B. Tech Biology-Elective-2 Semester-6 Industrial Microbiology - rgukt
B. Tech Biology-Elective-2 Semester-6 Industrial Microbiology - rgukt
B. Tech Biology-Elective-2 Semester-6 Industrial Microbiology - rgukt
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Paper code: BIMB 602<br />
B. <strong>Tech</strong><br />
<strong>Biology</strong>-<strong>Elective</strong>-2<br />
<strong>Semester</strong>-6<br />
<strong>Industrial</strong> <strong>Microbiology</strong>: Paper-I<br />
(Fundamentals, Down stream processing, Enzyme <strong>Tech</strong>nology)<br />
Unit 1: Fundamentals of industrial microbiology<br />
1. Introduction to industrial microbiology<br />
2. Classification of microbes –systems of classification, numerical taxonomy,<br />
identifying characters for classification<br />
3. Ultrastructure of bacteria in general, important differences between<br />
archae bacteria and bacteria, features of special interest of cynobacteria.<br />
4. Screening for industrially important Microbs<br />
5. Various methods of staining of bacteria –simple ,gram, endospore, capsule,<br />
flagella.<br />
6. Viruses –general account of viruses –plant viruses and animal viruses, ultra<br />
structure of viruses.<br />
7. Nutritional requirements and nutritional types of bacteria, choice of media<br />
and conditions of incubation.<br />
8. Pure culture techniques, preparation of different types of culture media ,<br />
selective or enrichment media . Methods of culturing aerobic and<br />
anaerobic bacteria<br />
9. Concepts of sterilization, definition of sterilization, dry and moist heat,<br />
pasteurization, tyndalization, radiation, ultrasonication, filtration.<br />
10. Methods of sterilization used in industrial microbiology –physical and<br />
chemical, disinfection sanitization, antisepsis sterilants and fumigation.<br />
11. Microbial growth –growth curve, mathematical expression of growth,<br />
methods of measurements of growth, factors affecting growth in<br />
continuous and batch cultures.<br />
12. Strain developoment –methods of improvement and stability of<br />
biotechnologically important cultures.<br />
13. Control of microorganisms by physical and chemical agents , pattern of<br />
microbial death , factors affecting effectiveness of antimicrobial agents<br />
activity.<br />
Unit-2 .Fermentor design<br />
14. Types of fermentation, aerobic, anaerobic, batch, continuous, submerged,<br />
surface, solid state
15. Design of typical fermentor<br />
16. Types of fermentors<br />
17. Control of agitation , aeration, pH, temperature and dissolved oxygen<br />
18. Inoculum development - - scale up of fermentation process.<br />
Unit-3 Down stream processing<br />
19. Separation of particles- disintegration of cells, extraction<br />
20. Concentration recovery and purification-drying<br />
21. In-situ recovery of products –application<br />
Unit-4: Enzyme <strong>Tech</strong>nology<br />
22. Nature of enzyme –application of enzymes<br />
23. Limitations of microbial cells used as catalysts in fermentation –multi<br />
enzyme reactors<br />
24. Genetic engineering and protein engineering of enzymes<br />
25. Cloning strategy for enzymes<br />
26. <strong>Tech</strong>nology of enzyme production<br />
27. Methods of immobilized cells and enzymes ( ca-alginate beads,<br />
Polyacrylamide)<br />
28. <strong>Industrial</strong> application of immobilized enzymes and cells.<br />
Unit-5: Stains and staining <strong>Tech</strong>iques<br />
29. Definition of auxochrome –chromophores. Dyes –classification of stains<br />
30. Theories of staining. Mechanism of gram staining ,acid fast staining<br />
,negative staining ,capsule staining flagella staining , endospore staining<br />
<strong>Industrial</strong> microbiology<br />
Paper-1(practicals)<br />
1. Safety measures in microbiology laboratory, cleaning and sterilization of<br />
glassware .<br />
2. Study of instruments; compound microscope, autoclave , hot air oven, pH<br />
meter, laminar airflow and centrifuge.<br />
3. Staining techniques –simple ,negative staining ,gram staining , endospore<br />
staining and fungal staining<br />
4. Media preparation- nutrients agar, MRBA and nutrient broth.<br />
5. Isolation and screening for amylase enzyme.<br />
6. Screening for antibiotic.<br />
7. Recovery of Citric acid.<br />
8. Estimation of microorganisms- total count (Haemocytometer).<br />
9. Motility by hanging drop method<br />
10. Use of counting chamber for bacterial count
11. Effect of temperature and pH on bacterial growth<br />
12. Immobilization of amylase by using sodium alginate method<br />
Scheme of practical examination<br />
(<strong>Semester</strong>-6)<br />
1. Isolation of micro organisms by sector plate method 12<br />
2. Differential staining 10<br />
3. Special staining 10<br />
4. Spotting 8<br />
5. Viva voice 5<br />
6. Practical record 5<br />
Total 50marks<br />
Books<br />
1. A text book of basic and applied microbiology –Aneja. K.R etal.<br />
2. General microbiology – Power,C.B & Daginewala<br />
3. <strong>Industrial</strong> microbrology –patel. A.H<br />
4. <strong>Microbiology</strong> –pelezer, m.J, chan, E.C.S & krieg. N.R<br />
5. <strong>Microbiology</strong> –Tauro, yadav& kapoor<br />
6. <strong>Microbiology</strong> – Tortora,G.J, Funke, B.R, case, c.c<br />
7. <strong>Biology</strong> of microorganisms- Brock and madigan<br />
8. Introduction to microbiology – Ingraham and Ingraham<br />
9. Elementary microbiology – H.A. Modi<br />
10. Text book of microbiology – Dubey and maheshwari<br />
11. General microbiology -stainer<br />
12. <strong>Industrial</strong> microbiology – Prescott and Dunn<br />
13. <strong>Microbiology</strong> – purohit<br />
14. Principles of fermentation technology – stanbury , P.F, Whitaker, A and<br />
Hall. S.J<br />
15. <strong>Industrial</strong> microbiology –cassida
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