Cetrimide Broth

Selective enrichment of following microorganism Media Positive test organism Page 

Escherichia coli m-Total Count Media Escherichia coli ATCC 25922 

MI-Broth Escherichia coli ATCC 25922 

Tryptic Soy Broth Escherichia coli ATCC 25922 

M -Endo Coliform Broth Escherichia coli ATCC 25922 

Total Count Media with TTC Escherichia coli ATCC 25922 

65 

Coliform MacConkey with MUG Escherichia coli ATCC 25922 47 

M-FC/ M-FC Rosolic Acid Escherichia coli ATCC 25922 51 

Fecal Streptococci KF-Streptococcus Broth Streptococcus faecalis ATCC 19433 45 

Pseudomonas aeruginosa Cetrimide Broth Pseudomonas aeruginosa ATCC 10145 39 

in purified water Pseudomonas Broth Pseudomonas aeruginosa ATCC 10145 60 

Staphylococci Mannitol Salt Broth Staphylococcus aureus ATCC 25923 48 

Enterococci Enterococcus Broth Streptococcus faecalis ATCC 19433 42 

57 

55 

66 

50 

Soft drinks 


Escherichia coli Wallerstein Differential Broth Escherichia coli ATCC 25922 69 

Coliform M -Endo Coliform Broth Escherichia coli ATCC 25922 50 

Yeast and Mold M-Green Select Broth Saccharomyces cerevisiae ATCC 9763 53 

M-Green Yeast and Mold Saccharomyces cerevisiae ATCC 9763 54 

Lactobacillus acidophilus Orange Serum Broth Lactobacillus acidophilus ATCC 314 58 

Beer and wine 



Coliform M -Endo Coliform Broth Escherichia coli ATCC 25922 50 

Yeast and Mold M-Green Select Broth Saccharomyces cerevisiae ATCC 9763 53 

Wallerstein Nutrient Broth Saccharomyces cerevisiae ATCC 9763 69 

36

Products 

Media 

Quick Media Selection Guide 

Quick Media Selection Guide 

Dairy products 




Yeast and Mold Potato Dextrose Broth Saccharomyces cerevisiae ATCC 9763 59 

Lactobacillus MRS Broth Lactobacillus plantarum ATCC 8014 56 

Food 


Escherichia coli Tryptic Soy Broth Escherichia coli ATCC 25922 66 

MIBlue Escherichia coli ATCC 25922 

55 



Yeast and Mold Potato Dextrose Broth Saccharomyces cerevisiae ATCC 9763 59 

Lactobacillus MRS Broth Lactobacillus plantarum ATCC 8014 56 

Pharmaceutical and cosmetics 


Escherichia coli Tryptic Soy Broth Escherichia coli ATCC 25922 66 

M-Endo Coliform Broth Escherichia coli ATCC 25922 50 



Yeast and Mold Sabouraud Dextrose Broth Saccharomyces cerevisiae ATCC 9763 62 

Staphylococci Mannitol Salt Broth Staphylococcus aureus ATCC 25923 48 

Pseudomonas aeruginosa Cetrimide Broth Pseudomonas aeruginosa ATCC 10145 39 

Pseudomonas Broth Pseudomonas aeruginosa ATCC 10145 60 

37

Media 

Brilliant Green Bile Broth 2 % 

Products 

Organisms 

Characteristics 

E. coli ATCC 25922 Growth / gas 

E. aerogenes ATCC 13048 Growth / gas 

E. faecalis ATCC 29212 Inhibited 

S. aureus ATCC 25923 inhibited 

Additional information: 

To prevent the growth of accompanying microorganisms in this media an 

increased concentration of brilliant green should be added. 

Salmonella, for example, are not able to ferment either lactose or 

sucrose. For this reason the lactose contained in this medium allows 

identification of accompanying, weakly lactose-positive or lactose-negative 

organisms. 

Order information Brilliant Green Bile Media 

Product Description Qty/Pkg Order No 

Bottled broth 9 ml vial, Durham tube 20 10 496 710 

38

Products 

Media 

Cetrimide Broth 

Cetrimide Broth 

Used for the isolation and determination 

of Pseudomonas aeruginosa. 

Cetrimide Broth complies with the 

recommendations of the United States 

Pharmacopeia and also European 

Pharmacopeia. The formulation of 

this medium corresponds to the 

specifications in the DIN Norm 38411. 

Description: 

Pseudomonas aeruginosa is characterized 

by the production of pyocyanin (a 

blue green, water soluble, nonflourescent, 

phenazine pigment), which is 

stimulated by the inclusion of magnesium 

chloride and potassium sulfate in 

the broth. 

Cetrimide (N-cetyl-NNN-trimethylammonium 

bromide) is added to inhibit 

bacteria other than Pseudomonas aeruginosa. 

Its action as a quaternary ammonium 

cationic detergent causes nitrogen 

and phosphorus to be released from 

bacterial cells other than Pseudomonas 

aeruginosa. 

Quality Control and recommended 

incubation conditions: 

Positive control: 

Pseudomonas aeruginosa ATCC 10145, 

incubated 24–48 hours at 35º C. 

Negative control: 

Escherichia coli ATCC 25922, 

24–48 hours at 35º C. 

Sterility: 

7 days plated sterility test. 

Cetrimide Broth: Pure Culture of Pseudomonas 

aeruginosa ATCC 10145. 

Formulation: 

Per liter of Water adjusted 

to pH 7.2 ± 0.2 

Peptone 

Magnesium chloride 

Potassium sulfate 

Cetrimide 

Glycerol 

20.0 g 

1.4 g 

10.0 g 

0.3 g 

10.0 ml 

Interpretation: 

Pyocyanin blue-green pigmentation 

surrounding growth is positive for 

Pseudomonas aeruginosa. No color 

development is negative for Pseudomonas 

aeruginosa. 

Organisms 

Characteristics Coloring 

P. aeruginosa ATCC 10145 Growth Blue/green 

E. coli ATCC 25922 inhibited 

S. aureus ATCC 25923 inhibited 

Historical background: 

Harper and Canton followed by Hood described the use of cetrimide (cetyl-trimethylammonium 

bromide) for selective isolation of Pseudomonas aeruginosa. 

Lawbury used cetrimide in a 0.1 % concentration for clinical application. 

Sawbury and Collins later reported a modification of the concentration of 

cetrimide required for selectivity. The introduction of ”Cetevlon“ (cetrimide) 

stimulated a new study to determine the minimum concentration of cetrimide 

required for selective isolation of Pseudomonas aeruginosa for mixed clinical 

flora. The new experiments demonstrated that a concentration of 0.03 % using 

the much improved Cetavlon was sufficient for selectivity. 

Brown and Sawbury introduced the use of a new improved cetrimide agar in 

1965. By combining the Medium B of King, Ward and Raney with the 0.03 % 

cetrimide concentration previously introduced, they developed a medium that 

would support the grow of most desired organisms. 

Order information Cetrimide Broth 


Ampouled Media 2 ml 50 10 496 146 

Bottled broth 50 ml 8 10 496 856* 

Petri dishes with sterile pads 47 mm 100 10 498 544 


* available on request 

39

Media 

EC Broth 

Products 

Organisms 

Growth at 44.5º C 


E. aerogenes ATCC 13048 Growth / no gas 

E. faecalis ATCC 29212 inhibited 

Organisms 

Growth at 37º C 


E. aerogenes ATCC 13048 Growth / gas 



EC Broth was developed by Hajna and Perry for use in the detection of 

coliform bacteria at 37° C and Escherichia coli at 44.5° C. This buffered 

lactose broth was designed to improve the methods of detection of 

contamination of waters, milk and shellfish. Bile salts were incorporated to 

inhibit the growth of gram-positive cocci and sporeformers which 

frequently were responsible for false-positive results obtained when using 

lactose broth or lauryl tryptose broth. The EC Broth formula conforms to 

that recommended by the APHA for use in determining the source of water 

pollution. Through employment of the elevated temperature confirmatory 

test procedure, differentiation can be made between coliforms of fecal 

origin (intestine of warm-blooded animals) and coliforms from other 

sources 

Order information EC Media 


Bottled broth 9 ml, vials, Durham tubes 20 10 496 714 

40

Products 

Media 

EC Broth with MUG 

EC Broth with MUG 

Used for the detection of Escherichia 

coli in water and food samples by a 

fluorogenic procedure. 


