Nucleation and seeding in protein crystallization - Hampton Research

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Matrix Seeding: Automated seeding • Screen Setup • Use Oryx robot with seeding function • Dispensed drops 0.6µl drops containing 0.3µl protein 0.2µl reservoir solution 0.1µl seed stock
Screening for initial crystallization conditions • ~ 300 “precipitating agents are tested in the first screens • 300nl of protein are mixed with 300nl of precipitating agent
Page 1 and 2: Nucleation and seeding in protein c
Page 3 and 4: Growing protein crystals We have 2
Page 5 and 6: Classes of proteins (an oversimplif
Page 7 and 8: Protein very soluble, no nucleation
Page 9 and 10: Nucleation • The growth of protei
Page 11 and 12: Drop set up (mix) Mixing drops
Page 13 and 14: Drop size • D. G. Georgieva, M. E
Page 15 and 16: Different seeding methods
Page 17 and 18: Different seeding methods: Seed bea
Page 19 and 20: Different seeding methods: Heteroge
Page 21 and 22: The first artifial snow crystals
Page 23 and 24: Cause and effect/proof of concept
Page 25 and 26: Other nucleation agents • McPhers
Page 27 and 28: Important publication Acta Cryst. (
Page 29: Microseed Matrix Seeding: preparati
Page 33 and 34: Microcrystalline droplets/phase sep
Page 35 and 36: You may generate more false positiv
Page 37 and 38: Using UV to identify protein crysta
Page 39 and 40: Example of confirmed screen hit
Page 41 and 42: CYS protease • Starting crystals
Page 43 and 44: Metallo Protease Only one crystal g
Page 45 and 46: Eliminate twinning Result: Twinning
Page 47 and 48: Fab complexes, another powerful cry
Page 49 and 50: Conclusions and questions • In pr
Page 51: Backup

Nucleation and seeding in protein crystallization - Hampton Research

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