Single-molecule manipulation experiments of biological molecules 1

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Magnetic Tweezers • A force is exerted on a magnetic microsphere using an applied B field • Single molecule attached between bead and surface • Bead is free to rotate, torque can be applied by rotating the B field • Force measured by imaging thermal fluctuations in bead position • 0.1 pN resolution, force up to 30 pN • Can measure twist, writhe, and Bustamante, stretch al. (2003) at same Nature 421, time 423-427. Keir C Neuman & Attila Nagy. (2008) Nature Methods 5, 491-505. Rice/Physics & Astronomy Ching-Hwa Kiang
Magnetic Tweezers for DNA Unzipping Diagram of DNA unzipping with magnetic tweezers. Measurement of the sequence-dependent pauses in DNA unzipping at constant forces Rice/Physics & Astronomy Danilowicz et al. PNAS 100, 1694 (2003). Ching-Hwa Kiang
Page 1 and 2: Single-Molecule Manipulation Experi
Page 3 and 4: Single-molecule manipulation experi
Page 5 and 6: Nonequilibrium Statistical Mechanic
Page 7 and 8: Stretching Single Molecules with At
Page 9: Laser Optical Tweezers Bustamante,
Page 13 and 14: Molecular Motors Finer et. al., Nat
Page 15 and 16: DNA Phase Diagram Bustamante et. al
Page 17 and 18: RNA Folding Kinetics Rice/Physics &
Page 19 and 20: Protein Folding Kinetics obtained f

Single-molecule manipulation experiments of biological molecules 1

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