PRODUCT INFORMATION SHEET - Biosearch Technologies

NIP-BSA (Bovine Serum Albumin)
PRODUCT INFORMATION SHEET
Base Catalog No.: N-5040
4-Hydroxy-3-iodo-5-nitrophenylacetyl hapten is conjugated to BSA protein through lysine by amide bonds.
Properties
Chemical Name: 4-Hydroxy-3-iodo-5-nitrophenylacetyl bovine serum albumin
Appearance: yellow powder
Absorption Maximum (Lambda Max): 430 nm
Extinction Coefficient at Lambda max: 5000/M/cm
Product Usage
Conjugation is determined at pH 8.5 by measuring NIP absorbance at 430nm, using the extinction coefficient of NIP= 5.0 x 10E+03.
The conjugation ratio is given as a molar ratio of NIP to BSA assuming the molecular weight of BSA is 60,000.
Store solution in aliquots at -20°C. Protect from light.
This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. When using PBS please refer
to the formulation below.
PBS Formulation:
8 g NaCl
0.2 g KCl
0.2 g potassium di-hydrogen phosphate (KH2PO4)
1.15 g di-sodium hydrogen phosphate (Na2HPO4)
Add to 1 L of water
Storage & Handling
Storage Conditions: +5°C, desiccated
Reanalysis Period: 3 Year(s)
Product Sizes
Catalog No
Catalog No
N-5040-10
N-5040-100
Size / Scale
Size / Scale
10 mg
100 mg

PRODUCT INFORMATION SHEET
Base Catalog No.: N-5040
NIP-BSA (Bovine Serum Albumin)
Quality Control
Test Name
Test Name
Immunochemical Conjugation Ratio to Protein
Immunochemical Solubility
Specifications
Specifications
Dissolves at 1 mg/mL in PBS Buffer; solution free of particulate
References
P. Garred, T.E. Michaelsen and A. Aase. The IgG subclass pattern of complement activation depends on epitope density and antibody
and complement concentration. Scand. J. Immunol. Vol 30, p379 (1989).
J.Z. Weinberger, M.I. Greene, B. Benacerraf and M.E. Dorf. Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. I.
Genetic control of delayed-type hypersensitivity by VH and I.A. region genes. J.E.M. Vol 149, p 1336 (1979).
E. Saksela, T. Imir and O. Makela. Specifically cytotoxic human and mouse lymphoid cells induced with antibody or antigen-antibody
complexes. J. Immunol. Vol 115, p 1488 (1975).
© 2013 Biosearch Technologies, Inc. Information subject to change without notice.
Rev. 4.0