large - NordiQC

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Immunohistochemistry: A highly complex analysis Decalcification Preparation Tissue Type, Dimension, De-differentiation Necrosis Heat cauterisation Control Quantification Reporting Fixation Time, Type, Volume Preanalytic Postanalytic Section Thickness Storage Drying 3 choices for 5 variables in each phase: 4 million different protocols ! Pre-treatment Manual Stainer Visualization Sensitivity, Specificity Primary antibody Clone, Dilution Buffer, Time, Temp Analytic Interpretation Localization Positive/Negative - cut-off level Development Sensitivity, Localization
External Quality Assurance: Estrogen Receptor Correct False negative ½ Control Lab. A Lab. B 3
Page 1: DAKO Satellite Symposium: CIQC/CAP-
Page 5 and 6: External Quality Assurance • Stai
Page 7 and 8: Website: www.nordiqc.org 7
Page 9 and 10: Over-all assessment results NordiQC
Page 11 and 12: Results of NordiQC tailored recomme
Page 13 and 14: External IHC Quality Assurance •A
Page 15 and 16: External Quality Assurance “Less
Page 17 and 18: Poor antibodies - CD31 false negati
Page 19 and 20: Poor antibodies - MLH1 false positi
Page 21 and 22: Less robust Abs - Estrogen receptor
Page 23 and 24: Less robust Abs - Estrogen receptor
Page 25 and 26: Platform dependent antibodies Antig
Page 27 and 28: Platform dependent antibodies - PAX
Page 29 and 30: Inappropriate antibody dilution - C
Page 31 and 32: Inappropriate antibody dilution - I
Page 33 and 34: Inappropriate retrieval - CK AE1/AE
Page 35 and 36: Inappropriate vendor information Mi
Page 37 and 38: Package inserts RP2/18/22 X16/99 Da
Page 39 and 40: Melan A, clone A-103 in high expres
Page 41 and 42: EpCAM Normal kidney UltraView, EP4,
Page 43 and 44: Nordic immunohistochemical Quality
Page 45: Nordic immunohistochemical Quality

large - NordiQC

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