Mycoplasma - Minerva Biolabs

Mycoplasma
Off
Broad Spectrum of Antimicrobial Activity
Bactericidal, fungicidal, and virucidal! Mycoplasma-Off
is outstandingly safe in use.
Rapid
Mycoplasma-Off affects mycoplasma immediately.
Within seconds mycoplasma are completely eradicated.
Convenient and Ready-to-Use
Mycoplasma-Off is a ready-to-use solution supplied in
a refillable spray bottle. The use of Mycoplasma-Off
is fast, easy and ideal to maintain a clean work area.
Non-Corrosive and Non-Carcinogenic
Mycoplasma-Off is an alcohol-based, non-corrosive and
non-carcinogenic solution of the powerful mycoplasma
elimination reagent Mynox.
Products for the control of microbials in cell cultures & water.

Products for the control of microbials in cell cultures & water.
Mycoplasma Off
Mycoplasma-Off is a highly effective solution for cleansing and disinfection of all suitable laboratory surfaces and apparatus, including clean
benches, incubators, work benches, cell storage boxes and liquid nitrogen containers from mycoplasma contamination.
Mycoplasma-Off is an alcohol-based solution of the powerful mycoplasma elimination reagent Mynox. It can be used as a routine contamination
control program or for known or suspected mycoplasma challenges.
Mycoplasma-Off is outstandingly safe in use possessing not only a strong mycoplasmacidal effect, but also being effective against bacteria, yeasts
and viruses: Mycoplasma-Off has been proven to be effective against bacteria like Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa,
Staphylococcus aureus, Enterococcus hirae, Trichopyhton mentagrophytes, against viruses like vaccinia viridae, BVD, polio, adeno, polyoma SV40 and
MNV (norovirus) and Candida albicans, according to the requirements of CEN TC 216.
The effectiveness of Mycoplasma-Off is further enhanced by its excellent detergent properties, so that clean contamination-free surfaces can be
achieved in a single application. Mycoplasma-Off is a ready-to-use solution supplied in a refillable spray bottle.
Background
It is speculated that more than 60-70 % of the cell cultures world wide are contaminated with mycoplasma. Mycoplasma are the smallest selfreplicating
organisms (0.1 to 0.8 µm) known thus far. Mycoplasma lack a cell wall which makes them resistant to cell wall specific antibiotics, such
as penicillin, but susceptible to heat and chemicals such as alcohols and aldehydes. Mycoplasma occur in many places in nature, and laboratory
appliances such as incubators, providing ideal living conditions.
The usual way of contamination is a media spill from a contaminated cell culture during loading and unloading of the cabinet. These spills can
contaminate the trays and the water reservoir. Mycoplasma are known to survive a distinct time on smooth surfaces even after the spilled culture
medium has dried out. More serious is a contamination of the water tank needed to control the moisture level. Mycoplasma can survive and, as some
experts assume, also grow in pure water. The contamination with mycoplasma and other bacteria in water reservoirs can effectively be minimized by
adding WaterShield as preventive.
For mycoplasma contamination, a treatment with Mycoplasma-Off was found to be very effective. The water bath should be removed and the interior
completely sprayed with Mycoplasma-Off. After incubation for 2-3 minutes the incubator should be wiped clean with paper towels and loaded with
fresh water. Keep the incubator open for 10 minutes to allow evaporation of remaining Mycoplasma-Off.
Suggestions for avoiding contamination with mycoplasma in cell culture incubators:
• Frequent decontamination of the incubator by spraying with Mycoplasma-Off and changing of the water, at least every 14 days.
• Add WaterShield to water reservoirs for prevention of microbiolal contamination.
• Prevent spills by using culture flasks with filter caps, which can be tightly closed; when using petri dishes, small trays should be used to carry the
plates in and out, prevent blocking the air flow in the chamber
General suggestions:
• Essential: routine testing on mycoplasma contamination of the cells and discarding positive tested cultures, test every new cell culture which
comes to your lab.
• If possible, separate your cultures into proven "mycoplasma negative" cultures and untested or "mycoplasma positive" cultures and grow them in
separate incubators.
• Always use glows while handling the cultures, spray your glows with Mycoplasma-Off frequently after each working step or before handling a
new culture. Mycoplasma inhabit the human pharyngeal cavity as parasites. By coughing or sneezing mycoplasma are transferred to our hands.
Recommended Use
Applicable in research and industry only. Not recommended for clinical applications. Use as directed. Always test a small area first for stability and
coloration. Mycoplasma-Off should not be used on alcohol sensitive surfaces like acrylic and fiber glass.
Kit Components
Ready-to-go formulation in a spray bottle or refill bottles.
Shelf Life and Storage
Stable at room temperature for at least 12 months.
Composition
32,5 % 1-propanol - 18 % ethanol - 0,1 % glutaraldehyde - 0,05 % Mynox®
Package Sizes
Cat.-No. 15-1000 1 litre
Cat.-No. 15-5000 5 x 1 litre refill bottles
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Mycoplasma Off
Contact
Minerva Biolabs GmbH / Koepenicker Str. 325 / 12555 Berlin / Germany
Tel. +49 (0)30 2000437-0 / Fax +49 (0)30 2000437-9
info@minerva-biolabs.com / www.minerva-biolabs.com
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© 2012 Minerva Biolabs GmbH / FL32.0EN