The presence of Escherichia coli is 

detected by the appearance of 

fluorescence throughout the tube. 





incubated 24 hours at 35–37º C 


Enterobacter aerogenes ATCC 13048, 

incubated 24 hours at 35–37º C. 

Sterility: 


EC-Broth: Vial left: Control; Vial right: Broth inoculated 

with Escherichia coli ATCC 25922. 

Formulation: 

Per liter of water adjusted 

to pH 6.9 ± 0.2 

Description: 

The presence of the fluorescence using 

a long-wave UV light source confirms 

the presence of Escherichia coli, and 

any further confirmation is not required. 

MUG detects anaerogenic strains, 

which may not be detected in the 

conventional procedure. 

Lactose is a source of energy. Casein 

peptone provides additional nutrients. 

The mixture of bile salts is inhibiting for 

gram-positive bacteria, particularly 

bacilli and fecal streptococci. 

The substrate 4-methylumbelliferyl-β- 

D-glucuronide is hydrolyzed by an 

enzyme, β-glucuronidase, possessed 

by most Escherichia coli and a few 

strains of Salmonella, Shigella and 

Yersinia, to produce a fluorescent end 

product, 4-methylumbelliferone. 

Pancreatic Digest of Casein 

Lactose 

Bile Salts Mixture 

Dipotassium Phosphate 

Monopotassium Phosphate 

Sodium Chloride 

4-Methylumbelliferylβ-D-glucuronide 

20.0 g 

5.0 g 

1.5 g 

4.0 g 

1.5 g 

5.0 g 

50 mg 

Organisms 

Growth at 44,5° C 

E. coli ATCC 25922 Growth/gas/ fluorescence 

E. aerogenes ATCC 13048 inhibited fluorescence 


Organisms 

Growth at 35° C 

E. coli ATCC 25922 Growth/gas/ fluorescence 

E. aerogenes ATCC 13048 Growth/gas/ no fluorescence 


Order information EC Media with MUG 


Bottled broth 9 ml, vial 20 10 496 709 

Bottled broth 9 ml vial, no Durham Tubes 20 10 496 332 

41

Media 

Enterococcus Broth 

Products 

Enterococcus Broth 

Selected media for use in membrane 

filtration procedures for the isolation 

and enumeration of enterococci in 

food, water and other materials. 

Description: 

Enterococcus broth is a modified version 

of the improved media described 

by Slanetz and Bartley with TTC. The 

membrane filtration method is simple to 

perform, does not require confirmation 

and permits a direct count of enterococci 

in 48 hours. 


Enterococci appear as pink to dark 

maroon colonies from 0.5–3 mm in diameter. 

Organism 

Growth/Coloring 

E. faecalis ATCC 19433 Pink to red colonies 

E. coli ATCC 25922 Inhibited 



Positive control. 

Enterococcus faecalis ATCC 19433, 

incubated at 35º C for 24 hours. 


Escherichia coli ATCC 25922 

incubated at 35º C for 24–48 hours. 

Sterility test: 


Enterococcus Broth: A pure culture of Enterococcus 

faecalis ATCC 19433 appears pink to red on 

this media. 

Formulation: 


to pH 7.2 ± 0.2 

Yeast extract 

Casein 

Dextrose 

Papaic digest of soybean meal 

Potassium phosphate 

Sodium azide 

Triphenyltetrazoliumchloride1% 

5.0 g 

15.0 g 

2.0 g 

5.0 g 

4.0 g 

0.4 g 

10 ml 

Additional informations: 

The presence of sodium azide function as inhibitor for the growth of the 

entire accompanying Gram-negative microbial flora. As described above 

Enterococci reduce TTC and therefore, their colonies appear pink to dark 

maroon in color. Furthermore an improved selectivity for enterococci can 

be obtained by adding additives like carbonate and Tween 80 ® to the 

media (Lachica and Hartman,1968). 

Order information Enterococcus Media 






42

Products 

Media 

Eugon Broth 

Eugon Broth 

Used for the cultivation of a wide 

variety of microorganisms, including 

fastidious bacterial species. 

Description: 

Eugon media was developed to obtain 

eugonic (luxuriant) growth of fastidious 

microorganisms. The unenriched media 

supports rapid growth of lactobacilli 

associated with cured meat products, 

dairy products and other food. 

The high concentration of Dextrose is 

the energy source for rapid growth of 

bacteria. L-cystine and sodium sulfite 

are added to stimulate growth. Sodium 

chloride maintains the osmotic balance 

of the media. The high carbohydrate 

content along with high sulfur (cystine) 

content improves growth with chromogenicity. 


Colony morphology and color are species 

specific. 





24–48 hours at 35–37º C 

Candida albicans ATCC 10231, 

48 hours at 25–30º C 


Not undertaken. 

Sterility: 


Eugon Broth: A pure culture of Escherichia coli 

ATCC 25922 appears on Eugon Broth with typical 

white-creamy to opaque colonies. 

Formulation: 


to pH 7.0 ± 0.2 


Papaic Digest of Soybean Meal 


l-Cystine 

Sodium Sulfite 

Dextrose 

15.0 g 

5.0 g 

4.0 g 

0.3 g 

0.2 g 

5.5 g 

Organism 

Coloring 

E. coli ATCC 25922 White, 

cream to 

opaque 

colonies 

Order information Eugon Media 







43

Media 

HPC Broth with TTC 

Products 

HPC Broth with TTC 

Used for heterotrophic plate counts 

in the examination, for example, of 

potable water, dairy products and 

swimming pools. 

Description: 

HPC is used to determine total count at 

incubation temperatures of 35º C. All 

bacteria develop on HPC with indicator 

media and produce a red color as a 

result of the precipitation of formazan 

following the reduction of 2,3,5- 

triphenyltetrazolium chloride (TTC) by 

bacteria. 





incubate for 24–48 hours at 35º C. 



Sterility: 


HPC with Indicator: Escherichia coli ATCC 25922 

shows a typical red to pink coloring. 

Formulation: 


to pH 7.1 ± 0.2 


Colonies that develop on HPC are 

counted as total heterotrophic counts. 

Specific colony identification should be 

done using standard microbiological 

identification techniques. 

Peptone 

Gelatin 

Glycerol 

TTC, 1% solution 

30.0 g 

15.0 g 

15.0 ml 

10.0 ml 

Organisms 


E. coli ATCC 25922 Growth Pink to red 

E. faecalis ATCC 29212 Growth Pink to red 

S. aureus ATCC 25923 Growth Pink to red 

Order information HPC with TTC 






44

Products 

Media 

KF-Streptococcus Broth 

KF-Streptococcus Broth 

Selected media for the isolation and 

enumeration of fecal streptococci 

Description: 

KF-Streptococcus Broth is selective for 

the determination of fecal streptococci 

in polluted surface waters. Maltose and 

lactose are fermentable carbohydrates, 

sodium azide is the selective agent and 

brom cresol purple is the indicator dye. 


Identification of fecal streptococci has 

to be undertaken using conventional 

microbiology techniques. 

Organisms 


E. faecalis ATCC 29212 Growth Red 

E. faecalis ATCC 19433 Growth Red 

E. aerogenes ATCC 13048 inhibited 





Enterococcus faecalis ATCC 19433, 

incubated 24–48 hours at 35° C. 



incubated 24– 48 hours at 35° C. 

Sterility: 


KF-Streptococcus Broth: Pure culture of Enterococcus 

faecalis ATCC 19433 develops on this 

media a typical red colony coloring. 

