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<strong>Plant</strong> <strong>Growth</strong> <strong>in</strong> <strong>Aquaponic</strong> <strong>System</strong> <strong>through</strong> <strong>Comparison</strong> <strong>of</strong> <strong>Different</strong> <strong>Plant</strong> Media<br />

Jessica Mader<br />

Senior Honors Project<br />

Submitted <strong>in</strong> partial fulfillment <strong>of</strong> the graduation requirements<br />

<strong>of</strong> the Westover Honors Program<br />

Westover Honors Program<br />

April, 2012<br />

Dr. Thomas Shahady<br />

Dr. Nancy Cowden<br />

Dr. Priscilla Gannicott


Abstract<br />

<strong>Aquaponic</strong>s is a form <strong>of</strong> susta<strong>in</strong>able agriculture that comb<strong>in</strong>es crop and fish cultivation <strong>in</strong>to a<br />

water re-circulation system. Water conta<strong>in</strong><strong>in</strong>g fish waste is pumped to the plants, where nutrient<br />

water is absorbed and utilized for plant growth. Alternatively, plants provide filter<strong>in</strong>g <strong>of</strong> the<br />

water <strong>of</strong> excess nutrients that can be toxic to the fish. This experiment tested two food crops<br />

(lettuce and radish) grown <strong>in</strong> three different medias (soil, coconut fiber, gravel) <strong>in</strong> two separate<br />

aquaponics systems (Nutrient Film Technique (NFT) and Float<strong>in</strong>g Raft (FR)) to determ<strong>in</strong>e which<br />

media maximized plant growth <strong>in</strong> both systems. Each plant was planted and replicated <strong>in</strong> each<br />

pot (3X) and differ<strong>in</strong>g media (3X) as seed and grown for 8 weeks (NFT) and 5 weeks (FR).<br />

<strong>Growth</strong> rates were measured by record<strong>in</strong>g heights weekly and biomass (mg) at the end <strong>of</strong> the<br />

experiment. Both lettuce and radishes had the greatest growth <strong>in</strong> soil <strong>in</strong> both systems. I believe<br />

soil is the most effective because it supplies the plants with maximum water soil without<br />

becom<strong>in</strong>g overly saturated.<br />

Introduction<br />

<strong>Aquaponic</strong>s is a form <strong>of</strong> susta<strong>in</strong>able agriculture that comb<strong>in</strong>es fish and plants <strong>in</strong> a closed<br />

re-circulat<strong>in</strong>g system. <strong>Aquaponic</strong>s is considered a form <strong>of</strong> susta<strong>in</strong>able agriculture because it uses<br />

non-renewable resources and on-farm resources efficiently while still susta<strong>in</strong><strong>in</strong>g the economic<br />

viability <strong>of</strong> the farm (Susta<strong>in</strong>able Agriculture: Def<strong>in</strong>itions and Terms). Unlike traditional<br />

farm<strong>in</strong>g, <strong>in</strong> an aquaponic system there is a constant flow <strong>of</strong> water and more constant supply <strong>of</strong><br />

nutrients for the plants. The waste created by the fish provides necessary nutrients for the plants<br />

while nutrient uptake by the plants improves the water quality for the fish (Rakocy, 1989). A<br />

separate bi<strong>of</strong>ilter is not needed because the plants act as a water filter when they absorb the<br />

excess nutrients that can otherwise become toxic to the fish if too many harmful compounds<br />

build up. Fish release ammonia <strong>through</strong> their waste, and high levels <strong>of</strong> this waste product can be<br />

toxic to the fish, however these levels can be reduced <strong>through</strong> the process <strong>of</strong> nitrification.<br />

Nitrify<strong>in</strong>g bacteria convert ammonia to nitrite, then nitrate, a form <strong>of</strong> nitrogen that can be utilized<br />

by plants. Thus, when the water returns to the fish tanks, nitrogen levels are tolerable for the fish<br />

(Rakocy et al., 2006). The goal <strong>in</strong> us<strong>in</strong>g an aquaponic system is to <strong>in</strong>crease production while


m<strong>in</strong>imiz<strong>in</strong>g nutrient <strong>in</strong>puts and the consumption <strong>of</strong> water. The plants act as a filter for the water,<br />

which m<strong>in</strong>imizes total consumption <strong>of</strong> water <strong>through</strong>out the grow<strong>in</strong>g season because it is<br />

cont<strong>in</strong>ually recycled <strong>through</strong>out the system and does not need to be replaced with new, clean<br />

water.<br />

There are many different variations <strong>of</strong> aquaponic systems. The Nutrient Film Technique,<br />

or NFT, uses a cont<strong>in</strong>uous flow <strong>of</strong> a th<strong>in</strong> layer, or film <strong>of</strong> water to deliver nutrients to the plants<br />

(Figure 1). The plants are placed on a trough and a th<strong>in</strong> layer <strong>of</strong> water is pumped to the trough<br />

and a cont<strong>in</strong>uous supply <strong>of</strong> nutrients is provided for the plants (Rakocy, 1999). The media <strong>in</strong><br />

which the plants are grown absorb the water as it flows down the trough and delivers it to the<br />

plant roots (Rakocy, 1999). The water that is not absorbed by the media is returned to the fish<br />

tanks with more tolerable levels <strong>of</strong> nutrients that will not kill the fish. Some water is lost due to<br />

transpiration and evaporation at the leaf surface.


