Satish P. RamachandraRao, M.Sc., Ph.D. - Division of Nephrology ...

Satish P. RamachandraRao, M.Sc., Ph.D.
Assistant Professor of Medicine,
Director, Biomarkers Laboratory,
Co-Director, Bioanalytical Core, O’Brien Center for Acute Kidney Injury Research,
Divn. Nephrology – Hypertension, School of Medicine
Center for Promotion of Maternal Health and Infant Development,
Divn. of Dysmorphology & Teratology,
UC San Diego Dept. of Pediatrics,
Clinical Teaching Facility Bldg C; Ste 205B
214 Dickinson St Mail Box 8324 San Diego, CA 92103.
619-543-7298 (Pref. Work & Direct) 619-471-0407 (Lab)
619-543-7769 (Fax) satishrao@ucsd.edu (email)
http://nephrology.ucsd.edu/faculty/profiles/RamachandraRao.shtml
OBJECTIVES
To lead and conduct translational biomedical research aimed at
understanding different stages of disease physiology - manifestation,
progression and regression, using biomarkers developed by proteomic,
metabolomic and other biochemical methods.
EDUCATION
2004 Thomas Jefferson University; Philadelphia, Pennsylvania.
Post-Doctoral Fellowship, Biomarkers in Diabetic Nephropathy.
2002 Indian Institute of Science; Molecular Biophysics Unit, Bangalore, India
Post-Doctoral Fellowship.
1999 Christian Medical College Hospital, Vellore, India.
Dissertation: “Protein Phosphorylation in Human & Monkey Cerebellum
and the Neurotransmitter Receptor Triad from the Monkey Brain.”
Advisor: Ailam S. Balasubramanian, Ph.D.
1993 Bangalore University; Bangalore, India.
Master of Science (Specialization: Biochemistry)
1991 Bachelor or Science (Triple Major: Chemistry, Botany, Zoology)
EXPERIENCE
2012-till date
Director; Biomarkers Laboratory,
Co-Director, Bioanalytical Core, O’Brien Center for AKI Research
UC San Diego
My activities at the NIH-funded O’Brien Center are:
• To develop and direct application of AKI research tools
• To provide cutting edge research expertise to investigators within and outside UCSD
involved in clinical and translational research in AKI
• To impart education and training to young researchers in Translational Research in AKI
in various etiologies
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2007-till date Assistant Professor of Medicine; Department of Medicine, Division of
Nephrology-Hypertension, University of California, San Diego.
My role as a faculty of the school of Medicine at UC San Diego is tripartite: a) Research
Scholarship, b) Teaching and c) University Services.
a. Research Scholarship
• Development of Translational Research Focused on Exosomes
o Exosome purification technology development: led the interdisciplinary
group comprising Nephrology–Biochemical Genetics divisions that
successfully invented a soft method to isolate and purify exosomes from
any given bio-fluid source.
o Demonstrated that the non-invasively obtained human urinary exosomes
that were isolated and purified using the above methodology could be used
as an excellent source of biomarkers tracking the physiologic status of the
cell/organ that the exosomes were derived from. As a result, urine
biomarker changes accompanying disease states have been characterized
with specific reference to progression of pregnancy in diabetic background
(Type-2 diabetic pregnancy) and progression of diabetes in the pregnancy
background (gestational diabetes). Technology extendable to study any
given disease biology
• Development of Translational Research Focused on Proteomics
o Developed Proteomics and Metabolomics discovery platforms to
understand selective pathway enrichment phenomena in general health,
and how they get altered in disease.
o Systems Biology (SB) approach to not only tease out meaningful data
from proteomics/Metabolomics platforms, but also understand how
different pathways are affected in diabetic kidney disease. Primary and
corresponding author on original research proteomics articles published in
prestigious Journal of American Society of Nephrology (JASN) and
Expert Reviews in Endocrinology and Metabolism (EREM).
o The diabetic kidney proteomics/SB abstract we submitted to the 9th
International Human Proteome Organization (HUPO) meeting held in
Toronto, Canada (2009), was selected for oral presentation in the very
prestigious HKUPP (Human Kidney and Urine Proteomics Project)
workshop that was held as part of the 9th HUPO meeting. My talk was
(the only talk selected from USA) one among the only 7 oral presentations
selected for presentation in this workshop that featured cutting edge
research applications addressing pathophysiology of kidney disease.
• Development of Translational Research Focused on Urinary Biomarkers
o Demonstrated the effect of HIV (human immunodeficiency virus)
infection on biology of THP (Tamm-Horsfall Protein), the most abundant
protein in the healthy human urine synthesized exclusively in the renal
tubules.
o Demonstrated that the Leptospirosis infection results in different host
responses in male and female infected rats. Neutral amino peptidase sorts
into urine exosomes in the rat kidney tubules in response to leptospirosis
infection.
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. Teaching: I have been involved in both within and outside the classroom teaching:
• Regular Classroom Teaching
SPPS 240 Histology course at the Skaggs School of Pharmacy and
Pharmaceutical Sciences, UC San Diego. My teaching is well-received
(Continuously rated 4.3/5.0 from 2008 - 2013) by students of Pharmacy.
• On Bench Translational Laboratory Research Training
• One of my teaching foci has been the under-represented/under-privileged
minority section. I helped an under-privileged minority section undergraduate
research fellow to obtain a research scholarship from Amgen to work during
the summer of 2008 in my laboratory. This student then went on to attend the
2008 national meeting for the Society for Advancement of Chicanos and
Native Americans in Science (SACNAS) conference held in Salt Lake City
Utah (October 9-12, 2008). The poster exhibited by this student not only got
selected for oral presentation, but also won the best presentation award. Thus
my commitment to encourage the students continually to raise the bar and aim
at extraordinary achievement has been recognized outside the UC San Diego
community as well.
