminescence has become the standard method for
specific nucleic acid and protein detection. With its outstanding
sensitivity this method has largely replaced imaging procedures
using radioactive labels. Due to small target molecule quantities,
the amount of light emitted during a chemiluminescent reaction
is typically very low and its detection requires highly sensitive
and specialised optics as well as optimised signal processing
mechanisms to record even the faintest signals.
High Sensitivity
Sensitivity is the key property of a chemiluminescence system
and depends on careful selection of various hardware
components which must operate in perfect harmony. One
important determinant of sensitivity is the quantum efficiency
of the light sensor in the spectral range of chemiluminescent
substrates (QE, efficiency of the transformation of light quants
into electrical charge). Further important factors with impact
on sensitivity are light transmittance of the camera optics as
well as the distance between object and detection unit.
CHEMI-SMART is a synonym for chemiluminescence imaging
systems with unparalleled sensitivity thanks to perfect tuning
of a multitude of factors.
chemiluminescence
Low Noise Level
In order to benefit from high sensitivity it must go hand in hand
with a minimum possible degree of background noise. Otherwise
faint signals may become lost in the background rendering
their detection impossible. Background noise originates mainly
from two sources: by a thermally driven process spontaneously
releasing electrons from conductor material and during readout
of the CCD light sensor. CHEMI-SMART systems are characterised
by an extraordinary low system noise level achieved by
an exclusive double cooling system and continuously optimised
camera electronics and readout mechanisms.
Image Integrity without Compromise
It is our claim to provide you with qualitative excellent image
data representing a 100 % image of reality. Scientific statements
should be made on the basis of unmodified raw data. The
decision on type and degree of image modification and data
processing should at any time be the user's. The design of our
chemiluminescence imaging systems is in precise concordance
with this principle.

ultimate
CHEMI-SMART 5100
CHEMI-SMART 5100 represents chemiluminescent imaging of the highest quality. The
system represents the product of extensive practical experience in imaging technology,
continuous development and uncompromising standards in the choice and tuning of its
components.
In vivo Luciferin Bioluminescence detection using
CHEMI-SMART 5100.
CHEMI-SMART 5100 – The Ultimate Sensitivity
ß Dedicated high performance chemiluminescence imaging
system
ß Outstanding sensitivity in the emission range of
chemiluminescent substrates (QE 450nm
: 70 %)
ß Most precise signal quantifications thanks to true 16 bits
imaging (65,536 grey levels)
ß Maximum light transmittance: brightest fixed angle lens
(f 0.95) and minimum object distance (< 20 cm)
ß Wide dynamic range of 4.8 orders of magnitude for the
documentation of strong and faint signals in the same image
ß Lowest noise level through exclusive double cooling system
and optimised readout mechanisms
ß Absolute image integrity, unmodified raw image data for
scientific analyses
ß Tray-system with 6 fixed positions for different size objects
ß Included imaging software with innovative image acquisition
modes for excellent results in a single attempt
sensitivity
3
4

FUSION FX7
FUSION FX7T is a multi imaging instrument designed for maximum flexibility in imaging applications. With its high sensitivity,
it is perfectly suited for chemiluminescence detection. Additionally, it offers a wide range of fluorescence excitation and detection
possibilities.
applications
FUSION FX7 – Versatile & Powerful
ß Versatile in applications: Nucleic acid and protein
determination with all common chemiluminescence substrates
and fluorescence dyes on a variety of matrices
ß Multiple fluorescence excitation modes: UV-transillumination,
overhead UV illumination or STARLIGHT overhead
illumination
ß Outstanding sensitivity in the emission range of
chemiluminescent substrates (QE 450nm
: 70 %)
ß Most precise signal quantifications thanks to true 16 bits
Imaging (65,536 grey levels)
ß Wide dynamic range of 4.8 orders of magnitude for the
documentation of strong and faint signals in the same image
ß Lowest noise level through exclusive double cooling system and
optimised readout mechanisms
ß Brightest motorised zoom lens with autofocus and motorised
filter wheel
ß Absolute image integrity, unmodified raw image data for
scientific analyses
ß Included imaging software with innovative fluorescence and
chemiluminescence image acquisition modes for excellent
results in a single attempt and multiplex analyses
multiple
Creating the future together.