Figure 1 – NFT <strong>System</strong> Utilized at Lynchburg Grows <strong>in</strong> Lynchburg, Virg<strong>in</strong>ia. Water from the<br />

bottom trough is pumped up to the upper two levels, where a th<strong>in</strong> layer <strong>of</strong> water flows down and<br />

returns back to the bottom trough on the opposite side.<br />

Another aquaponics variation is the float<strong>in</strong>g raft technique (Figure 2). This system<br />

requires troughs with a m<strong>in</strong>imum <strong>of</strong> a foot <strong>of</strong> water. A “raft”-like structure conta<strong>in</strong><strong>in</strong>g <strong>in</strong>dividual<br />

pots with the designated media float on the water so roots grow directly <strong>in</strong>to the water. Seedl<strong>in</strong>gs<br />

can be started <strong>in</strong> pots out <strong>of</strong> the system and after they germ<strong>in</strong>ated, are placed <strong>in</strong> the raft’s net pots<br />

or started directly <strong>in</strong> the rafts <strong>in</strong> the troughs. This system provides maximum exposure <strong>of</strong> roots to<br />

the nutrient-filled water. This system is ideal for cultivation <strong>of</strong> leafy greens or other types <strong>of</strong><br />

vegetables (Rakocy et al., 2006). This system does leave roots vulnerable to damage by aquatic<br />

organisms, such as zooplankton or bacteria (Rakocy et al., 2006).<br />

Figure 2 – Float<strong>in</strong>g Raft <strong>System</strong> Utilized at Lynchburg Grows <strong>in</strong> Lynchburg, Virg<strong>in</strong>ia. The water<br />

from the bottom trough is pumped up to the upper troughs <strong>through</strong> a pipe. The water enters at<br />

one corner and dra<strong>in</strong>s out <strong>of</strong> the opposite corner.


There are many different medias <strong>in</strong> which to grow plants, and each has advantages and<br />

disadvantages. The medias used <strong>in</strong> this experiment were gravel, soil, and coconut fiber. Gravel<br />

has a large surface area for bacteria to grow and perform nitrification. Also, the composition the<br />

gravel itself can provide some nutrients for plants as the water weathers the rock, for example<br />

calcium can be released as the gravel reacts with acid produced dur<strong>in</strong>g nitrification (Rakocy et<br />

al., 2006). A disadvantage is that the gravel reta<strong>in</strong>s very little water. Soil has the ability to supply<br />

the plants with enough water without becom<strong>in</strong>g overly saturated. Because soil is composed <strong>of</strong><br />

smaller gra<strong>in</strong> sizes compared to gravel, it is able to reta<strong>in</strong> some water while the excess dra<strong>in</strong>s out.<br />

There are also pore spaces that provide the plant roots with air (McCauley et al., 2005). Soil<br />

naturally conta<strong>in</strong>s nutrients, but does not always conta<strong>in</strong> enough for healthy plant growth and it<br />

can be depleted <strong>of</strong> nutrients as the plant grows. Coconut fiber is made from the husk <strong>of</strong> a<br />

coconut. It is highly absorbent material but it does not conta<strong>in</strong> abundant nutrients. The different<br />

medias provide surface area for bacteria to grow and perform nitrification, and capture solids<br />

before they reach the plant roots (Rakocy et al., 2006). A good medium for plant growth creates<br />

a nutrient pool around the plant roots and provides adequate air space for respiration (Sikawa,<br />

2010).<br />

There are a total <strong>of</strong> 18 essential nutrients for maximum plant growth. The three<br />

macronutrients are carbon, oxygen, and hydrogen, which are supplied by the water and carbon<br />

dioxide gas. Other macronutrients provided by the nutrient filled water <strong>in</strong>clude nitrogen,<br />

potassium, calcium, magnesium, phosphorus, and sulfur. The rema<strong>in</strong><strong>in</strong>g nutrients are<br />

micronutrients. A balance <strong>of</strong> all these nutrients provides for optimal plant growth (Rakocy et al.,<br />

2006).


ADD PARAGRAPH: k<strong>in</strong>d <strong>of</strong> plants that are grown <strong>in</strong> hydroponics, <strong>in</strong>dicate that the same<br />

plants will work <strong>in</strong> aquaponics<br />

Radishes are edible members <strong>of</strong> the Brassicaceae (Figure 3). Radishes require even and<br />

moderate to heavy water<strong>in</strong>g and prefer full sun, but can tolerate partial shade (Denckia, 2003).<br />

They require low levels <strong>of</strong> nitrogen, phosphorus, and potassium. They do best <strong>in</strong> cool, moist soil<br />

and a pH between 6.0 and 7.0. Radishes germ<strong>in</strong>ate between 4 to 12 days.<br />

Figure 3 – Radish plant grown <strong>in</strong> soil <strong>in</strong> the NFT system at 7 weeks.<br />

Lettuce belongs to the Asteraceae (Figure 4). It does not require a lot <strong>of</strong> water, but it does<br />

need moderate water consistently and, like radishes, prefers full sun, but can tolerate partial<br />

shade (Denckia, 2003). Lettuce plants require high levels <strong>of</strong> nitrogen, phosphorus, and<br />

potassium. The lettuce plant has a relatively shallow, compact root system that does not absorb<br />

nutrients and moisture from the soil very efficiently. The optimum soil pH is between 6.5 and<br />

7.0. The plant typically germ<strong>in</strong>ates with<strong>in</strong> 7 to 14 days.


Figure 4 – Lettuce plant grown <strong>in</strong> the soil <strong>in</strong> the NFT system at 7 weeks.<br />