• I have been involved in teaching outside the classroom in various capacities in
the last few years. The topics of teaching ranged from bench work to the
undergraduate researchers, to the specifics of podocyte culture with
collaborators, and 2d gel electrophoretic technique. I ran podocyte work shops
that was attended by post-doctoral fellows and faculty alike.
c. My University Services include: Peer Review, Public Service, Extracurricular and Other
Activities, as follows:
• University Service 1: Peer Research Reviewing for Journals: I have been a
peer reviewer of research articles submitted for publication to Journals listed
below:
• American Journal of Physiology Renal Physiology (2007-till date)
• Translational Research (2008-till date)
• Pharmacology, Experimental Biology & Medicine (2008-till date).
• The Application of Clinical Genetics (2009-till date).
• The Journal of Diabetes and Its Complications (2010-till date).
• Lab Investigation (2010-till date).
• Journal of Endocrinology (2013-till date)
• University Service 2: Public Service
• Chairman, Biomedical Library Advisory Committee (BLAC), UC San
Diego I served as the chairman of the BLAC for two terms between 2011-12
and 2012-13.
• Member of the BLAC
I served as a member of the BLAC between 2008-9 to 2011-12 for 3 terms. A
member of the UC San Diego BLAC is expected to provide advice to librarian
on issues pertaining to library services and policies. Committee members also
serve as liaison between the library and the member group they represent.
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• Podocyte Culture Related Workshops: To benefit the fellow-researchers I
have conducted podocyte culture workshops, during which I run practical
sessions to show how to carry out the podocyte culture. I do this because,
despite the comprehensive nature of the protocol write up, certain nuances that
cannot be captured in print exist. Podocyte culture is a complicated and
cumbersome operation, not a straightforward task. Cultured podocyte is a
critical tool required to address many questions that are being asked and
addressed, regarding the role of podocytes in healthy kidney as well as in
kidney disease. Since I have many years of experience in podocyte culture,
and have developed expertise and ability to troubleshoot any aspect of
podocyte culture, I conduct these workshops to explain the scruples of
culturing these important kidney cells and also give a live demonstration of
the entire process. Scientists from both UC San Diego as well as outside
(Scripps Research Institute) UC San Diego have benefitted from these
workshops, some of the individuals that benefited from these workshops
include:
o Dr. Karen Sykes, Marilyn Farquhar Lab, Dept. of Cellular and
Molecular Medicine, UC San Diego
o Dr. Kuixing Zhang, Ph. D., Post-doctoral Fellow, Dan O’Connor
Lab, Nephrology Division, School of Medicine, UC San Diego
o Dr. Celine DerMardirossian, Ph.D., Assistant Professor,
Immunology Department, The Scripps Research Institute
• University Service 3: Extracurricular Activities.
• Participation in relevant international meetings:
o 7th International Podocyte Meeting, Toronto, Canada, June 2008.
o The 2 nd Indo-US workshop on Mitochondrial Research &
Medicine 2008; Life Sciences Center, Manipal University,
Manipal, India
o 9 th International Human Proteome Organization Meeting on
Proteomics; Toronto, Canada. September 2009
o The International Society of Nephrology (ISN) Nexus Symposium
Geneva, Switzerland, 2010
o 45 th Annual Meeting & Scientific Exposition of the American
Society of Nephrology, San Diego, November 2012
o 5 th Annual Meeting of Proteomics Society – India, Bangalore, Nov
2013.
• Gave a talk entitled “Urinary Exosome Paradigm of Kidney
Failure: Clinical Utility of Exosome Proteomics”
• Panelist for the discussion entitled “ Application of Mass-
Spectrometry in Medical Research”
• Friday noon VA-Neuropathology Journal clubs
• Tuesday afternoon renal grand rounds
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• University Service 4: Other Academic activities:
• 1: Laboratory Service for collaborators: Serum creatinine estimation by
High Performance Liquid Chromatography:
o I have set up the High Performance Liquid Chromatography
(HPLC) (Perkin Elmer Instruments) method of estimating the
Serum Creatinine levels as the surrogate marker of Kidney
Glomerular Filtration Rate (GFR) Determination.9 th International
Human Proteome Organization Meeting on Proteomics held in
Toronto, Canada. September 2009
o The HPLC method is capable of reliably and quickly measuring
the serum creatinine levels in mouse serum (15 minute run time); It
is robust and sensitive (can measure creatinine at as low as 0.05
mg/dl). This ia a service that I render to collaborators within the
university and outside the university across the country.
o Our group is arguably the best among peers for Serum creatinine
estimation by HPLC, and the results obtained from our HPLC
serum creatinine protocol correlate very well with the pathology of
various kidney disease conditions known to affect the GFR.
o Earns revenue for the University.
• 2: Helped move the lab from Philadelphia to San Diego. Set up the lab for
Dr. Kumar Sharma’s research group in room # 410 of Stein Clinical Research
Building from scratch, June 21, 2007 onwards.
o Managed the efforts of half a dozen personnel to put the lab
equipment in place in Room 410 of the Stein Clinical Research
Building.
o Put in due diligence for Dr. Kumar Sharma’s lab to hire new
personnel, an animal technician and a laboratory research scientist.