Formulation: 


to pH 7.2 ± 0.2 

Peptone 

Yeast extract 

Sodium chloride 

Sodium glycerophosphate 

Maltose 

Lactose 

Sodium azide 

Brom cresol purple 

TTC 1% solution 

10.0 g 

10.0 g 

5.0 g 

10.0 g 

20.0 g 

1.0 g 

0.4 g 

15 mg 

10.0 ml 

Order information KF-Streptococcus Media 




100 ml 1 10 496 754* 

500 ml 1 10 496 755* 




45

Media 

Lauryl Sulfate or Lauryl Tryptose Broth 

Products 

Lauryl Sulfate or Lauryl Tryptose Broth 

Used for the detection of coliform 

organisms in water, wastewater and 

foods. 

Description: 

This media was developed for the 

detection of coliform organisms by the 

American Public Health Association 

(APHA). It is now the standard medium 

of choice in the presumptive phase of 

the standard coliform MPN test for the 

microbiological examination of water. 


Lactose acts as a source of fermentable 

carbohydrates for coliforms. The 

fermentation of lactose with gas formation 

is a presumptive test for coliforms. 

Sodium lauryl sulfate inhibits the 

growth of organisms other than coliforms. 





incubation for 24–48 hours at 35º C. 


Enterococcus faecalis ATCC 19433 

incubated at 35º C for 24–48 hours = 

no growth. 

Sterility test: 


Lauryl Sulfate Broth: Pure culture of Escherichia 

coli ATCC 25922 cultivated on this media shows 

normal growth with gas formation. 

Formulation: 


to pH 6.8 ± 0.2 

Sodium lauryl sulfate 


Lactose 

Dipotassium phosphate 

Monopotassium phosphate 


0.1 g 

20.0 g 

5.0 g 

2.75 g 

2.75 g 

5.0 g 

Organisms 


E. coli ATCC 25922 Growth/gas 

E. aerogenes ATCC 13048 Growth/gas 

S. faecalis ATCC 29212 inhibited 

Order information Lauryl Sulfate or Lauryl Tryptose Media 


Lauryl Sulfate or Lauryl Tryptose Broth 9 ml vials 20 10 496 722 




46

Products 

Media 

MacConkey with MUG 

MacConkey with MUG 

Used in the presumptive phase for 

the presence of coliform bacteria in 

water and foods. Selective to the 

detection of gram-negative lactose 

fermenting bacilli. This medium 

largely complies with the European 

Pharmacopeia. 

Description: 

MacConkey Broth was originally 

developed by MacCONKEY and HILL 

(1901) and the formulation for the agar 

was modified by MacConkey in 1950. 

MacConkey developed this medium for 

the cultivation of enteric pathogens and 

coliforms. It contains bile salts, which 

inhibit certain gram-negative bacteria, 

and crystal violet, which inhibits grampositive 

organisms such as enterococci 

and staphylococci. Combination of the 

lactose and neutral red indicator to 

produce pink to red colored colonies 

indicates an isolate with the ability to 

ferment lactose. Non-lactose fermenting 

organisms are colorless. The addition 

of MUG (4-Methylumbellifryl-β-Dglucuronide), 

which is a fluorogenic 

enzyme, allows the media to selectively 

identify Escherichia coli. MUG is 

hydrolyzed by the Escherichia coli 

specific enzyme β-glucuronidase to release 

4-Methylumbellifone which fluoresces 

under ultraviolet light. 


Fluorescence under UV light is specific 

for the presence of Escherichia coli. 

Lactose fermenting organisms grow as 

pink to red colonies. 

Organisms Characteristics Coloring 


S. typhimurium ATCC 14028 Growth Clear 

E. coli ATCC 25922 Growth/fluorescence 





incubated at 35º C for 24 hours. 

Checked for florescence at 366 nm. 



Sterility. 


MacConkey with MUG: Escherichia coli ATCC 

25922 forms as lactose-fermenting organisms pink 

to red colonies. 

Formulation: 

Make to 1 liter and adjust pH to 7.1 

Casein Peptone 

17.0 g 

Proteose Peptone 

3.0 g 

Lactose 10.0g 

Bile Salts #3 

1.5 g 


5.0 g 

Neutral Red 

30 mg 

Crystal Violet 

0.1 mg 

MUG (4-Methylumbelliferylβ-D-glucuronide) 

0.1 g 


MacConkey Broth is a modification of the original bile salt broth where bromo 

cresol purple is used in place of neutral red or litmus as the indicator. Bile salts 

replace 0.5 % sodium taurocholate in the origin formulation. The addition of MUG 

(4-Methylumbellifryl-(-D-glucuronide), which is a fluorogenic enzyme, allows the 

media to selectively identify Escherichia coli. MUG is hydrolyzed by the 

Escherichia coli specific enzyme (β-glucuronidase to release 4-Methylumbellifone 

which fluoresces under ultraviolet light). 


MacConkey Broth is a modification of the formula given by MacConkey which 

corresponds to the alternative formulation recommended by the World Health 

Organization. The medium is used for the presumptive determination of the 

presence of coliform organisms (gram-negative, lactose – fermenting bacilli) in 

water and milk as well as other materials. Presence of lactose – fermenting 

organisms is detected by the change of color of the medium (from purple to 

yellow) after inoculation and incubation.The original formula of MacConkey called 

for use of litmus as an indicator of acid reaction. In later investigations neutral red 

was found to be a more suitable indicator. More recently, Childe and Allen 

demonstrated an inhibitory effect on the growth of Escherichia coli by neutral red 

in this medium. Bromcresol purple is not only less inhibitory, but also gives a 

more clear – cut indication of acid reaction. 

Order information MacConkey with MUG 






47

Media 

Mannitol Salt Broth 

Products 

Mannitol Salt Broth 

Used for the selective isolation and 

enumeration of staphylococci. In 

addition the medium complies with 

the recommendations in the United 

States Pharmacopeia. 

Description: 

Because of the amount of peptones 

and beef extract, Mannitol Salt is a 

nutrient rich medium. Most bacteria 

(other than staphylococci) are inhibited 

by the high concentration of sodium 

chloride. Organisms capable of fermenting 

mannitol e.g. Staphylococcus 

aureus, cause a pH change in the 

media. With phenol red as the pH 

indicator the colonies appear with a 

yellow coloration. 


Typical pathogenic staphylococci 

ferment mannitol and form yellow 

colonies with yellow zones, while 

typical non-pathogenic staphylococci 

do not ferment mannitol and form red 

colonies. 




Staphylococcus aureus ATCC 25923, 





Sterility: 


Appearance of Colonies 

Surrounded by bright 

yellow zones, abundant 

growth 

pink to red colonies 

growth is usually poorer 

Microorganisms 

Mannitol-positive: 

S. aureus 

Mannitol-negative: 

S. epidermis and others 

Mannitol Salt Broth: Staphylococcus aureus ATCC 

25923 forms typical yellow colonies with zones on 

this media. Indication for Mannitol positive 

organisms. 

Formulation: 


to pH 7.4 ± 0.2 

Beef Extract 


Peptic Digest of Animal Tissue 


d-Mannitol 

Phenol Red 

1.0 g 

5.0 g 

5.0 g 

75.0 g 

10.0 g 

25 mg 

Organisms 


S. aureus ATCC 25923 Growth Yellow with 

yellow zones 

S. epidermidis ATCC 12228 Growth Red with 

no zone 


E. aerogenes ATCC 13048 inhibited 


The tolerance of Staphylococcus aureus to high concentrations of sodium 

chloride was reported by Koch in 1942. In 1945, Chapman described a 

formulation which incorporated 7.5 % sodium chloride in phenol red 

mannitol agar to successfully cultivate pathogenic staphylococci. These 

coagulase positive organisms grew in large colonies surrounded by yellow 

zones. Non-pathogenic staphylococci are usually less luxuriant after the 36 

hour incubation period recommended by Chapman. 

Mannitol Salt Broth and Agar is recommended for the enumeration of 

staphylococci in food and dairy products by the American Public Health 

Association. 