In this experiment, lettuce and radishes were grown <strong>in</strong> three different media to determ<strong>in</strong>e<br />

which media=um resulted <strong>in</strong> the greatest plant performance. Each plant type was grown <strong>in</strong> soil,<br />

gravel, and coconut fiber as different treatments. Also, two different aquaponic systems were<br />

used; the NFT and the float<strong>in</strong>g raft system. The goal for this experiment was to determ<strong>in</strong>e which<br />

crop and which medium yielded the greatest growth performance.<br />

Methods<br />

Study Site<br />

This experiment was conducted at Lynchburg Grows, located <strong>in</strong> Lynchburg, VA,<br />

(3724’13’’N 7910’12’’W), is an urban farm that practices susta<strong>in</strong>able agriculture techniques.<br />

There are a total <strong>of</strong> n<strong>in</strong>e greenhouses with one dedicated to aquaponics. The construction <strong>of</strong> the<br />

Nutrient Film Technique system was completed <strong>in</strong> September <strong>of</strong> 2011 and the construction <strong>of</strong> the<br />

float<strong>in</strong>g raft system was completed <strong>in</strong> October <strong>of</strong> 2011.


Experimental Design<br />

Many different types <strong>of</strong> plants can be grown <strong>in</strong> an aquaponic system. The crops chosen<br />

for this experiment needed to be suited for grow<strong>in</strong>g <strong>in</strong> the late summer/early fall. They also<br />

needed to grow quickly so that growth data could be collected. Lettuce and radishes were two<br />

crops that were suitable for this experiment. Lettuce is a suitable crop because it grows <strong>in</strong> short<br />

periods <strong>of</strong> time and produce few pest problems (Rakocy et al., 2006). Radishes germ<strong>in</strong>ate<br />

quickly and require low levels <strong>of</strong> nutrients. Lynchburg Grows provided the seeds for each plant.<br />

Lynchburg Grows could easily cont<strong>in</strong>ue grow<strong>in</strong>g this crops <strong>in</strong> the aquaponic system and sell<br />

them for pr<strong>of</strong>it if we determ<strong>in</strong>e an aquaponic system that worked best.<br />

Lynchburg Grows provided all the seeds, pots, and media for the project. In the NFT,<br />

radish and lettuce were planted from seeds. For each crop, three seeds <strong>of</strong> one type <strong>of</strong> plant were<br />

planted <strong>in</strong> one pot and seven pots were completed for each <strong>of</strong> the three media, for a total <strong>of</strong> 21<br />

seeds per medium per plant type. A th<strong>in</strong> layer <strong>of</strong> soil was placed only on the top <strong>of</strong> the gravel to<br />

act as a bed to hold the seed before it germ<strong>in</strong>ated. The pots were labeled and randomly arranged<br />

by a fellow student and I distribut<strong>in</strong>g them along the top trough. They were planted on<br />

September 12, 2011. (Variety <strong>of</strong> each plant, orig<strong>in</strong>al source <strong>of</strong> seeds- where did LG get them)<br />

In the float<strong>in</strong>g raft system, eight holes were cut <strong>in</strong> a styr<strong>of</strong>oam board where small pots<br />

were placed and the pots extended 2 <strong>in</strong>ches <strong>in</strong>to the water. This was done to four styr<strong>of</strong>oam<br />

boards. Radishes and lettuce seeds were planted <strong>in</strong> the soil, coconut fiber and gravel. There were<br />

a total pots, each with 2 seeds, for each treatment. The treatments were randomly arranged by<br />

number<strong>in</strong>g the empty pots, mak<strong>in</strong>g a list <strong>of</strong> all the treatments, draw<strong>in</strong>g numbers out <strong>of</strong> a pot, and<br />

go<strong>in</strong>g down the list assign<strong>in</strong>g pot numbers to the treatments. All the treatments were listed, a


number that corresponded to a pot on the raft was drawn from a pot, and that number was<br />

assigned to the treatment next on the list. Two pots were left empty <strong>in</strong> order to have an equal<br />

number <strong>of</strong> pots per treatment. All plants were planted on October 3, 2011. (Pot size- <strong>in</strong>ternal<br />

volume- how much grow<strong>in</strong>g media was available to the plants)<br />

Two hundred channel catfish were added to the NFT and the same number to the float<strong>in</strong>g<br />

raft system on October 26 (Figure 5). This date for stock<strong>in</strong>g was after my plant<strong>in</strong>g date due to<br />

availability <strong>of</strong> fish. Channel catfish were obta<strong>in</strong>ed from a hatchery <strong>in</strong> eastern Virg<strong>in</strong>ia, were<br />

pellet tra<strong>in</strong>ed and a good choice for this type <strong>of</strong> system (Shahady personal commentation). While<br />

the fish are an important component <strong>of</strong> an aquaponic system, this project was designed to study<br />

plants thus any discussion <strong>of</strong> fish <strong>in</strong> this system is a separate study. (where were the fish<br />

obta<strong>in</strong>ed from- what hatchery, age/size)<br />

(picture to come)<br />

Figure 5 – Channel Catfish stocked <strong>in</strong>to the <strong>Aquaponic</strong> units.<br />

Type <strong>of</strong> system<br />

Construction <strong>of</strong> the system was based on similar units <strong>in</strong> use at Grow<strong>in</strong>g Power Inc.<br />

