Mycoplasma - Minerva Biolabs
Mycoplasma - Minerva Biolabs
Mycoplasma - Minerva Biolabs
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Products for the control of microbials in cell cultures & water.<br />
<strong>Mycoplasma</strong> Off<br />
<strong>Mycoplasma</strong>-Off is a highly effective solution for cleansing and disinfection of all suitable laboratory surfaces and apparatus, including clean<br />
benches, incubators, work benches, cell storage boxes and liquid nitrogen containers from mycoplasma contamination.<br />
<strong>Mycoplasma</strong>-Off is an alcohol-based solution of the powerful mycoplasma elimination reagent Mynox. It can be used as a routine contamination<br />
control program or for known or suspected mycoplasma challenges.<br />
<strong>Mycoplasma</strong>-Off is outstandingly safe in use possessing not only a strong mycoplasmacidal effect, but also being effective against bacteria, yeasts<br />
and viruses: <strong>Mycoplasma</strong>-Off has been proven to be effective against bacteria like Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa,<br />
Staphylococcus aureus, Enterococcus hirae, Trichopyhton mentagrophytes, against viruses like vaccinia viridae, BVD, polio, adeno, polyoma SV40 and<br />
MNV (norovirus) and Candida albicans, according to the requirements of CEN TC 216.<br />
The effectiveness of <strong>Mycoplasma</strong>-Off is further enhanced by its excellent detergent properties, so that clean contamination-free surfaces can be<br />
achieved in a single application. <strong>Mycoplasma</strong>-Off is a ready-to-use solution supplied in a refillable spray bottle.<br />
Background<br />
It is speculated that more than 60-70 % of the cell cultures world wide are contaminated with mycoplasma. <strong>Mycoplasma</strong> are the smallest selfreplicating<br />
organisms (0.1 to 0.8 µm) known thus far. <strong>Mycoplasma</strong> lack a cell wall which makes them resistant to cell wall specific antibiotics, such<br />
as penicillin, but susceptible to heat and chemicals such as alcohols and aldehydes. <strong>Mycoplasma</strong> occur in many places in nature, and laboratory<br />
appliances such as incubators, providing ideal living conditions.<br />
The usual way of contamination is a media spill from a contaminated cell culture during loading and unloading of the cabinet. These spills can<br />
contaminate the trays and the water reservoir. <strong>Mycoplasma</strong> are known to survive a distinct time on smooth surfaces even after the spilled culture<br />
medium has dried out. More serious is a contamination of the water tank needed to control the moisture level. <strong>Mycoplasma</strong> can survive and, as some<br />
experts assume, also grow in pure water. The contamination with mycoplasma and other bacteria in water reservoirs can effectively be minimized by<br />
adding WaterShield as preventive.<br />
For mycoplasma contamination, a treatment with <strong>Mycoplasma</strong>-Off was found to be very effective. The water bath should be removed and the interior<br />
completely sprayed with <strong>Mycoplasma</strong>-Off. After incubation for 2-3 minutes the incubator should be wiped clean with paper towels and loaded with<br />
fresh water. Keep the incubator open for 10 minutes to allow evaporation of remaining <strong>Mycoplasma</strong>-Off.<br />
Suggestions for avoiding contamination with mycoplasma in cell culture incubators:<br />
• Frequent decontamination of the incubator by spraying with <strong>Mycoplasma</strong>-Off and changing of the water, at least every 14 days.<br />
• Add WaterShield to water reservoirs for prevention of microbiolal contamination.<br />
• Prevent spills by using culture flasks with filter caps, which can be tightly closed; when using petri dishes, small trays should be used to carry the<br />
plates in and out, prevent blocking the air flow in the chamber<br />
General suggestions:<br />
• Essential: routine testing on mycoplasma contamination of the cells and discarding positive tested cultures, test every new cell culture which<br />
comes to your lab.<br />
• If possible, separate your cultures into proven "mycoplasma negative" cultures and untested or "mycoplasma positive" cultures and grow them in<br />
separate incubators.<br />
• Always use glows while handling the cultures, spray your glows with <strong>Mycoplasma</strong>-Off frequently after each working step or before handling a<br />
new culture. <strong>Mycoplasma</strong> inhabit the human pharyngeal cavity as parasites. By coughing or sneezing mycoplasma are transferred to our hands.<br />
Recommended Use<br />
Applicable in research and industry only. Not recommended for clinical applications. Use as directed. Always test a small area first for stability and<br />
coloration. <strong>Mycoplasma</strong>-Off should not be used on alcohol sensitive surfaces like acrylic and fiber glass.<br />
Kit Components<br />
Ready-to-go formulation in a spray bottle or refill bottles.<br />
Shelf Life and Storage<br />
Stable at room temperature for at least 12 months.<br />
Composition<br />
32,5 % 1-propanol - 18 % ethanol - 0,1 % glutaraldehyde - 0,05 % Mynox®<br />
Package Sizes<br />
Cat.-No. 15-1000 1 litre<br />
Cat.-No. 15-5000 5 x 1 litre refill bottles<br />
Scan for additional<br />
web information about<br />
<strong>Mycoplasma</strong> Off<br />
Contact<br />
<strong>Minerva</strong> <strong>Biolabs</strong> GmbH / Koepenicker Str. 325 / 12555 Berlin / Germany<br />
Tel. +49 (0)30 2000437-0 / Fax +49 (0)30 2000437-9<br />
info@minerva-biolabs.com / www.minerva-biolabs.com<br />
EN ISO 9001<br />
EN ISO 13485<br />
Certified Management System<br />
© 2012 <strong>Minerva</strong> <strong>Biolabs</strong> GmbH / FL32.0EN
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