Order information Mannitol Salt Media 






48

Products 

Media 

Membrane Lauryl Sulfate Broth 

Membrane Lauryl Sulfate Broth 

For the presumptive identification of 

coliforms and Escherichia coli in water 

and drinking water. Prepared to the 

formula published in Journal of 

Hygiene (PHLS/SCA). 

Description: 

This media was developed for the 

detection of coliform organisms and is 

now the media of choice for the 

enumeration of total coliforms and 

Escherichia coli in the United Kingdom. 

This media replaced membrane enriched 

broth containing 0.4 % Teepol 610. 

To use membrane lauryl sulphate broth 

for the identification of thermotolerant 

coliforms incubate at 30º C for 4 hours 

then incubate for further 14 hours at 

44º C. Yellow colonies are counted as 

presumptive coliforms which require 

confirmation. 

Organisms 


37 °C 

E. coli ATCC 25922 Growth Yellow 

E. aerogenes ATCC 13048 Growth Yellow 

E. faecalis ATCC 29212 Growth Pink or 

colorless 

This picture shows a mix culture on Membrane 

Lauryl Sulphate Broth incubated at 37° C. Organisms 

like Escherichia coli ATCC 25922 and Enterobacter 

aerogenes ATCC 13048 form yellow 

colonies whereas Enterococcus faecalis ATCC 

29212 appears as pink colonies. 


Lactose acts as a source of fermentable 

carbohydrates for coliforms. Phenol 

red acts as an indicator of acidity as a 

result of coliform metabolism. Incubate 

for 4 hours at 30º C then increase the 

temperature to 37º C and incubate for 

further 14 hours. Yellow colonies are 

counted as presumptive coliforms which 

require confirmation. Pink or colorless 

colonies are not counted as coliforms. 

Organisms 


44 °C 

E. coli ATCC 25922 Growth Yellow 

E. aerogenes ATCC 13048 Inhibited Inhibited 

E. faecalis ATCC 29212 Inhibited Inhibited 





incubation for 4 hours at 30º C 

then for 14 hours at 37º C. 

Formulation: 


to pH 7.4 ± 0.2 

Peptone 

Yeast extract 

Lactose 

Phenol red (0.4% solution) 


40.0 g 

6.0 g 

30.0 g 

50.0 ml 

1.0 g 



Sterility: 


Order information Membrane Lauryl Sulfate Media 






49

Media 

M-Endo Coliform Broth 

Products 

M-Endo Coliform Broth 

M-Endo Broth is used for the 

enumeration of coliforms by membrane 

filtration. It is used to differentiate 

lactose from non-lactose 

fermenting intestinal organisms and 

as a presumptive test for coliforms. 

Description: 

M-Endo is a red colored media, which 

needs to be stored in the dark to prevent 

discoloration of the media. Gram-positive 

bacteria are inhibited on this media by 

the desoxycholate and lauryl sulfate. The 

addition of ethanol increases the 

antibacterial nature of the formulation. 

Lactose fermenting organisms form 

aldehydes, which react with Schiffs 

reagent (basic fuchsin and sodium sulfite) 

to give red colored zones around the 

colonies. Coliform colonies are therefore 

red with a characteristic metallic sheen. 


Production of both acid and aldehyde 

by lactose fermenters, such as 

Escherichia coli, produce deep red 

colonies that color the surrounding 

medium and have a green metallic 

sheen. Non-lactose fermenters form 

colorless, translucent colonies. 

Organisms 


E. coli ATCC 25922 Growth Red with 

green 

metallic 

sheen 

E. aerogenes ATCC 13048 Growth Red colonies 

with or 

without 

green 

metallic 

sheen 

P. aeruginosa ATCC 10145 Growth Pink to 

colorless 

S. aureus ATCC 25923 Marked to 

complete inhibition 





24 hours at 35º C. 




Sterility: 


M-Endo Broth: Mixed culture of Escherichia coli 

ATCC 25922: (red colonies with green metallic 

sheen) and Pseudomonas aeruginosa ATCC 10145 

(pink to colorless). 

Formulation: 


to pH 7.2 ± 0.2 

Peptones 

10.0 g 

(equal parts digests of animal tissue 

and casein) 

Peptic Digest of Animal Tissue 5.0 g 

Pancreatic Digest of Casein 5.0 g 

Yeast extract 

1.5 g 

Lactose 

12.5 g 


5.0 g 

Dipotassium phosphate 4.375 g 

Monopotassium phosphate 1.375 g 


50 mg 

Sodium desoxycholate 

0.1 g 

Sodium sulfite 

2.1 g 

Basic fuchsin 

1.05 g 

95 % Ethanol 30.0 ml 

Order information M-Endo 



Bottled broth 50 ml, Screw cap 8 10 496 700 

50 ml, Septa cap 8 10 496 701 




50

Products 

Media 

M-FC Broth 

M-FC Broth 

M-FC media (membrane Fecal 

Coliform media) is used for the detection 

of fecal coliforms as an index 

of water pollution. 

Description: 

Allows the development of fecal 

coliforms at elevated temperatures 

(44.5° C). 


Bile salts included in the medium inhibit 

the growth of gram-positive bacteria. 

Fecal coliforms ferment lactose at 

elevated temperatures and produce 

blue colonies. Other organisms form 

grey to cream colonies. 

Organisms 


E. coli ATCC 25922 Growth Blue 

E. aerogenes ATCC 13048 Growth Gray to 

cream 






incubated 24 hours at 44.5º C. 



incubated 24 hours at 44.5º C. 

Sterility: 


M-FC-Media: A pure culture of Escherichia coli 

ATCC 25922 shows a typical blue coloring on 

m-FC Broth. 

Formulation: 


to pH 7.4 ± 0.2 

Tryptose 

Peptone No. 3 

Yeast extract 


Lactose 

Bile salts 

Aniline blue 

10.0 g 

3 5.0 g 

3.0 g 

5.0 g 

12.5 g 

1.5 g 

0.1 g 

Order information M-FC-Media 

M-FC-Media: A cultivated mix culture indicates 

lactose fermenters as blue colonies whereas nonlactose 

fermenters form grey to creamy colonies 

e.g. Enterobacter aerogenes ATCC 13048. 




100 ml 1 10 496 757* 

500 ml 1 10 496 758* 




51

Media 

M-FC with Rosolic Acid 

Products 

M-FC with Rosolic Acid 

M-FC Broth with Rosolic Acid is used 

for the detection of fecal coliforms by 

the membrane filtration technique. 

Description: 

M-FC with Rosolic Acid acts and 

functions in the same way as m-FC 

Broth. Rosolic acid inhibits bacterial 

growth in general, except for fecal 

coliforms. 


Bile salts inhibit non-enteric bacteria. 

Aniline blue indicates the ability of fecal 

coliforms to ferment lactose to acid 

that causes a pH change in the 

medium. Other organisms form grey to 

cream colonies. 

Organisms 


E. coli ATCC 25922 Growth Blue 

E. aerogenes ATCC 13048 Growth Gray to 

cream 






24 hours at 44,5º C. 



24 hours at 44.5º C. 

Sterility: 


M-FC with Rosolic Acid: Escherichia coli ATCC 

25922 forms blue colonies whereas non-lactose 

fermenters appears as grey colonies. 

Formulation: 

Per liter of water adjusted to pH 7.4 

Tryptose 

Peptone No. 3 

Yeast extract 


Lactose 

Bile salts 

Aniline blue 

Rosolic Acid 1% 

10.0 g 

5.0 g 

3.0 g 

5.0 g 

12.5 g 

1.5 g 

0.1 g 

10.0 ml 


Fecal coliform (FC) Medium for the membrane filtration technique was 

described by Geldereich et al. in 1965. It was the first membrane filtration 

technique to be incubated at 44.5 ± 0.2. 

Order information m-FC with Rosolic Acid 







52

Products 

Media 

M-Green Select Broth 

M-Green Select Broth 

Used for the enumeration of yeasts 

and mold in soft drinks and fruit 

juices. 

Description: 

M-Green Select Broth is an improved 

modification of the liquid medium, m- 

Green Yeast and Mold Broth and was 

developed to improve efficiency of 

detection and enumeration of fungi in 

sugar based drinks using the membrane 

filtration method. 