(where is it located) (Will Allen personal communication, 2010). The types <strong>of</strong> systems used<br />

were the nutrient film technique (NFT) and float<strong>in</strong>g raft. In the NFT, a th<strong>in</strong> layer <strong>of</strong> constantly<br />

flow<strong>in</strong>g water provides a cont<strong>in</strong>uous supply <strong>of</strong> water, nutrients, and oxygen. In the float<strong>in</strong>g raft<br />

system, plants roots grow directly <strong>in</strong>to a conta<strong>in</strong>er <strong>of</strong> water. The rafts provide optimum root<br />

exposure to the nutrient water (Rakocy, 1988). The styr<strong>of</strong>oam boards also shield the water from<br />

direct sun light to help ma<strong>in</strong>ta<strong>in</strong> lower water temperatures, which is beneficial for plant growth<br />

(Rakocy, 1988). (re-word)


Lettuce and radishes were chosen because they are rapidly grow<strong>in</strong>g plants that can do<br />

well <strong>in</strong> the reduced day length and cooler temperatures experienced <strong>in</strong> the fall <strong>in</strong> the Virg<strong>in</strong>ia<br />

region. These plant types were readily available at Lynchburg Grows. The chosen media for this<br />

experiment were soil, gravel, and coconut fiber, which were also provided by Lynchburg Grows.<br />

All the media types were found on the Lynchburg Grows property, mak<strong>in</strong>g it convenient to use<br />

for the experiment. (Details about the media)<br />

Once per week, the height <strong>of</strong> each lettuce and radish plant was measured <strong>in</strong> centimeters.<br />

The heights were used to measure growth for each plant over time. Temperature, pH,<br />

conductivity, and dissolved oxygen read<strong>in</strong>gs were taken at the at the <strong>in</strong>flow and outflow end <strong>of</strong><br />

the system. These parameters were used to access the water quality and make sure it was with<strong>in</strong><br />

adequate range for the plants and the fish. Water samples were also taken at the <strong>in</strong>flow and<br />

outflow end every week to measure nutrient levels available for the plants at entry and utilization<br />

by the plants <strong>in</strong> the experiment.<br />

The nutrients analyzed are phosphorus, nitrate and ammonia us<strong>in</strong>g standard protocols<br />

developed for the Systea Scientific discrete auto-analyzer (Easy Chem Methology, 2009) (Put <strong>in</strong><br />

lit. cited). These nutrients were measured to ensure the adequate levels were available for<br />

optimal plant growth.<br />

All the plants from both systems were harvested on November 15, 2011. The NFT<br />

heights were recorded for a total <strong>of</strong> eight weeks, while the float<strong>in</strong>g raft plants were recorded for<br />

five weeks. The plants <strong>in</strong> the float<strong>in</strong>g raft had less weeks because the construction <strong>of</strong> the system<br />

was not completed until mid October. All the plants had to harvested at the same time, regardless


<strong>of</strong> this time difference, because it was the gett<strong>in</strong>g near the end <strong>of</strong> the grow<strong>in</strong>g season and the<br />

temperatures were beg<strong>in</strong>n<strong>in</strong>g to get too cold. This experiment was only concerned with growth<br />

over time <strong>in</strong> different media. The radishes and the lettuce plants were carefully removed from the<br />

pots. Then the plant was cut were the roots met the stem. The foliage above the soil and the roots<br />

below the soil were weighed separately to compare how much growth was dedicated to the roots<br />

compared to the leaves.<br />

Data Analysis<br />

All the measurements for each plant <strong>in</strong> each media were averaged together were plotted<br />

on a l<strong>in</strong>e graph to show growth <strong>of</strong> the plants over the course <strong>of</strong> the experiment. A s<strong>in</strong>gle factor<br />

ANOVA compared weights (total, above ground, below ground) <strong>of</strong> the plants grown <strong>in</strong> the three<br />

media. <strong>Comparison</strong>s were made for each crop across the three different media with<strong>in</strong> each<br />

<strong>in</strong>dividual system. P-values were used to test significant difference <strong>in</strong> the mean plants weights<br />

and, therefore, the overall growth among the three media.<br />

Results<br />

Radish <strong>Growth</strong> Rate <strong>in</strong> the Float<strong>in</strong>g Raft<br />

The heights if the radish heights for each media <strong>in</strong> the float<strong>in</strong>g raft system were plotted<br />

for each week (Figure 6). The graph shows a similar trend <strong>in</strong> growth rates <strong>of</strong> the radishes grown<br />

<strong>in</strong> the gravel and soil. Radishes grown <strong>in</strong> fiber showed the least overall growth. The radish<br />

plants grown <strong>in</strong> soil had the largest average end height (11.77cm), with gravel close beh<strong>in</strong>d<br />

(11.19 cm), and fiber last <strong>in</strong> end height (3.075). (ADD STAND DEV)


height (cm)<br />

14<br />

12<br />

10<br />

8<br />

6<br />

4<br />

2<br />

Grav<br />

el<br />

Fiber<br />

0<br />

1 2 3 4 5<br />

time (weeks)<br />

Figure 6- <strong>Growth</strong> rates <strong>of</strong> radishes <strong>in</strong> float<strong>in</strong>g raft system over the experimental time period <strong>in</strong><br />

each medium type. (fix fiber, gray scale-add color or different symbols, add po<strong>in</strong>ts at each week,<br />

error bars)<br />

Radish <strong>Growth</strong> Rate <strong>in</strong> the NFT<br />

The average radish heights <strong>in</strong> the NFT were plotted for each week for a total <strong>of</strong> 8 weeks<br />

(Figure 7). In the NFT, soil showed the greatest growth. There was a cont<strong>in</strong>ual growth for the<br />

first 7 weeks and then a drop the f<strong>in</strong>al week. Radish growth <strong>in</strong> coconut fiber and gravel was not<br />

as great as <strong>in</strong> soil. The radishes <strong>in</strong> the fiber had a negative growth and radishes <strong>in</strong> the gravel had<br />

a spike <strong>in</strong> growth <strong>in</strong> the 3 rd week, but then a drop for the rema<strong>in</strong>der <strong>of</strong> the experimental time.<br />

