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VITSAB® Visual Indicator Tag System AB

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Vitsab<br />

Sweden<br />

TTI-use in Monitoring Flight Catering<br />

and other foods<br />

Peter Rönnow Ph.D<br />

Managing Director Vitsab Sweden<br />

May 26, 2005 Active and inteligent packing; VTT 1


Vitsab<br />

Sweden<br />

What is a Smart Label<br />

• It is a time temperature indicator (TTI) showing<br />

the accumulated time and temperature exposure.<br />

• Smart Label technology integrates data logging<br />

and alarm functions<br />

May 26, 2005 Active and inteligent packing; VTT 2


Vitsab<br />

Sweden<br />

Vitsab ®<br />

Smart Labels<br />

May 26, 2005 Active and inteligent packing; VTT 3


Vitsab<br />

Sweden<br />

Response time of smart label at<br />

different temperatures<br />

Temp °C<br />

20<br />

15<br />

10<br />

5<br />

0<br />

-5<br />

-10<br />

-15<br />

Response time<br />

hours<br />

1<br />

2<br />

3.5<br />

7.2<br />

14<br />

33<br />

72<br />

210<br />

May 26, 2005 Active and inteligent packing; VTT 4


Vitsab<br />

Sweden<br />

Plot of response time to colour change<br />

20<br />

10<br />

Temp. °C<br />

0<br />

10<br />

0 50 100 150 200<br />

Hours<br />

Response time to colour change at different temperatures<br />

May 26, 2005 Active and inteligent packing; VTT 5


Vitsab<br />

Sweden<br />

Arrhenius equation<br />

k<br />

Ea<br />

−<br />

R ⋅T<br />

= A ⋅ e<br />

ln<br />

k<br />

=<br />

ln<br />

A<br />

−<br />

Ea<br />

R<br />

⋅<br />

1<br />

T<br />

k = rate of reaction<br />

A = constant of the equation<br />

R = universal gas constant<br />

T = absolute temperature °K<br />

Ea = Arrhenius energy of activation<br />

May 26, 2005 Active and inteligent packing; VTT 6


Vitsab<br />

Sweden<br />

Arrhenius plot of experimental data<br />

Arrhenius plot<br />

0<br />

2<br />

ln k<br />

4<br />

3.4 3.5 3.6 3.7 3.8 3.9<br />

1/T * 1000<br />

May 26, 2005 Active and inteligent packing; VTT 7


Vitsab<br />

Sweden<br />

What are Smart Labels used for<br />

• Monitoring cold chain transports<br />

• Complement to best before or use by dating<br />

May 26, 2005 Active and inteligent packing; VTT 8


Vitsab<br />

Sweden<br />

Logged temperature profile of air shipment<br />

20<br />

S<br />

F<br />

15<br />

Temperature °C<br />

10<br />

5<br />

0<br />

0 5 10 15 20<br />

Hours<br />

Fig. 4 Logged temperature profile during air shipment<br />

May 26, 2005 Active and inteligent packing; VTT 9


Vitsab<br />

Sweden<br />

Logged temperature profile of road shipment<br />

25<br />

S<br />

F<br />

20<br />

Temperature °C<br />

15<br />

10<br />

5<br />

0<br />

0 10 20 30 40<br />

Hours<br />

Fig. 5 Logged temperature profile during road shipment<br />

May 26, 2005 Active and inteligent packing; VTT 10


Vitsab<br />

Sweden<br />

Formula for Numerical Integration<br />

fA ( )<br />

n<br />

n 1<br />

= s 1<br />

e<br />

Ea<br />

RT .<br />

( n 1)<br />

RT .<br />

s<br />

1 .<br />

2 e Ea<br />

e<br />

Ea<br />

RT .<br />

n<br />

f(A) = total value of integration<br />

n = number of data points<br />

s = starting point of integration<br />

T i<br />

= temperature of each logged point<br />

May 26, 2005 Active and inteligent packing; VTT 11


Vitsab<br />

Sweden<br />

