here - BioCision

Product Overview
April 2014

About BioCision
Page
“The lack of standardized, high-quality biospecimens has been
widely recognized as one of the most significant roadblocks to the
progress of cancer research.”
- NIH / NCI / BBRB
Develop products to address unmet standardization needs in preanalytical
sample handling, processing, storage and transport
BioCision products improve pre-analytical standardization of
temperature-sensitive bio-medical samples:
• Day to day
• User to user
• Lab to lab
• Site to site
2

Consistent. Reproducible. Standardized.
Sample Temperature Management
CoolRack ® and CoolBox for
reproducible preparation of
temperature-sensitive samples
Cell Cryopreservation
CoolCell products for
standardized, controlled-rate cell
freezing
Intuitive design.
Proprietary advanced thermal management technology.
Page 3

Page 4

Current methods are inconsistent and
non-reproducible
mmm
Cooling samples in ice or gel-filled cooling blocks introduces a
variety of problems.
Ice cooling
• Variable sample temp
• Contamination
• Wet, illegible labels
• Non-reproducible method
Gel-filled coolers
• Highly variable sample temp
(edge effect)
• Short cooling duration
• Cannot use while re-charging
Page 5

CoolProducts provide consistent and
reproducible sample cooling and freezing
Using a thermo-conductive CoolRack ® module as an interface
between the ice and sample provides a consistent and reproducible
cooling method.
Eliminating ice with a CoolBox system further minimizes the risk
of sample contamination and provides hours of consistent and
reproducible ice-free cooling or freezing.
Page 6

CoolRack modules ensure reproducible
cooling and thawing profiles
Performance test: A temperature probe was placed into a 2.0 mL cryogenic vial containing 1.0 mL of water. The tube was
inserted into a CoolRack CF45 thermo-conductive module. The module was placed onto a ThermalTray platform in a 37°C water
bath and allowed to equilibrate. The CoolRack CF45 module was then removed and placed onto dry ice and equilibrated to -78°C
(0 - 15 minutes) and then returned to the water bath to re-equilibrate to 37°C (15 - 30 minutes). This experiment was repeated five
consecutive times and temperature profiles were recorded.
Conclusion: The CoolRack CF45 module showed identical cooling profiles and phase transition (orange circles) over five
consecutive freeze-thaw cycles.
Page 7

CoolRack, CoolSink, ThermalTray:
Compatible with broad temperature range
-150˚C -78˚C 0˚ to 4˚C 37˚C +
Liquid Nitrogen Dry Ice Ice Water Bath
• Adapts from ambient (25 o C)
to approx. -150 o C in 12-14
minutes
• Samples are upright and
organized as they flash freeze
• No direct contact between
sample and LN 2
• Reproducible method
• Adapts from ambient (25 o C)
to -78 o C in 5-7 minutes
• No need for ethanol – cost
savings, no hazardous waste,
no “sunk” tubes
• Samples are upright and
organized as they snap-freeze
• Same freezing rate as direct
immersion into dry ice or
alcohol slurry
• Reproducible method
• Adapts from ambient (25 ç C)
to 100°C).
Samples protected from direct contact with temperature source.
Page 8

CoolRack, CoolSink, ThermalTray:
Accommodates variety of tubes and plates
CoolRack ® tube modules
• Including SBS-format modules
CoolSink ® plate modules*
• Including SBS-format modules
ThermalTray platforms*
• For supporting CoolRack and CoolSink
modules in liquid
* Patented
Page 9

CoolBox: Ice-free workstations
CoolBox XT & 2XT CoolBox MP CoolBox 30 XT Starter
Holds Tubes
Holds Plates
Page 10

CoolBox: How it works
CoolBox XT and 2XT CoolBox MP and 30
Lid
CoolRack or CoolSink
sample module
Cooling Cartridge
(Pre-frozen in -20˚C
freezer)
Base
Simply pre-freeze the Cooling Core or cartridge in a -20°C freezer, place it in the base
of the CoolBox, and rest a CoolRack or CoolSink module on top.
Page 11

CoolBox and CoolRack: Accessories
TruCool ® ice pans and buckets
• Made of non-toxic EVA foam
• Non-skid bottom, insulative and non-sweating
• 5 sizes, 7 colors
TruCool ® microfuge tubes and cryogenic vials
CryoCeps cryovial grippers
• Microfuge tubes feature patented “pull back” cap ensures
thumb never passes over open top minimizing
contamination risk
• Cryogenic vials feature unique thermally-fused gasket
(replaces O-ring) and bar-codes for secure identification
• CryoCeps grippers protect fingers from handling frozen
vials and tubes
CoolProbe temperature monitors
• Monitor sample temperature while using CoolRack and
CoolSink modules in ice
• Fitted probes for CoolRack M, CoolRack CF(T)
• Universal probe for other CoolRack and CoolSink modules
Page 12