(Did the plants shr<strong>in</strong>k )


height (cm)<br />

height (cm)<br />

25<br />

20<br />

15<br />

10<br />

5<br />

0<br />

1 2 3 4 5 6 7 8<br />

time (weeks)<br />

Gravel<br />

Fiber<br />

Soil<br />

Figure 7- Radishes grown <strong>in</strong> NFT system over the experimental time period <strong>in</strong> each medium<br />

type.<br />

Lettuce <strong>Growth</strong> Rate <strong>in</strong> the Float<strong>in</strong>g Raft<br />

Po<strong>in</strong>ts were plotted on a l<strong>in</strong>e graph for the average height <strong>of</strong> lettuce plants <strong>in</strong> each<br />

treatment <strong>in</strong> the float<strong>in</strong>g raft for each week (Figure 8). Each media followed a similar growth<br />

trend, however, soil had the greatest overall growth, followed by gravel, and then fiber. Lettuces<br />

averaged 7.34 cm <strong>in</strong> height (STAND DEV) <strong>in</strong> the soil, 6.52 cm <strong>in</strong> height (STAND DEV) <strong>in</strong> the<br />

gravel, and 5.7 cm <strong>in</strong> height (STAND DEV) <strong>in</strong> the fiber at the end <strong>of</strong> the experiment.<br />

8<br />

7<br />

6<br />

5<br />

4<br />

3<br />

2<br />

1<br />

0<br />

1 2 3 4 5<br />

time (weeks)<br />

Gravel<br />

Fiber<br />

Soil


height (cm)<br />

Figure 8– Lettuce grown <strong>in</strong> Float<strong>in</strong>g Raft system over the experimental time period <strong>in</strong> each<br />

media type.<br />

Lettuce <strong>Growth</strong> Rate <strong>in</strong> the NFT<br />

The average heights for each week were plotted on a l<strong>in</strong>e graph to depict growth <strong>in</strong> each<br />

medium (Figure 9). Soil was the most effective medium for lettuce <strong>in</strong> the NFT method as<br />

assessed by average height over time. Lettuce grown <strong>in</strong> the soil showed much growth <strong>in</strong> the 8<br />

weeks, with an average f<strong>in</strong>ished height <strong>of</strong> 18.12 cm. Lettuce grown <strong>in</strong> soil showed only a small<br />

amount <strong>of</strong> growth over the 8 weeks. <strong>Plant</strong>s grown <strong>in</strong> gravel showed only a small amount <strong>of</strong><br />

growth with only 1 cm <strong>of</strong> growth over the experiment. (STAND DEV)<br />

20<br />

18<br />

16<br />

14<br />

12<br />

10<br />

8<br />

6<br />

4<br />

2<br />

0<br />

1 2 3 4 5 6 7 8<br />

time (weeks)<br />

Gravel<br />

Fiber<br />

Soil<br />

Figure 9- Lettuce grown <strong>in</strong> NFT system over the experimental time period <strong>in</strong> each media type.<br />

Radish Weights <strong>in</strong> the Float<strong>in</strong>g Raft <strong>System</strong><br />

To obta<strong>in</strong> the weights <strong>of</strong> the plants, a SCALE Was used. The majority <strong>of</strong> weight for the<br />

radish plants came from the leaves/shoots (Table 1). Radishes grown <strong>in</strong> soils showed the greatest<br />

average total weight, above ground weight, and below ground weight.


Table 1- Average biomass for radishes <strong>in</strong> Float<strong>in</strong>g Raft (STAND DEV)<br />

MEDIA TOTAL (g) SHOOTS (g) ROOTS (g)<br />

GRAVEL 5.86 4.41 1.44<br />

FIBER 0.60 0.38 0.22<br />

SOIL 8.74 7.10 1.64<br />

Radishes Weights <strong>in</strong> the NFT<br />

There were no plants to weigh at the end <strong>of</strong> the experiment for radish plants grown <strong>in</strong><br />

gravel (Table 2). Almost all <strong>of</strong> the total weight for the radish plants grown <strong>in</strong> soil comes from the<br />

shoot <strong>of</strong> the plant and the root represents a very small proportion <strong>of</strong> the total plant. The overall<br />

biomass <strong>of</strong> radishes grown <strong>in</strong> fiber is much less than those grown <strong>in</strong> soil, but still most <strong>of</strong> the<br />

weight comes from the shoot <strong>of</strong> the radish plant.<br />

Table 2- Average biomass for radishes <strong>in</strong> the NFT<br />

MEDIA TOTAL (g) SHOOTS (g) ROOTS (g)<br />

GRAVEL 0 0 0<br />

FIBER 0.09 0.07 0.01<br />

SOIL 17.81 17.68 0.13<br />

For total weights <strong>of</strong> radishes <strong>in</strong> the float<strong>in</strong>g raft system there was a strong significant<br />

difference <strong>in</strong> plants grown <strong>in</strong> soil compared to plants grown <strong>in</strong> fiber (Table 3). There was also a<br />

strong significant difference when plants grown <strong>in</strong> fiber were compared to plants grown <strong>in</strong>


gravel. When the plants grown <strong>in</strong> gravel were compared to plants grown <strong>in</strong> soil, there was no<br />

significant difference. For the shoot biomass, there was aga<strong>in</strong> a strong significance <strong>in</strong> plants<br />

grown <strong>in</strong> soil compared to plants grown <strong>in</strong> fiber and plants grown <strong>in</strong> fiber compared to those<br />

grown <strong>in</strong> gravel. There was no significant difference <strong>in</strong> plants grown <strong>in</strong> gravel and plants grown<br />

