e-conservation the online Magazine 16, oct 2010.pdf

MICROBIAL STUDY OF EGYPTIAN MUMMIES
Table 5: Effect of some substrates on cellobiase activity of some fungal strains isolated from mummies and incubated for 5, 7
and 20 days at 28± 2°C.
Fungal strain A. niger H. viride P. corylophilum
Incubation
period/day
5 7 20 5 7 20 5 7 20
Casein 4.69 5.556 5.69 0.279 0.393 3.703 0.228 0.831 5.74
Cellulose 4.843 nd 6.389 0.293 nd 3.703 0.243 nd 1.179
Oil 4.89 6.482 6.945 0.105 0.115 0.122 0.064 0.064 0.02
Starch 7.861 5.556 5.463 0.125 0.209 0.293 1.389 2.778 4.877
Nd: not determined
c) Cellobiase enzyme
Table 5 shows that the highest cellobiase activity
(7.861 U/ml) was obtained from the culture filtrate
of A. niger after 5 days on starch substrate
and the lower level (4.690 U/ml) on casein. In
H. viride culture filtrate the highest value (3.703
U/ml) was shown on cellulose substrate and the
lowest level (0.105 U/ml) on oil substrate after
5 days incubation period. High level of cellobiase
activity (5.740 U/ml) was obtained in culture filtrate
of P. corylophilum after 20 days on casein
substrate and lower cellobiase (0.020 U/ml) was
showed on oil after 20 days. The production of
cellobiase from different fungi was reported by
several authors [25, 28, 29, 30].
Effect of some fungicides on the linear growth
of some fungi
Table 6 shows the effect of some fungicides as
agar amendment on the mycelial linear growth
(A. niger, H. viride and P. corylophilum). Data indicate
that the antifungal activity of benlate, thymol
and cedar oil against fungal growth increased
with the growth in fungicide concentration. The
linear growth of P. corylophilum was completely
inhibited at all concentrations of benlate and
thymol while the growth of A. niger and H. viride
was also completely inhibited at all concentrations
of thymol and benlate at 100 ppm. The growth of
H. viride was completely inhibited at 5% of cedar
Table 6. Effect of some fungicides on the linear fungal growth of some fungi isolated from mummies.
Fungi Benlate fungicide concentration (ppm) Mean
0 6.25 12.5 50.0 100.0
Aspergillus niger
Hormodendrum viride
Penicillium corylophilum
100.0 70.0 33.3 22.2 0.0
100.0 80.0 72.1 60.0 0.0
100.0 0.0 0.0 0.0 0.0
Thymol concentration (ppm)
0 25 50 100 200
45.1
62.4
20.0
Aspergillus niger
Hormodendrum viride
Penicillium corylophilum
100.0 75.5 63.6 42.6 0.0 56.3
100.0 0.0 0.0 0.0 0.0 20.0
100.0 0.0 0.0 0.0 0.0 20.0
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