Nucleation and seeding in protein crystallization - Hampton Research

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Using spherulites 4 deg Result: New conditions New crystal form Complex structure solved of natural compound
Fab complexes, another powerful crystallization aid matrix seed Fab complexes combined with matrix seeding, gives crystals of target protein for the first time.
Page 1 and 2: Nucleation and seeding in protein c
Page 3 and 4: Growing protein crystals We have 2
Page 5 and 6: Classes of proteins (an oversimplif
Page 7 and 8: Protein very soluble, no nucleation
Page 9 and 10: Nucleation • The growth of protei
Page 11 and 12: Drop set up (mix) Mixing drops
Page 13 and 14: Drop size • D. G. Georgieva, M. E
Page 15 and 16: Different seeding methods
Page 17 and 18: Different seeding methods: Seed bea
Page 19 and 20: Different seeding methods: Heteroge
Page 21 and 22: The first artifial snow crystals
Page 23 and 24: Cause and effect/proof of concept
Page 25 and 26: Other nucleation agents • McPhers
Page 27 and 28: Important publication Acta Cryst. (
Page 29 and 30: Microseed Matrix Seeding: preparati
Page 31 and 32: Screening for initial crystallizati
Page 33 and 34: Microcrystalline droplets/phase sep
Page 35 and 36: You may generate more false positiv
Page 37 and 38: Using UV to identify protein crysta
Page 39 and 40: Example of confirmed screen hit
Page 41 and 42: CYS protease • Starting crystals
Page 43 and 44: Metallo Protease Only one crystal g
Page 45: Eliminate twinning Result: Twinning
Page 49 and 50: Conclusions and questions • In pr
Page 51: Backup

Nucleation and seeding in protein crystallization - Hampton Research

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