RealTimeDesign Quick Start Guide - Biosearch Technologies

Q u i c k St a r t Gu i d eWelcome to RealTimeDesign Software, your free, easy to use, onlineexpert for probe and primer design. This quick start guide is designed tohelp you get started right away!Step 1: Create Account or Log InCreate Accountor Log inGo to: www.biosearchtech.com/realtimedesign. On the left side of thewebpage, click “Create Account”. Ifyou already have one, simply log in.Step 2: Start RealTimeDesignSoftwareStartRealTimeDesignSoftwareAfter logging in, click on “STARTREALTIMEDESIGN” to begin.Step 3: Dual-Labeled Probes orAmplifluor® PrimersDual-LabeledProbes orAmplifluor®Now select whether you want todesign dual-labeled probes orAmplifluor® primers. The followingsteps are the same for either choice.Enter Sequenceor NCBIAccession #EnterSequence HereStep 4: Enter Sequence or NCBIAccession NumberIn the sequence entry box, eitherpaste in your sequence or just typethe relevant NCBI Accession number(e.g. NM_204077). When finished,click the “Next” button.

Step 5: Choose InstrumentChooseInstrumentSelect the appropriate instrumentfrom the drop down menu, and thenpress “Next”.Step 6: Design Complete, ViewAssay DetailsView AssayExpand your target sequence andassay by clicking the “+” buttons.After reviewing your assay andoptionally selecting individualcomponents, click “Add To Cart”.Step 7: Select SpecificationsChooseSynthesis ScaleSelect your desired synthesis scaleand purification method for yourprobes and primers and review yoursequences. Click “Add To Cart”.Step 8 : Final ReviewFinal ReviewReview your cart, and then click“Checkout” to make your onlinepurchase.81 Digital Drive, Novato, CA 94949-5728 USA1.800.GENOME.1 (436.6631) +1.415.883.8400+1.415.883.8488 (fax)e-mail: info@biosearchtech.comwww.biosearchtech.com www.qpcrdesign.comRealTimeDesign and RTD are trademarks of Biosearch Technologies, Inc., Novato, CA. Biosearch’s RealTimeDesign software is powered by ProbeITy byCeladon Laboratories, Inc. PCR is a proprietary technology covered by several US patents, these patents are owned by Roche Molecular Systems Inc. andhave been sub-licensed by PE Corporation in certain fields. Depending on your specific application you may need a license from Roche or PE to practicePCR. Additional information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing at RocheMolecular Systems, 1145 Atlantic Avenue, Alameda, CA 94501 or Applied Biosystems business group of the Applera Corporation, 850 Lincoln CentreDrive, Foster City, CA 94404. In addition, the 5’ nuclease assay and other homogeneous amplifi cation methods used in connection with the PCR processmay be covered by U.S. Patents 5,210,015 and 5,487,972, owned by Roche Molecular Systems, Inc, and by U.S. Patent 5,538,848, owned by The Perkin-Elmer Corporation. TaqMan is a registered trademark of Roche Molecular Systsems, Inc. Amplifluor is a registered trademark of Serologicals Corp.