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DMEM, High Glucose

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Medium Series<br />

<strong>DMEM</strong>, <strong>High</strong> <strong>Glucose</strong><br />

Cat. No. Volume<br />

<strong>DMEM</strong><br />

without L-Glutamine E15-009 500 ml<br />

<strong>DMEM</strong><br />

with L-Glutamine E15-810 500 ml<br />

<strong>DMEM</strong><br />

with Stable Glutamine E15-883 500 ml<br />

<strong>DMEM</strong><br />

with Sodium Pyruvate<br />

without L-Glutamine E15-011 500 ml<br />

<strong>DMEM</strong><br />

with Sodium Pyruvate<br />

with L-Glutamine E15-843 500 ml<br />

<strong>DMEM</strong><br />

without Phenol Red<br />

without L-Glutamine E15-047 500 ml<br />

<strong>DMEM</strong><br />

with D-Valine<br />

without L-Valine<br />

without L-Glutamine E15-055 500 ml<br />

<strong>DMEM</strong><br />

without Phenol Red<br />

with L-Glutamine E15-877 500 ml<br />

<strong>DMEM</strong><br />

without Calcium<br />

without L-Glutamine E15-078 500 ml<br />

<strong>DMEM</strong><br />

without <strong>Glucose</strong><br />

without L-Glutamine E15-079 500 ml


Medium Series<br />

Applications<br />

• For cultivation of most cell types<br />

• Usage is recommended with supplementation of 10% FBS<br />

Features<br />

• <strong>DMEM</strong> is a modification of the Eagle Medium with 4.5 g/l glucose<br />

• Contains a fourfold concentration of amino acids<br />

• Contains vitamins and additional components<br />

Stable Glutamine<br />

L-Glutamine is an essential amino acid and must be added to cell culture media. Since<br />

L-glutamine is a temperature sensitive component <strong>DMEM</strong> is also available with the stabilized form<br />

of L-glutamine (L-alanyl-L-glutamine). Cells don't have to be adapted to <strong>DMEM</strong> containing Stable<br />

Glutamine.<br />

Product Specifications<br />

CO 2 -Concentration, optimum 8.5 %<br />

pH 6.8 – 7.5<br />

Osmolality<br />

Endotoxin<br />

Cell Culture<br />

Sterility<br />

Shelf Life<br />

280 – 350 mOsmol/kg<br />

< 1 EU/ml<br />

tested<br />

tested<br />

without L-Glutamine 18 months<br />

with Stable Glutamine 18 months<br />

with L-Glutamine 12 months<br />

Storage<br />

+2°C to +8°C


Medium Series<br />

<strong>DMEM</strong>, Thermostable Media Formulation (mg/l)<br />

Inorganic Salts<br />

Calcium Chloride anhydrous 200.00<br />

Ferric(III)-Nitrate • 9H 2 O 0.10<br />

Potassium Chloride 400.00<br />

Magnesium Sulphate anhydrous 97.70<br />

Sodium Chloride 6400.00<br />

Sodium Dihydrogen Phosphate • H 2 O 125.00<br />

Sodium Hydrogen Carbonate 3700.00<br />

Amino Acids<br />

L-Arginine • HCl 84.00<br />

L-Cystine 48.00<br />

Glycine 30.00<br />

L-Histidine • HCl • H 2 O 42.00<br />

L-Isoleucine 105.00<br />

L-Leucine 105.00<br />

L-Lysine • HCl 146.00<br />

L-Methionine 30.00<br />

L-Phenylalanine 66.00<br />

L-Threonine 95.00<br />

L-Tryptophan 16.00<br />

L-Tyrosine 72.00<br />

Amino Acids<br />

L-Valine 94.00<br />

Vitamins<br />

D-Calcium-Pantothenate 4.00<br />

Choline Chloride 4.00<br />

Folic Acid 4.00<br />

Myo-Inositol 7.20<br />

Nicotinamide 4.00<br />

Pyridoxal • HCl 4.00<br />

Riboflavin 0.40<br />

Thiamine • HCl 4.00<br />

Other Components<br />

D-<strong>Glucose</strong> anhydrous in E15-080 1000.00<br />

D-<strong>Glucose</strong> anhydrous in E15-082 4500.00<br />

Phenol Red 15.00<br />

L-Serine 42.00

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