Molecular Biology, Biochemistry and Genetics - rgukt

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B. Tech-Biology-Minor subject
II nd Year-Semester-II
Core course (paper – II )
(Course Code-B 1 2002)
(Molecular Biology , Biochemistry and Genetics)
Unit – 4: Bio chemistry
4.1 Carbohydrates - Classification, physical and chemical properties, chemical
structures and their importance.
4.2 Amino acids - Classification and properties – Zwitterion nature
4.3 Proteins - Classification, structure and function, primary, secondary, tertiary
and quaternary structure
4.4 Enzymes - Classification, General Characteristics, mechanism of enzyme
action, co enzymes, co factor, isozymes
4.5 Regulation of enzyme activity and factors affecting enzyme activity,
competitive, un competitive and non competitive inhibition.
4.6 Lipids – structure, classification, properties and functions of lipids, saturated
and unsaturated fatty acids.
4.7 Bioenergetics – Principles of bioenergetics and high energy phosphate
compounds, mode of energy production-photo phosphorylation
Unit- 5 : Molecular Biology
5.1 PhysicalDNA Structure – Chemistry of DNA, Forces stabilizing DNA Structure,
Helix parameters. Forms of DNA (A,B,C,D,T and Z)

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5.2 P properties of DNA ( UV-Absorption spectra, Denaturation and Renaturation,
Cot Curves, DNA hybridization)
5.3 DNA Replication – General features of DNA Replication, Enzymes and
proteins of DNA replication.
5.4 Models of replication, Prokaryotic and Eukaryotic replication mechanism.
5.5 DNA damage & DNA repair – causes and mechanism – photo reactivation,
excision repair, mismatch repair, SOS repair
5.6 DNA Recombination – Homologous recombination – holiday medal, Nonhomologous
recombination- Transposition.
5.7 Types of RNA and their function. Outlines of RNA biosynthesis in Prokaryotes.
5.8 Structure of Prokaryotic and Eukaryotic gene
5.9 Genetic code, properties and wobble hypothesis
5.10 Transcription in prokaryotes -Mechanism, Promoters and RNA
polymerase, transcription factors
5.11 Transcription in Eukaryotes
5.12 Post transcriptional modifications of eukaryotic mRNA, Reverse
transcription.
5.13 Translation – Mechanism of translation in Prokaryotes and Eukaryotes, Post
translational modifications of Proteins
5.14 Regulation of Gene activity in Prokaryotes – Operon concept- induction,
Repression and Attenuation, Inducible Operon (lac operon) – Repressible
Operon (tryp operon)
5.15 Regulation of Gene expression in Eukaryotes – transcriptional activation,
galactose metabolism in yeast.
5.16 Gene organization and expression in mitochondria and Chloroplasts.

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Unit-6 Genetics
6.1 Mendels laws of heredity with examples, Test cross and back cross, The
principle of independent assortment
6.2 Interaction of genes – Incomplete dominance, co-dominance, lethal genes,
epistasis, Pleiotropy,
6.3 Polygenic traits and quantitative inheritance
6.4 Genomic organization in Prokaryotes
6.5 Genomic organization in Eukaryotes
6.6 Mutations – Gene and chromosomal mutations, Natural and induced
mutations – mutagenic agents
6.7 Molecular basis of gene mutations, Role of mutations in evolution.
6.8 Gene concept – classical concept –Molecular concept of gene- transposons,
Pseudogenes, overlapping gene, oncogene, repeated gene, Gene
amplification.
6.9 Fine structure of gene
6.10 Genetic Recombination-1: Gene transfer in bacteria – Transformation-
Transposable elements-Plasmid structure, Properties and types of Plasmids.
6.11 Genetic Recombination-II: Transduction – U tube experiment – Generalized
and specialized transduction. Conjugation and steps in conjugation.
6.12 Human Genetic disorders – (Klinefelter syndrome and Turner’s
syndrome).
6.13 Population Genetics: Population, Gene pool, mutation and selection, Hardy-
Weinberg law and its significance.

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B.Tech-Minor subject-Biology
Practical-Paper-II (Lab-I)
II nd year –II nd semester
1. Defferent stages of Mitosis in onion root tip
2. Permanent slides- polytene chromosomes, grass hopper spermatids and
chromosomes
3. Effect of temperature on enzyme activity(Amylase)
4. Quantitative estimation of Proteins by Lowry’s method
5. Quantitative estimation of carbohydrates by Nelson Smogyi method
6. Estimation of DNA by UV spectrophotometry
7. Electrophoretic analysis of DNA
8. Preparation of solid and liquid culture media and their sterilizationculturing
techniques (Bacteria)
9. Isolation of pure culture by Streak plate and Pour plate method
10. Growth of bacteria on agar slant, agar stab, petriplate and in broth- Growth
curves
11. Staining techniques- simple staining, Gram staining, negative staining
Books
Bio Chemistry
1. Principles of Bio chemistry – A. Lehninger, D.L Nelson worth publishes
2. Principles of Bio chemistry – G.L. Zubay, W.W. Parson- Brown Publisher.
3. Bio chemistry – V edition – Jeremy M.Berg, Free man &Co.

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Genetics
4. Fundamentals of Bio chemistry – Dr. A.C. Deb
5. Fundamentals of Bio chemistry – J.L. Jain,Sunjay Jain and Nitin Jain
S.Chand &Co.
6. Harpies Bio chemistry, murray RK etal – Prentice Hall International
7. Bio chemistry – Stryer L, H – Freeman & Co
8. Text book of Biochemistry – Davlin, thomas-M.
9. Bio chemistry Fundamentals, voet etal
10. Bio chemistry – Fried fider .D
11. Practical Bio chemistry – Plummer.
12. Outlines of Biochemistry – conn and stump. Wiley Eastern ltd.
1. Principles of Genetics – E. L Gardner, M.J. Simmons – John wiley & sons
publication
2. Genetics – Strick burger M.W – Macmillan publisher
3. Human Molecular Genetics – Strachan T and Read AP-harland science..
4. Microbial Genetics – Maloy etal
5. Concepts of Genetics 7 th edition (2003) – Klug & Cumming
6. Microbial Genetics – Frid fleder
7. Advanced Genetics (2002) – Miglani, G-S
8. Fundamentals of Genetics – B.D. Singh.
9. Genetics –Arora and Sandhu
10. Genetics –P.K.Gupta