35th NPS abstract book

More documents

Recommendations

Info

P27 Forest tree GnpIS: an information system dedicated to forest tree genetics, genomics and phenomics C. MICHOTEY 1 , C.ANGER 2 , F. EHRENMANN 4 , O. ROGIER 3 , V. JORGE 3 , C. POMMIER 1 , C. BASTIEN 3 , C. PLOMION 4 , C. PICHOT 5 , H. QUESNEVILLE 1 and D. STEINBACH 1 1 INRA, UR1164 - URGI (Unité de Recherche en Génomique-Info), route de Saint-Cyr – RD 10, 78026 Versailles cedex, France ; 2 INRA, UE0995 - GBFOR (Génétique et Biomasse Forestière ORléans), 2163 avenue de la Pomme de Pin, CS 40001 Ardon, 45075 Orléans cedex, France; 3 INRA, UR0588 - AGPF (Amélioration, Génétique et Physiologie Forestières), 2163 avenue de la Pomme de Pin, CS 40001 Ardon, 45075 Orléans cedex, France ; 4 INRA, UMR1202 - BIOGECO (BIOdiversité, GEnes et COmmunautés), 69 route d’Arcachon, 33612 Cestas cedex, France; 5 INRA, UR0629 - URFM (Ecologie des Forêts Méditerranéennes), 228 route de l’Aérodrome, 84914 Avignon, France GnpIS is a multispecies integrative information system dedicated to plants and fungi pests. It integrates and links genetic, genomic, phenomic and environmental data into a single environment, allowing researchers to store, query and explore information from different angles. The Ecology division of the French National Institute for Agricultural Research (INRA) uses GnpIS as its referential information system to manage forest tree genetic, genomic and phenomic data. The forest tree resources are accessible through the GnpIS web portal (https://urgi.versailles.inra.fr/gnpis/). Its main entry point is a google-like search for data discovery. This bird’s eye view allows navigation through the data with specific querying tools. It is regularly improved with new functionalities answering specific needs raised by scientists and is released several times a year. Data are supplied by local sources (files, databases …) produced and managed by research teams working on forest trees. Pine, spruce and oak data have been already integrated into this forest information system and we are giving access to poplar genetic, phenomic and genomic data (https://urgi.versailles.inra.fr/Species/Forest-trees/Database-overview). Integration of the data produced within the common garden network (over 1,000 trials with genotypes gathered from ~15 species) is in progress. 45
P28 A developmental and genetic study of petal-less neotropical poppy trees N. PABÓN-MORA 1* , C. ARANGO OCAMPO 1 , J. F. ALZATE 2 and F. GONZÁLEZ 3 1 Instituto de Biología, Universidad de Antioquia, AA 1226; 2 Centro de Secuenciación Genómico Nacional, Universidad de Antioquia, AA 1226; 3 Instituto de Ciencias Naturales, Universidad Nacional de Colombia, AA 7495 Flowers of most Papaveraceae (760 spp.) exhibit a dimerous groundplan with 2 deciduous sepals, 4 petals, many stamens and 2-8 carpels. The genera Bocconia (9 spp.) and Macleaya (2 spp.) are atypical in their tree- or shrubby habit, and in having perianth-less flowers. By comparing flower development in B. frutescens, M. cordata and its closely related species Stylophorum diphyllum, we show that petal-to-stamen homeosis occurs early in development, but different ontogenetic pathways underlie petal-loss in Bocconia and Macleaya. In order to explore the genetic basis of apetaly, we generated a floral transcriptome of B. frutescens and identified all MADS-box transcription factors that are part of the ABCE genetic model of floral development. We present data on the evolution of these genes in the Ranunculales and their expression in dissected floral organs, leaves and fruits in B. frutescens We also propose that the lack of expression of an AP3-3 ortholog is responsible for such apetaly, as it occurs in species of Ranunculaceae. We identified local duplications in FUL-like and PI genes likely resulting in pseudogenization and neofunctionalization, respectively. Based on the number of copies and patterns of expression, we propose a modified ABCE model for the petal-less poppy trees. P29 Microevolutionary patterns of a resistance mechanism in white spruce G.J. PARENT 1 , I. GIGUÈRE 1 and J.J. MACKAY 1, 2 1 Centre d’étude de la Forêt, Département des Sciences du Bois et de la Forêt, Université Laval, G1A 1V6, Québec, Canada; 2 Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, UK The accumulation of two foliar acetophenone compounds (piceol and pungenol) plays a key role in the defense of white spruce (Picea glauca) against spruce budworm (SBW, Choristoneura fumiferana). Variability in the expression of a beta-glucosidase, PGβGLU-1, responsible for their release was linked to SBW resistance. This novel resistance mechanism is constitutive, genetically transmissible and variable in the natural white spruce population. We were interested in characterizing its microevolutionary patterns to better understand its importance. Levels of Pgβglu-1 transcripts and toxic acetophenones were measured in white spruce from multiple provenances and preliminary results indicated a clear geographic pattern. Foliage expression of Pgβglu-1 was lower in provenances south of the 48th parallel. This result may be accounted for by lower selection pressure exerted by SBW associated with a reduced survival rate at the southern limit of its distribution. However, concentrations of piceol and pungenol in white spruce foliage did not follow the geographic pattern found for gene expression. This suggests that control of these specific acetophenone compounds may also be affected by other factors than the expression of Pgβglu-1. We are currently working to find other members of the biochemical pathway and regulatory network through association testing and other genome analysis methods. 46
Page 1 and 2: 16-17 June 2015 Arnold Arboretum of
Page 3 and 4: Table of Contents Information for D
Page 5 and 6: Discussion session More information
Page 7 and 8: 16:45-17:00 Selected poster abstrac
Page 9 and 10: Tour Groups Tuesday (15:15-16:15) T
Page 11 and 12: Wednesday Discussion Session Discus
Page 13 and 14: Speaker Abstracts * S=speaker abstr
Page 15 and 16: Microevolution in Populus trichocar
Page 17 and 18: Comparative and ecological genomics
Page 19 and 20: Drivers of diversity in tropical fo
Page 21 and 22: Ginkgo: An evolutionary and cultura
Page 23 and 24: Transcriptional switches controllin
Page 25 and 26: Transcriptional networks regulating
Page 27 and 28: Computational tools and resources f
Page 29 and 30: Amselem, Joelle Buggs, Richard Bull
Page 31 and 32: Poster Abstracts Poster abstracts a
Page 33 and 34: P3 Using direct amplification and n
Page 35 and 36: P7 Distributed control constrains e
Page 37 and 38: P11 Gene expression and natural sel
Page 39 and 40: P15 How has our understanding of th
Page 41 and 42: P18 Visualizing molecular changes a
Page 43 and 44: P21 Nuclear microsatellite markers
Page 45: P25 The effects of selective breedi
Page 49 and 50: P31 Wood formation in the real worl
Page 51 and 52: P35 Population-scale characterisati
Page 53 and 54: P39 Conifer’s comparative genomic
Page 55 and 56: P43 Molecular control of vasculatur
Page 57 and 58: Participants Amselem, Joelle INRA j
Page 59 and 60: Menon, Mitra University of Virginia
Page 61: HUNNEWELL VISITOR CENTER A RBORWAY

35th NPS abstract book

Create successful ePaper yourself

Delete template?

Save as template?