CoolBox and CoolRack:
Use for a variety of applications
If you currently use an ice bucket, gel-filled coolers,
refrigerators, freezers, or water bath, CoolBox and CoolRack
products will improve your processes. Ideal for:
• Tissue / Cell culture
• Tissue dissections & snap-freezing
• Cell culture preparation and thawing
• Protein / Proteomics
• Protein isolation, sonication, fractionation
• Trypsin digestion
• Molecular biology
• PCR preparations
• RNA isolation
• Reagent cooling
• Microbiology
• Bacteria and virus freezing
Visit www.biocision.com/application-summary
for application assistance.
Page 13

Page 14

CoolCell ® : Alcohol-free cell freezing
CoolCell
CoolCell SV2
CoolCell FTS30
CoolCell 5ml LX
CoolCell LX
For a variety of tubes and capacities.
The new standard for passive cell cryopreservation.
Page 15

CoolCell: How it works
Passive controlled-rate cell freezing container
• Replaces alcohol-based containers such as “Mr. Frosty”
• Simply fill with vials and place -80 o C freezer
• Radially-symmetric thermal exchange design ensures
uniform cooling profiles to all vial positions
• Composed of high-density materials used in aerospace
industry for lightweight & insulative properties
• Highly thermo-conductive alloy core
• Models include conduction and/or micro-convection air
flow control
• Equal or better results than programmable step-down
freezers and alcohol-based freezing containers
Patent Pending
Replaces “Mr. Frosty” and programmable freezers.
No alcohol. No on-going maintenance or cost. No hazardous waste disposal.
“Mr. Frosty” is a trademark of Thermo Fisher Scientific.
Page 16

CoolCell containers ensure reproducible
freezing profiles
Five consecutive freezing runs show extremely consistent thermal profiles.
Page 17

CoolCell vs. “Mr. Frosty”
CoolCell Isopropanol Container
Consistent freezing rate
• All vials have uniform freezing profiles
No isopropanol
• No maintenance
• No pre-cooling
• No hazardous waste
No hidden cost
• Once you buy it, you’re done paying for it!!
Easy to use
• Cap removes easily when frozen
• Not cold to the touch when frozen, no gloves
needed
• Ready for reuse after 5 minute warm-up time
Minimal impact on freezer
• 1/3 the heat impact on freezer; nearby samples
remain unharmed
Inconsistent freezing rate
• Isopropanol degradation ensures variability
• Two circles of vials results in further non-uniform
heat removal
Requires Isopropanol
• Track uses so can replenish IPA every 5 th use
• On-going maintenance
• Spill risk
• Pre-cool in refrigerator (safety issue)
> $350 per unit per year to maintain properly
• Isopropanol expense
• Hazardous waste disposal costs
Difficult to handle
• Cap is difficult to open when frozen, unit is slippery
(cells are warming up)
• Protective glove required when handling frozen unit
• Unit is cold, slippery, breakable
Impacts local freezer area
• Large heat capacity removed from alcohol impacts
nearby samples
Page 18

CoolCell: Compatible with many cell types
Cell Lines
• LnCap, HTB77, A549, others
• Data and testing done by Lonza (Germany)
Primary Cells
• Variety of primary cells tested by labs at UCSF and ZenBio
Stem Cells and Cell Therapy Products
• Testing and data performed by USCF (Bluestone lab), TxCell and others
See CoolCell data at:
http://www.biocision.com/resources/coolcell-performance-data
Viability and post-thaw functionality
as good or better than “Mr. Frosty” and programmable freezers.
Page 19

Cell Cryopreservation: Accessories
XT Starter ice-free cooling platform
• Ideal for use in the hood where ice is forbidden
• XT Starter and CoolRack modules provide compact, icefree
cooling for cryogenic and cell therapy vials
TruCool ® Hinged CryoBoxes
• Patented hinged lid ensures lid is always put on
correctly, keeping markings in synch with cryovials
• Boxes for 1ml & 2ml vials, and for taller vials
• 6 bright colors
• With or without LN 2 drain holes
TruCool ® cryogenic vials
CryoCeps cryogenic vial grippers
• Patented fused gasket layer on vial closure ensures
leak-proof seal
• Bar-coded vials for secure sample identification
• CryoCeps* protect fingers when handling frozen vials
* Patent pending
Page 20

Thank you!
For questions, contact us at: info@biocision.com
© 2014. BioCision, LLC. All rights reserved. BioCision, Standardizing Samples, CoolRack, CoolSink, ThermalTray,
CoolBox, CoolCell, TruCool, CoolProbe, CryoCeps are designated trademarks owned by BioCision, LLC..