Molecular Biology, Biochemistry and Genetics - rgukt

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2 5.2 P properties of DNA ( UV-Absorption spectra, Denaturation and Renaturation, Cot Curves, DNA hybridization) 5.3 DNA Replication – General features of DNA Replication, Enzymes and proteins of DNA replication. 5.4 Models of replication, Prokaryotic and Eukaryotic replication mechanism. 5.5 DNA damage & DNA repair – causes and mechanism – photo reactivation, excision repair, mismatch repair, SOS repair 5.6 DNA Recombination – Homologous recombination – holiday medal, Nonhomologous recombination- Transposition. 5.7 Types of RNA and their function. Outlines of RNA biosynthesis in Prokaryotes. 5.8 Structure of Prokaryotic and Eukaryotic gene 5.9 Genetic code, properties and wobble hypothesis 5.10 Transcription in prokaryotes -Mechanism, Promoters and RNA polymerase, transcription factors 5.11 Transcription in Eukaryotes 5.12 Post transcriptional modifications of eukaryotic mRNA, Reverse transcription. 5.13 Translation – Mechanism of translation in Prokaryotes and Eukaryotes, Post translational modifications of Proteins 5.14 Regulation of Gene activity in Prokaryotes – Operon concept- induction, Repression and Attenuation, Inducible Operon (lac operon) – Repressible Operon (tryp operon) 5.15 Regulation of Gene expression in Eukaryotes – transcriptional activation, galactose metabolism in yeast. 5.16 Gene organization and expression in mitochondria and Chloroplasts.
3 Unit-6 Genetics 6.1 Mendels laws of heredity with examples, Test cross and back cross, The principle of independent assortment 6.2 Interaction of genes – Incomplete dominance, co-dominance, lethal genes, epistasis, Pleiotropy, 6.3 Polygenic traits and quantitative inheritance 6.4 Genomic organization in Prokaryotes 6.5 Genomic organization in Eukaryotes 6.6 Mutations – Gene and chromosomal mutations, Natural and induced mutations – mutagenic agents 6.7 Molecular basis of gene mutations, Role of mutations in evolution. 6.8 Gene concept – classical concept –Molecular concept of gene- transposons, Pseudogenes, overlapping gene, oncogene, repeated gene, Gene amplification. 6.9 Fine structure of gene 6.10 Genetic Recombination-1: Gene transfer in bacteria – Transformation- Transposable elements-Plasmid structure, Properties and types of Plasmids. 6.11 Genetic Recombination-II: Transduction – U tube experiment – Generalized and specialized transduction. Conjugation and steps in conjugation. 6.12 Human Genetic disorders – (Klinefelter syndrome and Turner’s syndrome). 6.13 Population Genetics: Population, Gene pool, mutation and selection, Hardy- Weinberg law and its significance.
Page 1: 1 B. Tech-Biology-Minor subject II
Page 5: 5 Genetics 4. Fundamentals of Bio c

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