Led and mentored a team of Renal Fellows, Postdocs and
Undergraduates performing Translational Proteomics research on
human urine, animal tissue, cell culture and exosomes to develop
biomarker discovery platforms. Nephrology-Division and
Department of Medicine committee of appointments and
promotions identified “engine driving the establishment of the
Translational Research Lab at UC San Diego”.
o Actively developed new collaborations for this translational
research lab.
2005 – 2007 Instructor of Medicine; Department of Medicine, Division of
Nephrology, Thomas Jefferson University.
My role as a faculty of the school of Medicine at Thomas Jefferson University was focused on
Research and Teaching:
• Performed proteomic research both independently and in conjunction with external
collaborators.
• Presented seminars with regard to applications of proteomic research to study diabetic
nephropathy.
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• Provided general proteomics guidelines to biochemical investigators from across
different departments at Jefferson.
• Initiated independent research programs focusing on
o Differential expression of peptides and its role in diabetic nephropathy – involving
the fractionation of peptides into 3 broad groups, namely 5 – 10 kDa; 3 – 5 kDa and
≤ 3 kDa; and further fractionation of comparatively more complex peptide fractions
by Size Exclusion Chromatography and subsequent identification of peptides using
nano-spray ESI-LC/MS/MS. The fractionation protocol lead to the following:
o Development of a generic method for technically more feasible and cost-effective
storage / retrieval of human urinary protein over long duration
o Comparative proteomic studies of diabetic nephropathy human urine samples in an
effort to identify biomarkers and disease progression markers for diabetic
nephropathy. These studies employed 2D-electrophoresis including DIGE
technology, Proteome Partitioning & removal of 12 most abundant proteins
(immunoaffinity-subtraction proteomics) and protein identification by LC/MS/MS
techniques.
o
Comparative proteomic studies of db/db mouse model kidney protein samples before
and after pirfenidone treatment in an effort to identify biomarkers and disease
activity / progression markers for diabetic nephropathy. These studies have so far
employed LC/MS/MS techniques (specifically label-free mass spectral quantitative
methods).
• Mentoring Senior Nephrology Fellowship-trainees in proteomic and protein chemical
research techniques.
2002 – 2004 Post-doctoral Fellow; Department of Medicine, Nephrology Division,
Thomas Jefferson University.
• Single-handedly standardized and applied numerous proteomic and related protein
analytical techniques to address biomarker discovery questions in Diabetic Nephropathy
at three levels namely i) human bio-fluid, ii) animal models and iii) renal cell culture.
Cultures in
o 2-dimension – for the analysis of total membrane, cell-surface & cytosolic subproteomes
via sub-cellular fractionation
o 3-dimension – encapsulated renal cell studies to mimic the kidney 3D structure
The techniques to study all three model systems generally involved simple protein
purification, protein separation – both chromatographic and electrophoretic: including
differential in-gel (DIGE) analysis, high-throughput robotic processing, mass spectral
analysis of proteins and peptides, mass spectral characterization of protein posttranslational
modification (e.g. phosphorylation, glycosylation), characterization of
protein-protein interactions, and independent validation by immunofluorescence, western
immunoblotting and ELISA. In addition to the above, renal cell studies featured further
fractionation into cytosol and total membrane sub-proteomes
• Specialized in differential protein expression and biomarker discovery studies comparing
normal and altered biological samples.
o Employed 2D-electrophoresis and multidimensional LC/MS/MS techniques for
these purposes.
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o Developed a variation of the DeCyder software for analysis of (statistically
significant) up- & down-regulation of spots in Sypro-stained 2D-gels featuring
proteins from two different populations (human mesangial cell sub-proteomes in
normal & high glucose)
o Independent follow-up and validation of the proteomic data by western
immunoblotting, confocal microscopy and ELISA
• Investigated the growth factor-induced cytoskeletal rearrangements in HUVECs and the
IP 3 R-biology in mesangial cells by biochemical and protein chemical approaches.
• Investigated the role of Nox4 protein in mediating stimulation of reactive oxygen species
by high glucose in human mesangial cells
• Mentored Senior Nephrology Fellowship-trainees in proteomics research techniques
1998 – 2001 Post-doctoral Fellow; Molecular Biophysics Unit, Indian Institute of
Science,Bangalore, India
• Investigated the biophysical aspects of the role of central linker of Calmodulin in target
recognition by Isothermal Titration Calorimetry.
• Studied fatty acid synthase 2 in Plasmodium falciparum, as an anti-malarial
chemotherapeutic target.
• Studied lectin affinity chromatography applications in diagnosis of clinical conditions
• Investigated the characterization of glycolipid-bearing liposomes as delivery vehicles
1993 – 1997 CSIR-UGC (Govt. of India) Research Fellow, Neurochemistry
Laboratory,Christian Medical College Hospital, Vellore, India.
