Samba Sensors Background - Harvard Apparatus UK

Samba Preclin
Samba 200
Product Presentation

Samba Preclin 420/360 Transducer
Ultra-miniature fiber optic pressure sensor –
no bigger than a grain of salt on a hair!
Highly sophisticated, accurate, fast and sensitive instrument
designed to measure pressure in gas or liquid.
Constructed with patented state-of-the-art micro mechanic and
fiber optic technology.
A first-class tool for building knowledge and solving problems in
numerous application areas.
Samba Preclin moves science into new
territories!
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Samba Sensors’ Technology

The Sensor
Sensor
Low pressure ∅ 0.42mm
High pressure ∅ 0.36mm
Optical fiber
∅ 0.25mm
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Measuring Hydrostatic Pressure
Space filled with
GAS or LIQUID
Hydrostatic pressure
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The Sensor on a Finger Tip

X-ray Visibility
Radiopaque coating
∅ 0.40mm
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Samba Products

Main Products
Ultra-miniature optical pressure
transducers in different versions.
For preclinical and industrial use.
Control units with analog and serial ports.
Samba Data Collection software for PC.
For research use.
Not FDA approved
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Preclinical Transducers
• Samba Preclin 420 LP
• Miniature optical pressure transducers
for research in vitro and in vivo. Low
pressure range (-50 to +350 mbar).
Also with radiopaque coating. 4m long.
• Samba Preclin 420/360 MR
• Miniature optical pressure transducers
for research in vitro and in vivo. Extra
long for use in MRI facilities. Low and
high pressure ranges. 10m long.
• Samba Preclin 360 HP
• Miniature optical pressure transducers
for research in vitro and in vivo. Three
high pressure ranges (-0.1 to 5 bar, -
0.1 to 10 bar, -0.1 to 17 bar). Also with
radiopaque coating. 4m long.
Protective sensor covers are available
for special applications
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Samba Preclin Transducer
• Pressure can be measured in very small cavities or directly
in the tissue of small (or large) animals.
• The ultra-miniature size minimizes insertion trauma.
• Insensitive to EM radiation and RF fields (MRI scanner,
microwaves etc).
• Direct measurements with very high precision are made at
the sensor location.
• The transducer does not affect the measuring environment
itself.
• Low risk – no electricity or bio-hazardous material.
• Long transducer possible (up to 10m).
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Samba 201 Control Unit
• One fiber-optic port
• Battery operation
• Internal memory
• Absolute or relative measurement
• Internal barometer
• 3.2 inch display
• USB 2.0, analog and RS-232 ports
• Analogue output: 0-5V
• Sampling frequency: 1 Hz – 40 kHz
• Resolution: 0.1 mbar (~1mm H 2 O)
• Numerical resolution: 15 bit
• Numerical res.: 0.1 mbar
• Operating temp.: 15 – 35 C
• Size: 215x110x45mm
(8.5x4.3x1.8 in)
• Weight 950g (2.1 lbs)
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Samba 202 Control Unit
• Two fiber-optic ports
• Battery operation
• Internal memory
• Absolute or relative measurement
• Internal barometer
• 3.2 inch display
• USB 2.0, analog and RS-232 ports
• Analogue output: 0-5V
• Sampling frequency: 1 Hz – 40 kHz
• Resolution: 0.1 mbar (~1mm H2O)
• Numerical resolution: 15 bit
• Numerical res.: 0.1 mbar
• Operating temp.: 15 – 35°C
• Size: 215x110x45mm
(8.5x4.3x1.8 in)
• Weight 950g (2.1 lbs)
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Fast and Simple Start-Up Procedure
1. Insert the Samba transducer
The transducer is factory calibrated - No further calibration is needed!
2. Turn on your Samba 200 Control Unit
Allow internal circuitry temperature to stabilize (1-2 minutes)
3. Set the “zero baseline” against ambient air pressure
The transducer is factory calibrated - No further calibration is needed!
Simply follow instructions on the screen (‘ZERO’ or ‘PREVIOUS’)
4. If needed set measurement rate, Absolute/Relative mode,
pressure unit etc. or load pre-defined Profile
5. Connect to PC or Data Acquisition System (DAS)
When connected calibrate DAS by pressing ‘ANALOG CALIB.’
6. Remove protective cover, apply sensor in object and start
measuring!
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Samba vs. Competition
The Samba system…
1. …has a simple and fast start-up procedure (~3 min) – just Plug’n Play!
2. …is factory calibrated and doesn’t need further calibration, only an
automatic “zero baseline” set against the atmospheric pressure.
3. …is extremely fast (up to 40 kHz data sampling rate) and can record
high-speed trauma events, for example ICP events caused by collisions.
4. …is fully MRI compatible and completely insensitive to any electromagnetic
waves radio waves or electrical noise. Real-time measurements
and simultaneous recording of both visual and physiological effects of for
example a drug is possible. Gating (MRI scanner triggering) for improved
MR image quality and movie sequences is also feasible.
5. …can measure pressure both in gas and liquid, which allows a very
broad use.
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Examples of
Applications

