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Medium Series<br />
<strong>DMEM</strong>, <strong>High</strong> <strong>Glucose</strong><br />
Cat. No. Volume<br />
<strong>DMEM</strong><br />
without L-Glutamine E15-009 500 ml<br />
<strong>DMEM</strong><br />
with L-Glutamine E15-810 500 ml<br />
<strong>DMEM</strong><br />
with Stable Glutamine E15-883 500 ml<br />
<strong>DMEM</strong><br />
with Sodium Pyruvate<br />
without L-Glutamine E15-011 500 ml<br />
<strong>DMEM</strong><br />
with Sodium Pyruvate<br />
with L-Glutamine E15-843 500 ml<br />
<strong>DMEM</strong><br />
without Phenol Red<br />
without L-Glutamine E15-047 500 ml<br />
<strong>DMEM</strong><br />
with D-Valine<br />
without L-Valine<br />
without L-Glutamine E15-055 500 ml<br />
<strong>DMEM</strong><br />
without Phenol Red<br />
with L-Glutamine E15-877 500 ml<br />
<strong>DMEM</strong><br />
without Calcium<br />
without L-Glutamine E15-078 500 ml<br />
<strong>DMEM</strong><br />
without <strong>Glucose</strong><br />
without L-Glutamine E15-079 500 ml
Medium Series<br />
Applications<br />
• For cultivation of most cell types<br />
• Usage is recommended with supplementation of 10% FBS<br />
Features<br />
• <strong>DMEM</strong> is a modification of the Eagle Medium with 4.5 g/l glucose<br />
• Contains a fourfold concentration of amino acids<br />
• Contains vitamins and additional components<br />
Stable Glutamine<br />
L-Glutamine is an essential amino acid and must be added to cell culture media. Since<br />
L-glutamine is a temperature sensitive component <strong>DMEM</strong> is also available with the stabilized form<br />
of L-glutamine (L-alanyl-L-glutamine). Cells don't have to be adapted to <strong>DMEM</strong> containing Stable<br />
Glutamine.<br />
Product Specifications<br />
CO 2 -Concentration, optimum 8.5 %<br />
pH 6.8 – 7.5<br />
Osmolality<br />
Endotoxin<br />
Cell Culture<br />
Sterility<br />
Shelf Life<br />
280 – 350 mOsmol/kg<br />
< 1 EU/ml<br />
tested<br />
tested<br />
without L-Glutamine 18 months<br />
with Stable Glutamine 18 months<br />
with L-Glutamine 12 months<br />
Storage<br />
+2°C to +8°C
Medium Series<br />
<strong>DMEM</strong>, Thermostable Media Formulation (mg/l)<br />
Inorganic Salts<br />
Calcium Chloride anhydrous 200.00<br />
Ferric(III)-Nitrate • 9H 2 O 0.10<br />
Potassium Chloride 400.00<br />
Magnesium Sulphate anhydrous 97.70<br />
Sodium Chloride 6400.00<br />
Sodium Dihydrogen Phosphate • H 2 O 125.00<br />
Sodium Hydrogen Carbonate 3700.00<br />
Amino Acids<br />
L-Arginine • HCl 84.00<br />
L-Cystine 48.00<br />
Glycine 30.00<br />
L-Histidine • HCl • H 2 O 42.00<br />
L-Isoleucine 105.00<br />
L-Leucine 105.00<br />
L-Lysine • HCl 146.00<br />
L-Methionine 30.00<br />
L-Phenylalanine 66.00<br />
L-Threonine 95.00<br />
L-Tryptophan 16.00<br />
L-Tyrosine 72.00<br />
Amino Acids<br />
L-Valine 94.00<br />
Vitamins<br />
D-Calcium-Pantothenate 4.00<br />
Choline Chloride 4.00<br />
Folic Acid 4.00<br />
Myo-Inositol 7.20<br />
Nicotinamide 4.00<br />
Pyridoxal • HCl 4.00<br />
Riboflavin 0.40<br />
Thiamine • HCl 4.00<br />
Other Components<br />
D-<strong>Glucose</strong> anhydrous in E15-080 1000.00<br />
D-<strong>Glucose</strong> anhydrous in E15-082 4500.00<br />
Phenol Red 15.00<br />
L-Serine 42.00