• Investigated monkey cerebellum & spinal cord neurotransmitter receptor phosphorylation
– Ph.D thesis,
• Investigated changes in rat brain endogenous protein phosphorylation as a marker for the
different stages in the developing rat brain
• Investigated the zinc- and heparin- binding glycoprotein kinases from human cerebellum
and their endogenous substrates
• Studied phosphorylation as a functional probe of a 25 kDa peripheral nerve glycoprotein
(a receptor to Mycobacterium leprae, the leprosy-causing organism) – an original
finding
TECHNICAL SKILLS
• Protein: Purification and Characterization (Structural and Enzymatic –
Immunofluorescence-confocal, Western Immunoblotting, Immunohistochemistry and
ELISA), Protein and Peptide Analysis via Mass Spectrometry (MALDI-ToF,
Electrospray-Ion-trap LC/MS, SELDI), Gel Electrophoresis (1-D, 2-D, Isoelectric
Focusing, DIGE), Column Chromatography (HPLC, FPLC, 2-D LC applied in Size
Exclusion, Hydrophobic Interaction, Ion Exchange and Affinity settings), Fluorescent
Scanning and Imaging, High-throughput Robotics, Steady-State and Transient (pre-
Steady-State) Fluorescence Kinetics, Fluorescence and UV/VIS Spectroscopy,
Isothermal Titration Calorimetry and Differential Scanning Calorimetry;
Glycoprotein Structural Characterization & Microheterogeneity Determination via
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Mass Spectrometry, Differential Lectin Analysis & Serial Lectin Affinity
Chromatography, Protein function analysis by Radio Isotope Pulse Chase & Uptake
physiology in the presence of functional inhibitors; Radiation Safety Certified.
• Human Samples (HIPAA-, ERB- Certified); Biofluid analysis: Urine-Serum- & Kidney
Tissue Proteomics; Proteome partitioning: abundant / non-abundant proteins; Tissue
analysis: Cerebellum / Kidney Protein kinases & Receptors; endogenous
phosphorylation
• Animal Handling; Organ-level isolation from Monkey, rat, mouse, cow under
anaesthesia for protein / RNA studies; Glomerular isolation from mouse & rat kidney;
Clinical parameters (blood & urine glucose, TGF β, serum creatinine)
• Molecular and Cellular; genomic DNA and viral RNA purification, Transformation,
PCR/RT-PCR, Cell culture/transfection, In vitro viral propagation, Cloning /
Mutagenesis, Protein expression / purification, BL-3 Lab Certified
HONORS AND AWARDS
2009 NCLAM Awardee – National Center of Leadership in Academic
Medicine 2009 class participant.
2001 Research Associateship – Council of Scientific & Industrial Research,
Govt. of India, India
1998 Research Associateship – Dept. of Science & Technology, Govt. of
India, India
1996 Senior Research Fellowship – Council of Scientific & Industrial
Research, Govt. of India, India
1993 Junior Research Fellowship – Council of Scientific & Industrial
Research, Govt. of India, India
1993 Lectureship CSIR-University Grants Commission National Joint Entrance
Test, New Delhi, India
1990 6 th Rank National Entrance Examination (MSc Biochem.) Bangalore
Univ., Bangalore, India.
1992 1 st rank (Topper): Fellow Scientist’s position, CFTRI, Mysore, India
1991 Merit Scholarship Award, 1991-92, Karnataka State Teacher's
Association, Bangalore, India
1985 Merit Scholarship Award, 1984-85, BEL High School, Bangalore, India
for securing highest (Topper) marks in 5 categories viz., Maths, General
Science, Social Studies, Grammar & Languages (Kannada & English)
ADDITIONAL TRAINING
• Practical Liquid Chromatography/Mass Spectrometry (LC/MS) Techniques;
American Chemical Society Short courses, NJ 2006. An advanced course covering the
theory and application of coupling HPLC and microLC to different versions of Mass
Spectrometry.
• Mass Spectrometry of Glycoproteins Complex Carbohydrate Research Center, Univ.
of Georgia, Athens, Georgia, 2006. An advanced course covering the Structural
Characterization of Glycoproteins and Glycopeptides by Mass Spectrometry.
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PROFESSIONAL ASSOCIATIONS
2007 – present Member, World Human Proteome Organization (HUPO)
2007 – present Member, US Human Proteome Organization (US-HUPO)
2002 – present American Society for Nephrology, USA– participating fellow.
2005 – present American Chemical Society, USA– member.
2005 – present American Society for Mass Spectrometry, USA– member.
2005 – present Member of The Association of Biomolecular Resource Facilities
1993 – 2002 Society for Biological Chemists, India – member.
PUBLICATIONS
1. Satish P. RamachandraRao, Corrilynn O. Hileman, Chanthel Kokoy-Mondragon,
Jennifer A. Horng, Roni Khoshaba, Anousone Bounkhoun, Danielle Labbato, Robert T.
Schooley, and Grace A. McComsey Tamm-Horsfall protein levels in HIV infected
subjects associate inversely with levels of serum creatinine and soluble tumor
necrosis factor receptor. (Submitted to Journal of AIDS, 2014)
2. Satish P. RamachandraRao, Michael Matthias, Chanthel-Kokoy Mondrogon, Eamon
Aghania, Casey Kong, Michelle Ishaya, Jennifer Horng, Avital Slavin, Roni Khoshaba,
Fabrizio, Antonella De Palma, Anna Maria Agresta, Robert K. Naviaux, Ravi Mehta,
Joseph Vinetz, Priti Talwar and Pierluigi Mauri. Urine Exosome Proteomic Analysis
Reveals Renal Tubule-based Host Response to Leptospirosis Infection in Rats.
(Submitted to PLOS One, 2014)
3. Satish P. RamachandraRao, Chanthel Kokoy Mondragon, Marcela Zhou, Kumud
Singh and Robert Naviaux. Interleukin-1 Receptor Associated Protein Like-1 activity in
the kidney: Its role and interactions in diabetes. (In Preparation to be submitted to AJP,
2014).
4. T. Morgan, DuPriest E., Johnson J., Choate M., Anderson R., Farmer M., Zhao D.,
Woods L., RamachandraRao S. Lindner J., and A. Frias. "Zonal renal blood flow is
mediated by renal histidine decarboxylase expression" (submitted to JCI, 2014).
