Single-molecule manipulation experiments of biological molecules 1

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Applications for Single-Molecule Manipulation Force (N) X min (m) Stiffness (force const. N/m) Applications Advantages Cantilevers (SFM/AFM) 10 -12 -10 -7 10 -10 0.001-100 Protein Polysaccharides Bond strength High spatial resolution Large dynamic range Strong interactions Microneedles 10 -12 -10 -10 10 -9 10 -5 -1 Myosin motor force DNA/titin strength Flow field 10 -13 -10 -9 10 -8 - DNA dynamics RNA polymerase Magnetic field 10 -14 -10 -11 10 -8 - DNA entropic elasticity Topoisomerases activity Good operator control Soft spring constant Rapid buffer exchange Simple design Specificity to magnets Ability to induce torque Photon field 10 -13 -10 -10 10 -9 10 -10 -10 -3 Protein motors RNA unfolding Specific manipulation High force resolution Electric field 0-10 -12 - - Electrophoresis Rice/Physics & Astronomy Ching-Hwa Kiang
Nonequilibrium Statistical Mechanics of Single-Molecules Length scales and energy dissipation rates of various thermodynamic systems. The two systems in the boxes have been used to test fluctuation theorems and the Jarzynski’s equality Bustamante et. al., Physics Today 43 (2005) Rice/Physics & Astronomy Ching-Hwa Kiang
Page 1 and 2: Single-Molecule Manipulation Experi
Page 3: Single-molecule manipulation experi
Page 7 and 8: Stretching Single Molecules with At
Page 9 and 10: Laser Optical Tweezers Bustamante,
Page 11 and 12: Magnetic Tweezers for DNA Unzipping
Page 13 and 14: Molecular Motors Finer et. al., Nat
Page 15 and 16: DNA Phase Diagram Bustamante et. al
Page 17 and 18: RNA Folding Kinetics Rice/Physics &
Page 19 and 20: Protein Folding Kinetics obtained f

Single-molecule manipulation experiments of biological molecules 1

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