8. PFGE: Tips and Tricks to Success and Interpretation of Results for ...

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The Big Picture • DNA tests are only part of picture – isolates from same source don’t always match – isolates with same patterns don’t always have the same source (common patterns) • Results needs to be considered along with epidemiological evidence and result from environmental investigations
Acknowledgements All PulseNet participants at CDC, FDA, USDA, and in the State Public Health Laboratories The findings and conclusions in this presentation are those of the author and do not necessarily represent the views of the Centers for Disease Control and Prevention For more information please contact Centers for Disease Control and Prevention 1600 Clifton Road NE, Atlanta, GA 30333 Telephone, 1-800-CDC-INFO (232-4636)/TTY: 1-888-232-6348 E-mail: cdcinfo@cdc.gov Web: www.cdc.gov National Center for Emerging and Zoonotic Infectious Diseases Division of Foodborne, Waterborne, and Environmental Diseases
Page 1 and 2: PFGE: Tips and Tricks to Success an
Page 3 and 4: Interpretation of PFGE Data • Tec
Page 5 and 6: Reproducibility 3 isolates of Salmo
Page 7 and 8: Artifacts • Salmonella Saintpaul
Page 9 and 10: “Ghost” or “Shadow” Bands
Page 11 and 12: Washing PFGE plugs • Washes - was
Page 13 and 14: Incomplete cell lysis Undigested, g
Page 15 and 16: High background • DNA degraded
Page 17 and 18: Enzyme digestion without BSA BSA BS
Page 19 and 20: Star activity looks like incomplete
Page 21 and 22: Impact of run length on resolution
Page 23 and 24: Interpretation of PFGE Data • Tec
Page 25 and 26: Addition of a restriction site x x
Page 27: Mode of Transmission Contrasting Sh

8. PFGE: Tips and Tricks to Success and Interpretation of Results for ...

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