This medium has a low pH, which 

inhibits bacterial growth. The addition 

of Chloramphenicol further inhibits the 

growth of bacteria to allow for the development 

and enumeration of yeast 

and mold. 

The addition of bromocresol green, 

which diffuses into fungal colonies as 

an alkaline reaction, allows them to be 

easily identified. Metabolic by-products 

from the developing colonies diffuse 

into the surrounding medium, further 

reducing the pH which aids in the 

inhibition of bacterial growth, but also 

produces an acid reaction which 

causes residual bromocresol green to 

change to yellow. 


Green opaque colonies against a 

yellow background indicative of the 

growth of yeast. Mold colonies are 

green and filamentous. 








incubated 48 hours at 35º C. 

Sterility: 


M-Green Select Media: Ideal for the enumeration 

of yeasts & mold. 

Formulation: 


to pH 4.6 ± 0.2 

Dipeptone 

Yeast extract 

Dextrose 

Magnesium sulfate 


Diastase 

Thiamine 

Bromocresol green 

Chloramphenicol, 1% Solution 

10.0 g 

9.0 g 

50.0 g 

2.1 g 

2.0 g 

50 mg 

50 mg 

26 mg 

8.5 ml 

Organisms 


E. coli ATCC 25922 Partial to marked inhibition 

S. cerevisiae ATCC 4098 Growth 

C. albicans ATCC 10231 Growth 

Order information M-Green Select Media 







53

Media 

M-Green Yeast and Mold 

Products 

M-Green Yeast and Mold 

Used for the enumeration of yeast 

and mold in soft drinks and fruit 

juices. 

Description: 

M-Green is an improved modification of 

the liquid medium, m-Yeast and Mold 

Broth and was developed to improve 

efficiency of detection and enumeration 

of fungi in sugar based drinks using the 

membrane filtration method. 

This medium has a low pH, which 

inhibits bacterial growth. The addition 

of bromocresol green, which diffuses 

into fungal colonies as an alkaline 

reaction, allows them to be easily 

identified. Metabolic by-products from 

the developing colonies diffuse into the 

surrounding medium, further reducing 

the pH which aids in the inhibition of 

bacterial growth, but also produces an 

acid reaction which causes residual 

bromocresol green to change to yellow. 


Green opaque colonies against a 

yellow background are indicative of the 

growth of yeast. Mold colonies are 

green and filamentous. Examples of 

detected organisms: 





48 hours at 25–30º C. 



Sterility: 


M-Green Yeast & Mold Broth: Typical growth of 

Candida albicans ATCC 10231 on a black 

membrane. 

Formulation: 


to pH 4.6 ± 0.2 

Dipeptone 

Yeast extract 

Dextrose 



Diastase 

Thiamine 


10.0 g 

9.0 g 

50.0 g 

2.1 g 

2.0 g 

50 mg 

50 mg 

26 mg 

Order information M-Green Yeast & Mold 




100 ml 1 10 496 760* 

500 ml 1 10 496 761* 

Bottled agar 100 ml 1 10 496 705 




54

Products 

Media 

MI Broth and MI Agar 

MI Broth and MI Agar 

Used for the simultaneous detection 

of total coliforms and Escherichia 

coli in water according to the Surface 

Water Treatment Rule (USEPA) and 

the Total Coliform Rule (USEPA) 

Description: 

MI Broth detects the presence of 

coliform bacteria by the production of 

β-galactosidase, which cleaves the 

substrate MUGal to produce 4-Methylumbelliferone, 

which fluoresces on 

exposure to UV light. Non-coliforms do 

not produce this enzyme and therefore 

do not fluoresce on the medium. 

Escherichia coli is detected by the 

compound IBDG. The β-glucuronidase 

produced by Escherichia coli cleaves 

the substrate to produce a blue indigo 

color in the colonies. As Escherichia 

coli is also a total coliform, and also 

produces β-galactosidase it will also 

fluoresce. 

The antibiotic cefsulodin is added to 

inhibit the growth of gram-positive 

bacteria and some non-coliform gramnegative 

bacteria that can cause false 

positive reactions. 

MIBlue is specially developed for the 

food industry. 


Fluorescent blue colonies are 

Escherichia coli. 

Colonies that demonstrate blue/white 

fluorescence where the colonies are 

clear, cream or pale yellow in color are 

other coliform organisms. Clear 

colonies that do not fluoresce are noncoliforms. 

Organisms 


E. coli ATCC 25922 Growth Blue with 

fluorescence 

E. aerogenes ATCC 13048 Growth Yellow with 

fluorescence 

P. aeruginosa ATCC 10145 Complete 

inhibition 











Sterility: 


MI-Media: Pure Culture of Escherichia coli ATCC 

25922 with UV light. 

Formulation: 


to pH 6.95 ± 0.2 

Protease peptone 

Yeast extract 

β-d-lactose 

MUGal 

NaCl 

K2HPO4 

KH2PO4 


Sodium desoxycholate 

IBDG 

Cefsulodin 

(Agar) 

5.0 g 

3.0 g 

1.0 g 

0.1 g 

7.5 g 

3.3 g 

1.0 g 

0.2 g 

0.1 g 

0.32 g 

5 mg 

15.0 g 

Order information MI Media 

MI-Broth Media: Mixed Culture with Escherichia 

coli ATCC 25922 and Enterobacter aerogenes 

ATCC 13048 without UV. 



Bottled broth 50 ml 1 10 496 851 




MIBlue 10 496 501* 


55

Media 

MRS Broth 

Products 

MRS Broth 

Used for the isolation and cultivation 

of lactobacilli. MRS Broth complies 

with the German DIN-Norm 10109 

and the International Standard ISO 

13721 for the detection of lactosein 

meat and to the regulations acc. to § 

35 LMBG (06.00/35) for the detection 

in meat. 

Description: 

MRS medium supports luxuriant 

growth of all lactobacilli, even the slow 

growing species. 


Lactobaccili appear as white colonies. 

Pediococcus and Leuconostoc may 

also grow on MRS. 




Lactobacillus plantarum ATCC 8014 

Lactobacillus fermentum ATCC 9338 

incubated at 35º C for 48–72 hours. 



Sterility: 


MRS Media; Pure Culture of Lactobacillus plantarum 

ATCC 8014. 

Formulation: 


to pH 6.2 ± 0.2 


Beef extract 

Yeast extract 

Dextrose 

Dipotassium Phosphate 

Polysorbate 80 

Ammonium citrate 

Sodium acetate 


Manganese sulfate 

10.0 g 

8.0 g 

4.0 g 

20.0 g 

2.0 g 

1.0 g 

2.0 g 

5.0 g 

0.2 g 

50 mg 


The media contains special growth factors for lactobacilli like polysorbate, 

acetate, magnesium and manganese. These compounds are known as rich 

nutrient base. On a very low degree of selectivity, Pediococcus and Leuconostoc 

species and other secondary bacteria can be cultivated. 


MRS Agar was developed by de Man, Rogosa & Sharper for the cultivation 

of Lactobacillus species. The medium will effectively support the growth of 

many strains of lactobacilli that do not generally grow as well as other 

media designed for this purpose. Due to the nutritional factors and the 

neutral pH other organisms which are not fastidious will grow on the 

medium. 

Lactobacillus will grow well on the surface of the medium as well as in 

deep culture preparations. Although enrichment with carbon dioxide is 

unnecessary with this medium, the atmosphere must be kept fairly moist 

Order information MRS Media 







56

Products 

Media 

M – TGE Total Count Media 

M – TGE Total Count Media 

Used for the non-selective development 

and enumeration of all aerobic 

bacteria. 

Description: 

All bacteria develop on TGE media and 

produce a range of different colored 

and sized colonies. 


Identification of bacteria should be 

undertaken by using traditional microbiology 

techniques following initial 

colony development. 

