TGen Tissue Microarray Center | - Translational Genomics Research ...

TGen Tissue Microarray Center |
Clean, high quality TMAs optimized
for the detection of your specifi c targets
The TGen TMA Center plays a
critical role in the translational
research process through standardized
collection and organization
of clinical tissues. The TMA format
offers high throughput capacity for
molecular analyses and provides
inherent assay standardization.
Active collaborations with imaging
experts and computer programmers
are an integral component
of the TMA Center’s efforts to
continually extend the applications
of TMA technology. Most notable
of these was a two-year collaborative
development of an automated
fluorescence in situ hybridization
(FISH) spot counting program. This
highly interactive program was
designed to enumerate probe
signals after hybridization of
specific DNA sequences. In situ
hybridization (ISH) applications
using DNA or RNA probes remains
an active part of Dr. Hostetter’s
research.
About the Center Director
Dr. Galen Hostetter is the
Director of the Tissue Microarry
(TMA) Center at TGen and an
Associate Investigator at TGen.
Dr. Hostetter is a board-certified
pathologist and has significant
expertise and experience in
constructing tissue microarrays
and the molecular assays of IHC
and FISH on TMA slides.
Board Certifications:
• Diplomate of the American Board
of Pathology, Certified in Clinical
Pathology, September 2001.
• Board Eligible, Clinical Genetics
and Molecular Genetics, American
Board of Medical Genetics, June
2002.
http://www.tgen.org/
About TGen Tissue Microarray
The TGen Tissue Microarray Center offers expert design and
construction of tissue microarrays (TMA) for the analysis of hundreds
of precisely arrayed tissue specimens on one microscope slide,
resulting in unparalleled assay standardization. The Center adheres
to the highest level of quality control to maximize extraction of
biological information from archival tissue blocks.
Common Applications
of Tissue Microarray
in Cancer Research include:
• Validation of molecular
markers in clinical specimens
• Discover potential diagnostic
markers
• Optimize antibody screening
and staining
• Analyze the frequency
of a molecular alteration in
different tumor types
Why use the TGen Tissue
Microarray Center
• High throughput capacity
• Highly cost competitive
• Rapid turnaround time
• Highest level of quality control to
maximize extraction of biological
information from archival tissue blocks
• Specialized sectioning technique to provide TMA slides with
minimal background using water-floated sections - thus
no glue residue!
• Consistent and interpretable staining results
• Flexible service to meet your individual needs
• Center director is a board-certified pathologist with NIH fellowship
training and extensive experience in TMA design and construction
Technology
The Chemicon Advanced Tissue Arrayer (ATA100) provides precise and
accurate sample extraction and TMA construction. The IHC staining is
optimized with advanced epitope retrieval methods utilizing a capillary
system manual stainer (Thermo Shandon).
The TMA Center offers high quality digital imaging with the Zeiss
Axioplan2 microscope and fully integrated AxioVision 4.1 software. The
recently released Zeiss TMA module offers automated TMA slide image
acquisition, storage and retrieval. This permits rapid and uniform IHC
scoring on TMA slides, including high resolution digital image files for
each tissue spot.
®

TGen Tissue Microarray Center |
Clean, high quality TMAs optimized
for the detection of your specifi c targets
Services
TGen offers the following TMA services:
• Design and construction of tissue microarrays (with expert
sectioning on standard glass slides) for investigators with
parrafin tissue block collections
• Access to high quality “tester” TMA slides for optimization of
molecular assay protocols
• Tumor specific TMA slides are under development for IRB approved
protocols
• Immunohistochemistry (IHC) and fluorescent in situ hybridization
(FISH) services adapted to paraffin tissue sections
* Available in October 2004 – in situ hybridization assays!
• High-throughput analysis of protein and DNA targets in formalin
fixed paraffin embedded tissue section
• Specialized antibody optimization services for immunostaining on
formalin fixed paraffin embedded tissue sections
Our service can include any combination of the following:
1. literature search for application guidelines
2. choice goat, rabbit, mouse kits
3. chromagen choice: Dako DAB, Vector Red
4. expert consultation with TGen investigators
Fees
Please contact us directly for a complete fee schedule of all of our
services. The following pricing is for not-for-profit institutions and is to
be used as a guideline only as each investigator’s project is unique.
Array Construction and Design
0.6 mm Core - $5,600 ($28 TMA slide)
1.0 mm core - $4,100 ($20 TMA slide)
Antibody tester slide - $90 each
IHC staining only:
• 1-6 slides: $465.00
• 7-12 slides: $645.00
• Greater than 12 slides: contact us for pricing
Questions
For antibody optimization,
immunohistochemistry services
or more information, please
contact Galen Hostetter, MD,
Director, TGen Tissue Microarray
Center.
Phone: (602) 343-8810
E-mail: ghostetter@tgen.org
TGen Tissue Micorarray
1255 W. Washington Street
Tempe, AZ 85281
Antibody optimization: (includes 6 slides post)
• low complexity: $680.00
• high complexity: $1,800.00
Note: Researcher provides IHC antibody to the TGen TMA Center.
Results
Tissue Microarray data is collected using an imaging station comprised
of a Zeiss microscope and Spot Insight digital camera for high
resolution imaging in both brightfield and florescent applications. We
can supply you with a report that includes digital files of your images.
For more information, please visit: www.tgen.org