5. Satish P. RamachandraRao, Pam R. Taub, Chanthel Kokoy-Mondragon, Roni
Khoshaba, Anousone Bounkhoun, Michelle Ishaya, Eamon Aghania, Avital Slavin,
Assael Madrigal, Erin Higginbotham, Majid Ghassemian, Landon Wilson, Helen Kim,
Stephen Barnes, Ravindra L. Mehta and Robert K. Naviaux. Acute Kidney Injury
Associated Alteration in Mitochondrial Function Revealed by Urine Exosome
Proteomic Studies in Coronary Artery Bypass Surgery Subjects (Submitted to J
Proteomics, 2014)
6. Satish P. RamachandraRao, Alyssa Hamlin, , Marcela Zhou, Sandy Ramos, Casey
Kong, Rachael Overcash, Chanthel K. Mondrogon, Kelly Kao, Rosanna Overholser,
Jennifer A. Horng, Roni Khoshaba, Anousone Bounkhoun, Edith Esquada, Michael
Donohue, Majid Ghassemian Maryam Tarsa, and Robert K. Naviaux. Differential
protein profiling of urinary exosomes in human pregnancy reveals a role for cellular
danger response elements in diabetes/obesity (Submitted to Am. J Ob Gyn, 2014)
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7. Bansaghi S, Golenar T, Madesh M, Csordas G, RamachandraRao S, Sharma K, Yule
DI, Joseph SK, Hajnoczky G. Isoform-and species-specific control of IP3 receptors by
reactive oxygen species. J Biol Chem. 2014 Jan 27. [Epub ahead of print] PMID:
24469450
8. Østergaard J, Bjerre M, RamachandraRao S, Sharma K, Nyengaard JR, Hansen TK,
Thiel S, and Flyvbjerg A. Mannose-binding lectin in diabetic kidney disease: the impact
of mouse genetics in a type 1 diabetes model. Exp Diabetes Res. (2012). 2012: Article ID
678381, 9 pages.
9. Sharma K, Ix JH, Mathew AV, Cho M, Pflueger A, Dunn SR, Francos B, Sharma S,
Falkner B, McGowan TA, Donohue M, Ramachandrarao S, Xu R, Fervenza FC, Kopp
JB. Pirfenidone for Diabetic Nephropathy. J. Am. Soc. Neph. (2011). 22(6):11444-11451
10. Elizabeth Morse, Jana Schroth, Schinichi Okada, Satish RamachandraRao, Volker
Vallon, Kumar Sharma and Robyn Cunard “TRB3 expression is augmented in diabetic
kidneys and podocytes and reduces podocyte MCP-1 expression”. Submitted to Am J
Physiol. Renal Physiol. (2010) 5, F965-972
11. Gayen, Jiaur R., Zhang, Kuixing., RamachandraRao, Satish P., Mahata, Manjula.,
Chen, Yuqing., Naviaux, Robert K., Sharma, Kumar., Mahata Sushil K. and O’Connor,
Daniel T. “ Role of reactive oxygen species in hyperadrenergic hypertension:
Biochemical, physiological, and pharmacological evidence from targeted ablation of
the chromogranin A (Chga) gene”. Circulation: Cardiovasc Genet (2010) 3, 414-425
12. Yuexing Yuan, Xiangying Jiao, Wayne Bond Lau, Yajing Wang, Rong Li, Theodore A.
Christopher, Bernard L. Lopez, Satish P. RamachandraRao, Ling Tao, & Xin-Liang
Ma., “Glycative Inactivation of Thioredoxin-1 by Lipopolysaccharide: A Novel
Mechanism of Endotoxic Liver Injury”. (2010) Free Radical Biol Med..332-338
13. RamachandraRao, S.P., Shaw, M.A., and Sharma, K., “2-d Difference In-Gel
Electrophoresis (2D-DIGE) and Urinary Proteomics,” (2010) an invited review article for
“Renal and Urinary Proteomics: Methods and Protocols,” Wiley-VCH, publisher, V.
Thongboonkerd, editor.
14. Satish P RamachandraRao, Priti Talwar, Timothy Ravasi, and Kumar Sharma., “Novel
Systems Biology Insights Using Anti-Fibrotic Approaches for Diabetic Kidney Disease,”
(2010) Expert Rev. Endocrinol. Metab. 5(1), 127-135. Pub. by Future Science Group,
London, UK.
15. Satish P.RamachandraRao, Yanqing Zhu, Timothy Ravasi, Tracy A. McGowan, Irene
Toh, Stephen R. Dunn, Shinichi Okada, Michael A. Shaw and Kumar Sharma.,
“Pirfenidone is Renoprotective in Diabetic Kidney Disease” J. Am. Soc. Neph. (2009).
20(8):1765-75
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16. Kumar Sharma, Satish RamachandraRao, Gang Qiu, Hitomi Kataoka Usui, Yanqing
Zhu, Stephen R. Dunn, Raogo Ouedraogo, Kelly Hough, Peter McCue, Lawrence Chan,
Bonita Falkner, Barry J. Goldstein., (2008) “Adiponectin regulates albuminuria and
podocyte function in mice” J. Clin. Invest. 118(5): 1645-56
17. Satish P. RamachandraRao, Richard Wassell, M. Alexander Shaw & Kumar Sharma.
(2007) Expression Profiling of Human Mesangial Cell Sub-Proteomes Reveals a Role for
Calmodulin in Glucose Uptake; Am J Physiol. Renal Physiol; 292:1182-1189.