For quality control two typical 

organisms are detected and enumerated 

with m – TGE Total Count Media: 

Organisms 


E. coli ATCC 25922 Growth Yellow to 

cream 

S. aureus ATCC 25923 Growth Yellow to 

cream 








Sterility: 


Pure culture of Escherichia coli ATCC 25922 on 

m-TGE Total Count Media. 

Formulation: 

Per liter of water and adjusted 

to pH 7.0 ± 0.2 


Yeast extract 

Dextrose 

10.0 g 

5.0 g 

2.0 g 

Order information M-TGE Total Count 

M-TGE Total Count Media with a mixed culture of 

Escherichia coli ATCC 25922 and Staphylococcus 

aureus ATCC 25923. 




100 ml 1 10 496 763* 

300 ml 1 10 496 764* 

500 ml 1 10 496 765* 




57

Media 

Orange Serum Media 

Products 

Orange Serum Media 

Used for the isolation and enumeration 

of organisms associated with 

the spoilage of citrus juices. 

Description: 

Organisms known to grow in single 

strength and concentrated juices are 

lactic acid and acetic acid bacteria and 

yeast. Lactobacilli, Leuconostoc and 

yeast have all been identified as spoilage 

organisms by numerous authors. 

Orange serum at pH 5.4 to 5.6 has 

been reported to yield maximum 

counts of all types of spoilage 

organisms in mixed cultures, and in 

single culture comparison tests. 




Lactobacillus fermentum ATCC 9338, 



48 hours at 25–30º C. 



Sterility: 


Orange Serum Media agar is especially selective 

for microorganisms which prefer low pH conditions 

e.g. Lactobacillaceae and some yeast e.g. 

Candida. 

Formulation: 

Per liter of water, adjusted 

to pH 5.6 ± 0.2 


The low pH of the test solution usually 

prohibits the development of other 

microorganisms which cannot survive 

low pH conditions. Therefore developing 

colonies are presumed to be 

problematic organisms. 

Orange serum 

Yeast extract 

Tryptone 

Dextrose 


10.0 g 

3.0 g 

10.0 g 

4.0 g 

2.5 g 

Order information Orange Serum 



Bottled Agar 100 ml 1 10 496 713 




58

Products 

Media 

Potato Dextrose Broth and Agar Media 

Potato Dextrose Broth and Agar Media 

Recommended for culturing and 

enumerating yeast and mold. 

Potato Dextrose Broth complies with 

the recommendations of the American 

Public Health Association for food 

and the USP. 

Description: 

Potato Dextrose Broth is recommended 

in Standard Methods as the media that 

gives the most consistent and highest 

counts for the recoveries of yeast and 

mold in dairy products. The inclusion of 

potato extract encourages the growth 

and development of fungi. Sterile 

Tartaric Acid may be added to low the 

pH to 3.5 ± 0.2 to further inhibit the 

growth of conflicting bacteria. 


Yeast, mold and acid tolerant bacteria 

grow well on Potato Dextrose Broth. 

Organisms 


C. albicans ATCC 10231 Growth White, 

creamy 

S. cerevisiae ATCC 4098 Growth White, 

creamy 





incubated at 25–30º C for 48 hours. 



Sterility: 


Potato Dextrose Media: Pure culture of Candida 

albicans ATCC 10231. 

Formulation: 


to pH 5.1 ± 0.2 

Potato infusion 

Dextrose 

For agar add: 

Agar 

4.0 g 

20.0 g 

15.0 g 


In general carbohydrate and potato infusion promote the growth of yeast 

and mold. Additionally the low pH value has a positive effect by partially 

inhibiting the growth of accompanying bacterial flora. If the medium is used 

for fungal counts, the pH should be adjusted to approximately 3.5. Fungi 

grow on this medium to develop typical morphology. 


Potato Dextrose Broth is commonly used in slide culture preparations of 

fungi to stimulate sporulation and to enhance growth of poorly sporulating 

mycelia. It has been used for cultivation and isolation of mold and yeast in 

dairy and food products as recommended by the American Public Health 

Association and for maintenance of stock cultures of geophilic 

dermatophytes. 

Order information Potato Dextrose Media 




100 ml 1 10 496 770* 

500 ml 1 10 496 771* 

Bottled agar 23 ml tube 15 10 496 863* 

100 ml 1 10 496 731* 

500 ml 1 10 496 767* 

1000 ml 1 10 496 768* 




59

Media 

Pseudomonas Broth 

Products 

Pseudomonas Broth 

Used for the isolation of Pseudomonas 

and differentiating Pseudomonas 

aeruginosa from other pseudomonads 

based on pigment formation. 

Pseudomonas Broth complies with 

the formulation recommendations of 

the United States Pharmacopeia and 

to the specifications in the DIN Norm 

38411 (examination of water). 

Description: 

Pseudomonas aeruginosa is characterised 

by the production of pyocyanin (a 

blue green, water soluble, nonflourescent, 

phenazine pigment), which is 

stimulated by the inclusion of magnesium 

chloride and potassium sulfate in 

the broth. Irgasan, an antimicrobial 

agent, selectively inhibits gram positive 

and gram negative bacteria other than 

pseudomonads. Glycerol serves as both 

an energy source and helps in the 

promotion of pyocyanin. 


Development of a green to blue green 

pigmentation surrounding colonies is 

positive for Pseudomonas aeruginosa. 

Other pseudomonads develop as clear 

to amber yellow colonies. Non-pseudomonads 

are suppressed. 

Organisms 


P. aeroginosa ATCC 10145 Growth Blue to 

blue-green 

P. aeroginosa ATCC 27853 Growth Blue to 

blue-green 










Sterility: 


Pseudomonas Media: Typical growth of Pseudomonas 

aeroginosa ATCC 10145. 

Formulation: 


to pH 7.0 ± 0.2 


Magnesium chloride 

Potassium sulfate 

Irgasan 

Glycerol 

20.0 g 

1.4 g 

10.0 g 

0.25 g 

20.0 ml 


Identification of most Pseudomonas strains can be obtained by their 

different pigmentation according to the compounds used in the media. 

Some of the strains can only synthesize pyocyanin, some form only 

fluorescein and others form both pigments. 

Pseudomonas aeruginosa ATCC 27853 is characterised by the production 

of pyocyanin (a blue green, water soluble, nonflourescent, phenazine 

pigment), which is stimulated by the inclusion of magnesium chloride and 

potassium sulfate in the broth. The same Pseudomonas aeruginosa strain 

appears on Pseudomonas Broth as colonies surrounded by a yellow to 

greenish-yellow zone when different types of peptone are added and 

magnesium chloride and potassium sulphate are omitted. 

BLAZEVIC et al. (1973), noted that some Pseudomonas aeruginosa strains 

atypically appear as pyocyanin-negative, fluorescein-positive and for that 

reason it is possible to differentiate them from Pseudomonas fluorescens 

and Pseudomonas putida. Furthermore BRODSKY and NIXON (1973) 

showed that cultivation on MacCONKEY agar can be used for a simple and 

rapid differentiation of these strains. 

Order information Pseudomonas Media 




100 ml 1 10 496 776* 

500 ml 1 10 496 777* 

Bottled agar 50 ml 8 10 496 772* 

100 m 1 10 496 773* 

500 ml 1 10 496 774* 




60

Products 

Media 

R2 Broth and R2 Agar 

R2 Broth and R2 Agar 

Used for Heterotrophic plate counts 

in the examination of potable water. 

Description: 

R2 broth can be used to determine 

heterotrophic plate count at 35ºC. 

When incubated at lower temperatures 

(25–30º C) for longer periods of 72–96 

hours it can also be used to recover 

environmentally stressed organisms, or 

those that are chlorine tolerant. 


Heterotrophic plate counts are not the 

same as standard plate counts and a 

media such as TGE should be run in 

combination with R2. 

Organisms 


E. coli ATCC 25922 Growth 

E. faecalis ATCC 29212 Growth 

S. aureus ATCC 25923 Growth 





incubation for not less than 72 hours at 

35° C. 


Not applicable. 

Sterility: 


Sample of tap water on R2 Media. 

