GBM annual fall meeting - Wissenschaft Online

Chenna Reddy Galiveti, Steffen Hennig, James Adjaye, Ralf Sudbrak, Michal Janitz,
Hans Lehrach, Jürgen Brosius, Zoltán Konthur
RNomics – Analysis of novel in silico derived non-protein
coding RNAs in the human genome
Since the increased complexity of vertebrates over invertebrates (e.g. human versus
C.elegans with 10 14 and 10 4 cells, respectively) cannot be explained by a mere 1.5 fold
increase in protein-coding genes, it becomes inevitable that genomic sequences resulting
in non-protein coding RNA molecules (npcRNAs) might play a much more substantial role
in gene regulation networks, than expected. Increasing evidence shows that most of the
complex genetic phenomena in higher organisms, such as gene silencing, imprinting,
targeting, splicing, RNA editing and methylation are connected to RNA signalling and the
involvement of different classes of npcRNAs is the rule rather than the exception. The
rapidly growing list of functional npcRNAs shows great diversity and suggests that by
now we have only seen a small fraction of all functional npcRNAs.
Within an Exploratory Project of the German National Genome Research Network
(NGFN), we have set out to investigate the possibility to identify novel npcRNAs encoded
in the human genome by a computational approach followed by the evaluation through a
set of experimental methods including RT-PCR, real-time PCR and northern
hybridisation. At a later stage, the novel npcRNAs will be assessed for expression levels
in different tissues and screened for potential interaction partners in active RNP
complexes through gel retardation assays, phage-displayed cDNA expression product
library screening, mass spectroscopy and transfected cell arrays.
contact:
Mr. Chenna Reddy Galiveti
Max Planck Institute for Molecular Genetics
Department of Vertebrate Genomics
galiveti@molgen.mpg.de
Ihnestr. 73
14195 Berlin (Germany)
GBM annual fall meeting
Berlin/Potsdam
September 18-21, 2005