peqGOLD Protein-Marker X Rabbit - Peqlab

Data sheet
peqGOLD Protein Marker X Rabbit
Lot No.
________
Usage Standard gel application 5 – 10 μl/lane
Cat. No. 27-2710 1 x 250 μl
27-2711 5 x 250 μl
Description
The peqGOLD Protein Marker X Rabbit is a mixture of 8 recombinant and highly purified proteins.
Each protein contains an IgG-binding site and is proportioned to yield comparable gel electrophoresis
and Western blotting band intensities. The ladder also contains a pink tracking dye in the buffer for
monitoring electrophoresis and transfer in Western blotting. This formulation is appropriate for use
with most rabbit and other non-mouse polyclonal antibodies.
The marker can be detected using chemiluminescence, fluorescence, chromogenic or other detection
systems.
8 fragments 20, 30, 40, 50, 60, 80, 100 and 150 kDa
Note
All proteins resolve into clearly defined, sharp bands in the range of 20 kDa to 150 kDa when
analysed by SDS-PAGE, and can be visualized by Coomassie Brilliant Blue R250 staining.
As each protein contains an IgG-binding site protein bands can be detected in Western blotting by
specific monoclonal antibodies followed by HRP-conjugated secondary antibody.
Application
Denaturing Polyacrylamide gels (SDS-PAGE)
• Thaw marker at room temperature or heat at 37 – 40 °C for a few minutes.
• Do not boil!
• Vortex gently and apply 1 – 10 μl marker directly to the gel. Start the gel run.
• Stop electrophoresis when the pink dye front has reached the bottom of the gel.
• After electrophoresis visualize proteins by Coomassie staining or Western Blot detection.
• Suggested loading volumes for various detection methods are shown in the following table:
Detection system
Loading volume (μl)
Colorimetric 10
Fluorescent 5 – 10
Chemiluminescence 1 – 10
ECL Substrate 5 – 10
Super Signal West Pico Substrate 5 – 10
Super Signal West Dura Substrate 2 – 5
Super Signal West Femto Substrate 1 – 5
PEQLAB_v0314_E
info@peqlab.com · www.peqlab.com

peqGOLD Protein Marker X Rabbit
Note
The marker was optimized for staining using Coomassie
Brilliant Blue R-250, but can also be visualized by other
staining methods (e.g. silver staining). As this method is
10 to 100fold more sensitive than Coomassie staining, the
amount of applied marker should be reduced accordingly.
peqGOLD Protein Marker X contains SDS and is therefore
not recommended to be used in native polyacrylamide gels
for determing native molecular weights of proteins.
If the marker shall be diluted use reducing loading buffer.
Chemiluminescent detection of Protein Marker X Rabbit:
The marker was separated by electrophoresis and transferred
to a nitrocellulose membrane. Proteins were detected by
hybridization with monoclonal rabbit antibodies followed by
HRP-conjugated donkey-anti-rabbit secondary IgG, using ECL
Western Blotting substrate.
• Shipment: Blue ice
• Storage: –20 °C
• Stability: __________
PEQLAB_v0314_E
info@peqlab.com · www.peqlab.com