<strong>in</strong> soil.<br />

The results <strong>of</strong> the comparisons <strong>of</strong> root biomass between the three media showed there<br />

was only a statistical significance between the plants grown <strong>in</strong> the fiber and the plants grown <strong>in</strong><br />

the gravel.<br />

Table 3- <strong>Comparison</strong> show<strong>in</strong>g P-values for radish plants <strong>in</strong> different media grown <strong>in</strong> the Float<strong>in</strong>g<br />

Raft and NFT.<br />

Float<strong>in</strong>g<br />

Raft NFT<br />

Total Biomass Gravel to Soil 0.15 0.07<br />

Soil and Coconut Fiber


along with plants grown <strong>in</strong> gravel and plants grown <strong>in</strong> soil. Aga<strong>in</strong> there was no statistical<br />

difference between radishes grown <strong>in</strong> fiber and radishes grown <strong>in</strong> gravel.<br />

Lettuce Weights <strong>in</strong> the Float<strong>in</strong>g Raft <strong>System</strong><br />

For all three treatments, the majority <strong>of</strong> the weight <strong>of</strong> the lettuce came from the shoots <strong>of</strong><br />

the plant (Table 4). The greatest total weight was seen <strong>in</strong> lettuce plants grown <strong>in</strong> soil, with<br />

gravel close beh<strong>in</strong>d. Gravel as a media did not result <strong>in</strong> great overall growth for lettuce.<br />

Table 4- Average weights for lettuce <strong>in</strong> the Float<strong>in</strong>g Raft system<br />

MEDIA TOTAL (g) SHOOTS (g) ROOTS (g)<br />

GRAVEL 1.52 0.92 0.60<br />

FIBER 0.84 0.59 0.24<br />

SOIL 2.07 1.30 0.77<br />

Lettuce Weights <strong>in</strong> the NFT<br />

For all three treatments, a majority <strong>of</strong> the weight <strong>of</strong> the lettuce came from the shoots <strong>of</strong><br />

the plant (Table 5). Soil noticeably resulted <strong>in</strong> the greatest overall growth <strong>in</strong> both leaves and<br />

roots, and thus total weight (14.38 g).<br />

Table 5- Average weights for lettuce <strong>in</strong> the NFT<br />

MEDIA TOTAL (g) SHOOTS (g) ROOTS (g)<br />

GRAVEL 1.36 1.04 0.32<br />

FIBER 0.07 0.04 0.03<br />

SOIL 14.38 10.65 3.73


There was a significant difference between the total weights <strong>of</strong> lettuce grown <strong>in</strong> gravel<br />

and lettuce grown <strong>in</strong> soil (Table 6). There was not a statistical significance <strong>in</strong> the comparisons <strong>of</strong><br />

the total weights <strong>of</strong> plants grown <strong>in</strong> soil and plants grown <strong>in</strong> fiber or plants grown <strong>in</strong> fiber and<br />

plants grown <strong>in</strong> gravel. None <strong>of</strong> the comparisons <strong>of</strong> leaf or root weights were statistically<br />

significant.<br />

Table 6- P-values for lettuce plants grown <strong>in</strong> the Float<strong>in</strong>g Raft and NFT<br />

Float<strong>in</strong>g<br />

Raft<br />

NFT<br />

Total Weight Gravel to Soil 0.01 >0.001<br />

Soil to Coconut Fiber 0.79 >0.001<br />

Coconut Fiber to Gravel 0.15 0.04<br />

Shoot Weight Gravel to Soil 0.36 >0.001<br />

Soil to Coconut Fiber 0.17 >0.001<br />

Coconut Fiber to Gravel 0.28 0.039<br />

Root Weight Gravel to Soil 0.42 >0.001<br />

Soil to Coconut Fiber 0.05 >0.001<br />

Coconut Fiber to Gravel 0.08 0.07<br />

For lettuce grown <strong>in</strong> the NFT, there was large significance <strong>in</strong> the total weights when<br />

plants grown <strong>in</strong> gravel were compared to plants grown <strong>in</strong> soil (Table 6). There was a similar<br />

result for the comparison between plants grown <strong>in</strong> soil and plants grown <strong>in</strong> coconut fiber. There<br />

was a smaller significance between plants grown <strong>in</strong> coconut fiber and plants grown <strong>in</strong> gravel. For<br />

the shoot weights, there was once aga<strong>in</strong> the large significance between lettuce grown <strong>in</strong> gravel<br />

and lettuce grown <strong>in</strong> soil. There were similar results for the comparison between lettuce grown <strong>in</strong><br />

soil and lettuce grown <strong>in</strong> coconut fiber. Aga<strong>in</strong>, there was a significant difference between plants<br />

grown <strong>in</strong> coconut fiber and plants grown <strong>in</strong> gravel. There was statistical significance <strong>in</strong> the root<br />

weights between lettuce grown <strong>in</strong> gravel and lettuce grown <strong>in</strong> soil and between lettuce grown <strong>in</strong>


soil and lettuce grown <strong>in</strong> coconut fiber. No significant difference was found between plants<br />

grown <strong>in</strong> coconut fiber and plants grown <strong>in</strong> gravel for any <strong>of</strong> the weights measured.<br />