innovative
BIO-1D Advanced
BIO-1D Advanced image analysis software is both intuitive to operate and clearly structured to
work with giving outstanding functionality and innovative display options when working with
chemiluminescence, bioluminescence and fluorescence images.
• Qualitative and quantitative analysis of DNA/RNA and
protein gels as well as blots, microtitration plates, freeform
objects, agar plates and cell culture dishes
• Multiple analysis options
• Cutting edge analysis algorithms
• 'One Button Analysis' by applying analysis templates
• All tables exportable to Excel, all images and graphics can be
saved as bmp files
• Automatic menu navigation with the 'Next Tutorial Mode'
• Unique '3D Power System ' for 3 dimensional image
transformation (see adjacent figure)
• Save your analysis at any time to continue later
concepts
STARLIGHT Overhead Illumination
STARLIGHT stands for infinite possibilities of fluorescence excitation. Using high intensity LEDs a
multitude of fluorescence dyes in the red, green and blue spectral range on various, intranslucent
carrier matrices are effectively excited.
• Strong LED excitation
• Excitation maxima at 470, 528 and
624 nm
• Fluorescence detection on intranslucent
carrier matrices
• Ideally suited for multiplex imaging
analysis
• Compatible with CHEMI-SMART 3100
and 2100
Multiplex Western Blot protein detection using Qdot 565, Qdot 655
and Qdot 705 dyes. Images were generated using a CHEMI-SMART
3100 system.

Our Service Pledge
ß Product demos, system installation and on-the-spot instruction
ß Personal and professional support through our application hotline and local
field representatives
Ordering Information
Item Description Product Code
CHEMI-SMART 5100 Ultrasensitive dedicated high performance chemiluminescence imaging system with 60-CS5
'scientific grade' Firewire ® CCD camera, brightest fixed lens, unique Peltier based
double cooling system (-20 °C absolute) and tray system for precise positioning of
the imaging sample
FUSION FX7 Versatile high sensitivity multi-imaging chemiluminescence/fluorescence system with 60-FU-20M O
'scientific grade' Firewire ® CCD camera, brightest motorised zoom lens with autofocus 60-FU-26MS*
and 'lens feedback control', unique Peltier based double cooling system (-42 °C absolute), 60-FUUV-26MX**
UV transilluminator (21 x 26 cm illumination area), overhead UV illumination
(254 nm, 365 nm switchable) and 5 positions filter wheel
BIO-1D Advanced Imaging software for sophisticated analyses with wide functional range 60-BIO-1D
and unique 3D display tool for the analysis of fluorescence, chemiluminescence
and bioluminescence images
STARLIGHT Illumination Optional module for overhead fluorescence excitation in the visible range (RGB) with 60-STARLIGHT
excitation maxima at 470 nm, 528 nm and 624 nm including filter set (3 filters)
UV Conversion Screens Glass screens transforming UV to white and blue light, respectively, for the 60-FC-26WL
documentation of conventionally stained protein gels (e.g. Coomassie stain, silver stain) 60-FC-26BLUE
and for gentle fluorescence excitation in the blue for maximum sample preservation
O
with standard UV transilluminator
* with SUPER-BRIGHT UV transilluminator. Contact PEQLAB for more information on SUPER-BRIGHT Illumination Technology!
** with SUPER-BRIGHT UV transilluminator and UV-Epi-Illumination (Overhead UV-light (254 nm and 365 nm) for the exitation of fluorescence labled samples).
Deutschland
PEQLAB Biotechnologie GmbH
Carl-Thiersch-Str. 2b
91052 Erlangen
Freecall: (D) 0800 - 100 20 16
Tel: +49 (0) 9131 - 610 70 20
Fax: +49 (0) 9131 - 610 70 99
e-mail: info@peqlab.de
Internet: www.peqlab.de
Österreich
PEQLAB Biotechnologie GmbH
Zweigniederlassung Linz
Hafenstr. 47-51
4020 Linz
Tel: +43 (0) 732 - 90 156 103
Fax: +43 (0) 732 - 90 156 118
e-mail: info@peqlab.at
Internet: www.peqlab.at
United Kingdom
PEQLAB Ltd.
25 Barnes Wallis Road
Fareham PO15 5TT
Freecall: (UK) 0808 - 20 21 302
Tel: +44 (0) 1489 - 889 823
Fax: +44 (0) 1489 - 660 040
e-mail: info@peqlab.co.uk
Internet: www.peqlab.co.uk
V0708
Creating the future together.