18. Ling Tao, Xiangyin Jiao, Erhe Gao, Wayne Lau, Yuexing Yuan, Bernard Lopez,
Theodore Christopher, Satish RamachandraRao, William Williams, Garry Southan,
Kumar Sharma, Walter Koch, Xin L. Ma, (2006). Nitrative Inactivation of Thioredoxin-1
and Its Role in Post-Ischemic Myocardial Apoptosis. Circulation. 114:1395-1402
19. Madesh M, Hawkins BJ, Milovanova T, Bhanumathy CD, Joseph SK, Ramachandrarao
SP, Sharma K, Kurosaki T, Fisher AB. (2005). Selective role for superoxide in InsP3
receptor-mediated mitochondrial dysfunction and endothelial apoptosis. J Cell
Biol.170:1079-1090.
20. Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein
BJ, Woolkalis M, Sharma K. (2005); Reactive oxygen species production via NADPH
oxidase mediates TGF-β-induced cytoskeletal alterations in endothelial cells. Am J
Physiol Renal Physiol; 289:F816-F825.
21. Sharma K, Lee S, Han S, Lee S, Francos B, McCue P, Wassell R, Shaw MA,
RamachandraRao SP. (2005). Two-dimensional fluorescence difference gel
electrophoresis analysis of the urine proteome in human diabetic nephropathy.
Proteomics. 5, 2648-2655.
22. Satish P. RamachandraRao, Avadhesha Surolia, Namita Surolia (2003) "Triclosan: A
Shot in the Arm for Antimalarial Chemotherapy": Molecular and Cellular Biochemistry
253: 55–63.
23. Posettihalli R. Satish & A.Surolia (2003). Sugars as Affinity Ligands: Chapter for the book
entitled “Methods for Affinity Based Separations of Enzymes & Proteins” in the series
Methods & Tools in Biosciences and Medicine. pp 115-128 Ed. M.N.Gupta.
24. Surolia N, Satish P. RamachandraRao, Surolia A. (2002) Paradigm shifts in malaria
parasite biochemistry and anti-malarial chemotherapy. Bioessays. 24,192-196.
25. Satish P.R., Surolia A. (2002) Preparation and characterization of glycolipid-bearing
multilamellar and unilamellar liposomes. Methods Mol Biol. 199, 193-202.
26. Satish P.R., Surolia A. (2001) Exploiting lectin affinity chromatography in clinical
diagnosis. J Biochem Biophys Methods. 49, 625-640.
Page 11 of 17

27. Anu K.Moorthy, B.Gopal, P.R.Satish, Sudha Bhattacharya, Alok Bhattacharya,
M.R.N.Murthy, A.Surolia. (1999) Thermodynamics of target peptide recognition by
calmodulin and a calmodulin analogue: Implications for the role of the central linker. FEBS
Lett. 461 19-24.
28. Suneetha, L.M., Satish, P.R., Korula, R.J., Suneetha, S.K., Job, C.K. and Balasubramanian,
A.S. (1998) Mycobacterium leprae binds to a 25 -kDa phosphorylated glycoprotein of
human peripheral nerve. Neurochem. Res. 23, 909-913.
29. Lavanya M. Suneetha, Posettihalli R. Satish, Sujai Suneetha, Charles K. Job and Aiylam S.
Balasubramanian. (1997) Mycobacterium leprae binds to a 28-30 – kDa phosphorylated
glycoprotein of rat peripheral nerve. Int. J. Leprosy 65, 352-356.
30. Satish P.R.and Balasubramanian, A.S. (1995) The neurotransmitter receptor triad: nicotinic
acetylcholine receptor, gamma-aminobutyric acid receptor (type A) and inhibitory glycine
receptor. Curr. Sci. 69, 336-342.
INVITED SCIENTIFIC PRESENTATIONS
1. Satish P. RamachandraRao. 4 th HKUPP workshop in HUPO2009 Annual Congress
The Westin Harbour Castle, Tornto, Canada September 26, 2009; Invited Talk on
“Proteomics to Interactomics: a streamlined approach to identify high-yield hits”
2. Satish P. RamachandraRao. National Center for Leadership in Academic Medicine
(NCLAM) 2009 graduation ceremony presentation, University of California, San Diego,
September 11, 2009; Talk delivered on “Mitochondria and DNA methylation in
Kidney Development”
3. Satish P. RamachandraRao. Nephrology Division Translational Medicine Research
Seminar Series, University of California, San Diego, March 18, 2009; Talk delivered
on “Podocyte Regeneration / recruitment”
4. Satish P. RamachandraRao. Juvenile Diabetes Research Foundation Site Visit talk held
at The Scripps Research Institute, San Diego, February 18, 2009; Talk delivered on
“Mitochondrial DNA and exosomal biomarkers of Diabetic Nephropathy”
5. Satish P. RamachandraRao. Department of Pathology, University of California, San
Diego, February 6, 2009; Talk delivered on “Podocyte Injury in the context of
Myosin 1e Disruption”
6. Satish P. RamachandraRao. 2 nd Indo-US meeting on Mitochondrial Research and
Medicine, sponsored by Indo-US Science & Technology Forum, held at the Manipal Life
Sciences Center, Manipal University, Manipal, India November 12-14, 2008; Invited
talk entitled “Mitochondrial DNA is reduced in the exosomes derived from podocytes
in the presence of high glucose and TGF-β.”