Formulation: 


to pH 7.2 ± 0.2 

Yeast extract 

Peptones 

Acid hydrolysate of casein 

Dextrose 

Soluble starch 



(anhydrous) 

Sodium pyruvate 

0.5 g 

0.5 g 

0.5 g 

0.5 g 

0.5 g 

0.3 g 

24 mg 

0.3 g 

For agar add: 

Agar 

15.0 g 

Order information R2 Media 



Bottled agar 15 ml tube 15 10 496 724 

100 ml 1 10 496 723 

500 ml 1 10 496 726* 




61

Media 

Sabouraud Dextrose Broth 

Products 

Sabouraud Dextrose Broth 

The medium is used for the quantitative 

identification of yeast and 

mold. 

Description 

Peptone in the media is used as a 

nitrogen source for the development of 

fungi. Dextrose acts as an energy 

source for the growth of microorganisms. 

The low pH is favorable for 

the development of fungi, especially 

dermatophytes, but at the same time 

inhibits the development of contaminating 

bacteria from clinical specimens. 


Growth is interpreted as yeast/mold. 

Subculturing and species/group specific 

identification is required. 




Candida albicans ATCC 10231 

incubated for 72 hours at 25–30º C. 



Sterility: 


Sabouraud Dextrose Broth is especially selective 

for yeast and mold due to the low pH conditions. 

Formulation: 


to pH 5.6 ± 0.2 

Peptone 

Dextrose 

For agar add: 

Agar 

10.0 g 

20.0 g 

15.0 g 

Organisms 


C. albicans ATCC 10231 Growth Off-white, 

creamy 

E. coli ATCC 25922 Growth Off-white, 

creamy 

S. cerevisiae ATCC 9763 Growth Off-white 


Sabouraud Dextrose Broth is a modification of dextrose agar described by 

Sabouraud in 1892 for identification of fungi based on their cultural 

characteristics. The medium depended solely upon its acid pH for 

suppression of bacteria. Sabouraud Dextrose Agar, Emmons is a 

modification with a neutral pH and reduced dextrose concentration. 

Historically, the inhibitory action of Sabouraud Dextrose Agar on 

microorganisms other than fungi was enhanced by the addition of agents 

such as tellurite, copper sulfate, bile salts, dyes and antibiotics. 

Sabouraud Dextrose Broth (Fluid Sabouraud Medium) is the liquid 

counterpart of the agar prepared according to the formulation specified in 

the U.S. Pharmacopoeia and National Formulary for sterility testing of 

pharmaceutical products. Used in the quantitative identification of yeast 

and mold. 

Order information Sabouraud Dextrose Media 




100 ml 1 10 496 785* 

500 ml 1 10 496 786* 


100 ml 1 10 496 782* 

500 ml 1 10 496 783* 




62

Products 

Media 

Sabouraud 4 % Dextrose Broth 

Sabouraud 4 % Dextrose Broth 

Used in the quantitative identification 

of yeast, mold and acidophilic 

microorganisms.This culture medium 

complies with the recommendations 

of the United States Pharmacopeia 

and the European Pharmacopeia. 

Description: 

Peptone in the media is used as a 

nitrogen source for the development of 

fungi. Dextrose acts as an energy 

source for the growth of microorganisms. 

The low pH is favorable for the 

development of fungi, especially dermatophytes, 

but at the same time 

inhibits the development of contaminating 

bacteria from clinical specimens. 

The use of Sabouraud media has been 

documented for establishing the microbial 

concentration in cosmetics and 

for the mycological evaluation of food. 


Growth is interpreted as yeast/mold. 

Sub-culturing and species/group 

specific identification is required. 








Sterility: 


Sabouraud 4 % Dextrose Media: Pure culture of 

Candida albicans ATCC 10231. 

Formulation: 


to pH 5.6 ± 0.2 

Peptone 

Dextrose 

10.0 g 

40.0 g 

Organisms 




Order information Sabouraud 4% Dextrose Media 






63

Media 

Standard Methods Agar 

Products 

Standard Methods Agar 

Used for obtaining microbiological 

plate counts from milk and dairy 

products, foods, water and other 

materials of sanitary importance. 

The formulation of this medium 

follows the demands of the Standard 

Methods for the Examination of 

Water and Wastewater, the American 

Public Health Association, the 

American Water Works Association 

and the Water Pollution Control 

Federation in 1992 and the Standard 

Methods for the Examination of Dairy 

Products of the American Public 

Health Association (1985). 

Description: 

All bacteria develop on Standard 

Methods and produce a range of 

different colored and sized colonies. 


It is not possible using Standard 

Methods Agar to presumptively identify 

any bacteria. Identification can only be 

undertaken using traditional microbiology 

techniques following initial colony 

development. 

Organisms 


E. coli ATCC 25922 Growth Yellow to 

cream 

S. aureus ATCC 25923 Growth Yellow to 

cream 





incubated 48 hours at 35º C. 



Sterility: 



Standard Methods Agar is a 

modification of Bowers and 

Hucker Tryptone Glucose Skim 

Milk Agar. It was shown by Yale 

to be more effective in plate 

count procedures on milk and 

dairy products. 

The American Public Health 

Association in their ”Standard 

Methods for the Examination of 

Dairy Products“ recommends 

the use of Standard Methods 

Agar for performance of the 

”Standard Plate Count“ on 

dairy products. 

Standard Methods Agar is the 

same formulation as Plate 

Count Agar recommended by 

the APHA for use in the 

”Standard Plate Count“ on 

water. 

Standard Methods Agar with membrane filter and 

incubated with a pure culture of Escherichia coli 

ATCC 25922. 

Formulation: 


to pH 7.0 ± 0.2 


Yeast extract 

Dextrose 

Agar 

5.0 g 

2.5 g 

1.0 g 

15.0 g 

Same agar as above but incubated with a mix 

culture of Escherichia coli ATCC 25922 and 

Staphylococcus aureus ATCC 25923. Both form 

yellow to creamy colonies. 

Order information Standard Methods Medium 






64

Products 

Media 

Total Count Media with TTC 

Total Count Media with TTC 

Total Count Media with Indicator is 

used for the non-selective development 

and enumeration of all aerobic 

bacteria by the membrane filtration 

method. 

This media complies with the 

specifications given by the APHA for 

the examination of water (1992) and 

for food (1992). 

Description: 

All bacteria develop on Total Count 

Media with indicator and produce a red 

color as a result of the precipitation of 

formazan following the reduction of 

2,3,5-triphenyltetrazolium chloride 

(TTC) by bacteria. The table below 

shows typical organisms which can be 

enumerated with this media: 


It is not possible using Total Count 

Media with Indicator to presumptively 

identify any bacteria. Identification can 

only be undertaken using traditional 

microbiology techniques following 

initial colony development. 








Sterility 


Total Count Media with Indicator: Escherichia coli 

ATCC 25922 and Staphylococcus aureus ATCC 

25923 can be easily detected according to their 

red to pink colonies. 

Formulation: 


to pH 7.0 ± 0.2 


Yeast extract 

Dextrose 

TTC, 1% solution 

10.0 g 

5.0 g 

2.0 g 

10.0 ml 

Organisms 


E. coli ATCC 25922 Growth Pink to red 

S. aureus ATCC 25923 Growth Pink to red 

P. aeruginosa ATCC 10145 Growth Pink to red 

E. faecalis ATCC 29212 Growth Pink to red 

Order information Total Count Media with Indicator 







65

Media 

Trypticase Soy Broth (TSB) 

Products 

Trypticase Soy Broth (TSB) 

Trypticase Soy Broth (TSB) is a 

general-purpose medium used for 

the cultivation of most bacteria. 

Description: 

TSB broth is a medium that will support 

the growth of a wide variety of microorganisms 

including aerobic, facultative 

and anaerobic bacteria and fungi. 


When used as a broth adsorbed onto 

pads TSB will support the development 

of mixed cultures of bacteria and fungi. 

When used in sterility test applications 

turbidity indicates the presence of 

organisms when compared to an 

uninoculated control. 