Comparison of cool chain quality between<br />

air and surface transport<br />

Transport time Corresponding transport time<br />

hours<br />

at +5 °C hours<br />

Cool chain requirement 48<br />

Air shipment 20<br />

Surface (truck) 40<br />

23<br />

26<br />

May 26, 2005 Active and inteligent packing; VTT 12


Vitsab<br />

Sweden<br />

Logged temperature profile of air shipment<br />

30<br />

S<br />

F<br />

Temperature °C<br />

20<br />

10<br />

0<br />

0 5 10 15 20 25<br />

Hours<br />

Fig. 6 Logged temperature profile of air shipment<br />

May 26, 2005 Active and inteligent packing; VTT 13


Vitsab<br />

Sweden<br />

Cool Chain quality of 2 nd air shipment<br />

Transport time corresponding transport time<br />

hours<br />

at +5 °C hours<br />

Cool chain requirement 48<br />

Air shipment 25 61<br />

May 26, 2005 Active and inteligent packing; VTT 14


Vitsab<br />

Sweden<br />

Food regulations for catered food<br />

All meals from kitchen consumed within<br />

Exposure to temperatures above 10 °C<br />

24 hours<br />

max 4 hours<br />

Bacterial growth is the general concern behind health<br />

and food regulations<br />

Low temperatures such as 2 – 8 °C<br />

give no or slow growth of bacteria<br />

Shelf life (safe to eat) below 8 °C<br />

2 – 3 days<br />

May 26, 2005 Active and inteligent packing; VTT 15


Vitsab<br />

Sweden<br />

British Airways TTI requirements<br />

for their in flight catered food<br />

• 24 hours at 7 °C<br />

• 4 hours above 10 °C<br />

May 26, 2005 Active and inteligent packing; VTT 16


Vitsab<br />

Sweden<br />

Temperature profile from data logger<br />

30<br />

Logged temperatures LGW KIN and back<br />

25<br />

20<br />

Temperature °C<br />

15<br />

10<br />

5<br />

0<br />

0 10 20 30 40 50 60 70 80 90 100<br />

Hours<br />

May 26, 2005 Active and inteligent packing; VTT 17


Vitsab<br />

Sweden<br />

Temperature profile in food trolley during return<br />

flight Kingston to LHR<br />

25<br />

Logged temperatures<br />

S<br />

F<br />

Temperature °C<br />

20<br />

15<br />

Teff<br />

For the indicated<br />

monitoring time<br />

Teff = 19.0 °C<br />

10<br />

5<br />

0 1 2 3 4 5 6 7<br />

Hours<br />

May 26, 2005 Active and inteligent packing; VTT 18


Vitsab<br />

Sweden<br />

Temperature profile of trolley with meals<br />

during flight - Tiblisi to LHR<br />

Logged temperatures<br />

20<br />

S<br />

F<br />

Temperature °C<br />

15<br />

10<br />

5<br />

Teff<br />

Homebound<br />

Reading Hours Event<br />

0 0.0 Logger placed in trolley<br />

6 0.3 Transport to aircraft<br />

38 1.9 Loading of aircraft<br />

46 2.3 Take off<br />

72 3.6 Service ended<br />

89 4.5 Logger retieved<br />

For the indicated<br />

monitoring time<br />

Teff = 7.0 °C<br />

0<br />

0 1 2 3 4<br />

Hours<br />

May 26, 2005 Active and inteligent packing; VTT 19


Vitsab<br />

Sweden<br />

Important points to assess health risk<br />

Number of bacteria that will cause harm<br />

Time to reach harmful level<br />

May 26, 2005 Active and inteligent packing; VTT 20


Vitsab<br />

Sweden<br />

Growth of bacteria at different temperatures<br />

Example of growth curves at 22, 11 and 10 °C<br />

8<br />

Log N<br />

6<br />

4<br />

4<br />

2<br />

0 5 10 15 20 25 30<br />

Hours<br />

May 26, 2005 Active and inteligent packing; VTT 21


Vitsab<br />

Sweden<br />