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7. Satish P. RamachandraRao. Department of Nephrology, JSS Medical College &
Hospital, Mysore, India August 19, 2008; Invited talk on “Proteomics:Uses and
Limitations in Biomarker Discovery”
8. Satish P. RamachandraRao. Department of Nephrology, Christian Medical College &
Hospital, Vellore, India, August 12, 2008; Invited talk on “Biomarker Discovery in
Kidney Disease”
9. Satish P. RamachandraRao. Department of Pathology, University of California, San
Diego, July 7, 2008; Talk delivered on “Podocytes as the Direct Target of
Adiponectin, a Kidney Disease Biomarker”
10. Satish P. RamachandraRao. Department of Pathology, University of California, San
Diego, June 20, 2008; Talk delivered on “Exosome Biomarkers in Human Health &
Disease”
11. Satish P. RamachandraRao. Nephrology Division Translational Medicine Research
Seminar Series, University of California, San Diego, Feb 4, 2008; Talk delivered on
“Metabolomic Tools in Human Diabetic Nephropathy”
12. Satish P. RamachandraRao & Kumar Sharma. Mid Atlantic Nephrology Young
Investigators Forum 2006 meet held at Pier 5 Hotel, Baltimore, MD. Talk delivered
(March 15-16) on “Expression Profiling of Podocytes under Hypoxia and in High
Ambient Glucose by Sypro Imaging – A Preliminary Screen for Selecting Candidate
Proteins”
13. Satish P. RamachandraRao & Kumar Sharma. 8 th EASD / JDRF Oxford Workshop
on ‘Prediction and Early Detection of Diabetic Complications: Common Pathways
and Unique Needs’ (convened jointly by European Association for Studies in Diabetes
& Juvenile Diabetes Research Foundation International) held at Keble College, Oxford
University, Oxford, Great Britain, between July 31-Aug 4, 2003 – as a speaker; delivered
a lecture on “The proteomic approach to developing insights in pathogenesis of
diabetic kidney disease”
14. Satish, P.R. and Balasubramanian, A.S. 1 st International Symposium on Frontiers in
Biology and Biotechnology, Christian Medical College & Hospital, Vellore, Tamilnadu
632 004, India. January 6-8, 1997. Oral presentation on "The Purification of
Neurotransmitter Receptors from the Monkey Brain"
15. Satish, P.R. Inter-University Lecture Series of Chemical Society of India, Bangalore
University Chapter. January 1992, Central College, Bangalore Universtity, India.
Lecture on “Applications of Nuclear Magnetic Resonance in Human Radio-
Diagnostics”
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ABSTRACTS / POSTERS
1. Satish P. RamachandraRao, Priti Talwar, Timothy Ravasi & Kumar Sharma: “Systems
Biology Approach to Understanding Mechanism of Action of Pirfenidone” 42 nd Annual
Meeting & Scientific Exposition of the American Society of Nephrology San Diego, CA,
November 2009.
2. Satish P. RamachandraRao & Kumar Sharma Poster on “Proteomics to
Interactomics: a streamlined approach to identify high-yield hits” HUPO2009
Annual Congress meeting, Tornto, Canada September 2009
3. Omoyemwen Igbinedion & Satish P. RamachandraRao, “Urinary Exosome Proteins as
Biomarkers for Renal Diseases” Genentech sponsored poster presentation at SACNAS
meeting, Salt Lake City, Utah, October 2008. The poster presented by Omo was
selected for an oral presentation, and won the best presentation award.
4. Satish P. RamachandraRao & Kumar Sharma “Adiponectin regulates oxidant stress in
Podocytes” 7th International Podocyte Meeting, Toronto, Canada, June 2008.
5. Satish P. RamachandraRao, Gang Qiu, Yanqing Zhu, Hitomi Kataoka Usui, Stephen R.
Dunn, Barry J. Goldstein & Kumar Sharma “Podocytes Are Target Cells for Adiponectin
Action” 40th Annual Meeting & Scientific Exposition of the American Society of
Nephrology San Fransisco, CA, November, 2007.
6. Satish P. RamachandraRao, M. Alexander Shaw, & Kumar Sharma; “Mouse Podocyte
Proteomes in High Glucose & Hypoxia” 39th Annual Meeting & Scientific Exposition of
the American Society of Nephrology San Diego, CA, November 15-18, 2006;
7. Chirag A. Patel, M. Alexander Shaw, Satish P. RamachandraRao & Satish Rattan;
“Proteomics of the Tonic Internal Anal Sphincter (IAS) Smooth Muscle of the Rat: A
Comparison with the Adjoining Phasic Rectal Smooth Muscle (RSM)” 3rd Annual
Meeting of the post doctoral researchers’ association, June 15-18, 2007;
8. Satish P. RamachandraRao, M. Alexander Shaw, & Kumar Sharma; “Nuclear Protein
Expression Profiling of db/db Mouse Kidney by Differential In-Gel Fluorescence
Electrophoresis (DIGE)” 38th Annual Meeting & Scientific Exposition of the American
Society of Nephrology Philadelphia, PA, November 8-13, 2005;
9. Daniel Lin, Bonita Falkner, M. Alexander Shaw, Satish P. RamachandraRao & Kumar
Sharma; “Urinary Proteome in African American Patients with Metabolic Syndrome”
38th Annual Meeting & Scientific Exposition of the American Society of Nephrology
Philadelphia, PA, November 8-13, 2005;
10. Kumar Sharma, SoHee Lee, Steven Han, Sung Lee, Barbara Francos, Peter McCue ,
Richard Wassell, M. Alexander Shaw, & Satish P RamachandraRao; “2D-DIGE
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Analysis of the Urine Proteome for Biomarker Discovery in Human Disease” BIO-2005
The Annual International Convention held at Philadelphia, PA, June 19-22, 2005;