Positive controls: 


incubation for 24–48 hours at 35º C. 



Sterility: 


Ready to use Tryptic Soy Broth (USP). Not 

inoculated. 

Formulation: 


to pH 7.3 ± 0.2 

Pancreatic digest of casein 




Dextrose 

17.0 g 

3.0 g 

5.0 g 

2.5 g 

2.5 g 

Organisms 


B. subtilis ATCC 6633 Growth 




Order information Trypticase Soy Media (TSB) 


Ampoules 2 ml 50 10 496 160 

Bottled media 50 ml 8 10 496 791* 

100 ml 1 10 496 792* 

100 ml, Septa Cap 1 10 496 750* 

100 ml 1 10 496 707* 





66

Products 

Media 

Trypticase Soy Broth – Single Strength 

Trypticase Soy Broth – Single Strength 

Description: 

General-purpose medium used in 

qualitative procedures for the cultivation 

of fastidious and non-fastidious 

microorganisms. Trypticase Soy Broth – 

Single Strength complies with the demands 

of the DIN Norm 10167 for the 

detection of Escherichia coli serotype 

0157:H7 in foods and FDA-BAM for the 

isolation of enterohemorrhagic Escherichia 

coli (EHEC). In addition the media 

conforms to the formula of the U.S. 

Pharmacopoeia. 







organisms when compared to an uninoculated 

control. 








Sterility: 


Trypticase Soy Broth-Single Strength. Not 

inoculated. 

Formulation: 


to pH 7.3 ± 0.2 





Dextrose 

17.0 g 

3.0 g 

5.0 g 

2.5 g 

2.5 g 

Organisms 







Trypticase Soy Broth was originally developed for detecting the effectiveness 

of sulfonamides against microorganisms such as pneumococci 

without the addition of serum or blood to the medium. Spink and Hamilton 

demonstrated the suitability of the medium for cultivation of aerobic and 

facultative microorganisms including Brucella. Garrision and Hedgecock 

both used the medium to promote the growth of pathogenic fungi. The 

addition of agar to enhance the growth of anaerobic organisms is 

described by Mashima and Ellison and Fredette, Auger, and Forget. 

Sherman and Stark demonstrated that a medium containing 6.5 % NaCl 

could be used for differentiation of group D enterococci from other 

streptococci. 

Order information Trypticase Soy Broth-Single Strength 


Bottled broth 10 ml, Vials 20 10 496 742* 

100 ml 1 10 496 707 

100 ml, wide mouthed 1 10 496 741* 




67

Media 

Trypticase Soy Broth – Double Strength 

Products 

Trypticase Soy Broth – Double Strength 

General-purpose medium used in 

qualitative procedures for the cultivation 

of fastidious and non-fastidious 

microorganisms. This media is also 

recommended for sterility test 

according to U.S. Pharmacopeia. 

Description: 

TSB broth is a medium that will support 

the growth of a wide variety of 

microorganisms including aerobic, 

facultative and anaerobic bacteria and 

fungi. 







organisms when compared to an 

uninoculated control. 



Positive controls: 





Sterility: 


Trypticase Soy Broth (Double Strength). Not 

inoculated. 

Formulation: 


to pH 7.3 ± 0.2 





Dextrose 

34.0 g 

6.0 g 

10.0 g 

5.0 g 

5.0 g 

Organisms 






Trypticase Soy Broth (Double Strength): left vial: 

control, right vial inoculated with Escherichia coli 

ATCC 25922. 

Order information Trypticase Soy Broth (Double Strength) 


Bottled broth 50 ml, W/M bottle 1 10 496 725 

100 ml 1 10 496 708 

68

Products 

Media 

Wallerstein Nutrient Broth (WL) and WL Differential Broth (WLD) 

Wallerstein Nutrient Broth (WL) and 

WLDifferential Broth (WLD) 

WL Nutrient broth is used for the 

determination of microbiological flora 

in brewing and fermentation processes. 

WL differential media suppresses 

the growth of yeast and mold 

and cultivated bacteria encountered 

in brewing and industrial fermentation. 

The medium has the same 

formulation as WL nutrient media but 

has 4 mg of Cyclohexamide per liter 

added. 

Description: 

Use of the medium at pH 5.5 and 

incubation at 25° C will give reliable 

counts for brewer’s yeast. Adjustment 

of the pH to 6.5 and incubation at 30° C 

allows for the selective growth of 

bakers and distillers yeast. 

Interpretation WL Nutrient Broth: 

Media will develop all yeast that 

incubate at the selected temperature 

and pH range. 

WL Nutrient Broth 

Organisms 


Escherichia coli ATCC 25922Growth 

L. fermentum ATCC 9338 Growth 


Interpretation WL Differential Broth: 

When incubated at pH 5.5 estimates of 

beer cocci and lactic rods can be made 

under anaerobic conditions. Estimates 

of acetic acid rods and thermobacteria 

are made under aerobic conditions. 

WL Differential Broth 

Organisms 


Escherichia coli ATCC 25922Growth 

L. fermentum ATCC 9338 Growth 

S. cerevisiae ATCC 9763 Partial to marked inhibition 


incubation conditions WL Nutrient: 


Sacchromyces cerivisiae ATCC 4098, 

incubated 48 hours at 25–30° C. 




Not applicable. 

Sterility: 



incubation conditions WL Differential: 


Lactobacillus plantarum ATCC 8014, 

incubated 48 hours at 35° C. 


Sacchromyces cerivisiae ATCC 4098, 

incubated 48 hours at 25–30° C. 

Sterility: 


Culture of Sacchromyces cerivisiae ATCC 4098 on 

Wallerstein Nutrient Broth (WL). 

Formulation: 


to pH 5.5 ± 0.2 

Casein 

Yeast Extract 

Dextrose 

Monopotassium phospate 

Potassium chloride 

Calcium chloride 


Ferric chloride 

Manganese sulfate 


Additional Component 

for WL Differential Broth: 

Cyclohexamide 

5.0 g 

4.0 g 

50.0 g 

550 mg 

425 mg 

125 mg 

125 mg 

2.5 mg 

2.5 mg 

22 mg 

4 mg 


WL Nutrient Broth has a pH of 5.5 which is optimal for the enumeration of 

brewers’ yeast. In the case of cultivating microorganisms from an alcoholic 

mash, tomato juice should be added to the medium. WL Differential Media 

contains additionally 4 mg of Cyclohexamide per liter. This component 

inhibits the developments of yeast without interfering with the development 

of bacteria generally encountered in beers. Cyclohexamide in WL Differential 

Broth is added to suppress yeast and any mold which may be 

present. 

69

Media 

Wallerstein Nutrient Broth (WL) and WL Differential Broth (WLD) 

Products 


WL Nutrient Agar and WL Differential Agar were developed by GRAY and 

GREEN (1950), to study the microbial population of worts, beers, liquids, 

yeast, and other materials used in fermentation control procedures of the 

brewing industry and other fermentation industries. 

The WL Nutrient Agar is used for the cultivation and enumeration of yeast. 

At pH 5.5 brewers’ yeast will grow unrestricted at 25º C within 24–48 hours. 

For growth of bakers’ and distillers’ yeast, the pH of the medium should be 

adjusted to 6.5 with incubation at 30º C for 2–7 days. Where yeast cells are 

in low number, certain bacteria can be detected. 

The WL Differential Broth is used to determine bacterial counts. GRAY and 

GREEN (1950) recommended using the differential medium to culture 

bacteria normally encountered in brewing; aerobic conditions for acetic 

acid and thermophilic bacteria, anaerobic conditions for lactic bacteria and 

beer cocci. 

Wallerstein Differential Broth (WLD): Cultur of 

Lactobacillus plantarum ATCC 8014. 

Order information WL Nutrient Media 


Ampoules 2 ml 50 10 496 108 




Order information WL Differential Media 


Ampoules 2 ml 50 10 496 109 

Bottled Media 50 ml 8 10 496 717* 




70

Cetrimide Broth

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