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DMEM, High Glucose

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Medium Series<br />

Applications<br />

• For cultivation of most cell types<br />

• Usage is recommended with supplementation of 10% FBS<br />

Features<br />

• <strong>DMEM</strong> is a modification of the Eagle Medium with 4.5 g/l glucose<br />

• Contains a fourfold concentration of amino acids<br />

• Contains vitamins and additional components<br />

Stable Glutamine<br />

L-Glutamine is an essential amino acid and must be added to cell culture media. Since<br />

L-glutamine is a temperature sensitive component <strong>DMEM</strong> is also available with the stabilized form<br />

of L-glutamine (L-alanyl-L-glutamine). Cells don't have to be adapted to <strong>DMEM</strong> containing Stable<br />

Glutamine.<br />

Product Specifications<br />

CO 2 -Concentration, optimum 8.5 %<br />

pH 6.8 – 7.5<br />

Osmolality<br />

Endotoxin<br />

Cell Culture<br />

Sterility<br />

Shelf Life<br />

280 – 350 mOsmol/kg<br />

< 1 EU/ml<br />

tested<br />

tested<br />

without L-Glutamine 18 months<br />

with Stable Glutamine 18 months<br />

with L-Glutamine 12 months<br />

Storage<br />

+2°C to +8°C

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