11. Satish P RamachandraRao, Emilio D Ciccone, M Alexander Shaw, and Kumar
Sharma; “Sub-Proteomic Analysis of Human Mesangial Cell Reveals a Role for
Calmodulin in Glucose Uptake” 37th Annual Meeting & Scientific Exposition of the
American Society of Nephrology; St. Louis, Missouri, October 27-November 1, 2004
12. SoHee Lee, Sungchung Lee, Satish P. RamachandraRao, Barbara Francos, Stephen R.
Dunn, Tracy A.McGowan and Kumar Sharma.“Two dimensional Fluoresence
Differential Electrophoresis (DIGE) Analysis of the Urine Proteome in Human Diabetic
Nephropathy” 37th Annual Meeting & Scientific Exposition of the American Society of
Nephrology St. Louis, Missouri, October 27-November 1, 2004;
13. Kumar Sharma, Satish P. RamachandraRao, Yanqing Zhu, Emilio Ciccone, Cathryn
Valancius, Mahadev Kalyankar, & Barry Goldstein. “Stimulation of Reactive Oxygen
Species by High Glucose in Human mesangial Cells is Mediated via Nox4” 37th Annual
Meeting & Scientific Exposition of the American Society of Nephrology St. Louis,
Missouri, October 27-November 1, 2004;
14. Kumar Sharma, S.H.Lee, S.Lee, B.Francos, S.Dunn, & Satish P. RamachandraRao.
“Two Dimensional Fluorescence Difference Gel Electrophoresis (DIGE) Analysis of
Human Urine Proteome: use in Analysis of Human Diabetic Nephropathy” 6th Siena
Meeting: From Genome to Proteome: Biomarker Discovery & Imaging Proteomes,
Auditorium Giurisprudenza e Scienze Politiche, Via Mattioli 10, Siena, Italy. August 30
– September 2, 2004;
15. Satish P. RamachandraRao, and Kumar Sharma. “Human Mesangial Cell Subproteomes
in Diabetic Nephropathy” 36 th Annual Meeting & Scientific Exposition, San
Diego, CA, USA., November 12-17, 2003.
16. Satish P. RamachandraRao, Irene Toh, Taishan Hu, and Kumar Sharma. “Reactive
Oxygen Species as a Mediator of TGF-β1-induced Cytoskeletal Abnormalities in
Endothelial Cells” 36 th Annual Meeting & Scientific Exposition, San Diego, CA, USA.
November 12-17, 2003.
17. Satish, P.R. and Balasubramanian, A.S. "Modulation of a Human Cerebellum
Membrane-bound Glycoprotein Kinase Activity at the Substrate Level" 5 th International
Symposium on Biochemical Roles of Eukaryotic Cell Surface Macromolecules,
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India, January 4-8,
1999.
18. Satish, P.R. and Balasubramanian, A.S. "Protein Phosphorylation and the
Neurotransmitter Receptor Triad" 66 th Annual Meeting of the Society for Biological
Chemists, India. Biochemistry Department, Andhra University, Vishakapatnam, India.
December 22-24, 1997.
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ORGANIZING ROLES IN SCIENTIFIC MEETINGS:
• Organized the local hospitality and transportation of participating members during the 1 st
International Symposium on Frontiers in Biology & Biotechnology, held at Christial
Medical College & Hospital, Vellore, India (Jan 6-8, 1997).
• Organized the travel of participating glycobiologists to spots of tourist interest in and
around Bangalore, India, during the 5 th International Symposium on Biochemical Roles
of Eukaryotic Cell Surface Macromolecules held at the Molecular Biophysics Unit,
Indian Institute of Science, Bangalore, India (January 4-8, 1999).
COMMUNITY SERVICE:
2013: Panel discussion in the 5 th Annual Meeting of Proteomics Society-
India to promote the awareness of the latest developments in the field
of proteomics among clinicians, final MBBS and PG students. Topic
was entitled “Application of Mass Spectrometry in Medical Research”
2013: Served as one of the judges to determine the competence of the posters
presented for the best poster award during the 5 th Annual Meeting of
Proteomics Society-India
2013: Served as a judge to determine the competence of young children in
High School for Academic Learning on Congressional Debates,
Oratorical Interpretations and Impromtu Speech contests.
2008-Present Teaching Histology as part of pharmacology course to a class of 65
students at the Skagg’s School of Pharmacy & Pharmacology
2008-Present
2007-Present
2005-Present
Talking to Elementary School Children (3 rd to 5 th grade) on ‘how the
Kidney functions’ ‘Importance of drinking water’, ‘What is type 1 and
type 2 diabetes’ ‘why do kidneys fail’ and such topics to help
inculcate critical thinking and an interest towards science among
school-going children.
Running workshops on how to culture podocytes to various assistant
professors, post-docs, and technicians in the Dept of Medicine and
around UC San Diego and The Scripps Research Institute.
Reviewer of articles for various journals (American Journal of
Physiology (Renal Physiology), Pharmacology, Experimental Biology
& Medicine, etc).
1990 – 1993 Teaching Biochemistry and Chemistry to High School and 10+2 level
students,
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1990 – 1993 Coaching +2 level students for the State Level Common Entrance Test
(in Chemistry & Biology) for Professional Courses viz., Medicine &
Engineering
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