Nutrient Analysis<br />

The nutrient analysis was not preformed on a consistent basis. However, the total<br />

phosphorus levels decreased as the water flowed <strong>through</strong> the plant pots (Table 7). These water<br />

samples were taken before the catfish were placed <strong>in</strong>to the system. The nitrate and ammonia<br />

levels do not show the same consistence with regards to the <strong>in</strong>flow and outflow. (NEED HELP<br />

WITH NUTRIENTS)<br />

Table 7- Nutrient concentration for the <strong>in</strong>flow and outflow <strong>of</strong> the NFT and Float<strong>in</strong>g Raft<br />

Total P<br />

NFT<br />

In<br />

Out<br />

9/23/11 0.051 0.039<br />

9/27/11 0.067 0.06<br />

10/3/11 0.09 0.073<br />

Nitrate<br />

NFT<br />

FR<br />

In Out In Out<br />

11/4/11 0.007 0.01 0.061 0.063<br />

11/11/11 0.127 0.068 0.0171 0.052<br />

Ammonia<br />

NFT<br />

FR<br />

In Out In Out<br />

11/4/11 0.523 -0.047 0.702 0.713<br />

11/11/11 0.024 0.011 0.515 0.534<br />

Discussion<br />

The medium <strong>in</strong> which a plant is cultivated plays a major role <strong>in</strong> it overall growth. A good<br />

substrate <strong>in</strong> an aquaponic system ma<strong>in</strong>ta<strong>in</strong>s a nutrient reservoir <strong>in</strong> the root zone and provides<br />

adequate air pore space for gas exchange (Sikawa, 2010). At the end <strong>of</strong> the experiment, both


lettuce and radish plants grown <strong>in</strong> soil showed the greatest growth <strong>in</strong> the NFT (Figures 7 and 9).<br />

Lettuce performed well <strong>in</strong> the NFT because it requires consistent water<strong>in</strong>g (Denckia, 2003).<br />

Radishes need larger amounts <strong>of</strong> water than lettuce, which is still provided by the even and<br />

constant flow <strong>of</strong> water <strong>in</strong> the NFT <strong>in</strong> soil. However, the water had to make contact with the seed.<br />

The seed was planted <strong>in</strong> a tall pot and <strong>in</strong> order for the water to reach the seed. The media <strong>in</strong> the<br />

pot needed to absorb the water so the water could reach the seed and allow for germ<strong>in</strong>ation. The<br />

soil was able to absorb enough water that it reached the seed. Soil has the ability to supply the<br />

plants with enough water without becom<strong>in</strong>g overly saturated. It does not hold on to water to<br />

tightly where there is no air <strong>in</strong> the soil. Some <strong>of</strong> the spaces are filled with air, so there is an<br />

aerobic environment, which is best for plant growth (McCauley et al., 2005).<br />

Gravel did not perform well because it was unable to absorb the water need for the seed<br />

to germ<strong>in</strong>ate. The gravel was too dry for the plant, therefore there was little overall growth for<br />

both radish and lettuce plants <strong>in</strong> the NFT (Figures 7 and 9). The coconut fiber is very absorbent<br />

<strong>of</strong> water, but this can cause over saturation reduc<strong>in</strong>g plant growth. In the NFT, both lettuce and<br />

radish plants did show much growth dur<strong>in</strong>g the experiment. The coconut fiber was able to absorb<br />

the water and br<strong>in</strong>g it to the seed at the top <strong>of</strong> the pot, but became too wet <strong>of</strong> an environment.<br />

When the media becomes overly saturated, it causes poor aeration and anaerobic conditions for<br />

the roots and reduces overall growth.<br />

Soil once aga<strong>in</strong> preformed the best for both the lettuce and radish plants and resulted <strong>in</strong><br />

the greatest growth between the three medias (Figures 6 and 8). In the FR system, the roots grow<br />

directly <strong>in</strong>to the water so there is a constant supply <strong>of</strong> water and nutrients. Gravel plants <strong>in</strong> the<br />

float<strong>in</strong>g raft grew greater compared to <strong>in</strong> the NFT, maybe because the roots extended directly


<strong>in</strong>to the water, provid<strong>in</strong>g the plant with a water supply without becom<strong>in</strong>g overly saturated. The<br />

part <strong>of</strong> the pot that was above the water l<strong>in</strong>e was able to dra<strong>in</strong> allow<strong>in</strong>g air to also reach the roots.<br />

Fiber still did not perform well for the radishes because it was still over saturated for the plants<br />

(Figure 6). The lettuce plants <strong>in</strong> the fiber showed better results, but still had the least growth<br />

(Figure 8). The coconut fiber aga<strong>in</strong> became over saturated and it became anaerobic.<br />

Soil is a viable media option to use <strong>in</strong> an aquaponic system because it is abundant and easily<br />

purchased or even made by compose. Soil naturally conta<strong>in</strong>s nutrients that can be utilized by the<br />

plants.<br />

<strong>Plant</strong>s grown <strong>in</strong> soil <strong>in</strong> both the NFT and float<strong>in</strong>g raft showed considerable growth, but<br />

when compar<strong>in</strong>g both systems at the 5 week mark, the NFT had greater average growth up to<br />

that po<strong>in</strong>t for both the lettuce and radishes. The height at 5 weeks for lettuce <strong>in</strong> NFT was 15.82<br />

cm while it was only 7.34 cm <strong>in</strong> the float<strong>in</strong>g raft. Radishes had an average height <strong>of</strong> 17.37 cm <strong>in</strong><br />

the NFT, and 11.77 cm <strong>in</strong> the float<strong>in</strong>g raft. The NFT is the more a practical system to use to<br />