Type of meals evaluated for bacterial growth<br />

Chefs Salad<br />

Chicken Liver Canapé<br />

Mustard and Pommery Mayonnaise<br />

Prawn and Mango Salad<br />

Queen of Pudding<br />

Treacle tart<br />

May 26, 2005 Active and inteligent packing; VTT 22


Vitsab<br />

Sweden<br />

Predictive challenge test using<br />

Food Micromodel developed by<br />

Leatherhead Food Research Association<br />

Time (hours) to reach a maximum<br />

level of 1000 cfu/g<br />

° C<br />

Staph.<br />

aureus<br />

Listeria<br />

monocytogenes<br />

Bacillus<br />

cereus<br />

Flight Label run<br />

time (hours)<br />

22<br />

8<br />

18<br />

18<br />

3.2<br />

15<br />

22<br />

72<br />

96<br />

8.0<br />

10<br />

72<br />

156<br />

144<br />

15.8<br />

May 26, 2005 Active and inteligent packing; VTT 23


Vitsab<br />

Sweden<br />

Flight label temperature characteristics<br />

Temperature °C<br />

Response times of Vitsab smart labels for British Airways 17 hour flights<br />

25<br />

24<br />

20<br />

15<br />

10<br />

7<br />

5<br />

0<br />

0 5 10 15 20 25 30 35 40 45 50<br />

Time hours<br />

t<br />

= hours () t<br />

22<br />

21<br />

20<br />

19<br />

18<br />

17<br />

16<br />

15<br />

14<br />

13<br />

12<br />

11<br />

10<br />

9<br />

8<br />

7<br />

6<br />

5<br />

3.1<br />

3.5<br />

4.0<br />

4.6<br />

5.2<br />

6.0<br />

6.8<br />

7.8<br />

9.0<br />

10.3<br />

11.8<br />

13.6<br />

15.6<br />

18.0<br />

20.8<br />

24.0<br />

27.7<br />

32.1<br />

=<br />

May 26, 2005 Active and inteligent packing; VTT 24


Vitsab<br />

Sweden<br />

Vitsab ® Flight Label<br />

Smart Label concept for monitoring<br />

catered meals on board BA flights<br />

Developed in co-operation with British Airways<br />

May 26, 2005 Active and inteligent packing; VTT 25


Vitsab<br />

Sweden<br />

Flight Label in trolley onboard aircraft<br />

May 26, 2005 Active and inteligent packing; VTT 26


Vitsab<br />

Sweden<br />

Temperature profile in food trolley during return<br />

flight Kingston to LHR<br />

25<br />

S<br />

Logged temperatures<br />

F<br />

For the indicated<br />

monitoring time<br />

Temperature °C<br />

20<br />

15<br />

Teff<br />

Teff = 19.0 °C<br />

Flight Label uses<br />

85 % of its total<br />

response or run<br />

time<br />

10<br />

5<br />

0 1 2 3 4 5 6 7<br />

Hours<br />

May 26, 2005 Active and inteligent packing; VTT 27


Vitsab<br />

Sweden<br />

Temperature profile of trolley with meals<br />

during flight - Tiblisi to LHR<br />

20<br />

15<br />

S<br />

Logged temperatures<br />

F<br />

Homebound<br />

Reading Hours Event<br />

0 0.0 Logger placed in trolley<br />

6 0.3 Transport to aircraft<br />

38 1.9 Loading of aircraft<br />

46 2.3 Take off<br />

72 3.6 Service ended<br />

89 4.5 Logger retieved<br />

Temperature °C<br />

10<br />

5<br />

Teff<br />

For the indicated time<br />

Teff = 7.0 °C<br />

Flight Label uses 22 % of<br />

its total response or run<br />

time<br />

0<br />

0 1 2 3 4<br />

Hours<br />

May 26, 2005 Active and inteligent packing; VTT 28


Vitsab<br />

Sweden<br />

Return of yellow flight labels applied at London<br />

airports Heathrow and Gatwick during<br />

mid September – October 2003<br />

Departing airport<br />

London Heathrow<br />

London Gatwick<br />

Smart labels found<br />

yellow on board<br />

0.43 %<br />

1.19 %<br />

