ROMER LABS
ROMER LABS
ROMER LABS
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<strong>ROMER</strong> <strong>LABS</strong><br />
Richard Fielder, Markus Kainz<br />
Tel.: 0845 519 5010<br />
enquiry@romerlabs.com<br />
www.romerabs.com<br />
October 27th<br />
# 1<br />
| © Romer Labs
Check Sample Survey<br />
CSS<br />
October 27th<br />
# 2<br />
| © Romer Labs
Definition<br />
• Global organised survey<br />
• Mycotoxins & Allergens<br />
• Not an accredited PT-scheme<br />
• Improve existing performance<br />
• Free participation for PA customers<br />
September 20th<br />
# 3<br />
| © Romer Labs
Deoxynivalenol (DON)<br />
CSS performed 2010<br />
• Naturally contaminated wheat sample<br />
• Tested for homogeneity<br />
• Dispatched to 164 participants<br />
• 86% participants reported results<br />
September 20th<br />
# 4<br />
| © Romer Labs
Deoxynivalenol (DON)<br />
CSS performed 2010<br />
Participants by country<br />
September 20th<br />
# 5<br />
| © Romer Labs
Deoxynivalenol (DON)<br />
CSS performed 2010<br />
Participants by industry<br />
September 20th<br />
# 6<br />
| © Romer Labs
Deoxynivalenol (DON)<br />
CSS performed 2010<br />
Methods employed<br />
September 20th<br />
# 7<br />
| © Romer Labs
Summary of Results<br />
Analyte<br />
Assigned<br />
Value<br />
[µg/kg]<br />
Number of<br />
satisfactory<br />
results<br />
Number of<br />
questionable<br />
results<br />
Total<br />
number<br />
of results<br />
%<br />
satisfactory<br />
DON 877 122 5 142 86%<br />
September 20th<br />
# 8<br />
| © Romer Labs
G12 ELISA<br />
AgraQuant ®
Regulatory Thresholds
Gluten Methods<br />
Toxicity of foodstuffs<br />
to Coeliac patients<br />
Detect the<br />
presence of<br />
toxic<br />
peptides<br />
Anderson et al, Nat Med 2000;6:337‐342<br />
Arentz‐Hansen et al. J Exp Med 2000; 191:603‐621<br />
Shan et al. Science 2002; 297: 2275‐2279
AgraQuant ® Gluten G12<br />
Toxicity of foodstuffs<br />
A peptide fragment of 33 residues<br />
Remarkably potent T cell stimulator<br />
Morón et al., PLoS ONE 2008 May 28;3(5):e2294.<br />
Morón et al., Am J Clin Nutr. 2008 Feb;87(2):405‐14.<br />
G12 Antibody Specificity<br />
QPQLPY (gliadin ‐ wheat)<br />
QPQQPY (secalin –rye)<br />
QPQLPF (hordein –barley)
AgraQuant ® – LOD & LOQ<br />
Limit of Detection, LOD:<br />
Limits of Quantitation, LOQ:<br />
4 – 200 ppm gluten
Comparative Data
Reactivity to Oats
KEY MESSAGES
Validation & Verification<br />
AGRASTRIP ®<br />
On‐site<br />
tests<br />
AGRAQUANT ®<br />
ELISAs<br />
PCR<br />
Accredited<br />
external lab<br />
Use a combination of testing options
Thank you!<br />
Making the World‘s Food Safer ®<br />
www.romerlabs.com<br />
Tel.: 0845 519 5010<br />
enquiry@romerlabs.com<br />
October 27th<br />
# 18<br />
| © Romer Labs
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