grow the crops. There are already two structures at Lynchburg Grows that have large areas to<br />

grow abundant amount <strong>of</strong> plants. If all the space is utilized, a large quality <strong>of</strong> plants can be<br />

grown and eventually sold for pr<strong>of</strong>it.<br />

The catfish were <strong>in</strong>troduced to the system 4 weeks after the first set <strong>of</strong> plants were<br />

planted. This means the fish provided no additional nutrients for the first month for the NFT, and<br />

first week for the float<strong>in</strong>g raft system. This could be an explanation for why plants grown <strong>in</strong> soil<br />

performed the best. The soil already conta<strong>in</strong>ed nutrients, so the plants used those nutrients for<br />

growth. The total phosphorus levels were lower <strong>in</strong> the outflow because the media absorbed some


for plant use (Table 7). The data was not completed consistently enough to determ<strong>in</strong>e the effects<br />

<strong>of</strong> the addition <strong>of</strong> the fish and just how much nutrients they added to the system.<br />

The simplest approach to design an aquaponic system is to duplicate a standard system or<br />

scale a standard system down or up. The UVI system has been well documented and is be<strong>in</strong>g<br />

studied or used commercially <strong>in</strong> several locations, design for available space (Rakocy, 2006).<br />

Several different plants can be grown <strong>in</strong> this type <strong>of</strong> agriculture method. Some plants<br />

require more nutrients and require a larger density <strong>of</strong> fish to supply those nutrients. Lettuce,<br />

herbs, and specialty greens (sp<strong>in</strong>ach, chives, basil, and watercress) have low to medium<br />

nutritional requirements and are well adapted to aquaponic systems. <strong>Plant</strong>s yield<strong>in</strong>g fruit<br />

(tomatoes, bell peppers, and cucumbers) have a higher nutritional demand and perform better <strong>in</strong><br />

a heavily stocked, well established aquaponic system (Diver, 2006).<br />

There are some advantages to grow<strong>in</strong>g crops <strong>in</strong> an aquaponic system over grow<strong>in</strong>g <strong>in</strong> the<br />

ground. Large reductions <strong>in</strong> chemical and water consumption have been achieved <strong>through</strong> the<br />

use <strong>of</strong> an aquaponic system (Rakocy, 1989). The plants act as a bio-filter, so there is no need for<br />

an separate bio-filtration system. Plus, this bio-filter produces <strong>in</strong>come for the <strong>in</strong>stitute. There is<br />

no need for addition chemicals and fertilizers because the fish provided the nutrients for the<br />

plants. The fish waste would normally be discharged and would contribute to pollution. The<br />

removal <strong>of</strong> nutrients by the plants prolongs the water use and m<strong>in</strong>imizes total water<br />

consumption. As an <strong>in</strong>tegrated system, it <strong>in</strong>creases pr<strong>of</strong>it due to free nutrients, lower water<br />

requirements, elim<strong>in</strong>ation <strong>of</strong> a separate bio-filter, less water quality monitor<strong>in</strong>g, and crops and<br />

fish can be produced <strong>in</strong> the same space and time (Rakocy et al., 2004).


References<br />

Denckia, T.L.K. (2003). The Gardener’s A-Z Guide to Grow<strong>in</strong>g Organic Food. North Adams,<br />

MA: Storey Publish<strong>in</strong>g.<br />

Diver, S. (2006). <strong>Aquaponic</strong>s—Integration <strong>of</strong> Hydroponics with Aquaculture. ATTRA, 1-28.<br />

McCauley, A., Jones, C., & Jacobsen, J. (2005). Basic Soil Properties. Montana State University<br />

Extension Service, 1-12.<br />

Rakocy, J.E. (1989). Hydroponic Lettuce Production <strong>in</strong> a Recirculat<strong>in</strong>g Fish Culture <strong>System</strong>.<br />

Island Perspectives, 3, 5-10.<br />

Rakocy, J.E. (1999). The Status <strong>of</strong> <strong>Aquaponic</strong>s, Part 1. Aquaculture Magaz<strong>in</strong>e, 83-88.<br />

Rakocy, J. E., Bailey, D.S., Shultz, R.C., & Thoman, E.S. (2004). Update on tilapia and<br />

vegetable production <strong>in</strong> the UVI aquaponic system. New Dimensions on Farmed Tilapia:<br />

Proceed<strong>in</strong>gs <strong>of</strong> the Sixth International Symposium
 on Tilapia <strong>in</strong> Aquaculture, Manila,<br />

Philipp<strong>in</strong>es. 676–690.<br />

Rakocy, J.E., Masser, M.P., & Losordo, T.M. (2006). Recirculat<strong>in</strong>g Aquaculture Tank<br />

Production <strong>System</strong>s: <strong>Aquaponic</strong>s- Integrat<strong>in</strong>g Fish and <strong>Plant</strong> Culture. Southern Regional<br />

Aquaculture Center, 454, 1-16.<br />

Sikawa, D.C., & Yakupitiyage, A. (2010). The hydroponic production <strong>of</strong> lettuce (Lactuca sativa<br />

L) by us<strong>in</strong>g hybrid catfish (Clarias macrocephalus × C. gariep<strong>in</strong>us) pond water: Potentials<br />

and constra<strong>in</strong>ts. Agricultural Water Management, 97(9), 1317-1325.<br />

Susta<strong>in</strong>able Agriculture: Def<strong>in</strong>itions and Terms. (2007). USDA National Agricultural Library.<br />

Retrieved from http://www.nal.usda.gov/afsic/pubs/terms/srb9902.shtml

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