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Inserting Transducer into a Mouse Heart

Monitoring Pressure in a Mouse Heart
Right Ventricular Pressure
also possible!
Transducer insertion
Am J Physiol Heart Circ Physiol, Vol. 285, pp. H2233-H2239, 2003
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Pulse Wave Analysis (PWA)
• PWA, i.e. the analysis of the pulse
pressure wave, is a method for
studying vascular stiffness
• PWA provides valuable
information for the
characterization of cardiovascular
disease, atherosclerosis and
endothelial function
• The analysis is preferably made
on a number of pulse wave cycles
selected from a specific time
period
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Parameters in Samba PWA Software
• Pdiast = end-diastolic pressure
• Psyst = inflection point in systole
• Paug = max pressure during entire cycle
• RRint = heart cycle length
• Tsyst = time between pdiast and psyst
• PP = paug – pdiast
• Taix = time from pdiast to paug
• Aug = Paug – Psyst
• AIx (augmentation index) = Aug/(Paug-Pdiast)
• Further parameters related to the Dicrotic Notch
will be available in future software version
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Transducer Introduction into Rat Tail Artery

Triggering the MR Scanner (Gating)
100.00
90.00
Respiration
80.00
Triggering point: Exhalation & min. diastolic blood pressure
70.00
Pressure [mmHg]
60.00
50.00
40.00
30.00
20.00
10.00
Sharper MR images
0.00
& movie sequences!
0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00
Time [s]
B. Bjelke, MD, PhD, Karolinska Institute, Sweden 2003
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Gating on Samba Signal (IBP)
• Image of rat heart
• MRI scanner triggered
on rat tail IBP signal
• Advantages
• Improved image quality
(less artifacts than
ECG)
• Real-time IBP
monitoring
• Volume & CO
• Movie sequences
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MRI Movie Sequence

Intracranial Monitoring on a Rat
Samba transducer
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ICP in Fluid Percussion Model
3500
3000
Intracranial Pressure
Pressure [mbar]
2500
2000
1500
Water Pressure
1000
500
0
0,00 0,10 0,20
Time [s]
Acta Neurochir Vol. 147, No. 7, pp. 775-780, 2005
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Rabbit CNS Trauma Model
Eur J Neurosci. Vol. 21, No. 10, pp. 2876-82, 2005
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Rabbit CNS Trauma Model
1.5
1.4
30000
1.3
-20000
1.2
Pressure [bar]
1.1
1
0.9
-70000
-120000
Rot. acc.(rad/s^2)
0.8
0.7
-170000
0.6
0.5
-220000
0.08 0.085 0.09 0.095 0.1 0.105 0.11
Time (s)
Occipital lobe pressure transducer Parietotemporal lobe pressure transducer Rotational acceleration
Eur J Neurosci. Vol. 21, No. 10, pp. 2876-82, 2005
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Spinal Disc Measurements
Proceedings SPIE 3570, 1998
Samba transducer inserted
into disc via a cannula
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Proceedings SPIE 3570, 1998

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Urodynamic Investigation
Transurethral introduction of transducer into bladder

Urodynamic Investigation
Transurethral introduction of transducer into bladder
Courtesy of Prof. Ulla Sillén, Dept. of Pediatrics
Sahlgrenska University Hospital, Sweden
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Airway Pressure Monitoring
Endotracheal tube
Samba sensor
Ventilator pressure sensor
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Pressure Measurement in Pediatric ETT
Transducer inserted through tube into lower trachea
Relative pressure [cmH2O]
30
25
20
15
10
5
0
Samba
Respiratory
1 51 101 151 201 251 301
Samples [25 Hz]
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Intra-folliclular Pressure
(during open surgery)
Time [sec]
P Dahm-Kähler et al. Poster Läkarstämman, 2004
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Clinical Samba Projects
1. Urodynamic investigations
2. Respiratory pressures
3. Intracranial pressures
4. Intervertebral disc pressure (spinal surgery)
5. Cardiovascular blood pressures
6. Gastro-intestinal pressures
7. Intra-follicle pressure (gynecology)
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Samba’s unique offering
High-precision pressure measurements can be
performed where never before possible.
New science and knowledge can be revealed
leading to more publications.
APPLICATION AREAS:
- Heart and Circulation
- Brain and Nervous System
- Lungs and Breathing
- Gastro-intestinal System
- Kidney and Urinary System
- Reproduction System
- Muscles
- Diseases like Cancers, Stroke, Spinal
Disorders and more
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Contact
Harvard Apparatus, Ltd
Fircroft Way,
Edenbridge
Kent TN8 6HE
England
United Kingdom
Phone: (44) 1732-864001
Fax: (44) 1732-863356
E-Mail: sales@harvardapparatus.co.uk
Internet: www.harvardapparatus.co.uk
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