May 26, 2005 Active and inteligent packing; VTT 29


Vitsab<br />

Sweden<br />

Commercial benefits of flight labels<br />

1. Protection of public and cabin crew<br />

2. Protection against civil and criminal<br />

actions in case of a food poising case for<br />

the company (BA)<br />

3. In the future it will allow the airline (BA)<br />

to operate with return catering<br />

4. It will allow the airline (BA) to manage<br />

un-planed effects at airports (delays etc.)<br />

May 26, 2005 Active and inteligent packing; VTT 30


Vitsab<br />

Sweden<br />

Reduced Oxygen Packed Seafood<br />

May 26, 2005 Active and inteligent packing; VTT 31


Vitsab<br />

Sweden<br />

Time and temperatures conditions for toxin<br />

formation by Clostridium botulinum<br />

Skinner & Larkin<br />

J. Food Protection 61:9, 1154-1160,<br />

1160, 1998<br />

Toxin formation boundary<br />

25<br />

Lag time to toxin formation (Days)<br />

20<br />

15<br />

10<br />

5<br />

0<br />

40 50 60 70 80<br />

Temp. °F<br />

log(LT) =<br />

0.65 − 0.0525 ⋅ t + 2.74<br />

⋅<br />

1<br />

t<br />

May 26, 2005 Active and inteligent packing; VTT 32


Vitsab<br />

Sweden<br />

TTI characteristics of Toxin formation of<br />

Clostridium botulinum<br />

Data=<br />

°C Days<br />

4 13.3<br />

5 8.62<br />

6 6.19<br />

7 4.72<br />

8 3.74<br />

9 3.03<br />

10 2.51<br />

11 2.1<br />

12 1.77<br />

13 1.51<br />

14 1.29<br />

15 1.11<br />

ln K<br />

0<br />

1<br />

2<br />

Arrhenius plot<br />

3.5 3.55 3.6<br />

1/T * 1000<br />

Data from Skinner & Larkin J. Food Protection 61:9, 1154-1160, 1998<br />

May 26, 2005 Active and inteligent packing; VTT 33


Vitsab<br />

Sweden<br />

Application of a standard TTI formulation to<br />

Clostridium bototulinum toxin formation<br />

Fitness of M2-10 formulation to Skinner & Larkin boundry<br />

25<br />

35<br />

20<br />

Time, days<br />

15<br />

10<br />

5<br />

0<br />

35 40 45 50 55 60 65 70 75<br />

Skinner & Larkin boundry<br />

M2-10<br />

Temp. °F<br />

May 26, 2005 Active and inteligent packing; VTT 34


Vitsab<br />

Sweden<br />

Adaptation of a new Vitsab TTI formulation<br />

to Clostridium bototulinum toxin formation<br />

Fitness of L5-8 formulation to Skinner & Larkin boundry<br />

25<br />

35<br />

20<br />

Time, days<br />

15<br />

10<br />

5<br />

0<br />

35 40 45 50 55 60 65 70 75<br />

Temp. °F<br />

Skinner & Larkin boundry<br />

L5-8<br />

May 26, 2005 Active and inteligent packing; VTT 35


Vitsab<br />

Sweden<br />

Use of TTIs or Smart Labels applied to<br />

fresh and perishable food products<br />

• Smart Labels have a dynamic response to temperature changes.<br />

• Smart Labels are a powerful and cost effective tool to monitor cold<br />

transport of a variety of fresh foods including ready to eat and MAP<br />

seafood.<br />

• Smart Labels give a time and temperature response reflecting actual<br />

changes in food products<br />

May 26, 2005 Active and inteligent packing; VTT 36


Vitsab<br />

Sweden<br />

VITS<strong>AB</strong> ® Sweden <strong>AB</strong><br />

Dr. Peter Rönnow<br />

Managing Director<br />

Stenyxegatan 21<br />

S-213 76 MALMÖ<br />

Sweden<br />

Phone +46 40 215020<br />

Fax +46 40 212420<br />

E-mail: peter.ronnow@vitsab.se<br />

May 26, 2005 Active and inteligent packing; VTT 37

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